PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 7690078-4 1993 Cell-associated IL-1 activity was measured after fixation by paraformaldehyde (PFA). paraform 61-77 interleukin 1 complex Mus musculus 16-20 14688369-7 2004 D10 cell proliferation assay revealed MA-IL-1 bioactivity of paraformaldehyde-fixed synoviocytes and the further induction of endogenous mouse MA-IL-1 via autocrine mechanisms. paraform 61-77 interleukin 1 complex Mus musculus 41-45 7690078-4 1993 Cell-associated IL-1 activity was measured after fixation by paraformaldehyde (PFA). paraform 79-82 interleukin 1 complex Mus musculus 16-20 7690078-6 1993 On the other hand, cell-associated IL-1 was detected in AM fixed by PFA on days 1, 3, 6 and 9 after administration. paraform 68-71 interleukin 1 complex Mus musculus 35-39 2839894-2 1988 The data reported here show that although the levels of secreted IL-1 were equally high after in vitro stimulation with an optimal dose of LPS or silica, there were two clear differences: (i) the levels of membrane-associated IL-1 (as detected by the comitogenic effect of paraformaldehyde (PFA)-fixed cells or purified membrane fragments on murine thymocytes) were ca. paraform 273-289 interleukin 1 complex Mus musculus 226-230 2801312-2 1989 Membrane IL-1 activity was measured on paraformaldehyde fixed peritoneal macrophages using EL4 6.1 lymphocyte cell line. paraform 39-55 interleukin 1 complex Mus musculus 9-13 1739981-11 1992 The inability to detect IL-1 by the thymocyte assay is due to the presence of a chondrocyte inhibitor of IL-1 that can be demonstrated in cell sonicates, supernatants, and on paraformaldehyde-fixed chondrocytes. paraform 175-191 interleukin 1 complex Mus musculus 105-109 3263922-0 1988 Studies on the release of cell-associated interleukin 1 by paraformaldehyde-treated murine macrophages. paraform 59-75 interleukin 1 complex Mus musculus 42-55 3263922-1 1988 Experiments are presented demonstrating the release of interleukin 1 (IL1) by lipopolysaccharide-stimulated murine macrophages (M phi) after treatment of the cells with paraformaldehyde (PFA). paraform 169-185 interleukin 1 complex Mus musculus 55-68 3263922-1 1988 Experiments are presented demonstrating the release of interleukin 1 (IL1) by lipopolysaccharide-stimulated murine macrophages (M phi) after treatment of the cells with paraformaldehyde (PFA). paraform 169-185 interleukin 1 complex Mus musculus 70-73 3263922-1 1988 Experiments are presented demonstrating the release of interleukin 1 (IL1) by lipopolysaccharide-stimulated murine macrophages (M phi) after treatment of the cells with paraformaldehyde (PFA). paraform 187-190 interleukin 1 complex Mus musculus 55-68 3263922-1 1988 Experiments are presented demonstrating the release of interleukin 1 (IL1) by lipopolysaccharide-stimulated murine macrophages (M phi) after treatment of the cells with paraformaldehyde (PFA). paraform 187-190 interleukin 1 complex Mus musculus 70-73 3263922-5 1988 Addition of leupeptin to PFA-treated M phi also reduced the amount of released IL1 activity, suggesting that proteolytic modification of the IL1 alpha precursor is involved in the generation of biological active IL1 alpha. paraform 25-28 interleukin 1 complex Mus musculus 79-82 2839894-2 1988 The data reported here show that although the levels of secreted IL-1 were equally high after in vitro stimulation with an optimal dose of LPS or silica, there were two clear differences: (i) the levels of membrane-associated IL-1 (as detected by the comitogenic effect of paraformaldehyde (PFA)-fixed cells or purified membrane fragments on murine thymocytes) were ca. paraform 291-294 interleukin 1 complex Mus musculus 226-230 3495584-1 1987 IL 1 activity, as assayed by the proliferation of responsive mouse thymocytes and a human astrocytoma cell line, was detected on the membrane of 1% paraformaldehyde-fixed activated human monocytes. paraform 148-164 interleukin 1 complex Mus musculus 0-4 2946770-8 1986 In the second, T cells stimulated by paraformaldehyde-fixed macrophages in the presence of concanavalin A or antigen secreted a soluble factor that induced macrophage mIL 1 expression. paraform 37-53 interleukin 1 complex Mus musculus 167-172 3112227-3 1987 The findings that i) PFA-fixed Mo did not produce or release IL 1, ii) the accessory function of PFA-fixed Mo could be inhibited with pretreatment with anti-IL 1 antibody, iii) PFA-fixed Mo had a comitogenic effect in the murine thymocyte assay, and iv) there was a temporal difference between the capacities to function in the comitogenic assay and to produce soluble IL 1, suggest that human Mo can express membrane-associated IL 1 and that it is functionally relevant. paraform 97-100 interleukin 1 complex Mus musculus 157-161 3112227-3 1987 The findings that i) PFA-fixed Mo did not produce or release IL 1, ii) the accessory function of PFA-fixed Mo could be inhibited with pretreatment with anti-IL 1 antibody, iii) PFA-fixed Mo had a comitogenic effect in the murine thymocyte assay, and iv) there was a temporal difference between the capacities to function in the comitogenic assay and to produce soluble IL 1, suggest that human Mo can express membrane-associated IL 1 and that it is functionally relevant. paraform 97-100 interleukin 1 complex Mus musculus 157-161 3112227-3 1987 The findings that i) PFA-fixed Mo did not produce or release IL 1, ii) the accessory function of PFA-fixed Mo could be inhibited with pretreatment with anti-IL 1 antibody, iii) PFA-fixed Mo had a comitogenic effect in the murine thymocyte assay, and iv) there was a temporal difference between the capacities to function in the comitogenic assay and to produce soluble IL 1, suggest that human Mo can express membrane-associated IL 1 and that it is functionally relevant. paraform 97-100 interleukin 1 complex Mus musculus 157-161 3112227-3 1987 The findings that i) PFA-fixed Mo did not produce or release IL 1, ii) the accessory function of PFA-fixed Mo could be inhibited with pretreatment with anti-IL 1 antibody, iii) PFA-fixed Mo had a comitogenic effect in the murine thymocyte assay, and iv) there was a temporal difference between the capacities to function in the comitogenic assay and to produce soluble IL 1, suggest that human Mo can express membrane-associated IL 1 and that it is functionally relevant. paraform 97-100 interleukin 1 complex Mus musculus 157-161 2936823-10 1986 The finding that IL 1 enhanced T cell responses to PFA-treated or UV-irradiated TD-DC in the absence and in the presence of excess IL 2 indicates that loss of stimulatory activity of TD-DC may be due in part to loss or inactivation of IL 1. paraform 51-54 interleukin 1 complex Mus musculus 17-21