PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20360301-0 2010 Vectorial transport of nucleoside analogs from the apical to the basolateral membrane in double-transfected cells expressing the human concentrative nucleoside transporter hCNT3 and the export pump ABCC4. Nucleosides 23-33 solute carrier family 28 member 3 Homo sapiens 172-177 26102284-11 2015 Darunavir strongly induced expression in most cell lines of CNT3 involved in cell uptake of nucleotide/nucleoside analogue reverse transcriptase inhibitors and SLCO drug transporters involved in cell uptake of protease inhibitors. Nucleosides 103-113 solute carrier family 28 member 3 Homo sapiens 60-64 25713533-4 2015 They translocate nucleosides in a Na(+) coupled manner with high affinity and some substrate selectivity, being hCNT1 and hCNT2 pyrimidine- and purine-preferring, respectively, and hCNT3 a broad selectivity transporter. Nucleosides 17-28 solute carrier family 28 member 3 Homo sapiens 181-186 22492015-1 2012 SLC28 genes encode three plasma membrane transporter proteins, human concentrative nucleoside transporter (CNT)1, CNT2, and CNT3, all of which are implicated in the uptake of natural nucleosides and a variety of nucleoside analogs used in the chemotherapy of cancer and viral and inflammatory diseases. Nucleosides 183-194 solute carrier family 28 member 3 Homo sapiens 124-128 22492015-1 2012 SLC28 genes encode three plasma membrane transporter proteins, human concentrative nucleoside transporter (CNT)1, CNT2, and CNT3, all of which are implicated in the uptake of natural nucleosides and a variety of nucleoside analogs used in the chemotherapy of cancer and viral and inflammatory diseases. Nucleosides 83-93 solute carrier family 28 member 3 Homo sapiens 124-128 23441192-3 2013 We tested whether transfection of human concentrative nucleoside transporter 3 (hCNT3) using ultrasound and lipid stabilized microbubbles could increase gemcitabine uptake and sensitivity in HEK293 cells made nucleoside transport deficient by pharmacologic treatment with dilazep. Nucleosides 54-64 solute carrier family 28 member 3 Homo sapiens 80-85 20643903-1 2010 Human concentrative nucleoside transporter 3 (hCNT3) is a broad-selectivity, high-affinity protein implicated in the uptake of most nucleoside-derived anticancer and antiviral drugs. Nucleosides 20-30 solute carrier family 28 member 3 Homo sapiens 46-51 20643903-2 2010 Regulated trafficking of hCNT3 has been recently postulated as a suitable way to improve nucleoside-based therapies. Nucleosides 89-99 solute carrier family 28 member 3 Homo sapiens 25-30 20421346-1 2010 The human concentrative nucleoside transporter-3 C602R (hCNT3C602R), a recently identified human concentrative nucleoside transporter-3 (hCNT3) variant, has been shown to interact with natural nucleosides with apparent K(m) values similar to those of the wild-type transporter, although binding of one of the two sodium ions required for nucleoside translocation is impaired, resulting in decreased V(max) values (Mol Pharmacol 73:379-386, 2008). Nucleosides 193-204 solute carrier family 28 member 3 Homo sapiens 56-61 20421346-2 2010 We have further analyzed the properties of this hCNT3 variant by determining its localization in plasma membrane lipid domains and its interaction with nucleoside-derived drugs used in anticancer and antiviral therapies. Nucleosides 152-162 solute carrier family 28 member 3 Homo sapiens 48-53 20495821-1 2010 Human concentrative nucleoside transporter 3 (hCNT3) uses the electrochemical gradient of Na(+) and H(+) to drive the transport of nucleosides and therapeutic nucleoside analogs into the cells. Nucleosides 131-142 solute carrier family 28 member 3 Homo sapiens 46-51 20495821-1 2010 Human concentrative nucleoside transporter 3 (hCNT3) uses the electrochemical gradient of Na(+) and H(+) to drive the transport of nucleosides and therapeutic nucleoside analogs into the cells. Nucleosides 20-30 solute carrier family 28 member 3 Homo sapiens 46-51 20495821-7 2010 H(+)-coupled hCNT3 exhibited lower affinity for all natural nucleosides and different substrate selectivity compared to Na(+)-coupled hCNT3. Nucleosides 60-71 solute carrier family 28 member 3 Homo sapiens 13-18 20360301-6 2010 Recombinant hCNT3 mediated the transport of several known nucleoside substrates, and we identified 5-azacytidine as a new substrate for hCNT3. Nucleosides 58-68 solute carrier family 28 member 3 Homo sapiens 12-17 20172853-1 2010 Human concentrative nucleoside transporter-3 (hCNT3) is a sodium-coupled nucleoside transporter that exhibits high affinity and broad substrate selectivity, making it the most suitable candidate for mediating the uptake and cytotoxic action of most nucleoside-derived drugs. Nucleosides 20-30 solute carrier family 28 member 3 Homo sapiens 46-51 19494110-1 2009 Human concentrative nucleoside transporter, hCNT3, mediates Na+/nucleoside and H+/nucleoside co-transport. Nucleosides 20-30 solute carrier family 28 member 3 Homo sapiens 44-49 19494110-10 2009 In acid environments, including renal proximal tubule, H+/nucleoside co-transport may drive nucleoside accumulation by hCNT3. Nucleosides 58-68 solute carrier family 28 member 3 Homo sapiens 119-124 19494110-11 2009 Fusion of mNect to hCNT3 provided a simple, self-referencing, and effective way to monitor nucleoside transport, suggesting an approach that may have applications in assays of transport activity of other H(+)-coupled transport proteins. Nucleosides 91-101 solute carrier family 28 member 3 Homo sapiens 19-24 18500522-8 2009 Variations in hCNT3 abundance in renal proximal tubules, and hence nucleoside reabsorption, may explain interpatient variability in fludarabine"s pharmacokinetics and toxicities. Nucleosides 67-77 solute carrier family 28 member 3 Homo sapiens 14-19 19297449-7 2009 Collectively, this evidence suggested that apical hCNT3 and basolateral hENT2 are involved in proximal tubular reabsorption of adenosine and some nucleoside drugs and that apical hENT1 and basolateral hOATs are involved in proximal tubular secretion of 2"-deoxyadenosine. Nucleosides 146-156 solute carrier family 28 member 3 Homo sapiens 50-55 17993510-3 2008 The resulting hCNT3(C602R) protein has the same selectivity and affinity for natural nucleosides and nucleoside-derived drugs as hCNT3 but much lower concentrative capacity. Nucleosides 85-96 solute carrier family 28 member 3 Homo sapiens 14-19 18621735-1 2008 In humans, the SLC28 concentrative nucleoside transporter (CNT) protein family is represented by three Na+-coupled members; human CNT1 (hCNT1) and hCNT2 are pyrimidine and purine nucleoside-selective, respectively, whereas hCNT3 transports both purine and pyrimidine nucleosides and nucleoside drugs. Nucleosides 35-45 solute carrier family 28 member 3 Homo sapiens 223-228 18668436-2 2008 hCNT1 and hCNT2 translocate pyrimidine- and purine-nucleosides, respectively, by a sodium-dependent mechanism, whereas hCNT3 shows broad substrate selectivity and the unique ability of translocating nucleosides both in a sodium- and a proton-coupled manner. Nucleosides 199-210 solute carrier family 28 member 3 Homo sapiens 119-124 18827020-9 2009 We suggest that this novel hCNT3 isoform would be involved in the salvage of intracellular nucleosides from the lumen of the endoplasmic reticulum to the cytoplasm. Nucleosides 91-102 solute carrier family 28 member 3 Homo sapiens 27-32 17993510-7 2008 The sodium activation kinetic analysis of both transporters revealed a variation in the affinity for sodium and a shift in the Hill coefficient that could be consistent with a stoichiometry of 2:1 and 1:1 sodium/nucleoside, for hCNT3 and hCNT3(C602R), respectively. Nucleosides 212-222 solute carrier family 28 member 3 Homo sapiens 228-233 17993510-7 2008 The sodium activation kinetic analysis of both transporters revealed a variation in the affinity for sodium and a shift in the Hill coefficient that could be consistent with a stoichiometry of 2:1 and 1:1 sodium/nucleoside, for hCNT3 and hCNT3(C602R), respectively. Nucleosides 212-222 solute carrier family 28 member 3 Homo sapiens 238-243 17993510-9 2008 Individuals with the hCNT3(C602R) variant might show a lower nucleoside and nucleoside analog concentrative capacity, which could be clinically relevant. Nucleosides 61-71 solute carrier family 28 member 3 Homo sapiens 21-26 17993510-9 2008 Individuals with the hCNT3(C602R) variant might show a lower nucleoside and nucleoside analog concentrative capacity, which could be clinically relevant. Nucleosides 76-86 solute carrier family 28 member 3 Homo sapiens 21-26 17993510-3 2008 The resulting hCNT3(C602R) protein has the same selectivity and affinity for natural nucleosides and nucleoside-derived drugs as hCNT3 but much lower concentrative capacity. Nucleosides 85-95 solute carrier family 28 member 3 Homo sapiens 14-19 17409283-8 2007 The presence of 1) transcripts for hENT1/2 and hCNT1/2/3 and the hENT1 and hCNT3 proteins in human kidneys and 2) hENT1, hENT2, and hCNT3 activities in cultured proximal tubule cells suggest involvement of hENT1, hCNT3, and possibly also hENT2 in renal handling of nucleosides and nucleoside drugs. Nucleosides 265-276 solute carrier family 28 member 3 Homo sapiens 132-137 17412768-0 2007 Role of CNT3 in the transepithelial flux of nucleosides and nucleoside-derived drugs. Nucleosides 44-55 solute carrier family 28 member 3 Homo sapiens 8-12 17412768-0 2007 Role of CNT3 in the transepithelial flux of nucleosides and nucleoside-derived drugs. Nucleosides 44-54 solute carrier family 28 member 3 Homo sapiens 8-12 17412768-1 2007 We examined the role of the concentrative nucleoside transporter CNT3 in the establishment of a transepithelial flux of natural nucleosides and their pharmacologically active derivatives in renal epithelial cell lines. Nucleosides 128-139 solute carrier family 28 member 3 Homo sapiens 65-69 17412768-3 2007 hCNT3 was also identified in human kidney and its role in the transport of nucleosides was tested. Nucleosides 75-86 solute carrier family 28 member 3 Homo sapiens 0-5 17412768-5 2007 In these cells, hCNT3 was inserted into the apical membrane, thus generating, as for PCT cells, a transepithelial flux of both nucleosides and nucleoside-derived drugs. Nucleosides 127-138 solute carrier family 28 member 3 Homo sapiens 16-21 17412768-5 2007 In these cells, hCNT3 was inserted into the apical membrane, thus generating, as for PCT cells, a transepithelial flux of both nucleosides and nucleoside-derived drugs. Nucleosides 127-137 solute carrier family 28 member 3 Homo sapiens 16-21 17412768-8 2007 However release to the opposite compartment was CNT3 dependent, not only in terms of absolute flux (much higher when an apical CNT3 transporter was active) but also regarding metabolic transformations of the apically absorbed nucleosides. Nucleosides 226-237 solute carrier family 28 member 3 Homo sapiens 48-52 17412768-8 2007 However release to the opposite compartment was CNT3 dependent, not only in terms of absolute flux (much higher when an apical CNT3 transporter was active) but also regarding metabolic transformations of the apically absorbed nucleosides. Nucleosides 226-237 solute carrier family 28 member 3 Homo sapiens 127-131 17412768-9 2007 These results underline a critical role of CNT3 in the renal reabsorption of nucleosides and their derivatives as well as in their intracellular metabolism. Nucleosides 77-88 solute carrier family 28 member 3 Homo sapiens 43-47 17409283-8 2007 The presence of 1) transcripts for hENT1/2 and hCNT1/2/3 and the hENT1 and hCNT3 proteins in human kidneys and 2) hENT1, hENT2, and hCNT3 activities in cultured proximal tubule cells suggest involvement of hENT1, hCNT3, and possibly also hENT2 in renal handling of nucleosides and nucleoside drugs. Nucleosides 265-276 solute carrier family 28 member 3 Homo sapiens 132-137 17409283-8 2007 The presence of 1) transcripts for hENT1/2 and hCNT1/2/3 and the hENT1 and hCNT3 proteins in human kidneys and 2) hENT1, hENT2, and hCNT3 activities in cultured proximal tubule cells suggest involvement of hENT1, hCNT3, and possibly also hENT2 in renal handling of nucleosides and nucleoside drugs. Nucleosides 265-275 solute carrier family 28 member 3 Homo sapiens 132-137 17409283-8 2007 The presence of 1) transcripts for hENT1/2 and hCNT1/2/3 and the hENT1 and hCNT3 proteins in human kidneys and 2) hENT1, hENT2, and hCNT3 activities in cultured proximal tubule cells suggest involvement of hENT1, hCNT3, and possibly also hENT2 in renal handling of nucleosides and nucleoside drugs. Nucleosides 265-275 solute carrier family 28 member 3 Homo sapiens 132-137 17215872-7 2006 In renal epithelial cells, hCNT1, hCNT2, and hCNT3 at apical membranes, and hENT1 and hENT2 at basolateral membranes, apparently work in concert to mediate reabsorption of nucleosides from lumen to blood, driven by Na+ gradients. Nucleosides 172-183 solute carrier family 28 member 3 Homo sapiens 45-50 15738947-1 2005 The human concentrative nucleoside transporter, CNT3 (SLC28A3), plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs. Nucleosides 154-165 solute carrier family 28 member 3 Homo sapiens 48-52 15955867-1 2005 Human concentrative nucleoside transporters 1, 2, and 3 (hCNT1, hCNT2, and hCNT3) exhibit different functional characteristics, and a better understanding of their permeant selectivities is critical for development of nucleoside analog drugs with optimal pharmacokinetic properties. Nucleosides 20-30 solute carrier family 28 member 3 Homo sapiens 75-80 15870078-0 2005 The broadly selective human Na+/nucleoside cotransporter (hCNT3) exhibits novel cation-coupled nucleoside transport characteristics. Nucleosides 32-42 solute carrier family 28 member 3 Homo sapiens 58-63 15870078-6 2005 Na+ and H+ activated hCNT3 through mechanisms to increase nucleoside apparent binding affinity. Nucleosides 58-68 solute carrier family 28 member 3 Homo sapiens 21-26 15870078-7 2005 Direct and indirect methods demonstrated cation/nucleoside coupling stoichiometries of 2:1 in the presence of Na+ and both Na+ plus H+, but only 1:1 in the presence of H+ alone, suggesting that hCNT3 possesses two Na+-binding sites, only one of which is shared by H+. Nucleosides 48-58 solute carrier family 28 member 3 Homo sapiens 194-199 15861042-1 2005 INTRODUCTION: Human concentrative nucleoside transporter 3, hCNT3 (SLC28A3), which mediates transport of purine and pyrimidine nucleosides and a variety of antiviral and anticancer nucleoside drugs, was investigated to determine if there are single nucleotide polymorphisms in the coding regions of the hCNT3 gene. Nucleosides 34-44 solute carrier family 28 member 3 Homo sapiens 60-65 15861042-1 2005 INTRODUCTION: Human concentrative nucleoside transporter 3, hCNT3 (SLC28A3), which mediates transport of purine and pyrimidine nucleosides and a variety of antiviral and anticancer nucleoside drugs, was investigated to determine if there are single nucleotide polymorphisms in the coding regions of the hCNT3 gene. Nucleosides 34-44 solute carrier family 28 member 3 Homo sapiens 67-74 15861042-1 2005 INTRODUCTION: Human concentrative nucleoside transporter 3, hCNT3 (SLC28A3), which mediates transport of purine and pyrimidine nucleosides and a variety of antiviral and anticancer nucleoside drugs, was investigated to determine if there are single nucleotide polymorphisms in the coding regions of the hCNT3 gene. Nucleosides 34-44 solute carrier family 28 member 3 Homo sapiens 303-308 15738947-1 2005 The human concentrative nucleoside transporter, CNT3 (SLC28A3), plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs. Nucleosides 154-165 solute carrier family 28 member 3 Homo sapiens 54-61 15738947-1 2005 The human concentrative nucleoside transporter, CNT3 (SLC28A3), plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs. Nucleosides 24-34 solute carrier family 28 member 3 Homo sapiens 48-52 15738947-1 2005 The human concentrative nucleoside transporter, CNT3 (SLC28A3), plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs. Nucleosides 24-34 solute carrier family 28 member 3 Homo sapiens 54-61 34751688-8 2021 Our work provides a theoretical basis for the multistep elevator-like transportation mechanism of nucleosides, which helps to further understand the dynamic recognition between nucleoside substrates and hCNT3 as well as the design of nucleoside anticancer drugs. Nucleosides 98-109 solute carrier family 28 member 3 Homo sapiens 203-208 12856181-1 2004 The SLC28 family consists of three subtypes of sodium-dependent, concentrative nucleoside transporters, CNT1, CNT2, and CNT3 (SLC28A1, SLC28A2, and SLC28A3, respectively), that transport both naturally occurring nucleosides and synthetic nucleoside analogs used in the treatment of various diseases. Nucleosides 212-223 solute carrier family 28 member 3 Homo sapiens 120-124 14612157-2 2003 There are five known, functionally characterized nucleoside transporters with varying substrate specificities for nucleosides: concentrative nucleoside transporters (CNT1-CNT3; Solute Carrier (SLC) 28A1-28A3), which mediate the intracellular flux of nucleosides, and equilibrative nucleoside transporters (ENT1-ENT2; SLC29A1-SLC29A2), which mediate bi-directional facilitated diffusion of nucleosides. Nucleosides 114-125 solute carrier family 28 member 3 Homo sapiens 171-175 14612157-2 2003 There are five known, functionally characterized nucleoside transporters with varying substrate specificities for nucleosides: concentrative nucleoside transporters (CNT1-CNT3; Solute Carrier (SLC) 28A1-28A3), which mediate the intracellular flux of nucleosides, and equilibrative nucleoside transporters (ENT1-ENT2; SLC29A1-SLC29A2), which mediate bi-directional facilitated diffusion of nucleosides. Nucleosides 250-261 solute carrier family 28 member 3 Homo sapiens 171-175 14612157-2 2003 There are five known, functionally characterized nucleoside transporters with varying substrate specificities for nucleosides: concentrative nucleoside transporters (CNT1-CNT3; Solute Carrier (SLC) 28A1-28A3), which mediate the intracellular flux of nucleosides, and equilibrative nucleoside transporters (ENT1-ENT2; SLC29A1-SLC29A2), which mediate bi-directional facilitated diffusion of nucleosides. Nucleosides 250-261 solute carrier family 28 member 3 Homo sapiens 171-175 14645682-9 2003 The binding profiles identified in this study can be used to predict the potential transportability of nucleoside analogs, including anticancer or antiviral nucleoside drugs, by hCNT1 and hCNT3. Nucleosides 103-113 solute carrier family 28 member 3 Homo sapiens 188-193 14504928-2 2003 Previous expression studies in oocytes showed that the Km values for nucleosides of the cloned hCNT3 were 7- to 25-fold lower than the endogenous N3 transporter in HL60 cells. Nucleosides 69-80 solute carrier family 28 member 3 Homo sapiens 95-100 14504928-4 2003 We demonstrated that hCNT3 is a Na-dependent, broadly-selective nucleoside transporter with affinities (<11 microM) for nucleosides closely resembling the endogenous N3 transporter. Nucleosides 123-134 solute carrier family 28 member 3 Homo sapiens 21-26 12388627-9 2002 These data imply CNT3 may play a specialized role in nucleoside accumulation in milk and may identify an important role for PEPT2 and OATP-A transporters at the lactating mammary epithelium. Nucleosides 53-63 solute carrier family 28 member 3 Homo sapiens 17-21 12055084-1 2002 The human concentrative (Na+-linked) plasma membrane transport proteins hCNT1, hCNT2, and hCNT3 are pyrimidine nucleoside-selective (system cit), purine nucleoside-selective (system cif), or broadly selective for both pyrimidine and purine nucleosides (system cib), respectively. Nucleosides 240-251 solute carrier family 28 member 3 Homo sapiens 90-95 11396613-5 2001 hCNT3 and mCNT3 have primary structures that place them in a CNT subfamily separate from CNT1/2, transport a wide range of physiological pyrimidine and purine nucleosides and antineoplastic and antiviral nucleoside drugs (system cib), and exhibit a Na+:uridine coupling ratio of at least 2:1 (cf 1:1 for hCNT1/2). Nucleosides 159-170 solute carrier family 28 member 3 Homo sapiens 0-5 34751688-8 2021 Our work provides a theoretical basis for the multistep elevator-like transportation mechanism of nucleosides, which helps to further understand the dynamic recognition between nucleoside substrates and hCNT3 as well as the design of nucleoside anticancer drugs. Nucleosides 177-187 solute carrier family 28 member 3 Homo sapiens 203-208 34751688-8 2021 Our work provides a theoretical basis for the multistep elevator-like transportation mechanism of nucleosides, which helps to further understand the dynamic recognition between nucleoside substrates and hCNT3 as well as the design of nucleoside anticancer drugs. Nucleosides 234-244 solute carrier family 28 member 3 Homo sapiens 203-208 32875223-14 2020 The CNT3 protein, present in the microvilli of human vaginal epithelial cells, may have a role in redistributing nucleoside homologues delivered to the vaginal tract. Nucleosides 113-123 solute carrier family 28 member 3 Homo sapiens 4-8 32875223-15 2020 Transporter proteins such as CNT3 could shuttle nucleosides and their analogs through the vaginal epithelium to immune cells located in lower cell layers. Nucleosides 48-59 solute carrier family 28 member 3 Homo sapiens 29-33 32126230-1 2020 Humans possess three members of the cation-coupled concentrative nucleoside transporter CNT (SLC 28) family, hCNT1-3: hCNT1 is selective for pyrimidine nucleosides but also transports adenosine, hCNT2 transports purine nucleosides and uridine, and hCNT3 transports both pyrimidine and purine nucleosides. Nucleosides 152-163 solute carrier family 28 member 3 Homo sapiens 248-253 32126230-1 2020 Humans possess three members of the cation-coupled concentrative nucleoside transporter CNT (SLC 28) family, hCNT1-3: hCNT1 is selective for pyrimidine nucleosides but also transports adenosine, hCNT2 transports purine nucleosides and uridine, and hCNT3 transports both pyrimidine and purine nucleosides. Nucleosides 219-230 solute carrier family 28 member 3 Homo sapiens 248-253 32126230-1 2020 Humans possess three members of the cation-coupled concentrative nucleoside transporter CNT (SLC 28) family, hCNT1-3: hCNT1 is selective for pyrimidine nucleosides but also transports adenosine, hCNT2 transports purine nucleosides and uridine, and hCNT3 transports both pyrimidine and purine nucleosides. Nucleosides 219-230 solute carrier family 28 member 3 Homo sapiens 248-253 32126230-3 2020 By producing recombinant hCNT3 in Xenopus laevis oocytes, we have used radiochemical high performance liquid chromatography (HPLC) analysis to investigate the metabolic fate of transported [3H] or [14C] pyrimidine and purine nucleosides once inside cells. Nucleosides 225-236 solute carrier family 28 member 3 Homo sapiens 25-30