PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24686197-1 2014 Homotrimeric mammalian purine nucleoside phosphorylase (PNP) plays a key role in the nucleoside and nucleotide metabolic salvage pathway. Nucleosides 30-40 purine nucleoside phosphorylase Homo sapiens 56-59 29119917-3 2017 METHODS: Unlike human purine nucleoside phosphorylase (PNP), E. coli PNP accepts adenine containing nucleosides as substrates, and is therefore able to selectively activate non-toxic purine analogs in tumor tissue. Nucleosides 100-111 purine nucleoside phosphorylase Homo sapiens 69-72 20525731-1 2010 Purine nucleoside phosphorylase (PNP) catalyzes the synthesis and phosphorolysis of purine nucleosides, interconverting nucleosides with their corresponding purine base and ribose-1-phosphate. Nucleosides 91-102 purine nucleoside phosphorylase Homo sapiens 0-31 24140360-1 2014 Two nontypical nucleosides, 7-beta-D-ribosyl-2,6-diamino-8-azapurine and 8-beta-D-ribosyl-2,6-diamino-8-azapurine, have been found to exhibit moderately good, and selective, substrate properties toward calf and bacterial (Escherichia coli) forms of purine nucleoside phosphorylase (PNP). Nucleosides 15-26 purine nucleoside phosphorylase Homo sapiens 249-280 23621450-1 2013 Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides. Nucleosides 146-157 purine nucleoside phosphorylase Homo sapiens 0-31 22629603-2 2011 PNP plays a leading role in the cell metabolism of nucleosides and nucleotides, as well as in maintaining the immune status of an organism. Nucleosides 51-62 purine nucleoside phosphorylase Homo sapiens 0-3 20525731-1 2010 Purine nucleoside phosphorylase (PNP) catalyzes the synthesis and phosphorolysis of purine nucleosides, interconverting nucleosides with their corresponding purine base and ribose-1-phosphate. Nucleosides 91-102 purine nucleoside phosphorylase Homo sapiens 33-36 20525731-1 2010 Purine nucleoside phosphorylase (PNP) catalyzes the synthesis and phosphorolysis of purine nucleosides, interconverting nucleosides with their corresponding purine base and ribose-1-phosphate. Nucleosides 120-131 purine nucleoside phosphorylase Homo sapiens 0-31 20525731-1 2010 Purine nucleoside phosphorylase (PNP) catalyzes the synthesis and phosphorolysis of purine nucleosides, interconverting nucleosides with their corresponding purine base and ribose-1-phosphate. Nucleosides 120-131 purine nucleoside phosphorylase Homo sapiens 33-36 20525731-2 2010 While PNP plays significant roles in human and pathogen physiology, we are interested in developing PNP as a catalyst for the formation of nucleoside analog drugs of clinical relevance. Nucleosides 139-149 purine nucleoside phosphorylase Homo sapiens 100-103 16679527-5 2006 Purine nucleoside phosphorylase catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 96-107 purine nucleoside phosphorylase Homo sapiens 0-31 15983407-1 2005 Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. Nucleosides 135-146 purine nucleoside phosphorylase Homo sapiens 0-31 15983407-1 2005 Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. Nucleosides 135-146 purine nucleoside phosphorylase Homo sapiens 33-36 12509998-1 2003 Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 102-113 purine nucleoside phosphorylase Homo sapiens 0-31 15812229-1 2005 The E. coli PNP suicide gene sensitizes solid tumors to nucleoside prodrugs, such as 6-methylpurine-2"-deoxyriboside (MeP-dR). Nucleosides 56-66 purine nucleoside phosphorylase Homo sapiens 12-15 15448350-8 2004 From HPLC analysis, the recombinant GIV PNP protein was shown to catalyse the reversible phosphorolysis of purine nucleosides and could accept guanosine, inosine and adenosine as substrates. Nucleosides 114-125 purine nucleoside phosphorylase Homo sapiens 40-43 14680831-1 2003 Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 102-113 purine nucleoside phosphorylase Homo sapiens 0-31 14680831-1 2003 Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 102-113 purine nucleoside phosphorylase Homo sapiens 33-36 12914785-1 2003 Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 102-113 purine nucleoside phosphorylase Homo sapiens 0-31 12914785-1 2003 Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 102-113 purine nucleoside phosphorylase Homo sapiens 33-36 12730608-5 2003 First, it was determined if compounds that influenced AsV reduction by purified PNP (i.e., nucleosides, thiols, and PNP inhibitors) would similarly affect reduction of AsV by human erythrocytes. Nucleosides 91-102 purine nucleoside phosphorylase Homo sapiens 80-83 12509998-1 2003 Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. Nucleosides 102-113 purine nucleoside phosphorylase Homo sapiens 33-36 12173924-6 2002 Steady-state kinetic analysis of T. vaginalis PNP-catalyzed reactions gave K(m)"s of 31.5, 59.7, and 6.1 microM for inosine, guanosine, and adenosine in the nucleosidase reaction and 45.6, 35.9, and 12.3 microM for hypoxanthine, guanine, and adenine in the direction of nucleoside synthesis. Nucleosides 270-280 purine nucleoside phosphorylase Homo sapiens 46-49 3261757-2 1988 In the presence of coformycin, an adenosine deaminase inhibitor, or formycin B, a purine nucleoside phosphorylase inhibitor, DNA cleavage was induced by these nucleosides at concentrations of less than 50 microM. Nucleosides 159-170 purine nucleoside phosphorylase Homo sapiens 82-113 11337031-8 2000 Also described are applications of PNP from various sources as tools for the enzymatic synthesis of otherwise inaccessible therapeutic nucleoside analogues, as coupling enzymes for assays of orthophosphate in biological systems in the micromolar and submicromolar ranges, and for coupled assays of other enzyme systems. Nucleosides 135-145 purine nucleoside phosphorylase Homo sapiens 35-38 34942489-1 2022 Human purine nucleoside phosphorylase (HsPNP) catalyzes reversible phosphorolysis of nucleosides and deoxynucleosides in the purine cascade. Nucleosides 85-96 purine nucleoside phosphorylase Homo sapiens 6-37 105760-3 1979 Nucleoside analog inhibition studies have been conducted on thyroidal purine nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase, EC 2.4.2.1) which catalyzed an ordered bi-bi type mechanism where the first substrate is inorganic phosphate and the last product is ribose 1-phosphate. Nucleosides 0-10 purine nucleoside phosphorylase Homo sapiens 103-154 6332827-7 1984 An adenosine deaminase-deficient and a purine nucleoside phosphorylase-deficient cell line, both of B cell origin, exhibited sensitivities to the nucleosides similar to those of the normal B cell lines. Nucleosides 146-157 purine nucleoside phosphorylase Homo sapiens 39-70 6420188-3 1984 Only nucleosides were excreted during GTP catabolism by PNP deficient cells and the main product was guanosine. Nucleosides 5-16 purine nucleoside phosphorylase Homo sapiens 56-59 6789872-4 1981 The other enzyme, purine nucleoside phosphorylase (PN Pase), catalyzed the synthesis of the product nucleoside by utilizing the pentose 1-phosphate ester generated from the phosphorolysis of the pyrimidine nucleoside. Nucleosides 25-35 purine nucleoside phosphorylase Homo sapiens 51-58 206294-5 1978 For the inhibitory activity of pNp to occur, the intactness of the nucleoside moiety as a whole was shown to be necessary, the activity level depending on the structure of both the base and the sugar components. Nucleosides 67-77 purine nucleoside phosphorylase Homo sapiens 31-34 115663-2 1978 After each transfusion with PNP-containing erythrocytes a decrease in accumulated nucleosides and their deoxy compounds was observed, whereas uric acid excretion and serum uric acid increased. Nucleosides 82-93 purine nucleoside phosphorylase Homo sapiens 28-31 33190694-0 2020 Non-typical nucleoside analogs as fluorescent and fluorogenic indicators of purine-nucleoside phosphorylase activity in biological samples. Nucleosides 12-22 purine nucleoside phosphorylase Homo sapiens 76-107 33715357-0 2021 Symmetric Nucleosides as Potent Purine Nucleoside Phosphorylase Inhibitors. Nucleosides 10-21 purine nucleoside phosphorylase Homo sapiens 32-63 33715357-4 2021 Here, we study the binding of human purine nucleoside phosphorylase (PNP) to some bidirectional symmetric nucleosides, a class of nucleoside analogues that are more flexible due to the absence of sugar pucker restraints. Nucleosides 106-117 purine nucleoside phosphorylase Homo sapiens 36-67 33715357-4 2021 Here, we study the binding of human purine nucleoside phosphorylase (PNP) to some bidirectional symmetric nucleosides, a class of nucleoside analogues that are more flexible due to the absence of sugar pucker restraints. Nucleosides 106-117 purine nucleoside phosphorylase Homo sapiens 69-72 33715357-4 2021 Here, we study the binding of human purine nucleoside phosphorylase (PNP) to some bidirectional symmetric nucleosides, a class of nucleoside analogues that are more flexible due to the absence of sugar pucker restraints. Nucleosides 43-53 purine nucleoside phosphorylase Homo sapiens 69-72 33715357-5 2021 We compared the binding energies of PNP-symmetric nucleosides to the binding energies of PNP-inosine/Imm-H (a transition-state analogue), by means of 200 ns long all-atom explicit-solvent Gaussian accelerated molecular dynamics simulations followed by energetics estimation using the MM-PBSA methodology. Nucleosides 50-61 purine nucleoside phosphorylase Homo sapiens 36-39 33715357-6 2021 Quite interestingly, we observed that a few symmetric nucleosides, namely, nu3 and nu4, showed strong binding with PNP (-14.1 and -12.6 kcal/mol, respectively), higher than inosine (-6.3 kcal/mol) and Imm-H (-9.6 kcal/mol). Nucleosides 54-65 purine nucleoside phosphorylase Homo sapiens 115-118