PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32097726-8 2020 IC50 values were determined to be 0.018 and 0.029 mM for potato PPO with curcumin and quercetin inhibitors with catechol as a substrate, respectively. catechol 112-120 catechol oxidase B, chloroplastic Solanum tuberosum 64-67 30209938-2 2018 The aim of this study was to develop surface-enhanced Raman scattering (SERS) approach for accurate determination of PPO activity in fruit and vegetables using the reduction in SERS intensity of catechol in reaction medium. catechol 195-203 catechol oxidase B, chloroplastic Solanum tuberosum 117-120 30209938-3 2018 Within a certain catechol concentration, when a purified PPO solution was analyzed, the reduction in SERS intensity (Delta I) was linear to PPO activity ( Ec) in a wide range of 500-50 000 U/L, and a linear regression equation of log Delta I/Delta t = 0.6223 log Ec + 0.8072, with a correlation coefficient of 0.9689 and a limit of detection of 224.65 U/L, was obtained. catechol 17-25 catechol oxidase B, chloroplastic Solanum tuberosum 57-60 30209938-3 2018 Within a certain catechol concentration, when a purified PPO solution was analyzed, the reduction in SERS intensity (Delta I) was linear to PPO activity ( Ec) in a wide range of 500-50 000 U/L, and a linear regression equation of log Delta I/Delta t = 0.6223 log Ec + 0.8072, with a correlation coefficient of 0.9689 and a limit of detection of 224.65 U/L, was obtained. catechol 17-25 catechol oxidase B, chloroplastic Solanum tuberosum 140-143 17417695-2 2007 Under optimized conditions (formaldehyde 20 mg/ml, PPO 4 mg/ml and pH 7.0), the activity of immobilized PPO was 1370 U/g and its Km value for catechol was 12 mM at 25 degrees C. The highest activity of immobilized enzyme was at pH 7.4. catechol 142-150 catechol oxidase B, chloroplastic Solanum tuberosum 104-107 34681535-7 2021 AA only slows mechanism-based inactivation of PPO induced by catechol, possibly owing to the prevention of quinone formation. catechol 61-69 catechol oxidase B, chloroplastic Solanum tuberosum 46-49 34681535-9 2021 The problem associated with AA in PPO assay could be circumvented by using ascorbate oxidase to remove AA when catechol is the primary substrate or by using chlorogenic acid as the primary substrate. catechol 111-119 catechol oxidase B, chloroplastic Solanum tuberosum 34-37 32447731-8 2020 PPO and a chromophore reagent (3-methyl-2-benzothiazolinone hydrazine) generated a range of color in the presence of phenolic compounds (catechol, phenol, p-cresol, 4-methyl catechol) within 15 min, and limit of detection was found to be 0.5 muM. catechol 137-145 catechol oxidase B, chloroplastic Solanum tuberosum 0-3