PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11319928-5 2001 The large putative Rep protein encoded by pME2001 was overexpressed in Escherichia coli as an N-terminal His-tagged version using pET28a and a compatible helper plasmid that coexpresses minor tRNAs, argU and ileX to compensate for codon usage difference. Histidine 105-108 replication protein Escherichia coli 19-22