PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17081490-9 2006 While the successful incorporation of the His-tag into our constructs was confirmed by Epo binding to Ni(2+)- nitrilotriacetic acid resin and by microcapillary reverse-phase high-performance liquid chromatography nano-electrospray tandem mass spectrometery amino acid sequencing, the levels of immunodetection of His-tagged protein varied markedly depending on the particular anti-His-tag antibody used. Histidine 42-45 erythropoietin Cricetulus griseus 87-90