PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15126635-0 2004 Nedd4.1-mediated ubiquitination and subsequent recruitment of Tsg101 ensure HTLV-1 Gag trafficking towards the multivesicular body pathway prior to virus budding. Glycosaminoglycans 83-86 NEDD4 E3 ubiquitin protein ligase Homo sapiens 0-5 15126635-2 2004 The Gag proteins of oncoretroviruses possess a PPxY motif that recruits a ubiquitin ligase from the Nedd4 family, whereas those of the human immunodeficiency virus interact through a PTAP motif with Tsg101, a protein of the ESCRT-1 complex. Glycosaminoglycans 4-7 NEDD4 E3 ubiquitin protein ligase Homo sapiens 100-105 15126635-5 2004 We show that Nedd4.1, but not Nedd4.2, is recruited by the PPPY motif of Gag and subsequently catalyzes Gag ubiquitination. Glycosaminoglycans 73-76 NEDD4 E3 ubiquitin protein ligase Homo sapiens 13-20 15126635-5 2004 We show that Nedd4.1, but not Nedd4.2, is recruited by the PPPY motif of Gag and subsequently catalyzes Gag ubiquitination. Glycosaminoglycans 104-107 NEDD4 E3 ubiquitin protein ligase Homo sapiens 13-20 15126635-6 2004 We also demonstrate that Gag interacts first with Nedd4.1 at the plasma membrane and then with Tsg101 in late endosomes/MVBs. Glycosaminoglycans 25-28 NEDD4 E3 ubiquitin protein ligase Homo sapiens 50-57 15126635-8 2004 Our findings indicate that Nedd4.1 and Tsg101 act successively in the assembly process of HTLV-1 to ensure proper Gag trafficking through the endocytic pathway up to late endosomes where the late steps of retroviral release occur. Glycosaminoglycans 114-117 NEDD4 E3 ubiquitin protein ligase Homo sapiens 27-32