PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16446809-3 2006 The prepared 1,4-benzodiazepine-2,5-dione compounds are evaluated for endothelin receptor antagonism by a functional assay that measures the inhibitory activity against the change of intramolecular calcium ion concentration induced by endothelin-1. Calcium 198-205 endothelin 1 Homo sapiens 235-247 14638730-14 2003 The ET(A) receptor was linked to calcium mobilization as ET-1 produced an increase in intracellular calcium release, and this increase was blocked with a selective ET(A) receptor antagonist. Calcium 33-40 endothelin 1 Homo sapiens 57-61 16042982-9 2005 Moreover, using the Fura-2 calcium measurement technique, our results showed that in left ventricular EECs, both ET(A) and ET(B) receptor activation mediated the effect of ET-1 on intracellular calcium, whereas in right ventricular EECs, this effect was solely mediated by the ET(A) receptor. Calcium 27-34 endothelin 1 Homo sapiens 172-176 16042982-3 2005 In this study, we verified if the distribution and density of ET-1 and its receptors could be different in right as compared to left ventricular EECs, and whether this difference may affect ET-1-induced increase of intracellular calcium. Calcium 229-236 endothelin 1 Homo sapiens 62-66 16042982-3 2005 In this study, we verified if the distribution and density of ET-1 and its receptors could be different in right as compared to left ventricular EECs, and whether this difference may affect ET-1-induced increase of intracellular calcium. Calcium 229-236 endothelin 1 Homo sapiens 190-194 15331591-1 2004 A trigger zone for calcium signaling by nicotinic acid adenine dinucleotide phosphate and endothelin-1. Calcium 19-26 endothelin 1 Homo sapiens 90-102 14966371-4 2004 However, a phospholipase C inhibitor, U73122, also eliminated the intracellular calcium mobilization induced by ET-1, but had no effect on ET-1-stimulated glucose uptake. Calcium 80-87 endothelin 1 Homo sapiens 112-116 15055432-6 2004 These data demonstrate that endothelin-1 induces calcium-dependent vasoconstriction in porcine pulpal arterioles, with extraluminal application more potent, which seems to reflect the possible modulation of vascular endothelium in the control of vascular tone. Calcium 49-56 endothelin 1 Homo sapiens 28-40 14638730-14 2003 The ET(A) receptor was linked to calcium mobilization as ET-1 produced an increase in intracellular calcium release, and this increase was blocked with a selective ET(A) receptor antagonist. Calcium 100-107 endothelin 1 Homo sapiens 57-61 14609525-1 2003 Treatment of brain microvessels with Endothelin-1 evoked a decrease in ethanolamine plasmalogen levels by calcium-independent phospholipase A(2). Calcium 106-113 endothelin 1 Homo sapiens 37-49 14959449-3 2003 Insulin increases endothelin-1 (ET-1) release from endothelial cells modulating smooth muscle calcium levels and elevating force generated by femoral and coronary arteries. Calcium 94-101 endothelin 1 Homo sapiens 18-30 14959449-3 2003 Insulin increases endothelin-1 (ET-1) release from endothelial cells modulating smooth muscle calcium levels and elevating force generated by femoral and coronary arteries. Calcium 94-101 endothelin 1 Homo sapiens 32-36 12887970-0 2003 Sodium-calcium exchange influences the response to endothelin-1 in lens epithelium. Calcium 7-14 endothelin 1 Homo sapiens 51-63 12887970-1 2003 Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Calcium 155-162 endothelin 1 Homo sapiens 117-129 12887970-1 2003 Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Calcium 155-162 endothelin 1 Homo sapiens 131-135 12887970-3 2003 ET-1 (100 nM) caused cytoplasmic calcium to increase transiently to approximately 250 nM from a baseline of approximately 65 nM. Calcium 33-40 endothelin 1 Homo sapiens 0-4 12887970-5 2003 Both the peak and plateau component of the ET-1 calcium response were abolished by PD145065, an ET receptor antagonist, and by cyclopiazonic acid (CPA) (10 microM). Calcium 48-55 endothelin 1 Homo sapiens 43-47 12887970-11 2003 Taken together findings suggest inhibition of sodium-calcium exchange increases the magnitude of the receptor-initiated store-release phase of the ET-1 calcium signaling response as the result of impaired calcium clearance from the cytoplasm. Calcium 53-60 endothelin 1 Homo sapiens 147-151 12887970-11 2003 Taken together findings suggest inhibition of sodium-calcium exchange increases the magnitude of the receptor-initiated store-release phase of the ET-1 calcium signaling response as the result of impaired calcium clearance from the cytoplasm. Calcium 152-159 endothelin 1 Homo sapiens 147-151 12887970-11 2003 Taken together findings suggest inhibition of sodium-calcium exchange increases the magnitude of the receptor-initiated store-release phase of the ET-1 calcium signaling response as the result of impaired calcium clearance from the cytoplasm. Calcium 152-159 endothelin 1 Homo sapiens 147-151 11715356-8 2001 Calcium antagonists counteract the effects of angiotensin II and endothelin-1 at the level of vascular smooth muscle by reducing Ca2+ inflow and facilitating the vasodilator effects of nitric oxide. Calcium 0-7 endothelin 1 Homo sapiens 65-77 14517584-0 2003 Differential effects of endothelin-1 on calcium mobility in cultured porcine pigment epithelial cells of iris and retina. Calcium 40-47 endothelin 1 Homo sapiens 24-36 12147606-6 2002 ET-1-induced intracellular calcium ([Ca2+]i) in hONAs was measured by fura-2 imaging. Calcium 27-34 endothelin 1 Homo sapiens 0-4 12063458-2 2002 Recent evidence suggests that cardiomyocytes might also produce endothelin-1, which might directly impair myocyte contractility by increasing intracellular calcium levels. Calcium 156-163 endothelin 1 Homo sapiens 64-76 11853172-2 2002 When ET-1 acts on NHMC via the endothelin B receptor (ET(B)R) on their cell surface, mobilization of intracellular calcium is induced, which is followed by activation of Raf-1 located upstream of mitogen activated protein kinase (MAPK). Calcium 115-122 endothelin 1 Homo sapiens 5-9 11853172-7 2002 Taken together, these findings indicate that an extract of A. officinalis inhibits both the secretion of ET-1 from NHKC and the action of ET-1 on NHMC mainly by suppressing the ET-1-induced calcium mobilization without the modification of IP3 production, which in turn suggests that this extract is a useful ingredient for a whitening agent. Calcium 190-197 endothelin 1 Homo sapiens 105-109 11853172-7 2002 Taken together, these findings indicate that an extract of A. officinalis inhibits both the secretion of ET-1 from NHKC and the action of ET-1 on NHMC mainly by suppressing the ET-1-induced calcium mobilization without the modification of IP3 production, which in turn suggests that this extract is a useful ingredient for a whitening agent. Calcium 190-197 endothelin 1 Homo sapiens 138-142 11853172-7 2002 Taken together, these findings indicate that an extract of A. officinalis inhibits both the secretion of ET-1 from NHKC and the action of ET-1 on NHMC mainly by suppressing the ET-1-induced calcium mobilization without the modification of IP3 production, which in turn suggests that this extract is a useful ingredient for a whitening agent. Calcium 190-197 endothelin 1 Homo sapiens 138-142 11847185-10 2002 These data suggest that the preproendothelin-1 gene polymorphism is associated with a higher potentiating effect of endothelin-1 and angiotensin II, probably in relation with higher calcium sensitivity. Calcium 182-189 endothelin 1 Homo sapiens 28-46 11847185-10 2002 These data suggest that the preproendothelin-1 gene polymorphism is associated with a higher potentiating effect of endothelin-1 and angiotensin II, probably in relation with higher calcium sensitivity. Calcium 182-189 endothelin 1 Homo sapiens 34-46 11743235-4 2002 Cells responded to endothelin-1 and a variety of other vasoconstrictors with rises in cytoplasmic calcium ([Ca2+]c). Calcium 98-105 endothelin 1 Homo sapiens 19-31 11276396-3 2001 Additionally, a portion of alpha-adrenergic, serotonergic, and endothelin-1-induced contraction is partially mediated by the calcium-independent activation of the small G-protein RhoA and of a downstream target, Rho-kinase. Calcium 125-132 endothelin 1 Homo sapiens 63-75 11274080-13 2001 ET-1 caused an increase of cytoplasmic calcium concentration in cultured porcine lens epithelium. Calcium 39-46 endothelin 1 Homo sapiens 0-4 11274080-14 2001 The cytoplasmic calcium response to ET-1 was inhibited by PD145065 and genistein. Calcium 16-23 endothelin 1 Homo sapiens 36-40 12839276-0 2003 Activation of sarcolemma and nuclear membranes ET-1 receptors regulates transcellular calcium levels in heart and vascular smooth muscle cells. Calcium 86-93 endothelin 1 Homo sapiens 47-51 12839276-6 2003 This effect of extracellular ET-1 was blocked by removal of extracellular calcium. Calcium 74-81 endothelin 1 Homo sapiens 29-33 12547713-7 2003 These results suggest that ET-1 suppresses Ca++-Mg++-ATPase activity and Ca++ pump-ATPase expression on the plasma membrane of sinusoidal endothelial fenestrae, thereby attenuating the extrusion of intracytoplasmic free Ca++ into the extracellular space, leading to an increased concentration of intracytoplasmic free calcium ions and contraction of sinusoidal endothelial fenestrae. Calcium 318-325 endothelin 1 Homo sapiens 27-31 11847185-3 2002 At subthreshold concentrations, endothelin-1 and angiotensin II induce a potentiation of alpha-adrenergic-dependent vascular tone caused by an increased sensitivity of the contractile apparatus to calcium. Calcium 197-204 endothelin 1 Homo sapiens 32-44 11078437-5 2000 Finally, our results suggest that cytosolic ET-1 may play a role in preventing HVSMC nuclear calcium overload, thus protecting the cells from apoptosis. Calcium 93-100 endothelin 1 Homo sapiens 44-48 11528212-0 2001 Modulation of endothelin-1-induced cytosolic free calcium mobilization and mitogen-activated protein kinase activation by erythropoietin in vascular smooth muscle cells. Calcium 50-57 endothelin 1 Homo sapiens 14-26 11528212-2 2001 We investigated whether rHuEPO has modulative effects on the endothelin-1 (ET-1)-induced elevation of cytosolic free calcium concentration ([Ca2+]i) and mitogen-activated protein (MAP) kinase activation in vascular smooth muscle cells (VSMC). Calcium 117-124 endothelin 1 Homo sapiens 61-73 11528212-2 2001 We investigated whether rHuEPO has modulative effects on the endothelin-1 (ET-1)-induced elevation of cytosolic free calcium concentration ([Ca2+]i) and mitogen-activated protein (MAP) kinase activation in vascular smooth muscle cells (VSMC). Calcium 117-124 endothelin 1 Homo sapiens 75-79 11078385-9 2000 Functional studies using microfluorimetry (fura-2 method) showed comparable biphasic calcium signals induced by 10 nm ET-1, ET-3 and S6c. Calcium 85-92 endothelin 1 Homo sapiens 118-122 10612435-1 1999 In previous studies in porcine granulosa cell cultures, endothelin-1 (ET-1) was shown to inhibit FSH-stimulated cAMP and progesterone accumulation, and to increase inositol phosphate formation and cytosolic calcium ion concentration. Calcium 207-214 endothelin 1 Homo sapiens 56-68 10749886-8 2000 Both RGS3 and RGS3T bound to endogenous Galpha(q/11) and inhibited endothelin-1-stimulated calcium mobilization and mitogen-activated protein kinase activity to a similar extent. Calcium 91-98 endothelin 1 Homo sapiens 67-79 11078432-4 2000 The sustained increase of cytosolic and nuclear Ca2+ by ET-1 in both EEC preparations was completely blocked by the calcium chelator ethylene glycol-bis (beta-aminoethylether)-N,N,N",N"-tetra-acetic acid (EGTA) but was insensitive to the L-type Ca2+ channel blocker, nifedipine. Calcium 116-123 endothelin 1 Homo sapiens 56-60 10855694-9 2000 Further studies should be conducted to elucidate the physiological role of endothelins as possible autocrine, paracrine, or endocrine factors in calcium and bone metabolism. Calcium 145-152 endothelin 1 Homo sapiens 75-86 10612435-1 1999 In previous studies in porcine granulosa cell cultures, endothelin-1 (ET-1) was shown to inhibit FSH-stimulated cAMP and progesterone accumulation, and to increase inositol phosphate formation and cytosolic calcium ion concentration. Calcium 207-214 endothelin 1 Homo sapiens 70-74 10532691-4 1999 Furthermore, ET-1 stimulated protein synthesis (maximum 73 +/- 32% increase in [3H]leucine incorporation by 10(-7) M (P < 0.05)), as well as triggering intracellular calcium transients in human cSMC, as visualised under fura-2 fluorescence microscopy. Calcium 169-176 endothelin 1 Homo sapiens 13-17 10532691-5 1999 The selective ET(A) receptor antagonist BQ123 inhibited the increases in DNA synthesis, cell number, protein synthesis and intracellular calcium concentration in response to ET-1, whereas the ET(B) receptor antagonist BQ788 had no such effects. Calcium 137-144 endothelin 1 Homo sapiens 174-178 10362619-0 1999 Mechanism of endothelin-1-(1-31)-induced calcium signaling in human coronary artery smooth muscle cells. Calcium 41-48 endothelin 1 Homo sapiens 13-25 9893159-4 1999 Ca2+ store depletion by thapsigargin, endothelin-1, or bradykinin activated calcium entry pathways. Calcium 76-83 endothelin 1 Homo sapiens 38-50 9973602-0 1999 Underexpression of the 43 kDa inositol polyphosphate 5-phosphatase is associated with spontaneous calcium oscillations and enhanced calcium responses following endothelin-1 stimulation. Calcium 132-139 endothelin 1 Homo sapiens 160-172 10340841-8 1999 Calcium antagonists counteract angiotensin II and endothelin-1 at the level of vascular smooth muscle by reducing the inflow of Ca2+ and facilitating the vasodilator effects of NO. Calcium 0-7 endothelin 1 Homo sapiens 50-62 9882452-6 1999 Endothelin-1-induced mobilization of calcium from intracellular stores was also diminished by TNFalpha. Calcium 37-44 endothelin 1 Homo sapiens 0-12 9858594-12 1999 The effect of endothelin-1 was also mimicked by calcium ionophore A23187, suggesting the importance of Ca2+ in the mechanism of redistribution of RGS3. Calcium 48-55 endothelin 1 Homo sapiens 14-26 10503767-1 1999 Exposure of C6 glioma cells to endothelin-1 (ET-1) caused dose-dependent (10(-11) M to 10(-7) M) increments in intracellular calcium concentration ([Ca2+]i) and c-fos mRNA expression (4.5-fold) that were abolished by the endothelinA receptor antagonist, BQ610, and by inhibition of phospholipase C with U73122. Calcium 125-132 endothelin 1 Homo sapiens 31-43 10503767-1 1999 Exposure of C6 glioma cells to endothelin-1 (ET-1) caused dose-dependent (10(-11) M to 10(-7) M) increments in intracellular calcium concentration ([Ca2+]i) and c-fos mRNA expression (4.5-fold) that were abolished by the endothelinA receptor antagonist, BQ610, and by inhibition of phospholipase C with U73122. Calcium 125-132 endothelin 1 Homo sapiens 45-49 9869001-2 1998 Angiotensin II, endothelin 1, and other peptides, as well as catecholamines, may contribute to the altered structure and function of small arteries, in part through their effects on intracellular calcium. Calcium 196-203 endothelin 1 Homo sapiens 16-28 9354305-4 1997 LDL-cholesterol also potentiates responses to vasoconstrictors such as noradrenaline and endothelin-1 in the absence of endothelium, possibly by enhancing calcium influx into vascular smooth muscle cells. Calcium 155-162 endothelin 1 Homo sapiens 89-101 9573527-8 1998 Since an increase in intracellular calcium ([Ca2+]i) was shown to be necessary for ET-1-induced activation of JNK in mesangial cells, we measured [Ca2+]i using fura-2. Calcium 35-42 endothelin 1 Homo sapiens 83-87 9613405-2 1997 Calcium antagonists have suppressed ET-1 release in some studies. Calcium 0-7 endothelin 1 Homo sapiens 36-40 10453541-3 1997 CONCLUSION: It was also indicated that extracellular calcium influx and calmodulin activity were necessary to the secretion of ET-1. Calcium 53-60 endothelin 1 Homo sapiens 127-131 9432637-3 1997 Endothelin-1 through second messengers mobilises the intracellular calcium storage. Calcium 67-74 endothelin 1 Homo sapiens 0-12 9466679-0 1997 Contraction and intracellular calcium-ion elevation of cultured human aortic smooth muscle cells by endothelin-1, vasoactive intestinal contractor (VIC) and the derivatives. Calcium 30-37 endothelin 1 Homo sapiens 100-112 9466679-2 1997 Contraction of cultured human VSM, isolated from human fetal aortic segments, was induced within 1 min after the treatment with ET-1 (100 nM) as seen in the changes of cytosolic calcium-ion localization. Calcium 178-185 endothelin 1 Homo sapiens 128-132 9457516-0 1998 Modulation of endothelin-1-induced contractions by magnesium/calcium in porcine ciliary arteries. Calcium 61-68 endothelin 1 Homo sapiens 14-26 9457516-8 1998 In endothelin-1-precontracted vessels, magnesium evoked complete concentration-dependent relaxations (pD2 = 3.1 +/- 0.1), which were shifted to the right by increasing extracellular concentrations of calcium (2.5 vs 5 mM, P < 0.05). Calcium 200-207 endothelin 1 Homo sapiens 3-15 9605596-9 1998 Calcium antagonists inhibit the effects of Ang I and endothelin-1 (ET-1) at the level of vascular smooth muscle by reducing Ca2+ inflow and facilitating the vasodilator effects of NO. Calcium 0-7 endothelin 1 Homo sapiens 53-65 9236133-9 1997 Co-incubations of ET-1 and CAS 754 resulted in a significant decrease in cell proliferation, protein synthesis, and collagen levels (-23+/-2% v control, 90+/-5% v control, and 63+/-3% v control, respectively) compared to ET-1 alone. Calcium 27-30 endothelin 1 Homo sapiens 221-225 9313622-8 1997 Calcium antagonists counteract angiotensin II and endothelin-1 at the level of vascular smooth muscle by reducing Ca2+ inflow and facilitating the vasodilator effects of NO. Calcium 0-7 endothelin 1 Homo sapiens 50-62 9397288-9 1997 Whereas nitrates partly substitute deficient endogenous nitric oxide, calcium antagonists counteract angiotensin II and endothelin-1 at the level of vascular smooth muscle by reducing Ca2+ inflow and facilitating the vasodilator effects of nitric oxide. Calcium 70-77 endothelin 1 Homo sapiens 120-132 9067893-8 1997 In contrast, ET-1-induced activation of JNK was significantly reduced by calcium chelation (with BAPTA/AM and EGTA). Calcium 73-80 endothelin 1 Homo sapiens 13-17 9034753-6 1997 Calcium antagonists have been shown to enhance the effects of NO, and inhibit those of ET-1, on vascular smooth muscle cells. Calcium 0-7 endothelin 1 Homo sapiens 87-91 8732499-2 1996 Vasopressin and endothelin-1 (100 nM) evoked an initial peak followed by a smaller sustained rise of [Ca2+]i in the presence of extracellular calcium [Ca2+]o. Calcium 142-149 endothelin 1 Homo sapiens 16-28 8764613-0 1996 Endothelin-1 increases arachidonic acid release in C6 glioma cells through a potassium-modulated influx of calcium. Calcium 107-114 endothelin 1 Homo sapiens 0-12 8764613-2 1996 Et-1 activation was concentration dependent and was inhibited by chelation of extracellular calcium. Calcium 92-99 endothelin 1 Homo sapiens 0-4 8764613-8 1996 The results therefore suggest that Et-1 activation of PLA2 in this cell type requires calcium influx dependent on K+ efflux. Calcium 86-93 endothelin 1 Homo sapiens 35-39 8764168-0 1996 Attenuation of endothelin-1-induced calcium response by tyrosine kinase inhibitors in vascular smooth muscle cells. Calcium 36-43 endothelin 1 Homo sapiens 15-27 8631621-5 1996 RESULTS: Endothelin-1 (ET-1) stimulated PLC (mean EC50 = 335 pM) and activated calcium mobilization in HCM cells. Calcium 79-86 endothelin 1 Homo sapiens 9-21 8631621-5 1996 RESULTS: Endothelin-1 (ET-1) stimulated PLC (mean EC50 = 335 pM) and activated calcium mobilization in HCM cells. Calcium 79-86 endothelin 1 Homo sapiens 23-27 8886236-1 1996 A model is proposed to describe the electrical activity and intracellular calcium dynamics of vascular smooth muscle cells (SMC) induced by endothelin (ET1). Calcium 74-81 endothelin 1 Homo sapiens 152-155 8886236-8 1996 The model simulations show that (a) in cells without a CICR mechanism, short-lasting stimulation by ET1 elicits higher membrane potential and Ca2+ than long-lasting stimulation; (b) in cells with or without a CICR mechanism, a reduction of normal membrane capacitance (1 muf/cm2) results in either significant and sustaining or oscillatory membrane potential and intracellular calcium concentration. Calcium 377-384 endothelin 1 Homo sapiens 100-103 8732499-17 1996 These results provide evidence that calcium entry and membrane depolarization elicited by vasopressin or endothelin-1 are mediated by a receptor-mediated Ca(2+)-permeable non-selective cation channel in aortic smooth muscle cells. Calcium 36-43 endothelin 1 Homo sapiens 105-117 7631046-6 1995 ET-1 acutely activates phospholipase A2 through phosphorylation and acute increases of intracellular calcium. Calcium 101-108 endothelin 1 Homo sapiens 0-4 8719260-4 1995 We found that the response to ET-1 in the placental arteries from women with normal pregnancies was reduced by 20% in the absence of extracellular calcium. Calcium 147-154 endothelin 1 Homo sapiens 30-34 8719260-9 1995 We conclude that the ET-1-induced contractile response in the human placental artery is dependent on influx of extracellular calcium as well as mobilization of calcium from intracellular stores. Calcium 125-132 endothelin 1 Homo sapiens 21-25 8719260-9 1995 We conclude that the ET-1-induced contractile response in the human placental artery is dependent on influx of extracellular calcium as well as mobilization of calcium from intracellular stores. Calcium 160-167 endothelin 1 Homo sapiens 21-25 8606524-0 1995 Endothelin-1 release by erythropoietin involves calcium signaling in endothelial cells. Calcium 48-55 endothelin 1 Homo sapiens 0-12 8588187-0 1995 Endothelin-1 evoked an increase and oscillations in cytosolic calcium concentration in adherent single human platelets and increased GMP-140 (P-selectin) in platelet suspension. Calcium 62-69 endothelin 1 Homo sapiens 0-12 8588187-1 1995 The effect of endothelin-1 (ET-1) on cytosolic calcium ion concentration ([Ca2+]i) in adherent single human blood platelet was determined by fluorescence digital imaging microscopy using Fura-2 as calcium probe. Calcium 47-54 endothelin 1 Homo sapiens 14-26 8588187-1 1995 The effect of endothelin-1 (ET-1) on cytosolic calcium ion concentration ([Ca2+]i) in adherent single human blood platelet was determined by fluorescence digital imaging microscopy using Fura-2 as calcium probe. Calcium 47-54 endothelin 1 Homo sapiens 28-32 7658593-5 1995 RESULTS: Endothelin-1 elicited a transient and concentration-dependent increase in cytosolic and nuclear calcium levels in cells from both patient populations. Calcium 105-112 endothelin 1 Homo sapiens 9-21 7658593-6 1995 However, while the peak amplitudes of the ET-1-induced cytosolic calcium increases were similar in cells from the 2 patient populations, the observed increases in nuclear calcium levels were much more pronounced in cells from patients with organic erectile dysfunction than in patients with documented erections. Calcium 65-72 endothelin 1 Homo sapiens 42-46 7658593-7 1995 CONCLUSIONS: Endothelin-1, by virtue of its ability to increase both cytosolic and nuclear calcium levels, may serve not only as a critical determinant of corporal tissue tone, but also as a modulator of cellular proliferation and phenotypic expression. Calcium 91-98 endothelin 1 Homo sapiens 13-25 9816080-1 1995 The production of endothelin 1 (ET-1) and its receptor-mediated actions on calcium signaling and growth responses were analyzed in human ovarian carcinoma cells. Calcium 75-82 endothelin 1 Homo sapiens 18-30 8660299-3 1996 The ET(A) receptor subtype-sensitive antagonist BQ123(50 nM) or pertussis toxin (100 ng/ml) significantly suppressed the ET-1-induced intracellular calcium mobilization, indicating the presence of pertussis toxin-sensitive G-protein-coupled ET(A) receptors. Calcium 148-155 endothelin 1 Homo sapiens 121-125 8742913-5 1996 After nisoldipine treatment the plasma ET-1 levels were significantly lower at rest and during exercise compared with those revealed before calcium antagonist treatment. Calcium 140-147 endothelin 1 Homo sapiens 39-43 8742913-6 1996 In conclusion our results suggest that in patients with chronic stable angina pectoris the treatment with calcium antagonist nisoldipine reduced ischemia and plasma ET-1 levels. Calcium 106-113 endothelin 1 Homo sapiens 165-169 8772525-8 1996 We therefore conclude that in rabbit airways ET-1 activates ETB receptors, triggers the influx of extracellular calcium through voltage-dependent channels, and induces a contractile response that is, in part, dependent upon stimulation of PKC. Calcium 112-119 endothelin 1 Homo sapiens 45-49 8772525-10 1996 Finally, in human airways, the contractile response to ET-1, while independent of extracellular calcium influx, is dependent upon PKC activation after binding of the peptide to ETB receptors. Calcium 96-103 endothelin 1 Homo sapiens 55-59 8587330-7 1995 These observations demonstrate that thrombin stimulates the preproET-1 gene in human endothelial cells through calcium-dependent activation of CaM kinase and protein tyrosine kinases, and that calcineurin may also participate in regulation of the prepro ET-1 gene. Calcium 111-118 endothelin 1 Homo sapiens 60-70 8588187-4 1995 The ET-1 induced increase in [Ca2+]i was suppressed by 1 mM EGTA, a calcium chelating agent in the medium. Calcium 68-75 endothelin 1 Homo sapiens 4-8 8588187-8 1995 Therefore, we suggested that ET-1 increased [Ca2+]i in platelets by both calcium influx and IP3 mediated Ca2+ release resulting in activation and release of GMP-140. Calcium 73-80 endothelin 1 Homo sapiens 29-33 7748184-3 1995 In cell-attached patches, addition of ANG II (500 nM) or ET-1 (50 nM) to the bath inhibited the KCa-channel activated by the calcium ionophore A23187 (10-20 microM). Calcium 125-132 endothelin 1 Homo sapiens 57-61 7537639-8 1995 Endothelin-1 at 10(-9) mol/l significantly decreased (P < 0.01) thrombin-stimulated aggregation from 81.4 +/- 1.5% (in the absence of endothelin-1) to 53.5 +/- 1.1% (in the presence of endothelin) and thrombin-stimulated intracellular free calcium concentration from 179 +/- 1.7 nmol/l to 140 +/- 1.8 nmol/l. Calcium 243-250 endothelin 1 Homo sapiens 0-12 7836425-0 1995 Effects of bradykinin and endothelin-1 on the calcium homeostasis of mammalian cells. Calcium 46-53 endothelin 1 Homo sapiens 26-38 7740523-5 1995 A dose and time dependent increase in intracellular calcium by ET-1 occurred in human single neutrophil. Calcium 52-59 endothelin 1 Homo sapiens 63-67 8001384-0 1995 Endothelin-1 increases intracellular calcium in human monocytes and causes production of interleukin-6. Calcium 37-44 endothelin 1 Homo sapiens 0-12 8001384-5 1995 MEASUREMENTS AND MAIN RESULTS: Endothelin-1 increased the intracellular calcium concentration in fura-2 loaded human monocytes to a mean value of 37 +/- 4 nmol. Calcium 72-79 endothelin 1 Homo sapiens 31-43 8587330-7 1995 These observations demonstrate that thrombin stimulates the preproET-1 gene in human endothelial cells through calcium-dependent activation of CaM kinase and protein tyrosine kinases, and that calcineurin may also participate in regulation of the prepro ET-1 gene. Calcium 111-118 endothelin 1 Homo sapiens 66-70 8587393-4 1995 Treatment of single ventricular cells with pertussis toxin (PTX) prevented stimulation of T- and L-type calcium currents by ET-1. Calcium 104-111 endothelin 1 Homo sapiens 124-128 8587448-2 1995 We recently demonstrated that purified furin, a calcium-dependent serine endoprotease belonging to the family of mammalian convertases, cleaved proET-1 in vitro and hence produced the physiologically relevant big ET-1 peptide. Calcium 48-55 endothelin 1 Homo sapiens 147-151 7803503-2 1994 We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. Calcium 116-123 endothelin 1 Homo sapiens 64-68 7705470-6 1994 Therefore, in addition to the activation of mechanisms of [Ca2+]i-myosin phosphorylation, endothelin-1 may activate additional calcium regulatory mechanisms which may thus increase the Ca2+ sensitivity of contractile elements while also enhance and prolong the contractions in the porcine coronary artery. Calcium 127-134 endothelin 1 Homo sapiens 90-102 8001384-9 1995 CONCLUSION: Endothelin-1 increased intracellular calcium in monocytes and caused production of IL-6. Calcium 49-56 endothelin 1 Homo sapiens 12-24 7810625-3 1994 Calcium ionophore A-23187 stimulated basal renin release and potentiated ET-1-stimulated renin release but had no effect on basal or ET-inhibited PRL release. Calcium 0-7 endothelin 1 Homo sapiens 73-77 7888288-5 1994 This study was designed to investigate the effect of glyceryl trinitrate (GTN) and calcium antagonists on ET-1 contraction in the human IMA. Calcium 83-90 endothelin 1 Homo sapiens 106-110 7810625-8 1994 These results indicate that the effects of ET-1 on both decidual renin and PRL release are dependent on the activation of protein kinase C. However, the effect of ET-1 on renin release appears to be dependent on extracellular calcium, whereas the effect on PRL is not influenced by extracellular calcium. Calcium 226-233 endothelin 1 Homo sapiens 163-167 7964729-2 1994 Addition of optimal concentrations of either endothelin-1 (ET-1), ATP, oxotremorine-M (Oxo-M), or norepinephrine (NE) all resulted in an increase in the concentration of cytosolic calcium (Ca2+i) but of different magnitudes (ET-1 = ATP > Oxo-M > NE). Calcium 180-187 endothelin 1 Homo sapiens 45-57 7964729-2 1994 Addition of optimal concentrations of either endothelin-1 (ET-1), ATP, oxotremorine-M (Oxo-M), or norepinephrine (NE) all resulted in an increase in the concentration of cytosolic calcium (Ca2+i) but of different magnitudes (ET-1 = ATP > Oxo-M > NE). Calcium 180-187 endothelin 1 Homo sapiens 59-63 7964729-2 1994 Addition of optimal concentrations of either endothelin-1 (ET-1), ATP, oxotremorine-M (Oxo-M), or norepinephrine (NE) all resulted in an increase in the concentration of cytosolic calcium (Ca2+i) but of different magnitudes (ET-1 = ATP > Oxo-M > NE). Calcium 180-187 endothelin 1 Homo sapiens 225-229 7868873-10 1994 CONCLUSIONS: This study demonstrates that insulin inhibits Ang II- and endothelin-1-stimulated platelet free calcium concentration and pHi, and thrombin-induced aggregation. Calcium 109-116 endothelin 1 Homo sapiens 71-83 8089604-24 1994 Thrombin, LPS, interleukin-8 and ET-1, known calcium agonists could increase intracellular calcium in fibroblasts, amnion and uterine myocytes. Calcium 45-52 endothelin 1 Homo sapiens 33-37 7528845-10 1994 The blocking effect of the calcium antagonist nisoldipine varies among distal and proximal coronary arteries and veins, suggesting a different mechanism of contraction for ET-1 among these vessels. Calcium 27-34 endothelin 1 Homo sapiens 172-176 8089604-24 1994 Thrombin, LPS, interleukin-8 and ET-1, known calcium agonists could increase intracellular calcium in fibroblasts, amnion and uterine myocytes. Calcium 91-98 endothelin 1 Homo sapiens 33-37 8035879-5 1994 Endothelin-1, but not endothelin-3, inhibited the L-type calcium current by decreasing cyclic AMP accumulation and activated the muscarinic potassium current by stimulating a pertussis toxin-sensitive GTP-binding protein. Calcium 57-64 endothelin 1 Homo sapiens 0-12 8389874-3 1993 In subcutaneous arteries preincubation with calcium antagonists partially inhibited responses to endothelin-1. Calcium 44-51 endothelin 1 Homo sapiens 97-109 8267041-10 1993 Substitution of calcium-poor for standard Krebs-Ringers solution in the organ chamber abolished contractions to low endothelin-1 concentrations (< or = 10(-9) mol/L, p < 0.001) but did not affect the contractile response to higher concentrations. Calcium 16-23 endothelin 1 Homo sapiens 116-128 8267041-13 1993 CONCLUSIONS: Endothelin-1 induces contraction of the smooth muscle of human placental veins, which requires the influx of extracellular calcium. Calcium 136-143 endothelin 1 Homo sapiens 13-25 8238579-9 1993 Although calcium entry through dihydropyridine-sensitive Ca2+ channels may account in part for the vasoconstrictor action of endothelin-1 in humans, the abolition of endothelin-1 responses by a K+ channel opener suggests additional mechanisms of action for endothelin-1. Calcium 9-16 endothelin 1 Homo sapiens 125-137 8198607-5 1994 Ten nanomolar ET-1 caused an 8.2 to 25.5-fold increase in intracellular free calcium. Calcium 77-84 endothelin 1 Homo sapiens 14-18 7735949-1 1994 Endothelin-1 (ET-1) is a vasoconstrictive peptide released by ischemic/injured endothelium which increases intracellular ionized calcium [Ca2+]i in vascular smooth muscle. Calcium 129-136 endothelin 1 Homo sapiens 0-12 7735949-1 1994 Endothelin-1 (ET-1) is a vasoconstrictive peptide released by ischemic/injured endothelium which increases intracellular ionized calcium [Ca2+]i in vascular smooth muscle. Calcium 129-136 endothelin 1 Homo sapiens 14-18 8294922-9 1994 ET-1 also stimulated endothelial prostaglandin F2 alpha production, suggesting that activation of phospholipase A2 is most likely secondary to IP3-mediated intracellular calcium mobilization because both ET-1-induced IP3 and prostaglandin F2 alpha were inhibited by BQ123. Calcium 170-177 endothelin 1 Homo sapiens 0-4 8294922-9 1994 ET-1 also stimulated endothelial prostaglandin F2 alpha production, suggesting that activation of phospholipase A2 is most likely secondary to IP3-mediated intracellular calcium mobilization because both ET-1-induced IP3 and prostaglandin F2 alpha were inhibited by BQ123. Calcium 170-177 endothelin 1 Homo sapiens 204-208 8124662-1 1994 Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line. Calcium 103-110 endothelin 1 Homo sapiens 46-58 8124662-1 1994 Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line. Calcium 103-110 endothelin 1 Homo sapiens 60-64 8156652-5 1994 The results showed that BQ123 and the calcium antagonists nisoldipine, isradipine, nitrendipine and nifedipine fully relaxed IMA precontracted with 3 nmol/L ET-1 with the EC50 values of 7.18 +/- 0.09 (-log mol/L) for BQ123, and 7.68 +/- 0.07, 7.02 +/- 0.12, 6.96 +/- 0.08 and 6.89 +/- 0.09 for the calcium antagonists, respectively. Calcium 38-45 endothelin 1 Homo sapiens 157-161 8156652-5 1994 The results showed that BQ123 and the calcium antagonists nisoldipine, isradipine, nitrendipine and nifedipine fully relaxed IMA precontracted with 3 nmol/L ET-1 with the EC50 values of 7.18 +/- 0.09 (-log mol/L) for BQ123, and 7.68 +/- 0.07, 7.02 +/- 0.12, 6.96 +/- 0.08 and 6.89 +/- 0.09 for the calcium antagonists, respectively. Calcium 298-305 endothelin 1 Homo sapiens 157-161 8207562-7 1994 If this can be confirmed, calcium antagonists, which are known to antagonize endothelin-1 mediated vasoconstriction, may be used to alleviate the pulmonary hypertension that occasionally occurs under these specific circumstances. Calcium 26-33 endothelin 1 Homo sapiens 77-89 8100861-12 1993 A calcium-free buffer abolished the contractile response to ET1. Calcium 2-9 endothelin 1 Homo sapiens 60-63 8389874-6 1993 The role of influx of extracellular calcium through voltage operated calcium channels in endothelin-1-induced contraction appear to differ between subcutaneous and omental resistance arteries. Calcium 36-43 endothelin 1 Homo sapiens 89-101 8488329-3 1993 ET-1 action involves phosphatidylinositol turnover, calcium mobilization and protein kinase C activation. Calcium 52-59 endothelin 1 Homo sapiens 0-4 8447814-6 1993 These findings indicate that endothelins can induce an increase of HBEC permeability by a receptor-specific activation of PKC and intracellular calcium mobilization. Calcium 144-151 endothelin 1 Homo sapiens 29-40 1334843-6 1992 Furthermore, an endothelin-1-induced increase in intracellular calcium level was observed in MEG-01 cells, strongly suggesting the expression of endothelin receptors on platelet precursors cells, whereas the presence of such receptors is controversial on platelets. Calcium 63-70 endothelin 1 Homo sapiens 16-28 8098849-2 1993 Endothelin-1 and ET-2 induced cytosolic free calcium elevations that did not return to the basal level. Calcium 45-52 endothelin 1 Homo sapiens 0-12 7509995-0 1993 Calcium ionophores inhibit the release of endothelin-1 from endothelial cells. Calcium 0-7 endothelin 1 Homo sapiens 42-54 1280264-9 1992 In a parallel study, the medium conditioned by UVB-exposed human keratinocytes elicited a significant anti-ET-1 antibody-suppressible increase in DNA synthesis by cultured human melanocytes in a UV dose-dependent manner, which was associated with a marked and rapid (80 s) increase in the intracellular calcium level upon incubation with human melanocytes. Calcium 303-310 endothelin 1 Homo sapiens 107-111 1644929-3 1992 In the present study we have analyzed whether FSC respond to local vasoconstrictors such as thrombin, angiotensin-II, and endothelin-1 with an increase in intracellular free calcium concentration ([Ca2+]i) coupled with effective cell contraction. Calcium 174-181 endothelin 1 Homo sapiens 122-134 1407005-0 1992 Calcium mobilization and its desensitization induced by endothelins and sarafotoxin in human astrocytoma cells (1321N1): comparison of histamine-induced calcium mobilization. Calcium 0-7 endothelin 1 Homo sapiens 56-67 1425954-3 1992 Endothelin-1, -2 and -3 had a dual action, on platelet aggregation and calcium mobilization induced by serotonin. Calcium 71-78 endothelin 1 Homo sapiens 0-23 1639963-0 1992 The regulation of endothelin production in human umbilical vein endothelial cells: unique inhibitory action of calcium ionophores. Calcium 111-118 endothelin 1 Homo sapiens 18-28 1794204-10 1991 Furthermore, the calcium-mobilizing agonists markedly stimulated endothelin-1 release by activating endothelin-1 gene transcription. Calcium 17-24 endothelin 1 Homo sapiens 65-77 1377954-8 1992 The cytosolic calcium accumulation was significantly increased by endothelin-1. Calcium 14-21 endothelin 1 Homo sapiens 66-78 1377954-9 1992 These results suggest that endothelin-1 decreases the release of t-PA:Ag from human endothelial cells through an excess accumulation of intracellular, especially cytosolic which would be mediated by an extracellular, calcium-dependent mechanism. Calcium 217-224 endothelin 1 Homo sapiens 27-39 1794204-10 1991 Furthermore, the calcium-mobilizing agonists markedly stimulated endothelin-1 release by activating endothelin-1 gene transcription. Calcium 17-24 endothelin 1 Homo sapiens 100-112 1794204-11 1991 Glomerular mesangial cells respond to EDRF with a rise in cytosolic cGMP concentration and relaxation, and to endothelin-1 with a rise in cytosolic calcium concentration and contraction. Calcium 148-155 endothelin 1 Homo sapiens 110-122 1657061-0 1991 Mechanisms of calcium mobilization and phosphoinositide hydrolysis in human bronchial smooth muscle cells by endothelin 1. Calcium 14-21 endothelin 1 Homo sapiens 109-121 1656037-0 1991 Calcium dependent and independent endothelin-1-induced contraction of human omentum vessels. Calcium 0-7 endothelin 1 Homo sapiens 34-46 1917001-8 1991 Although the mechanisms leading to vasodilation are not clear in humans, endothelin-1-induced vasoconstriction appears to be completely dependent on the activity of voltage-operated calcium channels and can be blocked by organic calcium antagonists but not by nitrovasodilators or EDRF. Calcium 182-189 endothelin 1 Homo sapiens 73-85 1917960-4 1991 Upon incubation with cultured cells, the mass contents of inositol 1,4,5-trisphosphate and intracellular calcium level were substantially increased by 10 nM ET-1, ET-2, and ET-3, but not by big-ET with maximal response at 80-130-s postincubation. Calcium 105-112 endothelin 1 Homo sapiens 157-161 1656037-3 1991 However, when calcium depletion was combined with pretreatment with the protein kinase C inhibitor (H7), endothelin-1 failed to produce a contraction. Calcium 14-21 endothelin 1 Homo sapiens 105-117 2119298-10 1990 Perifusion of adrenal slices with a calcium-free solution or addition of cobalt (4 mM) induced total inhibition of the stimulatory effect of ET-1. Calcium 36-43 endothelin 1 Homo sapiens 141-145 1846783-6 1991 In contrast, both calcium antagonists converted ET-induced vasoconstriction (e.g., delta forearm vascular resistance to ET 50 ng/min/100 ml forearm tissue, 151 +/- 100% and 164 +/- 92% in verapamil and nifedipine groups, respectively) to vasodilation (-35 +/- 12% and -21 +/- 16%, p less than 0.05). Calcium 18-25 endothelin 1 Homo sapiens 48-50 1846783-6 1991 In contrast, both calcium antagonists converted ET-induced vasoconstriction (e.g., delta forearm vascular resistance to ET 50 ng/min/100 ml forearm tissue, 151 +/- 100% and 164 +/- 92% in verapamil and nifedipine groups, respectively) to vasodilation (-35 +/- 12% and -21 +/- 16%, p less than 0.05). Calcium 18-25 endothelin 1 Homo sapiens 120-122 1849534-4 1991 Calcium influx, phospholipase C and ACE activity were suppressed 60-70% in the presence of endothelin-1 (10(-10) to 10(-6) mol/l) by 50 microliters neomycin. Calcium 0-7 endothelin 1 Homo sapiens 91-103 2028454-1 1991 Endothelin-1, a peptide produced by endothelium, causes vascular smooth muscle contraction possibly by mobilizing intracellular calcium. Calcium 128-135 endothelin 1 Homo sapiens 0-12 1849534-3 1991 Endothelin-1 also stimulated calcium influx and phospholipase C activity in a dose-dependent manner. Calcium 29-36 endothelin 1 Homo sapiens 0-12 2119298-13 1990 The data also indicate that ET-1-evoked corticosteroid secretion is mediated by an increase in PG biosynthesis and requires the presence of extracellular calcium. Calcium 154-161 endothelin 1 Homo sapiens 28-32 2172374-0 1990 Effect of endothelin-1 on cytosolic calcium ions in cultured human endothelial cells. Calcium 36-43 endothelin 1 Homo sapiens 10-22 1706809-0 1990 Different mobilization of calcium in endothelin-1-induced contractions in human arteries and veins: effects of calcium antagonists. Calcium 26-33 endothelin 1 Homo sapiens 37-49 2698922-5 1989 These experiments suggest that an endothelin-1-induced contraction is largely dependent on the presence of extracellular calcium, but a partial contraction can be induced in a calcium-free medium and this may be dependent on mobilization of intracellular calcium from a store not affected by repeated noradrenaline stimulation. Calcium 121-128 endothelin 1 Homo sapiens 34-46 2698922-5 1989 These experiments suggest that an endothelin-1-induced contraction is largely dependent on the presence of extracellular calcium, but a partial contraction can be induced in a calcium-free medium and this may be dependent on mobilization of intracellular calcium from a store not affected by repeated noradrenaline stimulation. Calcium 176-183 endothelin 1 Homo sapiens 34-46 2698922-5 1989 These experiments suggest that an endothelin-1-induced contraction is largely dependent on the presence of extracellular calcium, but a partial contraction can be induced in a calcium-free medium and this may be dependent on mobilization of intracellular calcium from a store not affected by repeated noradrenaline stimulation. Calcium 176-183 endothelin 1 Homo sapiens 34-46 25684033-6 2015 Effects of lidocaine and bupivacaine, along with their respective membrane-impermeant derivatives QX-314, LEA-123 and LEA-124, on peak calcium responses to ET-1 were measured. Calcium 135-142 endothelin 1 Homo sapiens 156-160 31606530-6 2019 Furthermore, we found that substrate stiffening reduced the expression and activity of the calcium channel TRPV4, which subsequently enhanced ET-1 expression by inhibiting miR-6740-5p. Calcium 91-98 endothelin 1 Homo sapiens 142-146 29693418-6 2018 However, quercetin inhibited KCl induced extracellular calcium influx and ET-1 induced transient intracellular calcium release in a Ca2+-free solution. Calcium 111-118 endothelin 1 Homo sapiens 74-78 28059438-9 2017 Population doubling time for the smooth muscle cells was ~70 hr at passages 3 and 7; and the cells positively responded to endothelin-1, as shown by a 66% increase in the intracellular calcium level. Calcium 185-192 endothelin 1 Homo sapiens 123-135 28205210-0 2017 Urinary sodium and calcium excretion: via endothelin-1 do they part? Calcium 19-26 endothelin 1 Homo sapiens 42-54 27761593-4 2017 We found that activation of CREB-dependent transcription reduced the calcium responses induced by ATP, noradrenaline, or endothelin-1. Calcium 69-76 endothelin 1 Homo sapiens 121-133 25684033-0 2015 Bupivacaine inhibits endothelin-1-evoked increases in intracellular calcium in model sensory neurons. Calcium 68-75 endothelin 1 Homo sapiens 21-33 26164720-0 2015 Nuclear Membranes ETB Receptors Mediate ET-1-induced Increase of Nuclear Calcium in Human Left Ventricular Endocardial Endothelial Cells. Calcium 73-80 endothelin 1 Homo sapiens 40-44 26164720-1 2015 In fetal human left ventricular endocardial endothelial cells (EECLs), both plasma membrane (PM) ET(A)R and ET(B)R were reported to mediate ET-1-induced increase of intracellular calcium [Ca](i); however, this effect was mediated by ET(A)R in right EECs (EECRs). Calcium 179-186 endothelin 1 Homo sapiens 140-144 26164720-2 2015 In this study, we verified whether, as for the PM, nuclear membranes (NMs) ET-1 receptors activation in EECLs and EECRs induce an increase of nuclear calcium ([Ca](n)) and if this effect is mediated through the same receptor type as in PM. Calcium 150-157 endothelin 1 Homo sapiens 75-79 25873305-0 2015 Divergence in endothelin-1- and bradykinin-activated store-operated calcium entry in afferent sensory neurons. Calcium 68-75 endothelin 1 Homo sapiens 14-26 20532602-7 2010 Endothelin-1, acting via two G-protein-coupled receptors (ETA and ETB), prolongs the action potential duration and increases the occurrence of spontaneous calcium transients, resulting in early afterdepolarizations and ventricular tachyarrhythmias via triggered activity. Calcium 155-162 endothelin 1 Homo sapiens 0-12 25741585-0 2015 Nuclear membrane R-type calcium channels mediate cytosolic ET-1-induced increase of nuclear calcium in human vascular smooth muscle cells. Calcium 24-31 endothelin 1 Homo sapiens 59-63 25741585-1 2015 The objective of this work was to verify whether, as in the case of the plasma membrane of human vascular smooth muscle cells (hVSMCs), cytosolic ET-1-induced increase of nuclear calcium is mediated via the activation of calcium influx through the steady-state R-type calcium channel. Calcium 179-186 endothelin 1 Homo sapiens 146-150 25741585-1 2015 The objective of this work was to verify whether, as in the case of the plasma membrane of human vascular smooth muscle cells (hVSMCs), cytosolic ET-1-induced increase of nuclear calcium is mediated via the activation of calcium influx through the steady-state R-type calcium channel. Calcium 221-228 endothelin 1 Homo sapiens 146-150 25741585-3 2015 Our results showed that pre-treatment with pertussis toxin (PTX) or cholera toxin (CTX) prevented cytosolic ET-1 (10(-9) mol/L) from inducing a sustained increase in nuclear calcium. Calcium 174-181 endothelin 1 Homo sapiens 108-112 25607638-5 2015 The effects of endothelin-1 were dependent on increasing intracellular calcium mobilization by endothelin B receptor signalling. Calcium 71-78 endothelin 1 Homo sapiens 15-27 25140913-9 2015 Thus, endothelin-1 and angiotensin II induce afferent arteriolar constriction via LC20 phosphorylation at Ser19 due to calcium activation of MLCK and ROCK-mediated inhibition of MLCP. Calcium 119-126 endothelin 1 Homo sapiens 6-18 23344160-4 2013 Specifically, 1,25-dihydroxyvitamin D(3) (24-h pretreatment) caused a more than threefold enhancement in both ET-1-induced intracellular calcium mobilization and extracellular signal-regulated kinase (ERK) activation. Calcium 137-144 endothelin 1 Homo sapiens 110-114 23086942-4 2012 We provide evidence that microinjected endothelin-1 produces a dose-dependent elevation in cytosolic calcium concentration in ET(B)-transfected cells and endothelial cells; this response is sensitive to ET(B) but not ET(A) receptor blockade. Calcium 101-108 endothelin 1 Homo sapiens 39-51 25741585-5 2015 In conclusion, the preventative effect with PTX and CTX, and the absence of an effect with nifedipine, as well as the blockade by isradipine on cytosolic ET-1-induced increase in nuclear calcium, suggest that this nuclear calcium influx in hVSMCs is due to activation of the steady-state R-type calcium channel. Calcium 187-194 endothelin 1 Homo sapiens 154-158 25741585-5 2015 In conclusion, the preventative effect with PTX and CTX, and the absence of an effect with nifedipine, as well as the blockade by isradipine on cytosolic ET-1-induced increase in nuclear calcium, suggest that this nuclear calcium influx in hVSMCs is due to activation of the steady-state R-type calcium channel. Calcium 222-229 endothelin 1 Homo sapiens 154-158 23434582-4 2013 Our results indicated that endothelin-1 decreased the protein level of NaV1.7 and the veratridine-induced increase in intracellular calcium. Calcium 132-139 endothelin 1 Homo sapiens 27-39 23221682-8 2013 Rendomab-B1 not only blocks ET-1-induced calcium signaling pathway and triggers rapid receptor internalization on recombinant hETBR-expressing cells, but also exerts pharmacological activities on human vascular endothelial cells, reducing both cell viability and ET-1-induced hETBR synthesis. Calcium 41-48 endothelin 1 Homo sapiens 28-32 21765001-6 2011 An hour after a simulated blast, injured tissues displayed altered intracellular calcium dynamics leading to hypersensitivity to contractile stimulus with endothelin-1. Calcium 81-88 endothelin 1 Homo sapiens 155-167 20452970-11 2010 ET-1 increases in intracellular calcium and gene transcription were dependent upon ET(a) activation and calcium influx through T type voltage-dependent calcium channels. Calcium 32-39 endothelin 1 Homo sapiens 0-4 20452970-11 2010 ET-1 increases in intracellular calcium and gene transcription were dependent upon ET(a) activation and calcium influx through T type voltage-dependent calcium channels. Calcium 104-111 endothelin 1 Homo sapiens 0-4 20725140-4 2010 Using intact and isolated human hVECs and the Fura-2 calcium (Ca2+) measurement technique, we showed that ET-1 induced a dose-dependent increase of total intracellular free Ca2+, with an EC50 of 1.3 x 10-10 mol/L. Calcium 53-60 endothelin 1 Homo sapiens 106-110 20662769-2 2010 Most of the effects of ET-1 are associated with an increase in intracellular calcium concentration. Calcium 77-84 endothelin 1 Homo sapiens 23-27 20662769-3 2010 The calcium influx and mobilization pathways activated by ET-1, however, vary immensely. Calcium 4-11 endothelin 1 Homo sapiens 58-62 20662769-6 2010 We also examine how disease alters calcium-dependent responses to ET-1 by discussing changes to ET-1-mediated calcium signalling in hypertension, as there is significant interest in the role of ET-1 in this important disease. Calcium 35-42 endothelin 1 Homo sapiens 66-70 20662769-6 2010 We also examine how disease alters calcium-dependent responses to ET-1 by discussing changes to ET-1-mediated calcium signalling in hypertension, as there is significant interest in the role of ET-1 in this important disease. Calcium 110-117 endothelin 1 Homo sapiens 66-70 20662769-6 2010 We also examine how disease alters calcium-dependent responses to ET-1 by discussing changes to ET-1-mediated calcium signalling in hypertension, as there is significant interest in the role of ET-1 in this important disease. Calcium 110-117 endothelin 1 Homo sapiens 96-100 20662769-6 2010 We also examine how disease alters calcium-dependent responses to ET-1 by discussing changes to ET-1-mediated calcium signalling in hypertension, as there is significant interest in the role of ET-1 in this important disease. Calcium 110-117 endothelin 1 Homo sapiens 96-100 20662769-7 2010 A list of unanswered questions regarding ET-mediated calcium signals are also presented, as well as perspectives for future research of calcium mobilization by ET-1. Calcium 136-143 endothelin 1 Homo sapiens 160-164 18424490-0 2008 Endothelin receptor dimers evaluated by FRET, ligand binding, and calcium mobilization. Calcium 66-73 endothelin 1 Homo sapiens 0-10 18436224-5 2009 Furthermore, ET-1 increased intracellular O(2*)(-) production in all layers of the vessel, protein expression of NADPH oxidase subunit p47(phox) without affecting p22(phox) expression and lucigenin-enhanced chemiluminescence signal stimulated by calcium ionophore A23187. Calcium 246-253 endothelin 1 Homo sapiens 13-17 19338156-4 2009 ET-1 and SA-B were directly added to the serum-free medium of HSCs, then calcium ion concentration was detected by laser scanning confocal microscope. Calcium 73-80 endothelin 1 Homo sapiens 0-4 19338156-9 2009 Laser scanning confocal microscope experiments revealed that ET-1 stimulated the transiently rapid increase of intracellular calcium ion concentration, and the effects was obviously inhibited when SA-B was added. Calcium 125-132 endothelin 1 Homo sapiens 61-65 20735957-0 2008 [The effect and mechanism of endothelin-1-induced intracellular free calcium in human lung adenocarcinoma cells SPC-A1.]. Calcium 69-76 endothelin 1 Homo sapiens 29-41 20452970-0 2010 Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop. Calcium 106-113 endothelin 1 Homo sapiens 0-12 20452970-0 2010 Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop. Calcium 106-113 endothelin 1 Homo sapiens 14-18 21547002-8 2009 In vascular smooth muscle, vasoconstrictors such as angiotensin II, endothelin-1 and norepinephrine stimulate contraction by increasing inositol trisphosphate (IP(3)), calcium, DG and PKC activity. Calcium 168-175 endothelin 1 Homo sapiens 68-80 17433294-5 2007 A dose-dependent increase in intra-cellular calcium concentrations was observed in the presence of 1, 10 and 100nM ET-1. Calcium 44-51 endothelin 1 Homo sapiens 115-119 18257749-7 2008 4 Cell pretreatment with PP2 amplified the increase in intracellular-free calcium observed with NA, in the basal state and after the stimulation (desensitization) induced by ET-1. Calcium 74-81 endothelin 1 Homo sapiens 174-178 17433294-7 2007 Desensitization to ET(A)-mediated increase in intracellular calcium was observed in LC cells following pre-treatment with ET-1 for 24h. Calcium 60-67 endothelin 1 Homo sapiens 122-126 17027026-0 2006 How does endothelin-1 cause a sustained increase in intracellular sodium and calcium which lead to hypertrophy? Calcium 77-84 endothelin 1 Homo sapiens 9-21 17178974-3 2007 We tested whether calcium antagonists also influence the biological activity of ET-1 in essential hypertensive (EH) patients in the presence and absence of hypercholesterolemia. Calcium 18-25 endothelin 1 Homo sapiens 80-84 16878432-2 2006 ET-1 displays an in vitro inhibitory effect on basal parathyroid hormone (PTH) secretion and also counteracts PTH hypersecretion stimulated by low calcium. Calcium 147-154 endothelin 1 Homo sapiens 0-4 16878432-13 2006 RESULTS: ET-1 exhibited a dose-dependent inhibitory effect (from 10(-12) - 10(-7) M) on PTH mRNA expression of parathyroid cells, either in the basal or in the low-calcium-stimulated states. Calcium 164-171 endothelin 1 Homo sapiens 9-13 16877529-16 2006 Pretreatment of human VSMCs from internal mammary arteries with CRM197 significantly blunted ET-1-stimulated calcium transients. Calcium 109-116 endothelin 1 Homo sapiens 93-97