PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 28912101-2 2017 RasGRP1 possesses REM (Ras exchange), GEF (catalytic), EF-hand, C1, SuPT (suppressor of PT), and PT (plasma membrane-targeting) domains, among which the C1 domain drives membrane localization in response to diacylglycerol or phorbol ester and the PT domain recognizes phosphoinositides. Diglycerides 207-221 RAS guanyl releasing protein 1 Homo sapiens 0-7 23847619-2 2013 DAG triggers signaling by recruiting Ras guanyl-releasing protein 1, PKCtheta, and other effectors, whereas PA binds to effector molecules that include mechanistic target of rapamycin, Src homology region 2 domain-containing phosphatase 1, and Raf1. Diglycerides 0-3 RAS guanyl releasing protein 1 Homo sapiens 37-67 27891502-2 2016 Both DAG and PA are important second messengers cascading T cell receptor (TCR) signal by recruiting multiple effector molecules, such as RasGRP1, PKCtheta, and mTOR. Diglycerides 5-8 RAS guanyl releasing protein 1 Homo sapiens 138-145 27022025-1 2016 The C1 domain represents the recognition module for diacylglycerol and phorbol esters in protein kinase C, Ras guanine nucleotide releasing protein (RasGRP), and related proteins. Diglycerides 52-66 RAS guanyl releasing protein 1 Homo sapiens 107-147 27022025-1 2016 The C1 domain represents the recognition module for diacylglycerol and phorbol esters in protein kinase C, Ras guanine nucleotide releasing protein (RasGRP), and related proteins. Diglycerides 52-66 RAS guanyl releasing protein 1 Homo sapiens 149-155 24794745-0 2014 Synthesis, biological, and biophysical studies of DAG-indololactones designed as selective activators of RasGRP. Diglycerides 50-53 RAS guanyl releasing protein 1 Homo sapiens 105-111 28690878-2 2017 We hypothesized that the Treg-specific and activation-dependent reduced phosphorylation at Y523 allows binding of CalDAG GEFI to diacylglycerol, thereby impacting the formation of a Treg-specific immunological synapse. Diglycerides 129-143 RAS guanyl releasing protein 1 Homo sapiens 114-125 26549032-5 2016 In normal lymphocytes, RasGRP1 is recruited to the membrane by diacylglycerol (DAG) in a phospholipase C-gamma (PLCgamma)-dependent manner. Diglycerides 63-77 RAS guanyl releasing protein 1 Homo sapiens 23-30 26549032-5 2016 In normal lymphocytes, RasGRP1 is recruited to the membrane by diacylglycerol (DAG) in a phospholipase C-gamma (PLCgamma)-dependent manner. Diglycerides 79-82 RAS guanyl releasing protein 1 Homo sapiens 23-30 26549032-6 2016 Surprisingly, we find that leukemic RasGRP1-triggered Ras-Akt signals do not depend on acute activation of PLCgamma to generate DAG but rely on baseline DAG levels instead. Diglycerides 128-131 RAS guanyl releasing protein 1 Homo sapiens 36-43 26549032-6 2016 Surprisingly, we find that leukemic RasGRP1-triggered Ras-Akt signals do not depend on acute activation of PLCgamma to generate DAG but rely on baseline DAG levels instead. Diglycerides 153-156 RAS guanyl releasing protein 1 Homo sapiens 36-43 24280043-6 2013 RasGRP1, a key DAG-mediated activator of Ras signaling, associated to a greater extent with DGKzeta than with DGKalpha; however, in silico modeling of TCR-stimulated Ras activation suggested that a difference in RasGRP1 binding affinity was not sufficient to cause differences in the functions of each DGK isoform. Diglycerides 15-18 RAS guanyl releasing protein 1 Homo sapiens 0-7 19933860-2 2009 Previous studies reported that Ras guanyl nucleotide-releasing protein (RasGRP) 1, which is activated by diacylglycerol and Ca(2+), is crucial for TCR-mediated Ras-ERK activation. Diglycerides 105-119 RAS guanyl releasing protein 1 Homo sapiens 31-81 21775687-4 2011 Diacylglycerol (DAG) is an essential second messenger downstream of the TCR that activates the protein kinase C-IkappaB kinase (IKK)alpha/beta-NF-kappaB pathway, known to be crucial for iNKT development, as well as the RasGRP1-Ras-Erk1/2 pathway in T cells. Diglycerides 0-14 RAS guanyl releasing protein 1 Homo sapiens 219-226 21775687-4 2011 Diacylglycerol (DAG) is an essential second messenger downstream of the TCR that activates the protein kinase C-IkappaB kinase (IKK)alpha/beta-NF-kappaB pathway, known to be crucial for iNKT development, as well as the RasGRP1-Ras-Erk1/2 pathway in T cells. Diglycerides 16-19 RAS guanyl releasing protein 1 Homo sapiens 219-226 21285350-2 2011 The exchange factor RasGRP1 has a C1 domain that binds the lipid diacylglycerol and thus can potentially mediate membrane localization in response to receptors that are coupled to diacylglycerol-generating phospholipase Cs. Diglycerides 65-79 RAS guanyl releasing protein 1 Homo sapiens 20-27 21285350-2 2011 The exchange factor RasGRP1 has a C1 domain that binds the lipid diacylglycerol and thus can potentially mediate membrane localization in response to receptors that are coupled to diacylglycerol-generating phospholipase Cs. Diglycerides 180-194 RAS guanyl releasing protein 1 Homo sapiens 20-27 21285350-7 2011 Direct detection of phosphoinositides by the basic/hydrophobic cluster of RasGRP1 provides a novel mechanism for coupling and co-compartmentalizing phosphoinositide 3-kinase and Ras signaling and, in coordination with diacylglycerol detection by the C1 domain, gives RasGRP1 the potential to serve as an integrator of converging signals from the phosphoinositide 3-kinase and phospholipase C pathways. Diglycerides 218-232 RAS guanyl releasing protein 1 Homo sapiens 74-81 21138839-3 2011 One important for regulating activation levels of RasGRP is diacylglycerol (DAG), and its levels are influenced by diacylglycerol kinase-zeta (DGKzeta), which metabolizes DAG into phosphatidic acid, terminating DAG-mediated Ras signaling. Diglycerides 60-74 RAS guanyl releasing protein 1 Homo sapiens 50-56 21138839-3 2011 One important for regulating activation levels of RasGRP is diacylglycerol (DAG), and its levels are influenced by diacylglycerol kinase-zeta (DGKzeta), which metabolizes DAG into phosphatidic acid, terminating DAG-mediated Ras signaling. Diglycerides 76-79 RAS guanyl releasing protein 1 Homo sapiens 50-56 21138839-3 2011 One important for regulating activation levels of RasGRP is diacylglycerol (DAG), and its levels are influenced by diacylglycerol kinase-zeta (DGKzeta), which metabolizes DAG into phosphatidic acid, terminating DAG-mediated Ras signaling. Diglycerides 171-174 RAS guanyl releasing protein 1 Homo sapiens 50-56 21138839-3 2011 One important for regulating activation levels of RasGRP is diacylglycerol (DAG), and its levels are influenced by diacylglycerol kinase-zeta (DGKzeta), which metabolizes DAG into phosphatidic acid, terminating DAG-mediated Ras signaling. Diglycerides 171-174 RAS guanyl releasing protein 1 Homo sapiens 50-56 21966541-0 2011 Basal LAT-diacylglycerol-RasGRP1 signals in T cells maintain TCRalpha gene expression. Diglycerides 10-24 RAS guanyl releasing protein 1 Homo sapiens 25-32 21966541-5 2011 Independent approaches of reducing basal activity through the LAT-diacylglycerol-RasGRP pathway led to reduced constitutive Ras-MEK-ERK signals and decreased TCRalpha mRNA and surface TCR expression in Jurkat cells. Diglycerides 66-80 RAS guanyl releasing protein 1 Homo sapiens 81-87 21966541-9 2011 We postulate that basal LAT-diacylglycerol-RasGRP signals fulfill a regulatory function in peripheral T lymphocytes by maintaining proper gene expression programs. Diglycerides 28-42 RAS guanyl releasing protein 1 Homo sapiens 43-49 19896467-10 2009 Basal phospho-Akt was also decreased by treatment with Go6976, an inhibitor of conventional protein kinase C and protein kinase D. While the proposed RasGRP-Ras-PI3K-Akt signaling axis may operate in some situations, our results indicate that alternative links between DAG targets such as protein kinases and the PI3K signaling system are more prominent. Diglycerides 269-272 RAS guanyl releasing protein 1 Homo sapiens 150-156 23589333-6 2013 In agreement with RasGRP allosterically priming SOS, exponential ERK activation is severely decreased by pharmacological or genetic perturbation of the phospholipase Cgamma (PLCgamma)-diacylglycerol-RasGRP1 pathway. Diglycerides 184-198 RAS guanyl releasing protein 1 Homo sapiens 18-24 18077438-6 2007 Here we describe how one of those alternate targets of DAG/PE effects, Ras guanyl-releasing protein 1 (RasGRP1), mediates the PE-induced suppression of function and the surface expression of NCC. Diglycerides 55-58 RAS guanyl releasing protein 1 Homo sapiens 71-101 18263588-1 2008 C1 domains mediate the recognition and subsequent signaling response to diacylglycerol and phorbol esters by protein kinase C (PKC) and by several other families of signal-transducing proteins such as the chimerins or RasGRP. Diglycerides 72-86 RAS guanyl releasing protein 1 Homo sapiens 218-224 19628710-3 2009 We here identify Ca(2+) and DAG-regulated guanine nucleotide exchange factor I (CalDAG-GEFI) as a critical molecule in Ca(2+)-dependent platelet activation. Diglycerides 28-31 RAS guanyl releasing protein 1 Homo sapiens 80-91 19100522-6 2009 RESULTS: Stimulation of B cells with DAG analogues results in activation of protein kinase C/RasGRP-Ras-Raf-Mek-Erk signaling and phosphorylation of the proapoptotic BH3-only protein Bim. Diglycerides 37-40 RAS guanyl releasing protein 1 Homo sapiens 93-99 18174897-5 2008 Diminished Ras activation can, in part, be explained by the upregulated expression of diacylglycerol kinases (DGKs), which phosphorylate diacylglycerol and restrict Ras guanyl releasing protein 1 (RasGRP1)-dependent activation of Ras. Diglycerides 86-100 RAS guanyl releasing protein 1 Homo sapiens 165-195 18174897-5 2008 Diminished Ras activation can, in part, be explained by the upregulated expression of diacylglycerol kinases (DGKs), which phosphorylate diacylglycerol and restrict Ras guanyl releasing protein 1 (RasGRP1)-dependent activation of Ras. Diglycerides 86-100 RAS guanyl releasing protein 1 Homo sapiens 197-204 18077438-6 2007 Here we describe how one of those alternate targets of DAG/PE effects, Ras guanyl-releasing protein 1 (RasGRP1), mediates the PE-induced suppression of function and the surface expression of NCC. Diglycerides 55-58 RAS guanyl releasing protein 1 Homo sapiens 103-110 17567957-2 2007 RasGRP1 contains a diacylglycerol-binding C1 domain, and it has been assumed that this domain is entirely responsible for RasGRP1 translocation. Diglycerides 19-33 RAS guanyl releasing protein 1 Homo sapiens 0-7 17523924-2 2007 All RasGRP family members contain C1 domains which, in other proteins, bind DAG (diacylglycerol) and thus mediate the proximal signal-transduction events induced by this lipid second messenger. Diglycerides 76-79 RAS guanyl releasing protein 1 Homo sapiens 4-10 17523924-2 2007 All RasGRP family members contain C1 domains which, in other proteins, bind DAG (diacylglycerol) and thus mediate the proximal signal-transduction events induced by this lipid second messenger. Diglycerides 81-95 RAS guanyl releasing protein 1 Homo sapiens 4-10 17523924-6 2007 In cells, the isolated C1 domains of RasGRP1, RasGRP3 and RasGRP4alpha co-localize with membranes and relocalize in response to DAG, whereas the C1 domains of RasGRP2 and RasGRP4beta do not. Diglycerides 128-131 RAS guanyl releasing protein 1 Homo sapiens 37-44 17523924-7 2007 Only the C1 domains of RasGRP1, RasGRP3 and RasGRP4alpha recognize DAG as a ligand within phospholipid vesicles and do so with differential affinities. Diglycerides 67-70 RAS guanyl releasing protein 1 Homo sapiens 23-30 17567957-2 2007 RasGRP1 contains a diacylglycerol-binding C1 domain, and it has been assumed that this domain is entirely responsible for RasGRP1 translocation. Diglycerides 19-33 RAS guanyl releasing protein 1 Homo sapiens 122-129 17567957-6 2007 By binding to diacylglycerol generated by BCR-coupled phospholipase Cgamma2, the C1 domain counteracts the SuPT domain and enables efficient RasGRP1 translocation to the plasma membrane. Diglycerides 14-28 RAS guanyl releasing protein 1 Homo sapiens 141-148 15923197-1 2005 Although multiple natural products are potent ligands for the diacylglycerol binding C1 domain of protein kinase C (PKC), RasGRP, and related targets, the high conservation of C1 domains has impeded the development of selective ligands. Diglycerides 62-76 RAS guanyl releasing protein 1 Homo sapiens 122-128 17486117-4 2007 Ras activation on both compartments required RasGRP1, an exchange factor regulated by calcium and diacylglycerol (DAG), but phospholipase C (PLC) activity was required only for activation on the Golgi. Diglycerides 98-112 RAS guanyl releasing protein 1 Homo sapiens 45-52 17486117-4 2007 Ras activation on both compartments required RasGRP1, an exchange factor regulated by calcium and diacylglycerol (DAG), but phospholipase C (PLC) activity was required only for activation on the Golgi. Diglycerides 114-117 RAS guanyl releasing protein 1 Homo sapiens 45-52 17052215-3 2006 RasGRP1 also possesses a DAG (diacylglycerol)-binding C1 domain and a pair of EF hands that bind calcium. Diglycerides 25-28 RAS guanyl releasing protein 1 Homo sapiens 0-7 17052215-3 2006 RasGRP1 also possesses a DAG (diacylglycerol)-binding C1 domain and a pair of EF hands that bind calcium. Diglycerides 30-44 RAS guanyl releasing protein 1 Homo sapiens 0-7 16042567-5 2005 We mapped one pathway responsible for this activity as involving PLCgamma (phospholipase Cgamma)/DAG (diacylglycerol)+Ca2+/RasGRP1. Diglycerides 102-116 RAS guanyl releasing protein 1 Homo sapiens 123-130 15657177-8 2005 After stimulation of T-cell receptor (TCR) or DAG analog treatment of T cells, PKC-catalyzed phosphorylation of RasGRP1 occurs on the homologous residue, Thr184. Diglycerides 46-49 RAS guanyl releasing protein 1 Homo sapiens 112-119 15657177-2 2005 DAG recruits both protein kinase C (PKC) and RasGRP family members to the membrane and contributes to their activation. Diglycerides 0-3 RAS guanyl releasing protein 1 Homo sapiens 45-51 15899849-2 2005 In T lymphocytes, Ras can be activated by two Ras exchange factors, SOS and RasGRP1, which are recruited through the adapters Grb2 and LAT and via the second-messenger diacylglycerol (DAG), respectively. Diglycerides 168-182 RAS guanyl releasing protein 1 Homo sapiens 76-83 15899849-2 2005 In T lymphocytes, Ras can be activated by two Ras exchange factors, SOS and RasGRP1, which are recruited through the adapters Grb2 and LAT and via the second-messenger diacylglycerol (DAG), respectively. Diglycerides 184-187 RAS guanyl releasing protein 1 Homo sapiens 76-83 15899849-8 2005 RasGRP1 relies on its DAG-binding domain to selectively activate Erk kinases. Diglycerides 22-25 RAS guanyl releasing protein 1 Homo sapiens 0-7 15899849-12 2005 Last, DAG-PKC-RasGRP1-driven Ras-Erk activation in T cells is a unique signaling event, not simply compensated for by SOS activity. Diglycerides 6-9 RAS guanyl releasing protein 1 Homo sapiens 14-21 15657177-9 2005 These studies shed light on the proposed "PKC-Ras pathway" and support the hypothesis that RasGRP phosphorylation by PKC is a mechanism that integrates DAG signaling systems in T and B cells. Diglycerides 152-155 RAS guanyl releasing protein 1 Homo sapiens 91-97 15064353-0 2004 Diacylglycerol-dependent binding recruits PKCtheta and RasGRP1 C1 domains to specific subcellular localizations in living T lymphocytes. Diglycerides 0-14 RAS guanyl releasing protein 1 Homo sapiens 55-62 14583629-0 2004 Diacylglycerols containing Omega 3 and Omega 6 fatty acids bind to RasGRP and modulate MAP kinase activation. Diglycerides 0-15 RAS guanyl releasing protein 1 Homo sapiens 67-73 14702343-9 2004 Furthermore, when expressed in Jurkat cells, CalDAG-GEFI, a calcium and diacylglycerol-responsive Rap1 exchange factor, associated with Rap1, and resulted in enhanced Rap1 activation and adhesion triggered by the TCR. Diglycerides 72-86 RAS guanyl releasing protein 1 Homo sapiens 45-56 14583629-7 2004 The inhibition of production of DAG-AA and DAG-DHA significantly inhibited MAP kinase activation in RasGRP overexpressing, but not in control, cells. Diglycerides 32-35 RAS guanyl releasing protein 1 Homo sapiens 100-106 14583629-7 2004 The inhibition of production of DAG-AA and DAG-DHA significantly inhibited MAP kinase activation in RasGRP overexpressing, but not in control, cells. Diglycerides 43-46 RAS guanyl releasing protein 1 Homo sapiens 100-106 14583629-8 2004 Our study demonstrates that three DAG molecular species bind to RasGRP, but only DAG-AA and DAG-DHA participate in the modulation of RasGRP-mediated activation of MAP kinases in Jurkat T cells. Diglycerides 34-37 RAS guanyl releasing protein 1 Homo sapiens 64-70 14583629-8 2004 Our study demonstrates that three DAG molecular species bind to RasGRP, but only DAG-AA and DAG-DHA participate in the modulation of RasGRP-mediated activation of MAP kinases in Jurkat T cells. Diglycerides 81-84 RAS guanyl releasing protein 1 Homo sapiens 133-139 14583629-8 2004 Our study demonstrates that three DAG molecular species bind to RasGRP, but only DAG-AA and DAG-DHA participate in the modulation of RasGRP-mediated activation of MAP kinases in Jurkat T cells. Diglycerides 81-84 RAS guanyl releasing protein 1 Homo sapiens 133-139 14583629-2 2004 The competition studies with these DAGs on [3H]PDBu binding to RasGRP revealed different Ki values for these DAG molecular species. Diglycerides 35-39 RAS guanyl releasing protein 1 Homo sapiens 63-69 14583629-2 2004 The competition studies with these DAGs on [3H]PDBu binding to RasGRP revealed different Ki values for these DAG molecular species. Diglycerides 35-38 RAS guanyl releasing protein 1 Homo sapiens 63-69 11919165-6 2002 These results suggest that, in T cells, agonist-stimulated DAG generation is the key factor controlling activation of the Ras/MAPK signaling pathway through membrane translocation of RasGRP. Diglycerides 59-62 RAS guanyl releasing protein 1 Homo sapiens 183-189 12070163-4 2002 DAG activates RasGRP and protein kinase C theta. Diglycerides 0-3 RAS guanyl releasing protein 1 Homo sapiens 14-20 12070163-5 2002 Because both RasGRP and protein kinase C theta are essential for thymocyte and T cell function, it is critical to understand how DAG is regulated. Diglycerides 129-132 RAS guanyl releasing protein 1 Homo sapiens 13-19 12091553-8 2002 This low-molecular-weight G protein is activated following DAG binding to Ras-GRP, a neuronal Ras-GTP exchange factor. Diglycerides 59-62 RAS guanyl releasing protein 1 Homo sapiens 74-81 12730099-1 2003 Members of the RasGRP family of Ras activators have C1 domains that bind diacylglycerol (DAG) and DAG analogs such as the tumor-promoting phorbol esters. Diglycerides 73-87 RAS guanyl releasing protein 1 Homo sapiens 15-21 12730099-1 2003 Members of the RasGRP family of Ras activators have C1 domains that bind diacylglycerol (DAG) and DAG analogs such as the tumor-promoting phorbol esters. Diglycerides 89-92 RAS guanyl releasing protein 1 Homo sapiens 15-21 12730099-1 2003 Members of the RasGRP family of Ras activators have C1 domains that bind diacylglycerol (DAG) and DAG analogs such as the tumor-promoting phorbol esters. Diglycerides 98-101 RAS guanyl releasing protein 1 Homo sapiens 15-21 12730099-2 2003 RasGRP members could be responsible for some of the DAG signaling processes that have previously been attributed to protein kinase C (PKC). Diglycerides 52-55 RAS guanyl releasing protein 1 Homo sapiens 0-6 12730099-9 2003 These results provide the first indication for a functional interaction between a RasGRP family member and a dissimilar DAG binding protein. Diglycerides 120-123 RAS guanyl releasing protein 1 Homo sapiens 82-88 12414987-6 2002 RasGRP1 (in Ras/Raf/MEK/ERK signalling) and Munc13-1 (in neurotransmitter secretion) are examples of non-PKC diacylglycerol/phorbol-ester receptors that mediate diacylglycerol and phorbol-ester effects originally thought to be caused by PKC isozymes. Diglycerides 109-123 RAS guanyl releasing protein 1 Homo sapiens 0-7 12414987-6 2002 RasGRP1 (in Ras/Raf/MEK/ERK signalling) and Munc13-1 (in neurotransmitter secretion) are examples of non-PKC diacylglycerol/phorbol-ester receptors that mediate diacylglycerol and phorbol-ester effects originally thought to be caused by PKC isozymes. Diglycerides 161-175 RAS guanyl releasing protein 1 Homo sapiens 0-7 12414987-8 2002 The examples of RasGRP1 and Munc13-1 show that detailed genetic analyses of C(1)-domain-containing non-PKC diacylglycerol/phorbol-ester receptors in mammals are ideally suited to achieve this goal. Diglycerides 107-121 RAS guanyl releasing protein 1 Homo sapiens 16-23 11503142-1 2001 CalDAG-GEFI and CalDAG-GEFII (identical to RasGRP) are novel, brain-enriched guanine nucleotide exchange factors (GEFs) that can be stimulated by calcium and diacylglycerol and that can activate small GTPases, including Ras and Rap1, molecules increasingly recognized as having signaling functions in neurons. Diglycerides 158-172 RAS guanyl releasing protein 1 Homo sapiens 0-11 11831896-3 2002 One interesting feature of RasGRP is the presence of a C-terminal C1 domain, which has high homology to the PKC C1 domain and binds to diacylglycerol (DAG) and phorbol esters. Diglycerides 135-149 RAS guanyl releasing protein 1 Homo sapiens 27-33 11831896-3 2002 One interesting feature of RasGRP is the presence of a C-terminal C1 domain, which has high homology to the PKC C1 domain and binds to diacylglycerol (DAG) and phorbol esters. Diglycerides 151-154 RAS guanyl releasing protein 1 Homo sapiens 27-33 11503142-1 2001 CalDAG-GEFI and CalDAG-GEFII (identical to RasGRP) are novel, brain-enriched guanine nucleotide exchange factors (GEFs) that can be stimulated by calcium and diacylglycerol and that can activate small GTPases, including Ras and Rap1, molecules increasingly recognized as having signaling functions in neurons. Diglycerides 158-172 RAS guanyl releasing protein 1 Homo sapiens 16-28 11503142-1 2001 CalDAG-GEFI and CalDAG-GEFII (identical to RasGRP) are novel, brain-enriched guanine nucleotide exchange factors (GEFs) that can be stimulated by calcium and diacylglycerol and that can activate small GTPases, including Ras and Rap1, molecules increasingly recognized as having signaling functions in neurons. Diglycerides 158-172 RAS guanyl releasing protein 1 Homo sapiens 43-49 11257115-8 2001 Coimmunoprecipitation of DGK zeta and RasGRP was enhanced in the presence of phorbol esters, which are DAG analogues that cannot be metabolized by DGKs, suggesting that DAG signaling can induce their interaction. Diglycerides 103-106 RAS guanyl releasing protein 1 Homo sapiens 38-44 11257115-8 2001 Coimmunoprecipitation of DGK zeta and RasGRP was enhanced in the presence of phorbol esters, which are DAG analogues that cannot be metabolized by DGKs, suggesting that DAG signaling can induce their interaction. Diglycerides 169-172 RAS guanyl releasing protein 1 Homo sapiens 38-44 11257115-10 2001 Thus, we have identified a novel way to regulate Ras activation: through DGK zeta, which controls local accumulation of DAG that would otherwise activate RasGRP. Diglycerides 120-123 RAS guanyl releasing protein 1 Homo sapiens 154-160 9789079-5 1998 We now report the cloning of a brain-enriched gene, CalDAG-GEFI, which has substrate specificity for Rap1A, dual binding domains for calcium (Ca2+) and diacylglycerol (DAG), and enriched expression in brain basal ganglia pathways and their axon-terminal regions. Diglycerides 152-166 RAS guanyl releasing protein 1 Homo sapiens 52-63 10807788-3 2000 Here we show that RasGRP, a Ras activator that contains calcium-binding EF hands and a DAG-binding domain, is expressed in T cells. Diglycerides 87-90 RAS guanyl releasing protein 1 Homo sapiens 18-24 10807788-6 2000 Overexpression of RasGRP in T cells enhanced TCR-Ras-Erk signaling and augmented interleukin-2 secretion in response to calcium ionophore plus DAG analogues phorbol ester myristate or bryostatin-1. Diglycerides 143-146 RAS guanyl releasing protein 1 Homo sapiens 18-24 10779365-0 2000 The guanine nucleotide exchange factor RasGRP is a high -affinity target for diacylglycerol and phorbol esters. Diglycerides 77-91 RAS guanyl releasing protein 1 Homo sapiens 39-45 10779365-10 2000 We conclude that RasGRP is a high affinity receptor for phorbol esters and diacylglycerol. Diglycerides 75-89 RAS guanyl releasing protein 1 Homo sapiens 17-23 10779365-11 2000 RasGRP thus provides a direct link between diacylglycerol generation or phorbol ester/bryostatin treatment and Ras activation. Diglycerides 43-57 RAS guanyl releasing protein 1 Homo sapiens 0-6 9582122-0 1998 RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs. Diglycerides 69-83 RAS guanyl releasing protein 1 Homo sapiens 0-6 9582122-2 1998 Besides the catalytic domain, RasGRP has an atypical pair of "EF hands" that bind calcium and a diacylglycerol (DAG)-binding domain. Diglycerides 96-110 RAS guanyl releasing protein 1 Homo sapiens 30-36 9582122-2 1998 Besides the catalytic domain, RasGRP has an atypical pair of "EF hands" that bind calcium and a diacylglycerol (DAG)-binding domain. Diglycerides 112-115 RAS guanyl releasing protein 1 Homo sapiens 30-36 9582122-7 1998 RasGRP is expressed in the nervous system, where it may couple changes in DAG and possibly calcium concentrations to Ras activation. Diglycerides 74-77 RAS guanyl releasing protein 1 Homo sapiens 0-6 34529720-6 2021 Single-cell and bulk RNA-seq analyses revealed that of the four known isoforms of the Ras guanine nucleotide exchange factor RasGRP, RasGRP1 is by far the predominantly expressed diacylglycerol-dependent isoform in CD4+ T cells. Diglycerides 179-193 RAS guanyl releasing protein 1 Homo sapiens 125-131 34529720-6 2021 Single-cell and bulk RNA-seq analyses revealed that of the four known isoforms of the Ras guanine nucleotide exchange factor RasGRP, RasGRP1 is by far the predominantly expressed diacylglycerol-dependent isoform in CD4+ T cells. Diglycerides 179-193 RAS guanyl releasing protein 1 Homo sapiens 133-140