PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9821863-1 1998 The ability of dihydrosphingosine to release Ca2+ from intracellular stores in neurones was investigated by combining the whole cell patch clamp technique with intracellular flash photolysis of caged, N-(2-nitrobenzyl)dihydrosphingosine. safingol 15-33 carbonic anhydrase 2 Rattus norvegicus 45-48 9821863-3 1998 Cultured dorsal root ganglion neurones from neonatal rats were voltage clamped at -90 mV and inward whole cell Ca2+-activated currents were recorded in response to intracellular photorelease of dihydrosphingosine. safingol 194-212 carbonic anhydrase 2 Rattus norvegicus 111-114 9821863-9 1998 The responses to photoreleased dihydrosphingosine were inhibited by intracellular application of 20 mM EGTA, 10 microM ryanodine or extracellular application of 10 microM dantrolene, but persisted when Ca2+ free saline was applied to the extracellular environment. safingol 31-49 carbonic anhydrase 2 Rattus norvegicus 202-205 9821863-10 1998 Intracellular application of uncaged dihydrosphingosine evoked responses which were attenuated by photolysis of the caged Ca2+ chelator Diazo-2. safingol 37-55 carbonic anhydrase 2 Rattus norvegicus 122-125 9821863-12 1998 We conclude that dihydrosphingosine directly or indirectly mobilises Ca2+ from ryanodine-sensitive intracellular stores in cultured sensory neurones. safingol 17-35 carbonic anhydrase 2 Rattus norvegicus 69-72