PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10464282-2 1999 Comparison of protein C activation in the presence and absence of TM identified 11 residues mediating the thrombin-TM interaction (Lys(21), Gln(24), Arg(62), Lys(65), His(66), Arg(68), Thr(69), Tyr(71), Arg(73), Lys(77), Lys(106)). Histidine 167-170 coagulation factor II, thrombin Homo sapiens 106-114 10373475-2 1999 Thrombin exosite interactions with human factor V and its activation products were quantitatively characterized in equilibrium binding studies based on fluorescence changes of thrombin covalently labeled with 2-anilinonaphthalene-6-sulfonic acid (ANS) linked to the catalytic site histidine residue by Nalpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CH2Cl ([ANS]FPR-thrombin). Histidine 281-290 coagulation factor II, thrombin Homo sapiens 0-8 8805707-6 1996 The structure reveals a fold that has not been reported for any other serine protease, and an active site consisting of a novel catalytic triad in which the third member is a histidine instead of an aspartic acid, or possibly a catalytic tetrad consisting of a serine, two histidines and an aspartic acid. Histidine 175-184 coagulation factor II, thrombin Homo sapiens 70-85 9575335-4 1998 Some of them use a catalytic Cys/His/Glu triad similar to serine protease, whereas the aminoterminal cysteine of the others is believed to play the same function. Histidine 33-36 coagulation factor II, thrombin Homo sapiens 58-73 8805707-6 1996 The structure reveals a fold that has not been reported for any other serine protease, and an active site consisting of a novel catalytic triad in which the third member is a histidine instead of an aspartic acid, or possibly a catalytic tetrad consisting of a serine, two histidines and an aspartic acid. Histidine 273-283 coagulation factor II, thrombin Homo sapiens 70-85 8360880-4 1993 Ser-205, along with His-43 and Asp-99 make up the catalytic triad within the active site of thrombin. Histidine 20-23 coagulation factor II, thrombin Homo sapiens 92-100 8664285-8 1996 In ligand blot analysis, thrombin blotted the His-1-Glu-282 fragment, but not His-1-Leu-275. Histidine 46-49 coagulation factor II, thrombin Homo sapiens 25-33 8745399-5 1996 Although PPACK forms covalent bonds to both serine and the histidine of the catalytic triad of thrombin, neither BMS-186282 nor BMS-189090 bind covalently and only BMS-186282 forms a hydrogen bond to the serine of the catalytic triad. Histidine 59-68 coagulation factor II, thrombin Homo sapiens 95-103 8749307-0 1995 Identification of the reactive histidine of cucumisin, a plant serine protease: modification with peptidyl chloromethyl ketone derivative of peptide substrate. Histidine 31-40 coagulation factor II, thrombin Homo sapiens 63-78 8557169-3 1995 The proprotein convertases are all dependent on calcium for activity and all possess highly conserved subtilisin-like domains with the characteristic catalytic triad of this serine protease (ordered Asp, His, and Ser along the polypeptide chain). Histidine 204-207 coagulation factor II, thrombin Homo sapiens 174-189 8664285-16 1996 These findings indicate that the sulfated tyrosine/anionic GP Ibalpha residues Tyr-276-Glu-282 are important for the binding of thrombin and botrocetin-dependent binding of thrombin and the botrocetin-dependent binding of vWF, but that vWF also interacts with residues within His-1-Leu-275. Histidine 276-279 coagulation factor II, thrombin Homo sapiens 128-136 8664285-16 1996 These findings indicate that the sulfated tyrosine/anionic GP Ibalpha residues Tyr-276-Glu-282 are important for the binding of thrombin and botrocetin-dependent binding of thrombin and the botrocetin-dependent binding of vWF, but that vWF also interacts with residues within His-1-Leu-275. Histidine 276-279 coagulation factor II, thrombin Homo sapiens 173-181 7543025-4 1995 We now establish that phosphorylation following platelet activation with thrombin or collagen generates phosphohistidine at histidines on the cytoplasmic tail of P-selectin. Histidine 124-134 coagulation factor II, thrombin Homo sapiens 73-81 8054455-7 1994 This mutation results in the amino acid substitution Arg-->His at position 16 of the A alpha chain, the site of thrombin cleavage. Histidine 62-65 coagulation factor II, thrombin Homo sapiens 115-123 3886645-0 1985 Modification of histidines in human prothrombin. Histidine 16-26 coagulation factor II, thrombin Homo sapiens 36-47 8470887-5 1993 In contrast, binding of thrombin to p-CBA agarose was eliminated by modification of the active site histidine using either H-D-phenylalanyl-L-prolyl-L-arginine chloromethylketone or dansyl-L-glutamyl-glycyl-L-arginine chloromethylketone but not with tosyl-L-lysine chloromethylketone. Histidine 100-109 coagulation factor II, thrombin Homo sapiens 24-32 8381406-2 1993 An anilinonaphthalene-6-sulfonic acid (ANS) dye was attached covalently to the active site histidine of thrombin via a D-Phe-Pro-Arg (FPR) linkage to form ANS-FPR-thrombin. Histidine 91-100 coagulation factor II, thrombin Homo sapiens 104-112 8381406-2 1993 An anilinonaphthalene-6-sulfonic acid (ANS) dye was attached covalently to the active site histidine of thrombin via a D-Phe-Pro-Arg (FPR) linkage to form ANS-FPR-thrombin. Histidine 91-100 coagulation factor II, thrombin Homo sapiens 163-171 2147282-4 1990 Site-directed mutagenesis of the His-53, Asp-77, and Ser-138 residues of NS3 that compose the proposed catalytic triad implicates this domain as a serine protease. Histidine 33-36 coagulation factor II, thrombin Homo sapiens 147-162 2847349-3 1988 Modification of thrombin at the catalytic site serine and histidine residues, with Diisopropylfluorophosphate and Tosyl-L-lysine chloromethyl ketone, resulted in loss of clotting and amidolytic activity. Histidine 58-67 coagulation factor II, thrombin Homo sapiens 16-24 3456154-9 1986 It is of interest that histidine-16 of the B beta chain, which is only one residue away from the thrombin-susceptible bond, makes a part of the site A for the end-to-end association created by the release of fibrinopeptide A. Histidine 23-32 coagulation factor II, thrombin Homo sapiens 97-105 1421398-0 1992 Prothrombin Himi: a compound heterozygote for two dysfunctional prothrombin molecules (Met-337-->Thr and Arg-388-->His). Histidine 121-124 coagulation factor II, thrombin Homo sapiens 0-11 1421398-0 1992 Prothrombin Himi: a compound heterozygote for two dysfunctional prothrombin molecules (Met-337-->Thr and Arg-388-->His). Histidine 121-124 coagulation factor II, thrombin Homo sapiens 64-75 1634535-7 1992 The incorporated label was localized to a 9000 molecular weight region of alpha and beta/gamma-thrombin containing the catalytic-site histidine residue. Histidine 134-143 coagulation factor II, thrombin Homo sapiens 95-103 2059622-1 1991 A mutant of the serine protease, subtilisin BPN", in which the catalytic His64 is replaced by Ala (H64A), is very specific for substrates containing a histidine, presumably by the substrate-bound histidine assisting in catalysis [Carter, P., & Wells, J.A. Histidine 151-160 coagulation factor II, thrombin Homo sapiens 16-31 2059622-1 1991 A mutant of the serine protease, subtilisin BPN", in which the catalytic His64 is replaced by Ala (H64A), is very specific for substrates containing a histidine, presumably by the substrate-bound histidine assisting in catalysis [Carter, P., & Wells, J.A. Histidine 196-205 coagulation factor II, thrombin Homo sapiens 16-31 2499045-1 1989 The hydrogen-bonding status of His57 in the catalytic triad (Asp-His-Ser) of serine protease has important mechanistic implications for this class of enzymes. Histidine 31-34 coagulation factor II, thrombin Homo sapiens 77-92 3146347-2 1988 Since thrombin has a primary specificity for basic amino acids, each of the three basic residues and the histidine in hirudin were mutated to glutamine. Histidine 105-114 coagulation factor II, thrombin Homo sapiens 6-14 3663613-3 1987 Modification of the active-site histidine with D-Phe-Pro-Arg-CH2Cl resulted in a form of thrombin with a 10(6)-fold reduced affinity for hirudin. Histidine 32-41 coagulation factor II, thrombin Homo sapiens 89-97 3016716-10 1986 The peptide Tyr-His-His-Lys-Arg-Lys-Arg-Lys-Gln-Arg-Gly-Asp-Val was labeled with 125I to quantitate its binding to thrombin-stimulated platelets; at saturation, 59,990 molecules were bound per cell (Kd = 3.8 X 10(-7) M). Histidine 16-19 coagulation factor II, thrombin Homo sapiens 115-123 3016716-10 1986 The peptide Tyr-His-His-Lys-Arg-Lys-Arg-Lys-Gln-Arg-Gly-Asp-Val was labeled with 125I to quantitate its binding to thrombin-stimulated platelets; at saturation, 59,990 molecules were bound per cell (Kd = 3.8 X 10(-7) M). Histidine 20-23 coagulation factor II, thrombin Homo sapiens 115-123 3886645-8 1985 Statistical analysis of residual enzyme activity and extent of modification indicates that among 7 histidyl residues modified per molecule, there is 1 essential histidine (not in the active site) involved in the potential fibrinogen-clotting activity of prothrombin. Histidine 161-170 coagulation factor II, thrombin Homo sapiens 254-265 29717998-0 2018 In situ proteolysis of an N-terminal His tag with thrombin improves the diffraction quality of human aldo-keto reductase 1C3 crystals. Histidine 37-40 coagulation factor II, thrombin Homo sapiens 50-58 47704-18 1975 Antithrombin III neutralizes the activity of prethrombin-E and thrombin-E; consequently, an active histidine center found in the B1 chain of thrombin is not essential for the binding of antithrombin. Histidine 99-108 coagulation factor II, thrombin Homo sapiens 4-12 31957446-9 2020 The thrombin-cleaved PAR4 exhibited a 2-fold increase (p > 0.01) in t1/2 values observed for four histidine residues (His180, His229, His240, and His380), demonstrating that these regions have decreased solvent accessibility upon thrombin treatment. Histidine 98-107 coagulation factor II, thrombin Homo sapiens 4-12 6523446-10 1984 The ionization of this histidine residue and the accompanying conformational changes could explain the reduced catalytic efficiency and stability of alpha-thrombin at pH 6. Histidine 23-32 coagulation factor II, thrombin Homo sapiens 155-163 33260771-11 2020 LIC11112, LIC20143, and LIC11037 have the serine protease domain with the conserved catalytic triad His-Asp-Ser. Histidine 100-103 coagulation factor II, thrombin Homo sapiens 42-57 26161660-1 2015 Herpesviruses encode a characteristic serine protease with a unique fold and an active site that comprises the unusual triad Ser-His-His. Histidine 129-132 coagulation factor II, thrombin Homo sapiens 38-53 25172132-9 2014 We show that the thrombin-mediated cleavage of two histidine tags from the purified recombinant protein and the adsorption of these histidine tags and their associated endotoxins to a nickel affinity column result in an appreciable depletion of the endotoxins in the purified protein fraction. Histidine 51-60 coagulation factor II, thrombin Homo sapiens 17-25 25671465-3 2015 Reduction of the Co(III)-sb by PET initiates displacement of the inert axial imidazole ligands, promoting coordination to active site histidines of alpha-thrombin. Histidine 134-144 coagulation factor II, thrombin Homo sapiens 154-162 25172132-9 2014 We show that the thrombin-mediated cleavage of two histidine tags from the purified recombinant protein and the adsorption of these histidine tags and their associated endotoxins to a nickel affinity column result in an appreciable depletion of the endotoxins in the purified protein fraction. Histidine 132-141 coagulation factor II, thrombin Homo sapiens 17-25 24148431-3 2013 Thrombin can selectively digest EGFP accompanied by His-tag peptide sequence leaving, and the resulting EGFP cannot be captured by Ni(2+)-NTA MNPs and kept in supernatant. Histidine 52-55 coagulation factor II, thrombin Homo sapiens 0-8 24878753-0 2014 Optimization of expression of untagged and histidine-tagged human recombinant thrombin precursors in Escherichia coli. Histidine 43-52 coagulation factor II, thrombin Homo sapiens 78-86 23195954-2 2013 We demonstrate unknown facets of calnuc, which is a serine protease in which Ser-378 of GXSXG motif, Asp-328 of DTG motif, and His-339 form the "catalytic triad," locating the enzyme active site in the C-terminal region. Histidine 127-130 coagulation factor II, thrombin Homo sapiens 52-67 22959014-3 2013 Then, the histidine labeled thrombin aptamer was immobilized onto the electrode through coordination of the histidine groups on the NTA-Cu(2+) complex. Histidine 10-19 coagulation factor II, thrombin Homo sapiens 28-36 22959014-3 2013 Then, the histidine labeled thrombin aptamer was immobilized onto the electrode through coordination of the histidine groups on the NTA-Cu(2+) complex. Histidine 108-117 coagulation factor II, thrombin Homo sapiens 28-36 21116528-0 2011 Serine protease acylation proceeds with a subtle re-orientation of the histidine ring at the tetrahedral intermediate. Histidine 71-80 coagulation factor II, thrombin Homo sapiens 0-15 22178916-0 2012 Microplate assay for aptamer-based thrombin detection using a DNA-enzyme conjugate based on histidine-tag chemistry. Histidine 92-101 coagulation factor II, thrombin Homo sapiens 35-43 23228830-8 2012 RESULTS: Recombinant proteins and their respective products whose N-terminal his-tag were removed with thrombin were recognized by serum from the patient infected with H. pylori. Histidine 77-80 coagulation factor II, thrombin Homo sapiens 103-111 16973378-3 2007 The histidine tag was removed from the human enzyme by thrombin digestion and the adenylosuccinate lyase was purified by Sephadex G-100 gel filtration. Histidine 4-13 coagulation factor II, thrombin Homo sapiens 55-63 16055450-1 2005 Six Cys(2)His(2) zinc fingers (F1-6) comprise the DNA binding domain of metal-responsive element binding transcription factor-1 (MTF-1). Histidine 10-13 coagulation factor II, thrombin Homo sapiens 31-35 16797105-6 2006 Inhibition of thrombin, amounting to a 63.3% and 36.7% reduction in the rate of fibrin formation, was noted for cyclo(His-Ala) and cyclo(His-Gly), respectively. Histidine 118-121 coagulation factor II, thrombin Homo sapiens 14-22 16797105-6 2006 Inhibition of thrombin, amounting to a 63.3% and 36.7% reduction in the rate of fibrin formation, was noted for cyclo(His-Ala) and cyclo(His-Gly), respectively. Histidine 137-140 coagulation factor II, thrombin Homo sapiens 14-22 17001711-1 2006 In the completion of our fluorine scan of tricyclic inhibitors to map the fluorophilicity/fluorophobicity of the thrombin active site, a series of 11 new ligands featuring alkyl, alkenyl, and fluoroalkyl groups was prepared to explore fluorine effects on binding into the hydrophobic proximal (P) pocket, lined by Tyr 60A and Trp 60D, His 57, and Leu 99. Histidine 335-338 coagulation factor II, thrombin Homo sapiens 113-121 12794066-6 2003 Surface residues of thrombin further involved in high specificity fibrin-enhanced factor XIII activation were identified as His-66, Tyr-71, and Asn-74. Histidine 124-127 coagulation factor II, thrombin Homo sapiens 20-28 15771442-19 2005 The X-ray crystal structures of the ternary complexes 3-thrombin-hirugen and 4-thrombin-hirugen depict novel interactions in the S(1)" region, with the benzothiazole ring forming a hydrogen bond with His-57 and an aromatic stacking interaction with Trp-60D of thrombin"s insertion loop. Histidine 200-203 coagulation factor II, thrombin Homo sapiens 56-64 15771442-19 2005 The X-ray crystal structures of the ternary complexes 3-thrombin-hirugen and 4-thrombin-hirugen depict novel interactions in the S(1)" region, with the benzothiazole ring forming a hydrogen bond with His-57 and an aromatic stacking interaction with Trp-60D of thrombin"s insertion loop. Histidine 200-203 coagulation factor II, thrombin Homo sapiens 79-87 15771442-19 2005 The X-ray crystal structures of the ternary complexes 3-thrombin-hirugen and 4-thrombin-hirugen depict novel interactions in the S(1)" region, with the benzothiazole ring forming a hydrogen bond with His-57 and an aromatic stacking interaction with Trp-60D of thrombin"s insertion loop. Histidine 200-203 coagulation factor II, thrombin Homo sapiens 79-87 15498522-13 2004 We also identified another serine protease fragment that has only the conserved histidine residue. Histidine 80-89 coagulation factor II, thrombin Homo sapiens 27-42 15735791-3 2005 Due to the specificity of an inhibitor being influenced by the amino acid residue at the P1 position, we replaced the Arg10 at P1 position of dipetarudin by a histidine, which is the P1 residue of rhodniin, a very specific thrombin inhibitor. Histidine 159-168 coagulation factor II, thrombin Homo sapiens 223-231 12926277-1 2003 The synthesis of a Tentagel-supported peptide incorporating the reactive triad of serine, histidine and aspartic acid, found within serine protease enzymes, is described. Histidine 90-99 coagulation factor II, thrombin Homo sapiens 132-147 12702176-9 2003 l-Histidine added to plasma before MB plus red light treatment normalized the thrombin-induced coagulation time in a dose-dependent way. Histidine 0-11 coagulation factor II, thrombin Homo sapiens 78-86 12706644-1 2003 INTRODUCTION: The exchange of Aalpha 16, Arg for Cys or His is the most common molecular defect in dysfibrinogenemia directly affecting the thrombin cleavage site involved in fibrinopeptide A (FPA) release. Histidine 56-59 coagulation factor II, thrombin Homo sapiens 140-148