PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2780051-7 1989 Furthermore, amplification of neu and the epidermal growth factor receptor gene could be detected in as few as 100 breast carcinoma cells or in single sections of formalin-fixed, embedded material. Formaldehyde 163-171 erb-b2 receptor tyrosine kinase 2 Homo sapiens 30-33 2903446-3 1988 We describe a method for the immunohistochemical detection of overexpression of neu protein on formalin-fixed paraffin-embedded tissue, with the use of two different monoclonal antibodies. Formaldehyde 95-103 erb-b2 receptor tyrosine kinase 2 Homo sapiens 80-83 33893247-6 2021 The accuracy of ERBB2 copy number variations in tissue/formalin-fixed and paraffin-embedded samples was 95% with 86% sensitivity and 99% specificity. Formaldehyde 55-63 erb-b2 receptor tyrosine kinase 2 Homo sapiens 16-21 33280268-1 2021 BACKGROUND: Human epidermal growth factor receptor 2-in situ hybridization (HER2-ISH) is widely approved for diagnostic, prognostic biomarker testing of formalin-fixed paraffin-embedded tissue blocks. Formaldehyde 153-161 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-52 33280268-1 2021 BACKGROUND: Human epidermal growth factor receptor 2-in situ hybridization (HER2-ISH) is widely approved for diagnostic, prognostic biomarker testing of formalin-fixed paraffin-embedded tissue blocks. Formaldehyde 153-161 erb-b2 receptor tyrosine kinase 2 Homo sapiens 76-80 33198632-4 2020 HER2 gene CNV was also assessed in formalin-fixed paraffin-embedded (FFPE) tumor tissue. Formaldehyde 35-43 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 32860265-3 2020 The GPA was evaluated using 67 formalin-fixed paraffin-embedded (FFPE) HER2 equivoval IHC (2+) breast cancer tissue samples. Formaldehyde 31-39 erb-b2 receptor tyrosine kinase 2 Homo sapiens 71-75 33001186-6 2020 METHODS: We quantified HER2 expression levels in 210 breast cancer formalin-fixed paraffin-embedded (FFPE) tissue samples by MRM-MS. We aimed to improve the accuracy and precision of HER2 quantification by simplifying the sample preparation through predicting the number of FFPE slides required to ensure an adequate amount of protein and using the expression levels of an epithelial cell-specific protein as a normalization factor when measuring HER2 expression levels. Formaldehyde 67-75 erb-b2 receptor tyrosine kinase 2 Homo sapiens 23-27 32621174-4 2020 We developed an amplicon-based panel to detect human epidermal receptor growth factor (HER2) amplification in formalin-fixed paraffin-embedded (FFPE) tumor tissue samples from 280 breast cancer and 50 gastric cancer patients. Formaldehyde 110-118 erb-b2 receptor tyrosine kinase 2 Homo sapiens 87-91 32674482-3 2020 We analyzed ERBB2 expression using a clinically applicable assay in formalin-fixed paraffin-embedded (FFPE) tumors (primary or metastatic) from a retrospective series of 77 patients with advanced HER2+ BC treated with T-DM1. Formaldehyde 68-76 erb-b2 receptor tyrosine kinase 2 Homo sapiens 12-17 31796633-2 2020 METHODS: We evaluated the characteristics of HER2-positivity pattern in formalin-fixed paraffin-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Formaldehyde 72-80 erb-b2 receptor tyrosine kinase 2 Homo sapiens 45-49 31205070-3 2020 We investigated 6 antibodies that are commonly used for breast cancer subtyping in research studies with immunohistochemistry (ER, PR, HER2, CK5/6, EGFR, and Ki67) in formalin-fixed paraffin-embedded breast tissue microarrays consisting of 148 patients. Formaldehyde 167-175 erb-b2 receptor tyrosine kinase 2 Homo sapiens 135-139 32110671-5 2020 Next-generation sequencing on formalin fixed paraffin-embedded (FFPE) tissue was also performed and suggested HER2 gene amplification in the FFPE tissue. Formaldehyde 30-38 erb-b2 receptor tyrosine kinase 2 Homo sapiens 110-114 31638234-0 2019 Development of recurrence risk score using 95-gene classifier and its application to formalin-fixed paraffin-embedded tissues in ER-positive, HER2-negative and node-negative breast cancer. Formaldehyde 85-93 erb-b2 receptor tyrosine kinase 2 Homo sapiens 142-146 31759380-6 2019 METHODS: Immunohistochemical analysis was performed to evaluate the expression of COX-2, IDO, estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) on formalin-fixed paraffin-embedded breast cancer tissues of 100 patients. Formaldehyde 200-208 erb-b2 receptor tyrosine kinase 2 Homo sapiens 155-189 31759380-6 2019 METHODS: Immunohistochemical analysis was performed to evaluate the expression of COX-2, IDO, estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) on formalin-fixed paraffin-embedded breast cancer tissues of 100 patients. Formaldehyde 200-208 erb-b2 receptor tyrosine kinase 2 Homo sapiens 191-195 31544361-3 2019 In the current study, the authors evaluated the concordance of HER2 status between bright-field dual in situ hybridization (DISH), fluorescence in situ hybridization (FISH), and HER2 immunohistochemistry (IHC) performed on formalin-fixed CBs of recurrent and metastatic breast cancers. Formaldehyde 223-231 erb-b2 receptor tyrosine kinase 2 Homo sapiens 63-67 31544361-12 2019 DISH can be used in tandem with IHC as a reflex assay instead of FISH and is an efficient and reliable method with which to determine HER2 amplification in formalin-fixed CBs. Formaldehyde 156-164 erb-b2 receptor tyrosine kinase 2 Homo sapiens 134-138 31455202-0 2019 Visualisation of HER2 homodimers in single cells from HER2 overexpressing primary formalin fixed paraffin embedded tumour tissue. Formaldehyde 82-90 erb-b2 receptor tyrosine kinase 2 Homo sapiens 17-21 31455202-0 2019 Visualisation of HER2 homodimers in single cells from HER2 overexpressing primary formalin fixed paraffin embedded tumour tissue. Formaldehyde 82-90 erb-b2 receptor tyrosine kinase 2 Homo sapiens 54-58 31401730-6 2019 qPCR was performed for the HER2 gene and amyloid precursor protein (reference gene) in formalin-fixed paraffin-embedded GCa tissue. Formaldehyde 87-95 erb-b2 receptor tyrosine kinase 2 Homo sapiens 27-31 31197538-7 2019 It was then extended to image and quantitatively evaluate HER2 homodimers in clinical formalin-fixed paraffin-embedded breast cancer tissue specimens. Formaldehyde 86-94 erb-b2 receptor tyrosine kinase 2 Homo sapiens 58-62 30882204-6 2019 We used the ImCal method to quantify HER2 in 35 breast cancer formalin-fixed paraffin-embedded patient samples, revealing a high degree of heterogeneity among patients, which contrasts with the homogeneous immunohistochemistry patient classification. Formaldehyde 62-70 erb-b2 receptor tyrosine kinase 2 Homo sapiens 37-41 30842953-0 2019 Formalin fixation on HER-2 and PD-L1 expression in gastric cancer: A pilot analysis using the same surgical specimens with different fixation times. Formaldehyde 0-8 erb-b2 receptor tyrosine kinase 2 Homo sapiens 21-26 30838039-6 2019 Results: The concordance rate of HER2 amplification examined in plasma and formalin-fixed paraffin-embedded (FFPE) samples with ddPCR was 81.4%, with a sensitivity of 76.5% and a specificity of 83.8%. Formaldehyde 75-83 erb-b2 receptor tyrosine kinase 2 Homo sapiens 33-37 30563489-0 2018 A novel detection methodology for HER2 protein quantitation in formalin-fixed, paraffin embedded clinical samples using fluorescent nanoparticles: an analytical and clinical validation study. Formaldehyde 63-71 erb-b2 receptor tyrosine kinase 2 Homo sapiens 34-38 30521571-2 2018 Formalin-fixed paraffin-embedded tumor tissue samples were collected from 227 patients, considered to be HER2-positive when assessed at the local laboratories. Formaldehyde 0-8 erb-b2 receptor tyrosine kinase 2 Homo sapiens 105-109 30370669-5 2018 The authors also analyze the participation of oncoproteins such as fibulins, FGFR1, HER2/neu, FBI-1, and MUC1-C in the control of genes related to formaldehyde metabolism, suggesting the existence of two mutually exclusive processes in cancer cells: 1) production and 2) oxidation and elimination of formaldehyde from the cell. Formaldehyde 147-159 erb-b2 receptor tyrosine kinase 2 Homo sapiens 84-88 30370669-5 2018 The authors also analyze the participation of oncoproteins such as fibulins, FGFR1, HER2/neu, FBI-1, and MUC1-C in the control of genes related to formaldehyde metabolism, suggesting the existence of two mutually exclusive processes in cancer cells: 1) production and 2) oxidation and elimination of formaldehyde from the cell. Formaldehyde 147-159 erb-b2 receptor tyrosine kinase 2 Homo sapiens 89-92 30391779-8 2018 We found that the use of fixatives other than 10% neutral buffered formalin significantly reduced the HER2-positive rate. Formaldehyde 67-75 erb-b2 receptor tyrosine kinase 2 Homo sapiens 102-106 29866615-3 2018 HER2 immunohistochemistry was performed using formalin-fixed, paraffin-embedded primary tumor specimens. Formaldehyde 46-54 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 30087576-2 2018 The present study was designed to evaluate a chip-based digital PCR (dPCR) system for assessing HER2 amplification from formalin-fixed paraffin-embedded breast carcinoma tissue and to compare this system with immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Formaldehyde 120-128 erb-b2 receptor tyrosine kinase 2 Homo sapiens 96-100 30087576-6 2018 Conclusion: These results demonstrate that chip-based dPCR is suitable for HER2 amplification detection in formalin-fixed paraffin-embedded samples in a clinical setting, providing the advantages of superior turnaround time, cost-effectiveness, and increased precision with absolute quantification compared with conventional tests such as FISH and IHC. Formaldehyde 107-115 erb-b2 receptor tyrosine kinase 2 Homo sapiens 75-79 29661737-1 2018 Prolonged time from specimen excision to adequate formalin exposure, or cold ischemic time (CIT), negatively impacts estrogen receptor (ER), progesterone receptor (PR) and HER-2 biomarker studies routinely performed on breast specimens. Formaldehyde 50-58 erb-b2 receptor tyrosine kinase 2 Homo sapiens 172-177 29471352-1 2018 Objectives: The gold standard of examining breast biomarkers, including estrogen receptor (ER)/progesterone receptor (PR)/human epidermal growth factor receptor 2 (HER2)/Ki-67, is to perform these assays on formalin-fixed, paraffin-embedded tissue. Formaldehyde 207-215 erb-b2 receptor tyrosine kinase 2 Homo sapiens 128-162 29364207-0 2017 Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation. Formaldehyde 50-58 erb-b2 receptor tyrosine kinase 2 Homo sapiens 13-17 29364207-0 2017 Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation. Formaldehyde 127-135 erb-b2 receptor tyrosine kinase 2 Homo sapiens 13-17 29256235-0 2017 [Volume of 4% neutral buffered formaldehyde affects the results of HER2 gene detected by fluorescence in situ hybridization in primary invasive breast cancer]. Formaldehyde 31-43 erb-b2 receptor tyrosine kinase 2 Homo sapiens 67-71 28039034-3 2017 In the current study, we explored the use of quantitative real time reverse transcription-PCR (RT-qPCR) as a potential method for the accurate relative quantification of the HER2 gene using formalin fixed paraffin embedded (FFPE) breast cancer biopsy samples. Formaldehyde 190-198 erb-b2 receptor tyrosine kinase 2 Homo sapiens 174-178 28039535-4 2017 METHODS: In this study, we used ddPCR to measure the HER2 gene copy number in clinical formalin-fixed paraffin-embedded samples of 41 primary breast cancer patients. Formaldehyde 87-95 erb-b2 receptor tyrosine kinase 2 Homo sapiens 53-57 27687309-5 2017 We applied mass spectrometry-based proteomic analysis to quantify HER2 protein expression in formalin-fixed tumor samples. Formaldehyde 93-101 erb-b2 receptor tyrosine kinase 2 Homo sapiens 66-70 28901323-0 2017 Human epidermal growth factor receptor 2 amplification detection by droplet digital polymerase chain reaction in formalin-fixed paraffin-embedded breast and gastric cancer samples. Formaldehyde 113-121 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-40 27544315-0 2017 Clinical Usefulness of a One-Tube Nested Reverse Transcription Quantitative Polymerase Chain Reaction Assay for Evaluating Human Epidermal Growth Factor Receptor 2 mRNA Overexpression in Formalin-Fixed and Paraffin-Embedded Breast Cancer Tissue Samples. Formaldehyde 187-195 erb-b2 receptor tyrosine kinase 2 Homo sapiens 123-163 27544315-3 2017 METHODS: In this study, we analyzed HER2 mRNA expression in clinical formalin-fixed and paraffin-embedded (FFPE) samples using a one-tube nested reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay. Formaldehyde 69-77 erb-b2 receptor tyrosine kinase 2 Homo sapiens 36-40 27929428-4 2016 By immunohistochemistry and fluorescence in-situ hybridization, HER2 expression was investigated in formalin-fixed paraffin-embedded surgical specimens from 90 DTC patients, 45 follicular (FTC) and 45 papillary (PTC) histotypes. Formaldehyde 100-108 erb-b2 receptor tyrosine kinase 2 Homo sapiens 64-68 26746842-9 2016 CONCLUSION: The HER2 DISH assay, utilizing 10 % buffered formalin-fixed CB, would be a reliable and ideal method to assess the HER2 gene status of breast cancer cytological specimens. Formaldehyde 57-65 erb-b2 receptor tyrosine kinase 2 Homo sapiens 16-20 26746842-9 2016 CONCLUSION: The HER2 DISH assay, utilizing 10 % buffered formalin-fixed CB, would be a reliable and ideal method to assess the HER2 gene status of breast cancer cytological specimens. Formaldehyde 57-65 erb-b2 receptor tyrosine kinase 2 Homo sapiens 127-131 27315045-10 2016 CONCLUSIONS: ER, PR, and HER2 determination on FNA-acquired cell block (fixed exclusively in 10% formalin) showed excellent agreement for ER and HER2 and moderate agreement for PR with the corresponding tissue block. Formaldehyde 97-105 erb-b2 receptor tyrosine kinase 2 Homo sapiens 25-29 27315045-10 2016 CONCLUSIONS: ER, PR, and HER2 determination on FNA-acquired cell block (fixed exclusively in 10% formalin) showed excellent agreement for ER and HER2 and moderate agreement for PR with the corresponding tissue block. Formaldehyde 97-105 erb-b2 receptor tyrosine kinase 2 Homo sapiens 145-149 27559738-3 2016 Formalin-fixed paraffin-embedded tissue samples of esophageal biopsy specimens from 73 patients with Barrett"s esophagus with either low-grade dysplasia (LGD) (n = 32) or high-grade dysplasia (HGD) (n = 59) were analyzed for HER1, HER2, HER3 and CMET expression by immunohistochemistry (IHC). Formaldehyde 0-8 erb-b2 receptor tyrosine kinase 2 Homo sapiens 231-235 27618787-3 2016 The purpose of this study was to accurately quantify HER2-HER3 dimerisation in formalin fixed paraffin embedded (FFPE) breast cancer tissue as a novel prognostic biomarker.FFPE tissues were obtained from patients included in the METABRIC (Molecular Taxonomy of Breast Cancer International Consortium) study. Formaldehyde 79-87 erb-b2 receptor tyrosine kinase 2 Homo sapiens 53-57 26626802-0 2016 Droplet digital polymerase chain reaction detection of HER2 amplification in formalin fixed paraffin embedded breast and gastric carcinoma samples. Formaldehyde 77-85 erb-b2 receptor tyrosine kinase 2 Homo sapiens 55-59 27065677-5 2016 Immunohistochemistry (IHC) was carried out on all the cases to evaluate the expression of HER2 in formalin-fixed paraffin-embedded tissue samples. Formaldehyde 98-106 erb-b2 receptor tyrosine kinase 2 Homo sapiens 90-94 26820945-0 2016 Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH). Formaldehyde 97-105 erb-b2 receptor tyrosine kinase 2 Homo sapiens 14-54 26820945-0 2016 Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH). Formaldehyde 97-105 erb-b2 receptor tyrosine kinase 2 Homo sapiens 56-60 26422389-3 2016 METHODS: Using selected reaction monitoring mass spectrometry (SRM-MS), we quantified HER2 protein levels in formalin-fixed, paraffin-embedded (FFPE) tissue samples that had been classified as HER2 0, 1+, 2+ or 3+ by immunohistochemistry (IHC). Formaldehyde 109-117 erb-b2 receptor tyrosine kinase 2 Homo sapiens 86-90 26149442-0 2015 Quantification of HER2 by Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded (FFPE) Breast Cancer Tissues. Formaldehyde 56-64 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-22 26149442-2 2015 We have developed and evaluated a selectedreaction monitoring assay for the human receptor tyrosine-protein kinase erbB-2 (HER2) in formalin-fixed paraffin-embedded breast tumors. Formaldehyde 132-140 erb-b2 receptor tyrosine kinase 2 Homo sapiens 82-121 26149442-2 2015 We have developed and evaluated a selectedreaction monitoring assay for the human receptor tyrosine-protein kinase erbB-2 (HER2) in formalin-fixed paraffin-embedded breast tumors. Formaldehyde 132-140 erb-b2 receptor tyrosine kinase 2 Homo sapiens 123-127 26149442-6 2015 The six HER2 peptides were quantified by selected reaction monitoring in a cohort of 40 archival formalin-fixed paraffin-embedded tumor tissues from women with invasive breast carcinomas, which showed different levels of HER2 gene amplification as assessed by standard methods used in clinical pathology. Formaldehyde 97-105 erb-b2 receptor tyrosine kinase 2 Homo sapiens 8-12 26149442-7 2015 The amounts of the six HER2 peptides were highly and significantly correlated with each other, indicating that peptide levels can be used as surrogates of protein amounts in formalin-fixed paraffin-embedded tissues. Formaldehyde 174-182 erb-b2 receptor tyrosine kinase 2 Homo sapiens 23-27 25897674-2 2015 METHODS: We used droplet digital polymerase chain reaction (ddPCR) to evaluate HER2 amplification in formalin-fixed and paraffin-embedded (FFPE) samples and cell-free serum circulating tumour DNA (ctDNA) in 25 patients with gastric cancer. Formaldehyde 101-109 erb-b2 receptor tyrosine kinase 2 Homo sapiens 79-83 25236569-2 2014 In the present study, the BrightGen HER2 RT-qDx (Syantra, Calgary, Canada), which is based on a one-tube nested RT-qPCR method that detects HER2 mRNA overexpression, was clinically evaluated in a total of 237 formalin-fixed paraffin-embedded (FFPE) tissue samples from breast cancer patients. Formaldehyde 209-217 erb-b2 receptor tyrosine kinase 2 Homo sapiens 36-40 25279206-5 2014 HER-2 was detected by immunohistochemical examination on sections from formalin-fixed, paraffin-embedded tumor tissues. Formaldehyde 71-79 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-5 24992176-0 2014 Delay to formalin fixation effect on HER2 test in breast cancer by dual-color silver-enhanced in situ hybridization (Dual-ISH). Formaldehyde 9-17 erb-b2 receptor tyrosine kinase 2 Homo sapiens 37-41 24992176-2 2014 One of the variables that negatively affects HER2 test is delay to formalin fixation (DFF). Formaldehyde 67-75 erb-b2 receptor tyrosine kinase 2 Homo sapiens 45-49 25838812-0 2014 HER-2 assessment in formalin-fixed paraffin-embedded breast cancer tissue by well-based reverse phase protein array. Formaldehyde 20-28 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-5 25838812-6 2014 By well-based RPPA, we successfully detected HER-2 expression in 76 human breast formalin-fixed paraffin-embedded tissue samples. Formaldehyde 81-89 erb-b2 receptor tyrosine kinase 2 Homo sapiens 45-50 24414131-2 2014 Here we examined the impact of formalin fixing conditions on HER2 immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in xenografted tumor tissues. Formaldehyde 31-39 erb-b2 receptor tyrosine kinase 2 Homo sapiens 61-65 25218890-3 2014 EndoPredict is a quantitative reverse transcription PCR assay conducted on RNA from formalin-fixed, paraffin-embedded (FFPE) tissue that predicts the likelihood of distant recurrence in patients with ER-positive/HER2-negative breast cancer. Formaldehyde 84-92 erb-b2 receptor tyrosine kinase 2 Homo sapiens 212-216 25400755-0 2014 Quantitative RT-PCR assay of HER2 mRNA expression in formalin-fixed and paraffin-embedded breast cancer tissues. Formaldehyde 53-61 erb-b2 receptor tyrosine kinase 2 Homo sapiens 29-33 25400755-6 2014 Here we employed a quantitative PCR method to detect HER2 expression in one hundred ninety nine formalin-fixed and paraffin-embedded (FFPE) breast cancer tissue samples from the patients treated over two years at the Yonsei University Severance Hospital, Republic of Korea. Formaldehyde 96-104 erb-b2 receptor tyrosine kinase 2 Homo sapiens 53-57 24926080-0 2014 The effects of under 6 hours of formalin fixation on hormone receptor and HER2 expression in invasive breast cancer: a systematic review. Formaldehyde 32-40 erb-b2 receptor tyrosine kinase 2 Homo sapiens 74-78 24491355-4 2014 A combined analysis of immunohistochemistry and fluorescence in situ hybridization revealed ERBB2-amplified cancer cells in 51 of 475 formalin-fixed, paraffin-embedded gastric adenocarcinomas. Formaldehyde 134-142 erb-b2 receptor tyrosine kinase 2 Homo sapiens 92-97 24057116-7 2014 Knockdown of ErbB2 relieves the aggravation of NRG-1 on formalin-induced neuropathic pain, and knockdown of ErbB4 could relieve the inhibition of NRG-1 on neuropathic pain in the SNI model. Formaldehyde 56-64 erb-b2 receptor tyrosine kinase 2 Homo sapiens 13-18 24529991-5 2014 We show that DBs specifically detect the activated HER2/HER3 complex in formalin-fixed, paraffin-embedded cancer cells and exhibit superior detection specificity for phospho-HER3 compared to the corresponding monoclonal antibody. Formaldehyde 72-80 erb-b2 receptor tyrosine kinase 2 Homo sapiens 51-55 23702645-4 2014 METHODS: HER2 protein expression was investigated using a FDA-approved antibody in 87 formalin-fixed and paraffin-embedded cases of PDAC with complete follow-up. Formaldehyde 86-94 erb-b2 receptor tyrosine kinase 2 Homo sapiens 9-13 24100522-0 2013 Reliable PCR quantitation of estrogen, progesterone and ERBB2 receptor mRNA from formalin-fixed, paraffin-embedded tissue is independent of prior macro-dissection. Formaldehyde 81-89 erb-b2 receptor tyrosine kinase 2 Homo sapiens 56-61 24278211-10 2013 CONCLUSIONS: The data suggest that measurement of both HER2 and phospho- Tyr(1248)HER2, in formalin-fixed tissue by immunological methods is significantly affected by pre-analytic variables. Formaldehyde 91-99 erb-b2 receptor tyrosine kinase 2 Homo sapiens 55-59 24278211-10 2013 CONCLUSIONS: The data suggest that measurement of both HER2 and phospho- Tyr(1248)HER2, in formalin-fixed tissue by immunological methods is significantly affected by pre-analytic variables. Formaldehyde 91-99 erb-b2 receptor tyrosine kinase 2 Homo sapiens 82-86 23447357-12 2013 In summary, formalin fixation from 2 to 96 hours and preparation of cells as cell blocks produces optimal results for ER, PR, and HER-2 testing in human breast cancer cells. Formaldehyde 12-20 erb-b2 receptor tyrosine kinase 2 Homo sapiens 130-135 23727348-0 2013 Detection of truncated HER2 forms in formalin-fixed, paraffin-embedded breast cancer tissue captures heterogeneity and is not affected by HER2-targeted therapy. Formaldehyde 37-45 erb-b2 receptor tyrosine kinase 2 Homo sapiens 23-27 22807465-1 2013 The use of formalin-fixed cell blocks (CBs) for detection of ER, PR, and Her2 status of primary and metastatic breast carcinomas sampled by fine-needle aspiration (FNA) has been extensively used in clinical practice; however, CBs sometimes lack adequate cellularity even when direct smears are cellular. Formaldehyde 11-19 erb-b2 receptor tyrosine kinase 2 Homo sapiens 73-77 23358413-0 2013 Droplet digital PCR measurement of HER2 copy number alteration in formalin-fixed paraffin-embedded breast carcinoma tissue. Formaldehyde 66-74 erb-b2 receptor tyrosine kinase 2 Homo sapiens 35-39 23084583-2 2013 In the present study, we evaluated HER2 mRNA expression using a new mRNA in situ detection technique called RNAscope in 211 cases of formalin-fixed, paraffin-embedded gastric carcinoma. Formaldehyde 133-141 erb-b2 receptor tyrosine kinase 2 Homo sapiens 35-39 22611048-4 2013 The aim of this study was to evaluate and to compare ER, PR and Her2 IHC results on formalin-fixed CB sections to those observed on subsequent surgical (needle core or resection) specimens (SS). Formaldehyde 84-92 erb-b2 receptor tyrosine kinase 2 Homo sapiens 64-68 23391338-3 2013 METHODS: HER2 levels were centrally analyzed by immunohistochemistry (IHC), silver in situ hybridization (SISH) and qRT-PCR in 217 pretherapeutic formalin-fixed, paraffin-embedded (FFPE) core biopsies. Formaldehyde 146-154 erb-b2 receptor tyrosine kinase 2 Homo sapiens 9-13 23036330-8 2013 Here we present a study which investigates whether ddPCR can be used to assess HER2 transcript levels in formalin-fixed paraffin embedded (FFPE) human breast tumors and whether these ddPCR measurements agree with prior assessments of these same samples by pathologists using immunohistochemistry (IHC) and in some cases fluorescence in situ hybridization (FISH). Formaldehyde 105-113 erb-b2 receptor tyrosine kinase 2 Homo sapiens 79-83 23030524-0 2012 Assessing HER2 amplification by IHC, FISH, and real-time polymerase chain reaction analysis (real-time PCR) following LCM in formalin-fixed paraffin embedded tissue from 40 women with ovarian cancer. Formaldehyde 125-133 erb-b2 receptor tyrosine kinase 2 Homo sapiens 10-14 22814649-7 2012 Pronounced staining was possible for EGFR and IGF-1R with all alternative fixatives but HOPE , and phospho-HER2 staining was only noteworthy with formalin-ultrasound-fixed tissue. Formaldehyde 146-154 erb-b2 receptor tyrosine kinase 2 Homo sapiens 107-111 22417857-1 2012 The study of proto-oncogene Her-2/neu using the fluorescence in situ hybridization (FISH) technique in routinely paraffin-embedded formalin-fixed tissue has become commonplace over the past decade and mandatory among invasive breast cancer expressing a score 2+ by immunohistochemical analysis of c-erbB2 protein. Formaldehyde 131-139 erb-b2 receptor tyrosine kinase 2 Homo sapiens 34-37 22417858-4 2012 HER2 expression was detected by immunohistochemistry (IHC) performed on formalin-fixed, paraffin-embedded sections of the corresponding tumors. Formaldehyde 72-80 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 22647525-0 2012 A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections. Formaldehyde 181-189 erb-b2 receptor tyrosine kinase 2 Homo sapiens 25-65 22647525-0 2012 A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections. Formaldehyde 181-189 erb-b2 receptor tyrosine kinase 2 Homo sapiens 67-71 22647525-0 2012 A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections. Formaldehyde 181-189 erb-b2 receptor tyrosine kinase 2 Homo sapiens 112-116 22647525-0 2012 A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections. Formaldehyde 181-189 erb-b2 receptor tyrosine kinase 2 Homo sapiens 112-116 22647525-3 2012 METHODS: The HER2 gene-protein assay was optimized using formalin-fixed, paraffin-embedded (FFPE) samples of the xenograft tumors MCF7 [HER2 negative (non-amplified gene, protein negative)] and Calu-3 [HER2 positive (amplified gene, protein positive)]. Formaldehyde 57-65 erb-b2 receptor tyrosine kinase 2 Homo sapiens 13-17 22523206-0 2012 The effect of 96-hour formalin fixation on the immunohistochemical evaluation of estrogen receptor, progesterone receptor, and HER2 expression in invasive breast carcinoma. Formaldehyde 22-30 erb-b2 receptor tyrosine kinase 2 Homo sapiens 127-131 22261801-8 2012 We identify multiple differentially methylated regions, including one in ERBB2 and one in ZEB2, whose methylation status is strongly associated with an epithelial phenotype in NSCLC cell lines, surgically resected tumors, and formalin-fixed biopsies from patients with NSCLC who went on to fail front-line chemotherapy. Formaldehyde 226-234 erb-b2 receptor tyrosine kinase 2 Homo sapiens 73-78 21391216-0 2012 Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR. Formaldehyde 33-41 erb-b2 receptor tyrosine kinase 2 Homo sapiens 125-129 22234627-5 2012 HER-2 protein was quantified as total HER-2 protein expression (H2T) by the HERmark assay (Monogram Biosciences, Inc., South San Francisco, CA) in formalin-fixed, paraffin-embedded tumor samples. Formaldehyde 148-156 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-5 22234627-5 2012 HER-2 protein was quantified as total HER-2 protein expression (H2T) by the HERmark assay (Monogram Biosciences, Inc., South San Francisco, CA) in formalin-fixed, paraffin-embedded tumor samples. Formaldehyde 148-156 erb-b2 receptor tyrosine kinase 2 Homo sapiens 38-43 21289364-2 2011 PATIENTS AND METHODS: Using a novel assay, HER2 protein expression (H2T) was measured in formalin-fixed, paraffin-embedded primary breast tumors from 98 women treated with trastuzumab-based therapy for MBC. Formaldehyde 89-97 erb-b2 receptor tyrosine kinase 2 Homo sapiens 43-47 22037080-2 2011 MATERIALS AND METHODS: Immunohistochemical staining for Her-2/neu was performed on 10% formalin-fixed, paraffin-embedded primary carcinoma of the breast from 83 patients, between 2003 and 2007 using anti-Her-2/neu rabbit polyclonal antibody (DakoCytomation, CA, USA) and reactivity detected by an avidin-biotin immunoperoxidase method. Formaldehyde 87-95 erb-b2 receptor tyrosine kinase 2 Homo sapiens 56-65 21488175-9 2011 In conclusion, IHC for Her2/neu, ER and PR performed on formalin-fixed, paraffin-embedded cell blocks prepared from fresh FNA and serous fluid is reliable in predicting the expression of these markers when correlated with IHC and FISH performed on the corresponding tumor tissue. Formaldehyde 56-64 erb-b2 receptor tyrosine kinase 2 Homo sapiens 23-31 21326033-0 2011 Quantitative determination of estrogen receptor, progesterone receptor, and HER2 mRNA in formalin-fixed paraffin-embedded tissue--a new option for predictive biomarker assessment in breast cancer. Formaldehyde 89-97 erb-b2 receptor tyrosine kinase 2 Homo sapiens 76-80 20661132-7 2010 The implementation of the modified ASCO/CAP and UK HER2 IHC scoring guidelines has minimal effect on either assay interpretation, showing that Oracle can be used as a methodology for accurately determining HER2 IHC status in formalin fixed, paraffin-embedded breast cancer tissue. Formaldehyde 225-233 erb-b2 receptor tyrosine kinase 2 Homo sapiens 206-210 20959666-1 2010 We previously reported that delayed formalin fixation (DFF) has a negative effect on immunohistochemical staining of estrogen receptor (ER), progesterone receptor (PR), and HER2. Formaldehyde 36-44 erb-b2 receptor tyrosine kinase 2 Homo sapiens 173-177 20855640-2 2010 The purpose of this pilot study was to determine the impact of the length of fixation in 10% buffered formalin on the expression of HER2/neu by immunohistochemical analysis. Formaldehyde 102-110 erb-b2 receptor tyrosine kinase 2 Homo sapiens 132-136 20740285-8 2010 For example, HER2 gene amplification is directly linked to the protein expression level in breast cancer; however, the HER2 protein is not consistently analyzed on formalin fixed tissues due to variability in fixation methods/times and the impact of this fixation on HER2 protein antigenicity. Formaldehyde 164-172 erb-b2 receptor tyrosine kinase 2 Homo sapiens 119-123 20740285-8 2010 For example, HER2 gene amplification is directly linked to the protein expression level in breast cancer; however, the HER2 protein is not consistently analyzed on formalin fixed tissues due to variability in fixation methods/times and the impact of this fixation on HER2 protein antigenicity. Formaldehyde 164-172 erb-b2 receptor tyrosine kinase 2 Homo sapiens 119-123 20931533-3 2010 HER2 expression level and HER2 gene copy number in formalin-fixed and paraffin embedded tumor samples were detected by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and the relationship between HER2 gene GH and clinicopathological characteristics were analyzed. Formaldehyde 51-59 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 20931533-3 2010 HER2 expression level and HER2 gene copy number in formalin-fixed and paraffin embedded tumor samples were detected by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and the relationship between HER2 gene GH and clinicopathological characteristics were analyzed. Formaldehyde 51-59 erb-b2 receptor tyrosine kinase 2 Homo sapiens 26-30 20931533-3 2010 HER2 expression level and HER2 gene copy number in formalin-fixed and paraffin embedded tumor samples were detected by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), and the relationship between HER2 gene GH and clinicopathological characteristics were analyzed. Formaldehyde 51-59 erb-b2 receptor tyrosine kinase 2 Homo sapiens 26-30 21151530-1 2010 We report here the results of the analytical validation of assays that measure HER2 total protein (H2T) and HER2 homodimer (H2D) expression in Formalin Fixed Paraffin Embedded (FFPE) breast cancer tumors as well as cell line controls. Formaldehyde 143-151 erb-b2 receptor tyrosine kinase 2 Homo sapiens 79-83 21151530-1 2010 We report here the results of the analytical validation of assays that measure HER2 total protein (H2T) and HER2 homodimer (H2D) expression in Formalin Fixed Paraffin Embedded (FFPE) breast cancer tumors as well as cell line controls. Formaldehyde 143-151 erb-b2 receptor tyrosine kinase 2 Homo sapiens 108-112 20169940-0 2010 [Comparison of two HER-2 FISH kits on formalin-fixed paraffin-embedded tissues: signal detection and simple procedure]. Formaldehyde 38-46 erb-b2 receptor tyrosine kinase 2 Homo sapiens 19-24 19734848-1 2009 Delay to formalin fixation may invalidate hormone receptors and HER2 analyses. Formaldehyde 9-17 erb-b2 receptor tyrosine kinase 2 Homo sapiens 64-68 19551847-2 2009 The evaluation of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) expression by immunohistochemistry (IHC) is performed routinely on formalin-fixed, paraffin-embedded needle-core (NC) or excision tissue block (TB) preparations, according to the American Society of Clinical Oncology/College of American Pathologist guidelines. Formaldehyde 189-197 erb-b2 receptor tyrosine kinase 2 Homo sapiens 116-120 19077907-7 2009 A national study of 109 participating clinical laboratories demonstrated a good correlation between a laboratory"s ability to properly stain formalin-fixed peptide controls to their ability in properly staining a 3+ HER-2 formalin-fixed tissue section mounted on the same slide (r=0.87). Formaldehyde 222-230 erb-b2 receptor tyrosine kinase 2 Homo sapiens 216-221 20076883-2 2009 METHODS: The immunohistochemical expression of Her-2/Neu was studied in 37 formalin-fixed paraffin-embedded tissue sections. Formaldehyde 75-83 erb-b2 receptor tyrosine kinase 2 Homo sapiens 47-56 19214113-0 2009 A novel proximity assay for the detection of proteins and protein complexes: quantitation of HER1 and HER2 total protein expression and homodimerization in formalin-fixed, paraffin-embedded cell lines and breast cancer tissue. Formaldehyde 156-164 erb-b2 receptor tyrosine kinase 2 Homo sapiens 102-106 19225135-4 2009 We developed a single-tube multiplex TaqMan (mERPR+HER2) assay to quantitate mRNA levels of ER, PR, HER2, and two housekeeping genes for breast cancer formalin-fixed, paraffin-embedded sections. Formaldehyde 151-159 erb-b2 receptor tyrosine kinase 2 Homo sapiens 51-55 19697624-3 2009 METHODS: The expression of ERBB2 has been analyzed immunohistochemically in formalin-fixed paraffin-embedded primary uterine sarcomas (n = 11). Formaldehyde 76-84 erb-b2 receptor tyrosine kinase 2 Homo sapiens 27-32 19087271-5 2008 C-erbB2 gene expression was analyzed in 30 different human tissue samples of primary invasive breast cancer, following formalin fixation and subsequent paraffin-embedding. Formaldehyde 119-127 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-7 18971621-1 2008 BACKGROUND: FISH and immunohistochemistry (IHC) on formalin-fixed paraffin embedded (FFPE) tissue are currently used in the clinical laboratory to determine HER2 status in invasive breast cancer patients. Formaldehyde 51-59 erb-b2 receptor tyrosine kinase 2 Homo sapiens 157-161 18483367-5 2008 Using immunohistochemical techniques, we analyzed the expressions of IGF-IR, EGFR, and HER2 on formalin-fixed paraffin-embedded specimens of surgically removed primary tumors. Formaldehyde 95-103 erb-b2 receptor tyrosine kinase 2 Homo sapiens 87-91 17451461-3 2008 The aim of the present study was to evaluate HER-2 expression by immunohistochemistry (IHC) in 12 USPC formalin-fixed, paraffin-embedded (FFPE) samples. Formaldehyde 103-111 erb-b2 receptor tyrosine kinase 2 Homo sapiens 45-50 17133373-9 2007 Using western blots and reverse phase protein microarrays, the receptor tyrosine kinase HER2, an important protein target for antibody based cancer treatment, was reliably measured in formalin-fixed breast cancer biopsy samples when compared with measurement by immunohistochemistry and fluorescence in situ hybridization; remarkably, immunohistochemically equivocal cases (score 2+) can be categorized according to HER2 protein abundance. Formaldehyde 184-192 erb-b2 receptor tyrosine kinase 2 Homo sapiens 88-92 20223002-4 2006 Fluorescence in situ hybridization for HER2 was performed on "touch" slides from frozen tumour samples or formalin-fixed, paraffin-embedded tissue. Formaldehyde 106-114 erb-b2 receptor tyrosine kinase 2 Homo sapiens 39-43 16949920-5 2006 In a combined analysis of immunohistochemistry and fluorescence in situ hybridization on 552 formalin-fixed and paraffin-embedded gastric cancer tissues, 38 cases were found to have HER2 amplification. Formaldehyde 93-101 erb-b2 receptor tyrosine kinase 2 Homo sapiens 182-186 16841374-5 2006 The approach has been validated by comparison of results with standard analyses for HER-2 amplification status in formalin-fixed, paraffin-embedded breast cancers and is applicable to the analysis of other clinically relevant genomic aberrations as well as of use in research investigations. Formaldehyde 114-122 erb-b2 receptor tyrosine kinase 2 Homo sapiens 84-89 16469859-9 2006 Multiplex aQRT-PCR reliably identified the HER-2 gene in microdissected breast cancer samples; in formalin-fixed, paraffin-embedded specimens; and in plasma circulating DNA from cancer patients. Formaldehyde 98-106 erb-b2 receptor tyrosine kinase 2 Homo sapiens 43-48 16761510-4 2006 Upon standard procedure of formalin fixation, embedding in paraffin and examination of slides stained with hematoxillin-eosin, a semiquantitaive method of evaluation of immunohistochemical expression of the HER-2 protein has been performed in all tissue samples of the tumor. Formaldehyde 27-35 erb-b2 receptor tyrosine kinase 2 Homo sapiens 207-212 15987433-3 2005 METHODS: In this study c-erbB2 and topoisomerase IIalpha protein were evaluated by immunohistochemistry in formalin-fixed paraffin-embedded tissue from 225 samples of primary breast cancer, obtained between 1986 and 1998. Formaldehyde 107-115 erb-b2 receptor tyrosine kinase 2 Homo sapiens 23-30 15606364-2 2004 Chromogenic in situ hybridization (CISH), in which the DNA probe is detected with an immunohistochemistry (IHC)-like peroxidase reaction, has been recently developed for the assessment of HER-2/neu status in formalin-fixed breast cancer specimens. Formaldehyde 208-216 erb-b2 receptor tyrosine kinase 2 Homo sapiens 188-197 15284539-5 2004 METHODS: MUC4 and ErbB2 receptor expression was localized by immunohistochemical studies using archival formalin-fixed and paraffin-embedded tissue. Formaldehyde 104-112 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-23 15019688-4 2004 Formalin-fixed paraffin-embedded pre-treatment tissue biopsies were examined immunohistochemically for the over-expression of the HER-2 protein and gene using the HercepTest and FISH assay. Formaldehyde 0-8 erb-b2 receptor tyrosine kinase 2 Homo sapiens 130-135 15054739-4 2004 METHODS: MUC4 antigens and ErbB2 receptor expression are localized by immunohistochemical studies that use archival formalin-fixed and paraffin-embedded tissue. Formaldehyde 116-124 erb-b2 receptor tyrosine kinase 2 Homo sapiens 27-32 15318940-2 2004 METHODS: We assessed Her-2/neu alteration using CISH on formalin-fixed paraffin-embedded primary invasive ductal carcinoma tumors in which IHC (CB11 antibody) had previously been performed, and we compared the results with IHC. Formaldehyde 56-64 erb-b2 receptor tyrosine kinase 2 Homo sapiens 21-26 15318940-2 2004 METHODS: We assessed Her-2/neu alteration using CISH on formalin-fixed paraffin-embedded primary invasive ductal carcinoma tumors in which IHC (CB11 antibody) had previously been performed, and we compared the results with IHC. Formaldehyde 56-64 erb-b2 receptor tyrosine kinase 2 Homo sapiens 27-30 12894498-4 2003 In our series of 6 patients with MTC, including 5 patients with adjacent C-cell hyperplasia, Her2/neu levels were immunohistochemically evaluated on formalin-fixed, paraffin-embedded tissues using c-erbB-2/Her-2/neu Oncoprotein Ab-17 monoclonal antibody(mAb). Formaldehyde 149-157 erb-b2 receptor tyrosine kinase 2 Homo sapiens 93-101 12894514-3 2003 Her2/neu levels were immunohistochemically detected on formalin-fixed, paraffin-embedded tissues using c-erbB-2/Her2/neu Oncoprotein Ab-17 monoclonal antibody(mAb). Formaldehyde 55-63 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 12894514-3 2003 Her2/neu levels were immunohistochemically detected on formalin-fixed, paraffin-embedded tissues using c-erbB-2/Her2/neu Oncoprotein Ab-17 monoclonal antibody(mAb). Formaldehyde 55-63 erb-b2 receptor tyrosine kinase 2 Homo sapiens 5-8 12894514-3 2003 Her2/neu levels were immunohistochemically detected on formalin-fixed, paraffin-embedded tissues using c-erbB-2/Her2/neu Oncoprotein Ab-17 monoclonal antibody(mAb). Formaldehyde 55-63 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-8 12715168-5 2003 Her2/neu was immunohistochemically detected on sections from formalin-fixed, paraffin-embedded tissues using c-erbB-2/Her-2/neu oncoprotein Ab-17 monoclonal antibody (mAb). Formaldehyde 61-69 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-8 12637466-3 2003 HER2 gene amplification (HER2_AMP) was evaluated by fluorescence in situ hybridization (FISH; Ventana Medical Systems HER-2/neu probe; Tucson, AZ), and HER2 protein overexpression (HER2_EXP) was evaluated by immunohistochemistry (IHC; Oncogene Science antibody Ab-3; Cambridge, MA) on parallel-cut formalin-fixed paraffin-embedded tissue sections. Formaldehyde 298-306 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 12820338-0 2003 Her2/neu analysis in formalin-fixed, paraffin-embedded breast carcinomas: comparison of immunohistochemistry and multiparameter DNA flow cytometry. Formaldehyde 21-29 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 12127055-3 2002 The analysis of HER-2 is usually performed on formalin-fixed paraffin tissue sections obtained from surgery. Formaldehyde 46-54 erb-b2 receptor tyrosine kinase 2 Homo sapiens 16-21 12474525-5 2002 differentially evaluate the effect of BCG immunotherapy in treated and untreated cohorts on the immunohistochemical expression of C-erbB-2 and VEGF in formalin-fixed paraffin-embedded sections of superficial and superficially invasive (Stage Ta-T1) transitional cell carcinoma of the bladder. Formaldehyde 151-159 erb-b2 receptor tyrosine kinase 2 Homo sapiens 130-138 11789735-0 2002 A formalin-fixed, paraffin-processed cell line standard for quality control of immunohistochemical assay of HER-2/neu expression in breast cancer. Formaldehyde 2-10 erb-b2 receptor tyrosine kinase 2 Homo sapiens 108-117 12658752-2 2002 The expression of c-erbB2 was detected immunohistochemically by monoclonal antibody against the gene on the formalin-fixed paraffin sections of 21 hydatidiform moles, 21 invasive moles, 20 choriocarcinomas and 30 normal placentas. Formaldehyde 108-116 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-25 11688845-7 2001 This article reviews data on the HER-2 gene and protein, discusses their association with clinical outcomes, and proposes a strategy for screening for HER-2 excess in formalin-fixed specimens of breast carcinoma. Formaldehyde 167-175 erb-b2 receptor tyrosine kinase 2 Homo sapiens 151-156 11820720-2 2001 Immunohistochemical study of the expression of c-erbB-2 was performed in formalin-fixed, paraffin-embedded sections from 82 gastric adenocarcinomas using polyclonal antibody. Formaldehyde 73-81 erb-b2 receptor tyrosine kinase 2 Homo sapiens 47-55 11326673-4 2000 MATERIALS AND METHODS: In primary breast carcinoma samples from 115 women undergoing surgery in our department in 1999, a polyclonal rabbit antibody to human c-erbB-2 oncoprotein was used for immunohistochemical assessment of the c-erbB-2 expression in formalin-fixed paraffin-embedded material. Formaldehyde 253-261 erb-b2 receptor tyrosine kinase 2 Homo sapiens 158-166 10824754-5 2000 Formalin-fixed, paraffin-embedded tissue was processed for ploidy, S phase fraction, and immunohistochemical detection of estrogen and progesterone receptors plus expression of p53 and erbB-2 protein. Formaldehyde 0-8 erb-b2 receptor tyrosine kinase 2 Homo sapiens 185-191 10502729-4 1999 In a retrospective study of 62 formalin-fixed paraffin-embedded invasive EOBC (diagnosed at 35 years or less), the amplification status of the c-erbB-2 gene detected by fluorescence in situ hybridization (FISH) using a unique sequence probe was compared with p53 protein accumulation measured by immunohistochemistry (IHC) and phenotypic features. Formaldehyde 31-39 erb-b2 receptor tyrosine kinase 2 Homo sapiens 143-151 10560479-9 1999 All formalin-fixed tissue specimens that were determined to positively express c-erbB-2 were also found to be positive on the alcohol-fixed smears. Formaldehyde 4-12 erb-b2 receptor tyrosine kinase 2 Homo sapiens 79-87 10560479-10 1999 CONCLUSION: The incidence of c-erbB-2 expression in fresh cytologic material is significantly higher (P < .05) than in formalin-fixed, paraffin-embedded tissue. Formaldehyde 122-130 erb-b2 receptor tyrosine kinase 2 Homo sapiens 29-37 9570628-3 1998 METHODS: A monoclonal antibody against the c-erbB-2 oncoprotein has been used immunocytochemically in a retrospective study of formalin-fixed, paraffin-embedded samples from 28 consecutive intestinal-type adenocarcinomas (ITACs) of the nose and paranasal sinuses. Formaldehyde 127-135 erb-b2 receptor tyrosine kinase 2 Homo sapiens 43-51 9520947-0 1998 Amplification of c-erbB-2 in gastric cancer: detection in formalin-fixed, paraffin-embedded tissue by fluorescence in situ hybridization. Formaldehyde 58-66 erb-b2 receptor tyrosine kinase 2 Homo sapiens 17-25 9267826-10 1997 HER-2/neu oncogene amplification can be assessed using automated FISH on formalin-fixed, paraffin-embedded tissue. Formaldehyde 73-81 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-5 9267826-10 1997 HER-2/neu oncogene amplification can be assessed using automated FISH on formalin-fixed, paraffin-embedded tissue. Formaldehyde 73-81 erb-b2 receptor tyrosine kinase 2 Homo sapiens 6-9 8918900-1 1996 The relationship between expression of the c-erbB-2 proto-oncogene and the biology of breast cancer has been investigated widely, most studies using immunohistochemistry in formalin-fixed, paraffin-embedded tissues. Formaldehyde 173-181 erb-b2 receptor tyrosine kinase 2 Homo sapiens 43-51 8997010-1 1996 Using a polyclonal antibody against the c-erbB-2 gene product, an immunohistochemical study on the expression of c-erbB-2 oncoprotein was performed in routine formalin-fixed, paraffin-embedded tissue sections from 119 patients with primary breast cancer. Formaldehyde 159-167 erb-b2 receptor tyrosine kinase 2 Homo sapiens 113-121 9387524-2 1996 METHODS: The amplification and expression of c-erbB2 oncogene were determined from formalin-fixed, paraffin-embedded tissues of 25 normal, 31 hyperplastic, and 72 malignant samples of the endometrium in 128 patients, using differential polymerase chain reaction (DPCR) and an immunohistochemical technique. Formaldehyde 83-91 erb-b2 receptor tyrosine kinase 2 Homo sapiens 45-52 7503357-2 1995 We describe here a novel microdissection technique employed to correlate HER2/neu (HER2) immunohistochemical staining with HER2 genetic analysis in formalin-fixed, paraffin-embedded breast tissue. Formaldehyde 148-156 erb-b2 receptor tyrosine kinase 2 Homo sapiens 73-81 7503357-2 1995 We describe here a novel microdissection technique employed to correlate HER2/neu (HER2) immunohistochemical staining with HER2 genetic analysis in formalin-fixed, paraffin-embedded breast tissue. Formaldehyde 148-156 erb-b2 receptor tyrosine kinase 2 Homo sapiens 73-77 7503357-2 1995 We describe here a novel microdissection technique employed to correlate HER2/neu (HER2) immunohistochemical staining with HER2 genetic analysis in formalin-fixed, paraffin-embedded breast tissue. Formaldehyde 148-156 erb-b2 receptor tyrosine kinase 2 Homo sapiens 83-87 8595375-1 1995 A quantitative method of polymerase chain reaction (PCR) using both digoxigenin and radioactive labelled probes has been used for the detection of the c-erbB-2 proto-oncogene amplification in breast carcinomas with formalin-fixed paraffin-embedded tissue sections. Formaldehyde 215-223 erb-b2 receptor tyrosine kinase 2 Homo sapiens 151-159 7591299-4 1995 This fluorescent differential polymerase chain reaction (fd-PCR) method was used for quantificative determination of ERBB2 gene amplification in 195 formalin-fixed, paraffin-embedded breast carcinoma tissues. Formaldehyde 149-157 erb-b2 receptor tyrosine kinase 2 Homo sapiens 117-122 7547494-1 1995 Expression of c-erbB-2 and p53 protein was analysed retrospectively by immunohistochemistry in formalin-fixed tissue samples from 293 patients with colorectal adenocarcinoma. Formaldehyde 95-103 erb-b2 receptor tyrosine kinase 2 Homo sapiens 14-22 7882282-5 1995 METHODS: Immunohistochemical staining for HER was performed on 10% formalin fixed paraffin embedded primary carcinomas from 440 patients with negative axillary lymph nodes with a median follow-up of 119 months. Formaldehyde 67-75 erb-b2 receptor tyrosine kinase 2 Homo sapiens 42-45 7774494-2 1995 To this effect, we compared the expression of neu oncoprotein in formalin-fixed paraffin-embedded breast cancers and the corresponding fine-needle aspirates of these cancers. Formaldehyde 65-73 erb-b2 receptor tyrosine kinase 2 Homo sapiens 46-49 21597698-1 1995 Overexpression of PCNA (more than 25% positive tumour cells) and positivity of c-erbB-2 oncoprotein were immunohistochemically demonstrated in 490 formalin-fixed and paraffin-embedded breast carcinomas. Formaldehyde 147-155 erb-b2 receptor tyrosine kinase 2 Homo sapiens 79-87 7911272-3 1994 We used immunocytochemical techniques to retrospectively study the expression of the c-erb B-2 oncogene product in formalin-fixed, paraffin-embedded trophoblastic tissues as a potential indicator of the development of persistent gestational trophoblastic disease. Formaldehyde 115-123 erb-b2 receptor tyrosine kinase 2 Homo sapiens 85-94 7909490-4 1994 METHODS: The immunostaining for HER-2/neu oncoprotein was performed on formaldehyde-solution-fixed, paraffin-embedded sections of 888 node-positive breast cancers resected between 1980 and 1986 and for which a follow-up of 2.5-10.5 years was available. Formaldehyde 71-83 erb-b2 receptor tyrosine kinase 2 Homo sapiens 32-41 11578406-1 1994 The expression of c-erbB-2 oncogene has been studied in 74 formalin fixed paraffin embedded invasive epithelial ovarian malignancies using a monoclonal c-erbB-2 protein antibody. Formaldehyde 59-67 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-26 7907940-4 1994 Overexpression of c-erbB-2 oncoprotein was studied by immunohistochemical alkaline phosphatase-antialkaline phosphatase staining of formalin fixed, paraffin embedded tissue sections of the primary tumor. Formaldehyde 132-140 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-26 7909307-0 1994 c-erbB-2 amplification in breast cancer: detection in formalin-fixed, paraffin-embedded tissue by in situ hybridization. Formaldehyde 54-62 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-8 7909307-1 1994 We describe a sensitive and practical in situ hybridization method, using a digoxigenin-labeled probe, for the detection of c-erbB-2 amplification in breast cancer in formalin-fixed, paraffin-embedded tissue sections. Formaldehyde 167-175 erb-b2 receptor tyrosine kinase 2 Homo sapiens 124-132 7905364-1 1994 BACKGROUND: A polyclonal antihuman c-erbB-2 oncoprotein antibody recognized c-erbB-2 oncoprotein in routinely formaldehyde-fixed, paraffin-embedded specimens. Formaldehyde 110-122 erb-b2 receptor tyrosine kinase 2 Homo sapiens 35-43 7905364-1 1994 BACKGROUND: A polyclonal antihuman c-erbB-2 oncoprotein antibody recognized c-erbB-2 oncoprotein in routinely formaldehyde-fixed, paraffin-embedded specimens. Formaldehyde 110-122 erb-b2 receptor tyrosine kinase 2 Homo sapiens 76-84 7902202-4 1993 METHODS: Immunohistochemical reactivity to c-erbB-2 protein was examined in formalin-fixed, paraffin-embedded tissue sections from patients with gastric cancer. Formaldehyde 76-84 erb-b2 receptor tyrosine kinase 2 Homo sapiens 43-51 7901825-1 1993 c-erbB-2 gene amplification was examined by slot blot hybridization using DNAs extracted from formalin-fixed and paraffin-embedded tissues of primary gastric carcinomas. Formaldehyde 94-102 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-8 8104689-8 1993 Importantly, a series of molecularly characterized breast cancer specimens were used to confirm that the antibody used was of sufficient sensitivity and specificity to identify those cancers overexpressing the HER-2/neu protein in this formalin-fixed, paraffin-embedded tissue cohort. Formaldehyde 236-244 erb-b2 receptor tyrosine kinase 2 Homo sapiens 210-219 8397058-3 1993 RESULTS: C-erbB-2 protein was located on the cell membrane of cancer cells in formalin-fixed, paraffin-embedded sections. Formaldehyde 78-86 erb-b2 receptor tyrosine kinase 2 Homo sapiens 9-17 8101229-5 1993 Using an immunoperoxidase technique, we stained archival formalin-fixed, paraffin-embedded tissue sections with a monoclonal antibody to the 185-kilodalton protein product (p185) of the HER-2/neu gene. Formaldehyde 57-65 erb-b2 receptor tyrosine kinase 2 Homo sapiens 186-195 8099533-6 1993 METHODS: The expression of c-erbB-2 protein was immunohistochemically investigated in the formaldehyde-fixed, paraffin-embedded tissues of 17 cases of IMHN, and 14 cases of pancreatic ductal cell carcinoma (8 cases with lymph node metastasis), using polyclonal and monoclonal c-erbB-2(p185) antibodies by the avidin-biotin method. Formaldehyde 90-102 erb-b2 receptor tyrosine kinase 2 Homo sapiens 27-35 7687354-1 1993 Although over-expression of the C-erbB-2 oncogene product can be detected on formalin-fixed, paraffin-embedded sections from tumours of the human breast, fixation results in a considerable loss of immunostaining for this protein. Formaldehyde 77-85 erb-b2 receptor tyrosine kinase 2 Homo sapiens 32-40 8104720-1 1993 A monoclonal antibody, NCL-CB11, raised against a synthetic peptide from the predicted sequence of the c-erbB-2 protein has been used immunohistochemically in a retrospective study of formalin-fixed paraffin embedded breast neoplasm biopsies. Formaldehyde 184-192 erb-b2 receptor tyrosine kinase 2 Homo sapiens 103-111 8094977-2 1993 The expression of c-erbB-2 oncoprotein was determined on formalin-fixed paraffin-embedded tissue, using a monoclonal anti-c-erbB-2 antibody by the avidin-biotin immunoperoxidase method. Formaldehyde 57-65 erb-b2 receptor tyrosine kinase 2 Homo sapiens 18-26 8093216-1 1993 An immunohistochemical study of the c-erbB-2 protein was conducted on formalin-fixed paraffin-embedded tissue sections from 136 primary gastric carcinomas and 50 metastatic lymph-node tumors obtained at gastrectomy. Formaldehyde 70-78 erb-b2 receptor tyrosine kinase 2 Homo sapiens 36-44 8094884-1 1993 We studied the overexpression of c-erbB-2 protein in formalin-fixed, paraffin-embedded tissues from matched primary tumors and metastatic lymph nodes derived from each of 95 breast carcinoma patients. Formaldehyde 53-61 erb-b2 receptor tyrosine kinase 2 Homo sapiens 33-41 7505056-3 1993 Using a monoclonal antibody, AB-3, which recognizes -COOH-terminal of neu oncoprotein, we have analyzed immunohistochemically the expression of this protein in buffered formalin and Zamboni fluid-fixed surgically removed tissues. Formaldehyde 169-177 erb-b2 receptor tyrosine kinase 2 Homo sapiens 70-73 7509541-1 1993 Overexpression of p53 and erbB-2 was studied by immunohistochemistry in formalin-fixed tissue samples of 179 patients with transitional cell carcinoma of the urinary bladder. Formaldehyde 72-80 erb-b2 receptor tyrosine kinase 2 Homo sapiens 26-32 1358427-4 1992 METHODS: Immunohistologic study of the c-erbB-2 gene product was done in formaldehyde-fixed paraffin-embedded tissue specimens obtained from 88 transitional cell carcinomas of the human urinary bladder. Formaldehyde 73-85 erb-b2 receptor tyrosine kinase 2 Homo sapiens 39-47 1358045-3 1992 The present study evaluates c-erbB-2 protein over-production in sarcomas and in small round-cell tumors of childhood by applying an immunohistochemical technique to formalin-fixed, paraffin-embedded tissues. Formaldehyde 165-173 erb-b2 receptor tyrosine kinase 2 Homo sapiens 28-36 1356909-1 1992 Commercially available monoclonal antibodies were tested for their ability to detect increased levels of c-erbB-2 protein in formalin-fixed, paraffin-embedded breast carcinomas. Formaldehyde 125-133 erb-b2 receptor tyrosine kinase 2 Homo sapiens 105-113 1350695-1 1992 The expression of the c-erbB-2 proto-oncogene product was investigated immunohistochemically in 474 formalin-fixed and paraffin-embedded human breast tissue samples. Formaldehyde 100-108 erb-b2 receptor tyrosine kinase 2 Homo sapiens 22-30 1687240-1 1991 Detection of c-erbB-2 oncogene product expression by monoclonal antibody staining (avidin-biotin technique) in formalin-fixed, paraffin-embedded atypical hyperplasias (AH, n = 20), intraductal carcinomas (IDCA, n = 27) and invasive carcinomas (INVCA, n = 48) was compared to ploidy determinations obtained by flow cytometry (INVCA) or image analysis (AH, IDCA). Formaldehyde 111-119 erb-b2 receptor tyrosine kinase 2 Homo sapiens 13-21 1942310-12 1991 Immunohistochemical analysis with a p185HER-2/neu polyclonal antibody, on formalin fixed, paraffin embedded tissue, was specific for HER-2/neu overexpression but not as sensitive as Northern analysis. Formaldehyde 74-82 erb-b2 receptor tyrosine kinase 2 Homo sapiens 46-49 1942310-12 1991 Immunohistochemical analysis with a p185HER-2/neu polyclonal antibody, on formalin fixed, paraffin embedded tissue, was specific for HER-2/neu overexpression but not as sensitive as Northern analysis. Formaldehyde 74-82 erb-b2 receptor tyrosine kinase 2 Homo sapiens 40-49 1676930-3 1991 The authors retrospectively evaluated 62 cases of lobular neoplasia for expression of the c-erbB-2 gene product on formalin-fixed, deparaffinized sections, using two monoclonal anti-erbB-2 (p185) antibodies (c-neu Ab3 and m-erb) and one polyclonal anti-erbB-2 antibody (pAb 1) by the avidin-biotin-peroxidase method. Formaldehyde 115-123 erb-b2 receptor tyrosine kinase 2 Homo sapiens 90-98 1679345-9 1991 It was concluded that both fresh-frozen and paraffin-embedded, formalin-fixed material is suitable for assessment of HER-2/neu protein overexpression by image analysis and provides comparable HER-2/neu expression values in most cases. Formaldehyde 63-71 erb-b2 receptor tyrosine kinase 2 Homo sapiens 117-126 1679345-9 1991 It was concluded that both fresh-frozen and paraffin-embedded, formalin-fixed material is suitable for assessment of HER-2/neu protein overexpression by image analysis and provides comparable HER-2/neu expression values in most cases. Formaldehyde 63-71 erb-b2 receptor tyrosine kinase 2 Homo sapiens 192-201 1670998-1 1991 Using a polyclonal antibody that is monospecific for the erbB-2 oncogene product, an immunohistochemical study of the expression of erbB-2 protein was performed in formalin-fixed paraffin-embedded tissue sections from 260 primary gastric cancers. Formaldehyde 164-172 erb-b2 receptor tyrosine kinase 2 Homo sapiens 132-138 1977159-2 1990 We have performed immunohistochemical staining using a polyclonal antibody to the Neu oncoprotein on formalin-fixed material from normal breast, benign breast lesions, and 102 stage I node-negative breast cancers. Formaldehyde 101-109 erb-b2 receptor tyrosine kinase 2 Homo sapiens 82-85 1977159-8 1990 This study shows that the Neu oncoprotein can be demonstrated on formalin-fixed material from normal, benign, and malignant breast lesions. Formaldehyde 65-73 erb-b2 receptor tyrosine kinase 2 Homo sapiens 26-29 1973458-0 1990 NCL-CB11, a new monoclonal antibody recognizing the internal domain of the c-erbB-2 oncogene protein effective for use on formalin-fixed, paraffin-embedded tissue. Formaldehyde 122-130 erb-b2 receptor tyrosine kinase 2 Homo sapiens 75-83 1698166-4 1990 The monoclonal antibodies to c-erbB-2 and EGF-R immunostained subpopulations of tumour cells on sections of formalin-fixed, paraffin wax embedded human infiltrating and invasive ductal carcinomas of breast. Formaldehyde 108-116 erb-b2 receptor tyrosine kinase 2 Homo sapiens 29-37 34289525-1 2021 We previously reported the usefulness of droplet digital polymerase chain reaction (ddPCR) for the assessment of Human epithelial growth factor receptor 2 (HER2) gene amplification in breast cancer using formalin-fixed and paraffin-embedded sections. Formaldehyde 204-212 erb-b2 receptor tyrosine kinase 2 Homo sapiens 156-160 34136904-0 2021 Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Formaldehyde 121-129 erb-b2 receptor tyrosine kinase 2 Homo sapiens 24-58 34136904-3 2021 We evaluated the analytical performance of a targeted mass spectrometry-based assay for quantifying HER2 protein in formalin-fixed paraffin-embedded (FFPE) and frozen BC biopsies. Formaldehyde 116-124 erb-b2 receptor tyrosine kinase 2 Homo sapiens 100-104 35574415-3 2022 Methods: HER2 protein expression was tested by immunohistochemistry (IHC) in formalin-fixed, paraffin-embedded (FFPE) samples from 4,836 CRC patients in our institution. Formaldehyde 77-85 erb-b2 receptor tyrosine kinase 2 Homo sapiens 9-13 35565309-4 2022 Droplet digital PCR (ddPCR) was used to implement the assessment of HER2 status in formalin-fixed paraffin-embedded (FFPE) tumor DNA from a retrospective cohort (86 patients) and in cell-free DNA (cfDNA) samples from a prospective cohort (28 patients). Formaldehyde 83-91 erb-b2 receptor tyrosine kinase 2 Homo sapiens 68-72