PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16749903-10 2006 Gag and Env only co-localize at the trans-Golgi network, suggesting that Env-Gag interactions that are required for viral egress from the cell, occurs at this site. Glycosaminoglycans 0-3 endogenous retrovirus group K member 20 Homo sapiens 73-76 17173051-5 2007 Increasing breadth of Gag-specific responses was associated with decreasing viremia and increasing Env breadth with increasing viremia. Glycosaminoglycans 22-25 endogenous retrovirus group K member 20 Homo sapiens 99-102 20531016-3 2010 Gag-Env pseudovirions recapitulate the native trimer and could serve as an effective epitope presentation platform for study of the neutralizing antibody response in HIV-infected individuals. Glycosaminoglycans 0-3 endogenous retrovirus group K member 20 Homo sapiens 4-7 20531016-5 2010 By blue native gel shift assays, Gag-Env pseudovirions were shown to contain native trimers that were competent for binding to neutralizing monoclonal antibodies. Glycosaminoglycans 33-36 endogenous retrovirus group K member 20 Homo sapiens 37-40 20531016-12 2010 These data demonstrate that Gag-Env pseudovirions recapitulate CD4 and coreceptor binding pocket antigenic structures and can facilitate identification of B-cell clones that secrete neutralizing antibodies. Glycosaminoglycans 28-31 endogenous retrovirus group K member 20 Homo sapiens 32-35 19458002-5 2009 Further, we document that repression of Env-induced cell-cell fusion by tetraspanins depends on the presence of viral Gag, and we demonstrate that fusion repression requires the recruitment of Env by Gag to tetraspanin-enriched microdomains (TEMs). Glycosaminoglycans 118-121 endogenous retrovirus group K member 20 Homo sapiens 40-43 19458002-5 2009 Further, we document that repression of Env-induced cell-cell fusion by tetraspanins depends on the presence of viral Gag, and we demonstrate that fusion repression requires the recruitment of Env by Gag to tetraspanin-enriched microdomains (TEMs). Glycosaminoglycans 200-203 endogenous retrovirus group K member 20 Homo sapiens 40-43 19458002-5 2009 Further, we document that repression of Env-induced cell-cell fusion by tetraspanins depends on the presence of viral Gag, and we demonstrate that fusion repression requires the recruitment of Env by Gag to tetraspanin-enriched microdomains (TEMs). Glycosaminoglycans 200-203 endogenous retrovirus group K member 20 Homo sapiens 193-196 19158248-3 2009 Cell lines derived from peripheral blood mononuclear cells stimulated in vitro with peptides representing targeted Gag epitopes consistently neutralized HIV better than Env-specific lines from the same person, although ineffective inhibition of virus replication is not a universal characteristic of Env-specific responses at the clonal level. Glycosaminoglycans 115-118 endogenous retrovirus group K member 20 Homo sapiens 300-303 19020825-5 2009 The viral envelope (Env) glycoproteins associate with Gag during the assembly process. Glycosaminoglycans 54-57 endogenous retrovirus group K member 20 Homo sapiens 20-23 16749903-10 2006 Gag and Env only co-localize at the trans-Golgi network, suggesting that Env-Gag interactions that are required for viral egress from the cell, occurs at this site. Glycosaminoglycans 77-80 endogenous retrovirus group K member 20 Homo sapiens 8-11 16749903-10 2006 Gag and Env only co-localize at the trans-Golgi network, suggesting that Env-Gag interactions that are required for viral egress from the cell, occurs at this site. Glycosaminoglycans 77-80 endogenous retrovirus group K member 20 Homo sapiens 73-76 15242543-6 2004 Stimulations with five peptide pools for Gag and seven peptide pools for Env revealed epitopes for cellular immune responses throughout Gag and Env. Glycosaminoglycans 136-139 endogenous retrovirus group K member 20 Homo sapiens 73-76 16297422-6 2006 With one exception, all Bet- and/or Env-positive sera were also positive for Gag. Glycosaminoglycans 77-80 endogenous retrovirus group K member 20 Homo sapiens 36-39 15693627-9 2004 For patients with known infection dates, the estimated dates of the coalescent events obtained using molecular clock calculations based on a newly developed Bayesian method in gag + env were in agreement with the actual infection dates. Glycosaminoglycans 176-181 endogenous retrovirus group K member 20 Homo sapiens 182-185 15194752-8 2004 Assuming 1,200 to 2,500 Gag molecules per virion, this corresponds to 7 to 16 Env trimers per SIV239 virion particle. Glycosaminoglycans 24-27 endogenous retrovirus group K member 20 Homo sapiens 78-81 15644496-5 2005 Env also binds cell-surface glycosaminoglycans. Glycosaminoglycans 28-46 endogenous retrovirus group K member 20 Homo sapiens 0-3 15345313-3 2004 Continuous expression of Gag and Env proteins was detected in stably transduced BLCL, which induced Gag- or Env-specific T cell responses, as measured by both IFNgamma-ELISPOT and chromium release assays, upon in vitro stimulation of PBMC from the SHIV89.6P-infected monkeys. Glycosaminoglycans 25-28 endogenous retrovirus group K member 20 Homo sapiens 108-111 15345313-3 2004 Continuous expression of Gag and Env proteins was detected in stably transduced BLCL, which induced Gag- or Env-specific T cell responses, as measured by both IFNgamma-ELISPOT and chromium release assays, upon in vitro stimulation of PBMC from the SHIV89.6P-infected monkeys. Glycosaminoglycans 100-103 endogenous retrovirus group K member 20 Homo sapiens 33-36 11483744-0 2001 Specific interaction of a novel foamy virus Env leader protein with the N-terminal Gag domain. Glycosaminoglycans 83-86 endogenous retrovirus group K member 20 Homo sapiens 44-47 12852860-4 2003 In the presence of Env, Gag is rerouted from lysosomes to transferrin-positive endosomes, and virion production becomes highly sensitive to drugs poisoning vesicular and endosomal traffic. Glycosaminoglycans 24-27 endogenous retrovirus group K member 20 Homo sapiens 19-22 14668432-6 2003 Combined with biochemical analysis of gag/env ratios in virions, these trimer counts allow calculation of the number of gag molecules per virion, yielding an average value of approximately 1400. Glycosaminoglycans 120-123 endogenous retrovirus group K member 20 Homo sapiens 42-45 11779349-4 2001 When the ability of the Env mutants to associate with SIV Gag particles was examined, we found that deletion of 20 to 80 residues from the carboxyl terminus of the SIV TM cytoplasmic tail abrogated the incorporation of the Env glycoprotein into particles. Glycosaminoglycans 58-61 endogenous retrovirus group K member 20 Homo sapiens 24-27 11779349-5 2001 By contrast, further truncation of the SIV TM protein by 100 to 140 amino acids restored the ability of the Env protein to associate with Gag particles. Glycosaminoglycans 138-141 endogenous retrovirus group K member 20 Homo sapiens 108-111 11483744-9 2001 The purified N-terminal domain of FFV Gag specifically interacted with synthetic peptides and a defined protein domain derived from the N-terminal Env leader protein. Glycosaminoglycans 38-41 endogenous retrovirus group K member 20 Homo sapiens 147-150 11483744-11 2001 The interaction with Gag required residues within the novel virion-associated FFV Env leader protein of about 16.5 kDa. Glycosaminoglycans 21-24 endogenous retrovirus group K member 20 Homo sapiens 82-85 9934708-5 1999 The relative rates of change for FV structural proteins were Pol < Env < Gag in increasing order, which differs from all other retroviruses. Glycosaminoglycans 79-82 endogenous retrovirus group K member 20 Homo sapiens 70-73 11435565-5 2001 In addition, the strict requirement of Env expression for capsid budding can be bypassed by addition of a PM-targeting signal to Gag. Glycosaminoglycans 129-132 endogenous retrovirus group K member 20 Homo sapiens 39-42 11435565-7 2001 The necessity of Env expression for particle egress is most probably due to the lack of a membrane-targeting signal within FV Gag to direct capsids to the PM for release and indicates that Gag-Env interactions are essential to drive particle budding. Glycosaminoglycans 189-192 endogenous retrovirus group K member 20 Homo sapiens 17-20 11435565-7 2001 The necessity of Env expression for particle egress is most probably due to the lack of a membrane-targeting signal within FV Gag to direct capsids to the PM for release and indicates that Gag-Env interactions are essential to drive particle budding. Glycosaminoglycans 189-192 endogenous retrovirus group K member 20 Homo sapiens 193-196 10618286-3 2000 Denatured Env-Gag did not cause any effect on the NK cell activity of LGL. Glycosaminoglycans 14-17 endogenous retrovirus group K member 20 Homo sapiens 10-13 1850037-10 1991 Deletion of regions in gag, which was previously shown to contain a cis-acting negative regulator of splicing, resulted in a corresponding increase of both spliced viral mRNAs and a decrease in unspliced RNA, suggesting that this element suppressed both env and src splicing. Glycosaminoglycans 23-26 endogenous retrovirus group K member 20 Homo sapiens 254-257 8892955-9 1996 We conclude that recombination in the gag gene is highly frequent among the major env subtypes and that selection of recombinants is apparently based on particularly beneficial combinations of gag and env gene products. Glycosaminoglycans 38-41 endogenous retrovirus group K member 20 Homo sapiens 82-85 8661411-3 1996 When cells expressing Env proteins in the absence of Gag were examined by immunoelectron microscopy, clusters of Env protein and membrane vesicles containing Env proteins were observed at cell surfaces. Glycosaminoglycans 53-56 endogenous retrovirus group K member 20 Homo sapiens 22-25 8204207-4 1994 The env-gag-induced proliferative responses of lymphocytes from normal subjects were significantly suppressed when cultures contained only higher levels of ETOH (0.2% and 0.3%), whereas ETOH even at a lower level (0.1%) produced significant suppression of the env-gag-induced proliferation of lymphocytes only from AIDS patients. Glycosaminoglycans 8-11 endogenous retrovirus group K member 20 Homo sapiens 4-7 8204207-4 1994 The env-gag-induced proliferative responses of lymphocytes from normal subjects were significantly suppressed when cultures contained only higher levels of ETOH (0.2% and 0.3%), whereas ETOH even at a lower level (0.1%) produced significant suppression of the env-gag-induced proliferation of lymphocytes only from AIDS patients. Glycosaminoglycans 8-11 endogenous retrovirus group K member 20 Homo sapiens 260-263 8204207-4 1994 The env-gag-induced proliferative responses of lymphocytes from normal subjects were significantly suppressed when cultures contained only higher levels of ETOH (0.2% and 0.3%), whereas ETOH even at a lower level (0.1%) produced significant suppression of the env-gag-induced proliferation of lymphocytes only from AIDS patients. Glycosaminoglycans 264-267 endogenous retrovirus group K member 20 Homo sapiens 4-7 8107246-7 1994 This response was observed in two animals (one VV-Env and one Gag-Env). Glycosaminoglycans 62-65 endogenous retrovirus group K member 20 Homo sapiens 66-69 8107246-11 1994 Active infection developed in all controls and two of three VV-Gag-Env-immunized animals. Glycosaminoglycans 63-66 endogenous retrovirus group K member 20 Homo sapiens 67-70 8648695-2 1996 The 11.2-kb provirus displays a complex expression pattern capable of encoding accessory proteins and is unique in the predicted location of the env initiation codon and signal peptide upstream of gag and the common splice donor site. Glycosaminoglycans 197-200 endogenous retrovirus group K member 20 Homo sapiens 145-148 7618287-5 1995 In addition, the incorporation of the envelope glycoprotein (Env) into the Gag-made particles was investigated. Glycosaminoglycans 75-78 endogenous retrovirus group K member 20 Homo sapiens 61-64 8356792-3 1993 The internal deletions of the latter encompass most of the gag to env region. Glycosaminoglycans 59-62 endogenous retrovirus group K member 20 Homo sapiens 66-69 1727880-5 1992 Anti-gag p24 antibody was strongly correlated with antibodies to other env products, specifically gp41 and gp160. Glycosaminoglycans 5-8 endogenous retrovirus group K member 20 Homo sapiens 71-74 1658356-9 1991 This observation, particularly in reference to our earlier finding of extensive mutations in the gag gene, reveals a target area for potentially productive homologous recombination upstream of the functional endogenous env gene. Glycosaminoglycans 97-100 endogenous retrovirus group K member 20 Homo sapiens 219-222 2065891-3 1991 Fixed nucleic acid substitutions in the gag gene appear to be caused by random fixation of selectively neutral mutants, whereas nonrandom fixation of selectively advantageous mutants, as has been observed for MHC molecules and serine protease inhibitors, appears to be operational for some hypervariable env gene regions. Glycosaminoglycans 40-43 endogenous retrovirus group K member 20 Homo sapiens 304-307 35201897-11 2022 These genetic defects were concentrated in the env region compared to gag and pol, likely a reflection of viral immune escape in env during untreated HIV-1 infection. Glycosaminoglycans 70-73 endogenous retrovirus group K member 20 Homo sapiens 129-132 2012254-8 1991 Reactivity to three or more gag-encoded proteins was found in 85% (45/53) of ELISA-positive, Western blot-indeterminate sera, and 30% (16/53) reacted to p19 and an env gene product but lacked reactivity to p24. Glycosaminoglycans 28-31 endogenous retrovirus group K member 20 Homo sapiens 164-167 3304356-6 1987 The quantitative analysis of sera with env and gag antigens by ELISA showed AIDS patients had very low gag reactivity while retaining high env reactivity. Glycosaminoglycans 103-106 endogenous retrovirus group K member 20 Homo sapiens 39-42 3304356-8 1987 This correlation and the analysis of sera with both the ENV and GAG ELISAs indicate that the antibodies reactive to gag are specifically affected relative to env reactivity and that different levels of antibodies to separate viral components in these sera may correlate with disease state. Glycosaminoglycans 116-119 endogenous retrovirus group K member 20 Homo sapiens 56-59 3495201-6 1987 The final preparation of gag and gag/env proteins in 8 M urea reacted with sheep anti-HTLV-III p24 gag antibodies and acquired immune deficiency syndrome (AIDS) patient sera. Glycosaminoglycans 33-36 endogenous retrovirus group K member 20 Homo sapiens 37-40 3495201-6 1987 The final preparation of gag and gag/env proteins in 8 M urea reacted with sheep anti-HTLV-III p24 gag antibodies and acquired immune deficiency syndrome (AIDS) patient sera. Glycosaminoglycans 33-36 endogenous retrovirus group K member 20 Homo sapiens 37-40 3484980-6 1986 A minority of those patients positive for salivary antibodies to env gene-encoded gp160 and gp120 also had salivary antibodies to gag gene-encoded proteins of 55,000, 24,000, and/or 17,000 daltons. Glycosaminoglycans 130-133 endogenous retrovirus group K member 20 Homo sapiens 65-68 2989561-7 1985 Although the gag-env deletion endpoints were identical in the two subclones, heterogeneity was observed across the src deletion in that both mutants analyzed had the same 5" endpoint but slightly different 3" endpoints. Glycosaminoglycans 13-16 endogenous retrovirus group K member 20 Homo sapiens 17-20 6275112-4 1981 The sequential appearance of these RNAs, the probable mRNA"s for the gag and env proteins, paralleled the order of appearance of the gag and env proteins, respectively, after hormone treatment. Glycosaminoglycans 69-72 endogenous retrovirus group K member 20 Homo sapiens 141-144 6275112-4 1981 The sequential appearance of these RNAs, the probable mRNA"s for the gag and env proteins, paralleled the order of appearance of the gag and env proteins, respectively, after hormone treatment. Glycosaminoglycans 133-136 endogenous retrovirus group K member 20 Homo sapiens 77-80 32820216-7 2020 Notably, RhCMV-Gag and RhCMV-Env vaccines elicited anti-Gag and anti-Env antibodies in RhCMV-seronegative RM, an unexpected contrast to vaccination of RhCMV-seropositive RM. Glycosaminoglycans 15-18 endogenous retrovirus group K member 20 Homo sapiens 69-72 32616241-6 2020 The results of entropy analysis of the sequences of gag, pol, and env revealed that the env gene had the largest variation, and the gag gene nonconserved sites are mainly concentrated in p19, p10, and p12. Glycosaminoglycans 52-55 endogenous retrovirus group K member 20 Homo sapiens 88-91 32616241-6 2020 The results of entropy analysis of the sequences of gag, pol, and env revealed that the env gene had the largest variation, and the gag gene nonconserved sites are mainly concentrated in p19, p10, and p12. Glycosaminoglycans 132-135 endogenous retrovirus group K member 20 Homo sapiens 66-69 30894475-9 2019 Finally, we demonstrate how our methodology can be adapted to interrogate interactions between membrane-associated Env and the matrix domain of Gag. Glycosaminoglycans 144-147 endogenous retrovirus group K member 20 Homo sapiens 115-118 32429351-5 2020 There is strong evidence that formation of the Gag lattice on the PM is a prerequisite for the incorporation of Env into budding particles. Glycosaminoglycans 47-50 endogenous retrovirus group K member 20 Homo sapiens 112-115 32429351-6 2020 It is also suggested that Env incorporation is mediated by an interaction between its cytoplasmic tail (gp41CT) and the MA domain of Gag. Glycosaminoglycans 133-136 endogenous retrovirus group K member 20 Homo sapiens 26-29 32429351-8 2020 Elucidation of the molecular determinants of Gag-Env-membrane interactions may help in the development of new antiviral therapeutic agents that inhibit particle assembly, Env incorporation and ultimately virus production. Glycosaminoglycans 45-48 endogenous retrovirus group K member 20 Homo sapiens 49-52 32429351-8 2020 Elucidation of the molecular determinants of Gag-Env-membrane interactions may help in the development of new antiviral therapeutic agents that inhibit particle assembly, Env incorporation and ultimately virus production. Glycosaminoglycans 45-48 endogenous retrovirus group K member 20 Homo sapiens 171-174 29748537-5 2018 We postulate that this neck-biased distribution is regulated by vesicular retention and steric complementarity of Env during independent Gag lattice formation. Glycosaminoglycans 137-140 endogenous retrovirus group K member 20 Homo sapiens 114-117 26711999-1 2016 The matrix (MA) domain of HIV Gag has important functions in directing the trafficking of Gag to sites of assembly and mediating the incorporation of the envelope glycoprotein (Env) into assembling particles. Glycosaminoglycans 30-33 endogenous retrovirus group K member 20 Homo sapiens 154-175 28916807-5 2017 This mobility increase is dependent on Gag-interacting Env tail but not on changes in viral envelope lipid order. Glycosaminoglycans 39-42 endogenous retrovirus group K member 20 Homo sapiens 55-58 27549192-2 2016 The FV Gag N-terminal region is responsible for capsid formation and particle budding via interaction with Env. Glycosaminoglycans 7-10 endogenous retrovirus group K member 20 Homo sapiens 107-110 27549192-4 2016 RESULTS: Mutagenesis of N-terminal Gag residues of feline FV (FFV) reveals key residues essential for either capsid assembly and/or viral budding via interaction with the FFV Env leader protein (Elp). Glycosaminoglycans 35-38 endogenous retrovirus group K member 20 Homo sapiens 175-178 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 179-182 endogenous retrovirus group K member 20 Homo sapiens 97-100 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 179-182 endogenous retrovirus group K member 20 Homo sapiens 167-170 26656715-9 2015 Together, these data suggest that robust Gag-specific CD4(+) T cells and, to a lesser extent, gp41-specific CD4(+) T cells may provide important intermolecular help to Env-specific B cells that promote the generation or maintenance of Env-specific neutralizing antibodies. Glycosaminoglycans 41-44 endogenous retrovirus group K member 20 Homo sapiens 168-171 26656715-9 2015 Together, these data suggest that robust Gag-specific CD4(+) T cells and, to a lesser extent, gp41-specific CD4(+) T cells may provide important intermolecular help to Env-specific B cells that promote the generation or maintenance of Env-specific neutralizing antibodies. Glycosaminoglycans 41-44 endogenous retrovirus group K member 20 Homo sapiens 235-238 26623642-8 2015 The ratio of non-synonymous to synonymous substitutions in the env gene (0.7-0.75) was higher than the gag (0.26-0.34) or pol (0.21-0.26) genes. Glycosaminoglycans 103-106 endogenous retrovirus group K member 20 Homo sapiens 63-66 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 179-182 endogenous retrovirus group K member 20 Homo sapiens 167-170 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 179-182 endogenous retrovirus group K member 20 Homo sapiens 167-170 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 256-259 endogenous retrovirus group K member 20 Homo sapiens 167-170 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 256-259 endogenous retrovirus group K member 20 Homo sapiens 167-170 27170746-8 2016 Combined, these results suggest a stepwise model for viral cell-to-cell transmission wherein (i) Env-receptor interactions anchor target cells to infected cells, (ii) Env signals Gag"s recruitment to the cell-cell contact dependent on an intact Env CT and Gag MA, and (iii) Env CT and Gag MA, in conjunction with extracellular forces, combine to regulate VS stability and infectious outcomes. Glycosaminoglycans 256-259 endogenous retrovirus group K member 20 Homo sapiens 167-170 27128502-9 2016 In accordance with previous findings, isolated Env FTR was associated with higher plasma HIV RNA and lower CD4 counts, while patients with both Gag and Env FTR also had higher Gag- and Env-specific proliferative CD8+ T cell responses. Glycosaminoglycans 176-179 endogenous retrovirus group K member 20 Homo sapiens 152-155 27128502-9 2016 In accordance with previous findings, isolated Env FTR was associated with higher plasma HIV RNA and lower CD4 counts, while patients with both Gag and Env FTR also had higher Gag- and Env-specific proliferative CD8+ T cell responses. Glycosaminoglycans 176-179 endogenous retrovirus group K member 20 Homo sapiens 152-155 26711999-1 2016 The matrix (MA) domain of HIV Gag has important functions in directing the trafficking of Gag to sites of assembly and mediating the incorporation of the envelope glycoprotein (Env) into assembling particles. Glycosaminoglycans 30-33 endogenous retrovirus group K member 20 Homo sapiens 177-180 26537682-5 2015 We found that Gag expression from integrated proviruses occurred in resting cells that lacked surface CD4, likely resulting from Nef- and Env-mediated receptor internalization. Glycosaminoglycans 14-17 endogenous retrovirus group K member 20 Homo sapiens 138-141 25360552-7 2014 Single genome amplification characterized the infections of 2 unprotected animals in the gag-env immunized group, both of which had reduced acute plasma viral loads that ended as transient infections indicating partial immune control. Glycosaminoglycans 89-92 endogenous retrovirus group K member 20 Homo sapiens 93-96 26125521-0 2015 Dose-dependent inhibition of Gag cellular immunity by Env in SIV/HIV DNA vaccinated macaques. Glycosaminoglycans 29-32 endogenous retrovirus group K member 20 Homo sapiens 54-57 26125521-5 2015 In contrast, under high env:gag DNA plasmid ratio, the development of Gag cellular responses was significantly reduced by either SIV or HIV Env, whereas Gag humoral responses were not affected. Glycosaminoglycans 70-73 endogenous retrovirus group K member 20 Homo sapiens 24-27 26125521-5 2015 In contrast, under high env:gag DNA plasmid ratio, the development of Gag cellular responses was significantly reduced by either SIV or HIV Env, whereas Gag humoral responses were not affected. Glycosaminoglycans 70-73 endogenous retrovirus group K member 20 Homo sapiens 140-143 26342234-2 2015 Although, the precise mechanism remains elusive, interaction between Env and the matrix (MA) domain of Gag plays a central role. Glycosaminoglycans 103-106 endogenous retrovirus group K member 20 Homo sapiens 69-72 25360552-8 2014 Four of 6 rhesus were infected in the gag-env + GM-CSF group which demonstrated that GM-CSF abrogated protection. Glycosaminoglycans 38-41 endogenous retrovirus group K member 20 Homo sapiens 42-45 25360552-12 2014 Because there were only 2 infected animals in the gag-env vaccinated rhesus compared to 10 infected rhesus in the other 2 groups, the significance of finding single env variants in the gag-env vaccinated group could not be established. Glycosaminoglycans 50-53 endogenous retrovirus group K member 20 Homo sapiens 54-57 24741089-8 2014 Interestingly the induction of Gag-specific CD4 T cell responses in acute HIV infection was significantly inversely correlated with viral set point in chronic HIV infection (R = -0.5; P = 0.03), while the cumulative contribution of Env-specific CD4 T cell responses showed the reverse effect. Glycosaminoglycans 31-34 endogenous retrovirus group K member 20 Homo sapiens 232-235 23637402-5 2013 We found that Gag assembly induced the aggregation of small Env clusters into larger domains and that these domains were completely immobile. Glycosaminoglycans 14-17 endogenous retrovirus group K member 20 Homo sapiens 60-63 23637402-6 2013 Truncation of the cytoplasmic tail (CT) of Env abrogated Gag"s ability to induce Env clustering and restored Env mobility at assembly sites, both of which correlated with increased Env-induced fusion of infected and uninfected cells. Glycosaminoglycans 57-60 endogenous retrovirus group K member 20 Homo sapiens 43-46 23637402-6 2013 Truncation of the cytoplasmic tail (CT) of Env abrogated Gag"s ability to induce Env clustering and restored Env mobility at assembly sites, both of which correlated with increased Env-induced fusion of infected and uninfected cells. Glycosaminoglycans 57-60 endogenous retrovirus group K member 20 Homo sapiens 81-84 23637402-6 2013 Truncation of the cytoplasmic tail (CT) of Env abrogated Gag"s ability to induce Env clustering and restored Env mobility at assembly sites, both of which correlated with increased Env-induced fusion of infected and uninfected cells. Glycosaminoglycans 57-60 endogenous retrovirus group K member 20 Homo sapiens 81-84 23637402-6 2013 Truncation of the cytoplasmic tail (CT) of Env abrogated Gag"s ability to induce Env clustering and restored Env mobility at assembly sites, both of which correlated with increased Env-induced fusion of infected and uninfected cells. Glycosaminoglycans 57-60 endogenous retrovirus group K member 20 Homo sapiens 81-84 23637402-7 2013 Hence, while Env trapping by Gag secures Env incorporation into viral particles, Env clustering and its sequestration at assembly sites likely also leads to the repression of its fusion function, and thus, by preventing the formation of syncytia, Gag helps to secure efficient transfer of viral particles to target cells. Glycosaminoglycans 29-32 endogenous retrovirus group K member 20 Homo sapiens 13-16 23637402-7 2013 Hence, while Env trapping by Gag secures Env incorporation into viral particles, Env clustering and its sequestration at assembly sites likely also leads to the repression of its fusion function, and thus, by preventing the formation of syncytia, Gag helps to secure efficient transfer of viral particles to target cells. Glycosaminoglycans 29-32 endogenous retrovirus group K member 20 Homo sapiens 41-44 23637402-7 2013 Hence, while Env trapping by Gag secures Env incorporation into viral particles, Env clustering and its sequestration at assembly sites likely also leads to the repression of its fusion function, and thus, by preventing the formation of syncytia, Gag helps to secure efficient transfer of viral particles to target cells. Glycosaminoglycans 29-32 endogenous retrovirus group K member 20 Homo sapiens 41-44 23468635-8 2013 The observed Env accumulation surrounding Gag assemblies, with a lower density on the actual bud, could facilitate viral spread in vivo. Glycosaminoglycans 42-45 endogenous retrovirus group K member 20 Homo sapiens 13-16 23705972-4 2013 It has been long established that the gp41-CT interacts with the Gag precursor protein to ensure Env incorporation into the virion. Glycosaminoglycans 65-68 endogenous retrovirus group K member 20 Homo sapiens 97-100 23675305-4 2013 In addition, PFV Gag interacts with the FV Envelope (Env) protein to facilitate budding of infectious particles. Glycosaminoglycans 17-20 endogenous retrovirus group K member 20 Homo sapiens 43-46 23675305-7 2013 We present the crystal structure of a dimeric amino terminal domain from PFV, Gag-NtD, both free and in complex with the leader peptide of PFV Env. Glycosaminoglycans 78-81 endogenous retrovirus group K member 20 Homo sapiens 143-146 23675305-9 2013 Furthermore, we present structural, biochemical and virological data that reveal the molecular details of the essential Gag-Env interaction and in addition we also examine the specificity of Trim5alpha restriction of PFV. Glycosaminoglycans 120-123 endogenous retrovirus group K member 20 Homo sapiens 124-127 23618494-10 2013 Pull-down assays, using proteins of mammalian and prokaryotic origin, support the previous hypothesis of a direct interaction of both PFV proteins without requirement for cellular cofactors and suggest a potential direct contact of Env through this N-terminal Gag domain. Glycosaminoglycans 260-263 endogenous retrovirus group K member 20 Homo sapiens 232-235 23613843-2 2013 In the present study, we found that the loss of virus infectivity as a result of envelope (Env) incorporation defect caused by a Gag matrix (MA) mutation (L30E) was significantly alleviated by introducing a start codon mutation in vpu. Glycosaminoglycans 129-132 endogenous retrovirus group K member 20 Homo sapiens 91-94 23325685-2 2013 Genetic and biochemical data indicate that the matrix (MA) domain of Gag and the cytoplasmic tail of the transmembrane glycoprotein gp41 play an important role in coordinating Env incorporation; however, the molecular mechanism and possible role of host factors in this process remain to be defined. Glycosaminoglycans 69-72 endogenous retrovirus group K member 20 Homo sapiens 176-179 22163342-10 2011 As expected, the addition or insertion of myr-signals that allowed Env-independent budding of FFV SVPs also retargeted Gag to plasma membrane-proximal sites and other intracellular membrane compartments. Glycosaminoglycans 119-122 endogenous retrovirus group K member 20 Homo sapiens 67-70 21736424-6 2012 Our results demonstrated that when the individual Gag, Pol, or Env gene products were coimmunized with the whole repertoire of nonstructural proteins, the Gag-specific CD8(+) T response was greatly enhanced, while the Env- and Pol-specific CD8(+) T responses were significantly reduced. Glycosaminoglycans 50-53 endogenous retrovirus group K member 20 Homo sapiens 218-221 21736424-6 2012 Our results demonstrated that when the individual Gag, Pol, or Env gene products were coimmunized with the whole repertoire of nonstructural proteins, the Gag-specific CD8(+) T response was greatly enhanced, while the Env- and Pol-specific CD8(+) T responses were significantly reduced. Glycosaminoglycans 155-158 endogenous retrovirus group K member 20 Homo sapiens 63-66 22031937-8 2012 Furthermore, Gag/Env ratios were a potent marker of viral control, with a high frequency and magnitude of Gag responses and low proportion of Env responses associated with effective immune control. Glycosaminoglycans 13-16 endogenous retrovirus group K member 20 Homo sapiens 142-145 22031937-8 2012 Furthermore, Gag/Env ratios were a potent marker of viral control, with a high frequency and magnitude of Gag responses and low proportion of Env responses associated with effective immune control. Glycosaminoglycans 106-109 endogenous retrovirus group K member 20 Homo sapiens 17-20 22811910-1 2012 Together with the Gag protein, the Env glycoprotein is a major retroviral structural protein and is essential for forming infectious virus particles. Glycosaminoglycans 18-21 endogenous retrovirus group K member 20 Homo sapiens 35-38