PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24867629-4 2014 By replacing Val248 and Thr251 with cysteines to create a disulfide bridge, the recombinant lipases reE-PcLipV248C-T251C (expressed in E. coli) and reP-PcLipV248C-T251C (expressed in P. pastoris) were obtained. Disulfides 58-67 replication protein Escherichia coli 148-151