PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31593829-3 2019 Based on the idea that the optimization of the components is required to improve transfection efficiency, the present study aimed to formulate and characterize cholesteryl oleate-containing solid-lipid nanoparticles (CO-SLNs) incorporating protamine (P) to condense DNA to produce P:DNA:CO-SLN complexes as non-viral vectors for gene delivery with reduced cytotoxicity and high cellular uptake efficiency. Insulin, Isophane 240-249 sarcolipin Homo sapiens 220-223 31593829-8 2019 Transfection studies in human embryonic kidney (HEK293T) cells showed the versatility of adding protamine to efficiently transfect cells, widening the potential applications of CO-SLN-based vectors, since the incorporation of protamine induced almost a 200-fold increase in the transfection capacity of CO-SLNs without toxicity. Insulin, Isophane 96-105 sarcolipin Homo sapiens 180-183