PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 22547569-8 2012 Conversely, proteolysis of 5alpha-dihydrotestosterone (DHT)-bound SHBG was similar for both KLKs and left the steroid binding LG4 domain intact. Dihydrotestosterone 27-53 sex hormone binding globulin Homo sapiens 66-70 22936694-7 2012 We also observed that the SHBG locus was associated with serum DHT levels (rs727428: P = 1.47 x 10(-11)). Dihydrotestosterone 63-66 sex hormone binding globulin Homo sapiens 26-30 22936694-9 2012 These three loci (JMJD1C, SHBG and FAM9B) were estimated to account for ~5.3 and 4.1% of the variance of serum T and DHT levels. Dihydrotestosterone 117-120 sex hormone binding globulin Homo sapiens 26-30 22547569-8 2012 Conversely, proteolysis of 5alpha-dihydrotestosterone (DHT)-bound SHBG was similar for both KLKs and left the steroid binding LG4 domain intact. Dihydrotestosterone 55-58 sex hormone binding globulin Homo sapiens 66-70 22547569-10 2012 Consistent with this, both full-length SHBG and SHBG-LG4 significantly increased DHT-mediated transcriptional activity of the androgen receptor compared with DHT delivered without carrier protein. Dihydrotestosterone 81-84 sex hormone binding globulin Homo sapiens 39-43 22547569-10 2012 Consistent with this, both full-length SHBG and SHBG-LG4 significantly increased DHT-mediated transcriptional activity of the androgen receptor compared with DHT delivered without carrier protein. Dihydrotestosterone 81-84 sex hormone binding globulin Homo sapiens 48-52 21070796-8 2011 These data support the hypothesis that the changes in T and DHT that occur across the cycle may reflect changes in SHBG and estrogen, whereas in menopause, androgen levels decrease. Dihydrotestosterone 60-63 sex hormone binding globulin Homo sapiens 115-119 21193555-2 2011 The main ligands of SHBG, testosterone and its metabolite, 5alpha-dihydrotestosterone (DHT), alter expression of androgen-responsive genes in the kidney. Dihydrotestosterone 59-85 sex hormone binding globulin Homo sapiens 20-24 21193555-2 2011 The main ligands of SHBG, testosterone and its metabolite, 5alpha-dihydrotestosterone (DHT), alter expression of androgen-responsive genes in the kidney. Dihydrotestosterone 87-90 sex hormone binding globulin Homo sapiens 20-24 21193555-7 2011 Transcriptome profiling after testosterone or DHT pretreatments, followed by 3 d of steroid withdrawal, also demonstrated that intracellular SHBG enhances androgen-dependent stimulation (e.g. Adh7, Vcam1, Areg, Tnfaip2) or repression (e.g. Cldn2 and Osr2) of many other genes in PCT cells. Dihydrotestosterone 46-49 sex hormone binding globulin Homo sapiens 141-145 20452803-1 2010 Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that binds with high affinity to sex steroids, most notably 5alpha-dihydrotestosterone, testosterone, and 17beta-estradiol, thereby regulating their bioavailability and access to target cells. Dihydrotestosterone 121-147 sex hormone binding globulin Homo sapiens 0-28 20452803-1 2010 Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that binds with high affinity to sex steroids, most notably 5alpha-dihydrotestosterone, testosterone, and 17beta-estradiol, thereby regulating their bioavailability and access to target cells. Dihydrotestosterone 121-147 sex hormone binding globulin Homo sapiens 30-34 19321131-1 2009 In humans, testosterone (T), dihydrotestosterone (DHT), estradiol (E2), estrone (E1) and cortisol (C) bind to the serum proteins sex hormone-binding globulin (SHBG), albumin (Alb) and corticosteroid-binding globulin (CBG). Dihydrotestosterone 29-48 sex hormone binding globulin Homo sapiens 129-157 19321131-1 2009 In humans, testosterone (T), dihydrotestosterone (DHT), estradiol (E2), estrone (E1) and cortisol (C) bind to the serum proteins sex hormone-binding globulin (SHBG), albumin (Alb) and corticosteroid-binding globulin (CBG). Dihydrotestosterone 29-48 sex hormone binding globulin Homo sapiens 159-163 19321131-1 2009 In humans, testosterone (T), dihydrotestosterone (DHT), estradiol (E2), estrone (E1) and cortisol (C) bind to the serum proteins sex hormone-binding globulin (SHBG), albumin (Alb) and corticosteroid-binding globulin (CBG). Dihydrotestosterone 50-53 sex hormone binding globulin Homo sapiens 129-157 19321131-1 2009 In humans, testosterone (T), dihydrotestosterone (DHT), estradiol (E2), estrone (E1) and cortisol (C) bind to the serum proteins sex hormone-binding globulin (SHBG), albumin (Alb) and corticosteroid-binding globulin (CBG). Dihydrotestosterone 50-53 sex hormone binding globulin Homo sapiens 159-163 17258903-1 2007 SHBG (sex hormone-binding globulin) is a transport protein specific for dihydrotestosterone, testosterone and estradiol. Dihydrotestosterone 72-91 sex hormone binding globulin Homo sapiens 0-4 17258903-1 2007 SHBG (sex hormone-binding globulin) is a transport protein specific for dihydrotestosterone, testosterone and estradiol. Dihydrotestosterone 72-91 sex hormone binding globulin Homo sapiens 6-34 16728719-10 2006 The rapid responses triggered by DHT did not appear to be mediated through classic intracellular androgen receptors, c-Src kinase-androgen receptor complex, or sex hormone-binding globulin but through a G protein-coupled receptor in LNCaP prostate cancer cells. Dihydrotestosterone 33-36 sex hormone binding globulin Homo sapiens 117-188 16163844-6 2005 Phytoestrogens compete with 17beta-estradiol much stronger than with 5alpha-dihydrotestosterone for binding to sex hormone-binding globulin. Dihydrotestosterone 69-95 sex hormone binding globulin Homo sapiens 111-139 16879062-6 2006 In addition, ligand-binding properties of SHBG partially purified from sera of 23 cirrhotic patients and 20 controls were analyzed by a real-time biosensor technique using a surface-coated dihydrotestosterone derivative. Dihydrotestosterone 189-208 sex hormone binding globulin Homo sapiens 42-46 12054810-1 2002 The crystal structure of human sex hormone-binding globulin (SHBG) has revealed how 5alpha-dihydrotestosterone intercalates between the two seven-stranded beta-sheets of its amino-terminal laminin G-like domain. Dihydrotestosterone 84-110 sex hormone binding globulin Homo sapiens 31-59 14622020-1 2003 Binding studies of the interaction of immobilized 1alpha- and 17alpha-aminoalkyl derivatives of 5alpha-dihydrotestosterone (DHT) with purified N-deglycosylated homodimeric human sex hormone-binding globulin (SHBG) were performed using a surface plasmon resonance biosensor. Dihydrotestosterone 96-122 sex hormone binding globulin Homo sapiens 178-206 14622020-1 2003 Binding studies of the interaction of immobilized 1alpha- and 17alpha-aminoalkyl derivatives of 5alpha-dihydrotestosterone (DHT) with purified N-deglycosylated homodimeric human sex hormone-binding globulin (SHBG) were performed using a surface plasmon resonance biosensor. Dihydrotestosterone 96-122 sex hormone binding globulin Homo sapiens 208-212 14622020-1 2003 Binding studies of the interaction of immobilized 1alpha- and 17alpha-aminoalkyl derivatives of 5alpha-dihydrotestosterone (DHT) with purified N-deglycosylated homodimeric human sex hormone-binding globulin (SHBG) were performed using a surface plasmon resonance biosensor. Dihydrotestosterone 124-127 sex hormone binding globulin Homo sapiens 178-206 14622020-2 2003 These 1alpha- and 17alpha-derivatives with spacers of appropriate lengths between the amine function and the steroid ring skeleton enabled privileged, sterically undisturbed, interactions of either the 17- or 3-characteristic functional groups of DHT with SHBG. Dihydrotestosterone 247-250 sex hormone binding globulin Homo sapiens 256-260 14622020-6 2003 This hypothesis of a bivalent binding model was also directly suggested by a dual fluorescent signal observed by the flow cytometry analysis of SHBG immobilized as a hybrid complex binding simultaneously two 1alpha-aminohexyl DHT ligands, one formed by 3, covalently coupled to phycoerythrin-labeled latex microspheres, and the other by the same DHT derivative, coupled to a fluorescein derivative (4). Dihydrotestosterone 226-229 sex hormone binding globulin Homo sapiens 144-148 12957668-1 2003 We report on the syntheses of 1 alpha- and 17 alpha-aminoalkyl dihydrotestosterone (DHT) derivatives and the particularly high binding affinity of the 1 alpha-aminohexyl ligand for human sex hormone-binding globulin (SHBG). Dihydrotestosterone 84-87 sex hormone binding globulin Homo sapiens 187-215 12957668-1 2003 We report on the syntheses of 1 alpha- and 17 alpha-aminoalkyl dihydrotestosterone (DHT) derivatives and the particularly high binding affinity of the 1 alpha-aminohexyl ligand for human sex hormone-binding globulin (SHBG). Dihydrotestosterone 84-87 sex hormone binding globulin Homo sapiens 217-221 12763261-1 2003 In this study we test the postulate that estradiol conjugated to bovine serum albumin (E-BSA) acts via receptors for the steroid-binding protein sex hormone binding globulin (SHBG) by attempting to block E-BSA-stimulated release of oxytocin with two antagonists of SHBG receptor actions: the 5alpha-reduced androgens dihydrotestosterone (DHT) and 3alpha-diol. Dihydrotestosterone 317-336 sex hormone binding globulin Homo sapiens 145-173 12763261-1 2003 In this study we test the postulate that estradiol conjugated to bovine serum albumin (E-BSA) acts via receptors for the steroid-binding protein sex hormone binding globulin (SHBG) by attempting to block E-BSA-stimulated release of oxytocin with two antagonists of SHBG receptor actions: the 5alpha-reduced androgens dihydrotestosterone (DHT) and 3alpha-diol. Dihydrotestosterone 317-336 sex hormone binding globulin Homo sapiens 175-179 12763261-1 2003 In this study we test the postulate that estradiol conjugated to bovine serum albumin (E-BSA) acts via receptors for the steroid-binding protein sex hormone binding globulin (SHBG) by attempting to block E-BSA-stimulated release of oxytocin with two antagonists of SHBG receptor actions: the 5alpha-reduced androgens dihydrotestosterone (DHT) and 3alpha-diol. Dihydrotestosterone 338-341 sex hormone binding globulin Homo sapiens 145-173 12763261-1 2003 In this study we test the postulate that estradiol conjugated to bovine serum albumin (E-BSA) acts via receptors for the steroid-binding protein sex hormone binding globulin (SHBG) by attempting to block E-BSA-stimulated release of oxytocin with two antagonists of SHBG receptor actions: the 5alpha-reduced androgens dihydrotestosterone (DHT) and 3alpha-diol. Dihydrotestosterone 338-341 sex hormone binding globulin Homo sapiens 175-179 12753385-4 2003 Androgens such as testosterone, 5alpha-dihydrotestosterone, and methyltrienolone (R1881, a potent synthetic androgen), reduced intracellular cAMP levels after treatment with SHBG, but hydrocortisone had no effect. Dihydrotestosterone 32-58 sex hormone binding globulin Homo sapiens 174-178 11932266-10 2002 Serum concentrations of LH, FSH, E2, T, and SHBG decreased significantly during DHT treatment. Dihydrotestosterone 80-83 sex hormone binding globulin Homo sapiens 44-48 12054810-1 2002 The crystal structure of human sex hormone-binding globulin (SHBG) has revealed how 5alpha-dihydrotestosterone intercalates between the two seven-stranded beta-sheets of its amino-terminal laminin G-like domain. Dihydrotestosterone 84-110 sex hormone binding globulin Homo sapiens 61-65 11252818-2 2000 The potency of these substances in [3H]dihydrotestosterone ([3H]-DHT) displacement from the recombinant human androgen receptor (rhAR) and from human sex-hormone binding globulin (hSHBG) was evaluated. Dihydrotestosterone 65-68 sex hormone binding globulin Homo sapiens 150-178 10997612-1 2000 Sex hormone-binding globulin (SHBG) is a plasma glycoprotein with high binding affinity for testosterone and dihydrotestosterone and lower affinity for estradiol. Dihydrotestosterone 109-128 sex hormone binding globulin Homo sapiens 0-28 10997612-1 2000 Sex hormone-binding globulin (SHBG) is a plasma glycoprotein with high binding affinity for testosterone and dihydrotestosterone and lower affinity for estradiol. Dihydrotestosterone 109-128 sex hormone binding globulin Homo sapiens 30-34 10666590-1 1999 The amino-teminal laminin G-like domain of human sex hormone-binding globulin (SHBG), which contains the steroid-binding site and the dimerization domain, has been produced in Escherichia coli, purified to homogeneity and crystallized in complex with 5alpha--dihydrotestosterone (DHT) in two different crystal forms. Dihydrotestosterone 251-278 sex hormone binding globulin Homo sapiens 49-77 10675319-3 2000 The crystal structure of the N-terminal G domain of SHBG in complex with 5alpha-dihydrotestosterone at 1.55 A resolution reveals both the architecture of the steroid-binding site and the quaternary structure of the dimer. Dihydrotestosterone 73-99 sex hormone binding globulin Homo sapiens 52-56 10666590-1 1999 The amino-teminal laminin G-like domain of human sex hormone-binding globulin (SHBG), which contains the steroid-binding site and the dimerization domain, has been produced in Escherichia coli, purified to homogeneity and crystallized in complex with 5alpha--dihydrotestosterone (DHT) in two different crystal forms. Dihydrotestosterone 251-278 sex hormone binding globulin Homo sapiens 79-83 10666590-1 1999 The amino-teminal laminin G-like domain of human sex hormone-binding globulin (SHBG), which contains the steroid-binding site and the dimerization domain, has been produced in Escherichia coli, purified to homogeneity and crystallized in complex with 5alpha--dihydrotestosterone (DHT) in two different crystal forms. Dihydrotestosterone 280-283 sex hormone binding globulin Homo sapiens 49-77 10666590-1 1999 The amino-teminal laminin G-like domain of human sex hormone-binding globulin (SHBG), which contains the steroid-binding site and the dimerization domain, has been produced in Escherichia coli, purified to homogeneity and crystallized in complex with 5alpha--dihydrotestosterone (DHT) in two different crystal forms. Dihydrotestosterone 280-283 sex hormone binding globulin Homo sapiens 79-83 9647574-5 1998 Addition of either oestradiol or 5alpha-dihydro-testosterone (DHT) to cultures previously incubated with SHBG in a final molar ratio of 1:10 resulted in a further increase of HCG and cAMP concentrations. Dihydrotestosterone 33-60 sex hormone binding globulin Homo sapiens 105-109 10197075-1 1998 OBJECTIVE: SHBG is a circulating glycoprotein that binds dihydrotestosterone, testosterone and oestradiol with high affinity and low capacity. Dihydrotestosterone 57-76 sex hormone binding globulin Homo sapiens 11-15 10219893-5 1999 It is conceivable that the SHBG/SHBG-R system works as an additional control mechanism which inhibits or amplifies the effects of DHT and estradiol in cells. Dihydrotestosterone 130-133 sex hormone binding globulin Homo sapiens 27-31 10219893-5 1999 It is conceivable that the SHBG/SHBG-R system works as an additional control mechanism which inhibits or amplifies the effects of DHT and estradiol in cells. Dihydrotestosterone 130-133 sex hormone binding globulin Homo sapiens 32-36 10219893-6 1999 In the prostate, it has been suggested that the estradiol-activated SHBG/SHBG-R complex cross-talks with the androgen receptor, and is able to activate AR even in the absence of DHT. Dihydrotestosterone 178-181 sex hormone binding globulin Homo sapiens 68-72 10219893-6 1999 In the prostate, it has been suggested that the estradiol-activated SHBG/SHBG-R complex cross-talks with the androgen receptor, and is able to activate AR even in the absence of DHT. Dihydrotestosterone 178-181 sex hormone binding globulin Homo sapiens 73-77 10072209-5 1998 The inhibitory activity was higher when SHBG was added before or along with estradiol in the cell culture, as well as when the incubation period was elongated, while was dramatically minimized by the presence of dihydrotestosterone. Dihydrotestosterone 212-231 sex hormone binding globulin Homo sapiens 40-44 9647574-5 1998 Addition of either oestradiol or 5alpha-dihydro-testosterone (DHT) to cultures previously incubated with SHBG in a final molar ratio of 1:10 resulted in a further increase of HCG and cAMP concentrations. Dihydrotestosterone 62-65 sex hormone binding globulin Homo sapiens 105-109 9647574-6 1998 This effect was blocked when cultured placental cells were exposed to SHBG that was previously saturated with DHT or when incubated in the presence of steroids only. Dihydrotestosterone 110-113 sex hormone binding globulin Homo sapiens 70-74 9054366-7 1997 In the absence of androgens, estradiol added to prostate tissue, whose RSHBG was occupied by SHBG, reproduced the results seen with dihydrotestosterone. Dihydrotestosterone 132-151 sex hormone binding globulin Homo sapiens 72-76 9461660-0 1998 Lignans interfering with 5 alpha-dihydrotestosterone binding to human sex hormone-binding globulin. Dihydrotestosterone 25-52 sex hormone binding globulin Homo sapiens 70-98 9358204-4 1997 In the presence of a constant concentration of albumin, the increase of SHBG concentrations resulted in a rapid transfer of estradiol from albumin to globulin while the transfer of DHT was moderate. Dihydrotestosterone 181-184 sex hormone binding globulin Homo sapiens 72-76 7804446-1 1994 Sex hormone-binding globulin (SHBG) is a specific steroid hormone-binding protein that plays a role in transporting dihydrotestosterone, testosterone and estradiol-17 beta (E2), altering their concentration in blood and influencing their biological action. Dihydrotestosterone 116-135 sex hormone binding globulin Homo sapiens 0-28 7626510-9 1995 The addition of DHT to cells, after TeBG has been allowed to bind, resulted in increases in cAMP of greater than 4-fold. Dihydrotestosterone 16-19 sex hormone binding globulin Homo sapiens 36-40 7804446-1 1994 Sex hormone-binding globulin (SHBG) is a specific steroid hormone-binding protein that plays a role in transporting dihydrotestosterone, testosterone and estradiol-17 beta (E2), altering their concentration in blood and influencing their biological action. Dihydrotestosterone 116-135 sex hormone binding globulin Homo sapiens 30-34 7525442-1 1994 In this study we examined the possibility that chronic reduction in serum dihydrotestosterone (DHT) attained by pharmacologic inhibition of 5 alpha-reductase modulates serum sex-hormone-binding globulin (SHBG) levels in elderly men. Dihydrotestosterone 74-93 sex hormone binding globulin Homo sapiens 204-208 7947346-16 1994 The affinity of hSHBG for Site I increased 2-fold when it was complexed with 5 alpha-dihydrotestosterone, testosterone or estradiol. Dihydrotestosterone 77-104 sex hormone binding globulin Homo sapiens 16-21 8013348-3 1994 Human SHBG has a higher affinity for steroids and forms a more stable 5 alpha-dihydrotestosterone (DHT) complex (t1/2 = 40 min) than rat epididymal ABP (t1/2 = 5 min) at 0 C. In this study it was found that recombinant wild-type rat ABP/SHBG bound DHT and estradiol with nearly the same affinities as human SHBG, rather than the affinities of rat epididymal ABP. Dihydrotestosterone 70-97 sex hormone binding globulin Homo sapiens 6-10 8013348-3 1994 Human SHBG has a higher affinity for steroids and forms a more stable 5 alpha-dihydrotestosterone (DHT) complex (t1/2 = 40 min) than rat epididymal ABP (t1/2 = 5 min) at 0 C. In this study it was found that recombinant wild-type rat ABP/SHBG bound DHT and estradiol with nearly the same affinities as human SHBG, rather than the affinities of rat epididymal ABP. Dihydrotestosterone 99-102 sex hormone binding globulin Homo sapiens 6-10 8013348-3 1994 Human SHBG has a higher affinity for steroids and forms a more stable 5 alpha-dihydrotestosterone (DHT) complex (t1/2 = 40 min) than rat epididymal ABP (t1/2 = 5 min) at 0 C. In this study it was found that recombinant wild-type rat ABP/SHBG bound DHT and estradiol with nearly the same affinities as human SHBG, rather than the affinities of rat epididymal ABP. Dihydrotestosterone 99-102 sex hormone binding globulin Homo sapiens 237-241 8013348-3 1994 Human SHBG has a higher affinity for steroids and forms a more stable 5 alpha-dihydrotestosterone (DHT) complex (t1/2 = 40 min) than rat epididymal ABP (t1/2 = 5 min) at 0 C. In this study it was found that recombinant wild-type rat ABP/SHBG bound DHT and estradiol with nearly the same affinities as human SHBG, rather than the affinities of rat epididymal ABP. Dihydrotestosterone 248-251 sex hormone binding globulin Homo sapiens 6-10 7515502-8 1994 Conversely, dihydrotestosterone, which blocks the binding of estradiol to SHBG, completely negates the effect of estradiol. Dihydrotestosterone 12-31 sex hormone binding globulin Homo sapiens 74-78 7525442-1 1994 In this study we examined the possibility that chronic reduction in serum dihydrotestosterone (DHT) attained by pharmacologic inhibition of 5 alpha-reductase modulates serum sex-hormone-binding globulin (SHBG) levels in elderly men. Dihydrotestosterone 95-98 sex hormone binding globulin Homo sapiens 204-208 1327074-12 1992 We also measured the association and dissociation rates of dihydrotestosterone-hABP complexes and the Kd/Ka ratio was very close to the value of Kd of the Scatchard analysis. Dihydrotestosterone 59-78 sex hormone binding globulin Homo sapiens 79-83 1493185-5 1992 Treatment with hSHBG did not modify the plasma clearance of endogenous E2, but accelerated the disappearance rate of 5 alpha-dihydrotestosterone (DHT). Dihydrotestosterone 117-144 sex hormone binding globulin Homo sapiens 15-20 1493185-5 1992 Treatment with hSHBG did not modify the plasma clearance of endogenous E2, but accelerated the disappearance rate of 5 alpha-dihydrotestosterone (DHT). Dihydrotestosterone 146-149 sex hormone binding globulin Homo sapiens 15-20 1493185-6 1992 In animals injected with a tracer dose of radioactive steroids, pretreatment with hSHBG increased uterine and oviductal accumulation of E2- but not DHT-associated radioactivity. Dihydrotestosterone 148-151 sex hormone binding globulin Homo sapiens 82-87 1597861-9 1992 With sex steroid binding protein, a human serum binding protein, the pattern of binding affinities is nearly the reverse, with derivatives of Mib, R1881 and MNT having low affinity, and DHT and T, high affinity. Dihydrotestosterone 186-189 sex hormone binding globulin Homo sapiens 5-32 34855305-5 2022 Both human and ursine recombinant SHBG appeared as dimeric proteins in solution, with a single unfolding temperature of ~58 C. The thermal stability of ursine and human SHBG increased 5.4 and 9.5 C, respectively, in presence of dihydrotestosterone (DHT), suggesting a difference in affinity. Dihydrotestosterone 230-249 sex hormone binding globulin Homo sapiens 170-174 1778174-4 1991 CBG regulates the concentration of free cortisol and progesterone, and SHBG regulates the concentration of free testosterone, dihydrotestosterone, and, to a lesser extent, estradiol. Dihydrotestosterone 126-145 sex hormone binding globulin Homo sapiens 71-75 1659894-1 1991 Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that binds a number of circulating steroid hormones (testosterone, dihydrotestosterone and estradiol) with high affinity, thus regulating their free concentration in plasma. Dihydrotestosterone 128-147 sex hormone binding globulin Homo sapiens 0-28 1659894-1 1991 Sex hormone-binding globulin (SHBG) is a plasma glycoprotein that binds a number of circulating steroid hormones (testosterone, dihydrotestosterone and estradiol) with high affinity, thus regulating their free concentration in plasma. Dihydrotestosterone 128-147 sex hormone binding globulin Homo sapiens 30-34 1958578-3 1991 The first part of the study was to determine the effect of SHBG or albumin on the uptake of [3H]DHT in the cells. Dihydrotestosterone 92-99 sex hormone binding globulin Homo sapiens 59-63 1958578-4 1991 In this experiment both SHBG and albumin inhibits the uptake of [3H]DHT into each of the cell lines when studied in vitro. Dihydrotestosterone 64-71 sex hormone binding globulin Homo sapiens 24-28 1958578-7 1991 Further, this stimulation was increased when testosterone was added to the media, however, [3H]thymidine uptake was decreased by high concentrations of dihydrotestosterone (DHT) or if the SHBG was saturated with DHT prior to being added to the media. Dihydrotestosterone 212-215 sex hormone binding globulin Homo sapiens 188-192 2251223-1 1990 The present studies assess the effects of human sex hormone-binding globulin (SHBG) on the conversion of [3H]testosterone (T) into dihydrotestosterone (DHT) in rat ventral prostate gland in vivo using a constant aortic infusion technique. Dihydrotestosterone 131-150 sex hormone binding globulin Homo sapiens 48-76 2251223-1 1990 The present studies assess the effects of human sex hormone-binding globulin (SHBG) on the conversion of [3H]testosterone (T) into dihydrotestosterone (DHT) in rat ventral prostate gland in vivo using a constant aortic infusion technique. Dihydrotestosterone 131-150 sex hormone binding globulin Homo sapiens 78-82 2251223-1 1990 The present studies assess the effects of human sex hormone-binding globulin (SHBG) on the conversion of [3H]testosterone (T) into dihydrotestosterone (DHT) in rat ventral prostate gland in vivo using a constant aortic infusion technique. Dihydrotestosterone 152-155 sex hormone binding globulin Homo sapiens 78-82 1626920-1 1992 A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Dihydrotestosterone 138-141 sex hormone binding globulin Homo sapiens 20-48 1626920-1 1992 A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Dihydrotestosterone 138-141 sex hormone binding globulin Homo sapiens 50-54 1626920-1 1992 A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Dihydrotestosterone 138-141 sex hormone binding globulin Homo sapiens 84-88 2055206-6 1991 Addition of purified human SHBG to the medium reduced the effectiveness of DHT on both phases of the proliferative response in a dose-dependent manner. Dihydrotestosterone 75-78 sex hormone binding globulin Homo sapiens 27-31 2055206-7 1991 These effects of SHBG appeared to be due primarily to the high affinity binding of DHT by SHBG. Dihydrotestosterone 83-86 sex hormone binding globulin Homo sapiens 17-21 2055206-7 1991 These effects of SHBG appeared to be due primarily to the high affinity binding of DHT by SHBG. Dihydrotestosterone 83-86 sex hormone binding globulin Homo sapiens 90-94 2055206-10 1991 The presence of SHBG in the medium also altered the uptake and metabolism of DHT. Dihydrotestosterone 77-80 sex hormone binding globulin Homo sapiens 16-20 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 51-54 sex hormone binding globulin Homo sapiens 30-34 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 51-54 sex hormone binding globulin Homo sapiens 40-44 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 51-54 sex hormone binding globulin Homo sapiens 40-44 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 99-102 sex hormone binding globulin Homo sapiens 40-44 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 99-102 sex hormone binding globulin Homo sapiens 40-44 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 99-102 sex hormone binding globulin Homo sapiens 40-44 2055206-13 1991 However, in medium containing SHBG, the SHBG-bound DHT remained unconjugated; more than 90% of the DHT initially bound to SHBG was present in the medium at the end of the experiment as unconjugated DHT. Dihydrotestosterone 99-102 sex hormone binding globulin Homo sapiens 40-44 2055206-14 1991 Uptake of radiolabeled DHT by cells was also inhibited by SHBG. Dihydrotestosterone 23-26 sex hormone binding globulin Homo sapiens 58-62 1907862-1 1991 In heifers, the specific 5 alpha-dihydrotestosterone (5 alpha-DHT) binding to sex steroid-binding protein (SBP) was 53 nM in midluteal phase and was 20% lower (p less than 0.05) in early luteal phase. Dihydrotestosterone 25-52 sex hormone binding globulin Homo sapiens 78-105 1907862-1 1991 In heifers, the specific 5 alpha-dihydrotestosterone (5 alpha-DHT) binding to sex steroid-binding protein (SBP) was 53 nM in midluteal phase and was 20% lower (p less than 0.05) in early luteal phase. Dihydrotestosterone 54-65 sex hormone binding globulin Homo sapiens 78-105 2268556-2 1990 The dissociation rate constants of the hormone-SHBG complexes were determined to be 0.016 +/- 0.001, 0.056 +/- 0.002, and 0.083 +/- 0.003 s-1 for DHT, T, and E2, respectively, corresponding to half-times of dissociation (t1/2) of 43, 12 and 8.4 s, respectively. Dihydrotestosterone 146-149 sex hormone binding globulin Homo sapiens 47-51 2166070-5 1990 When LNCaP cells were prebound with SHBG, addition of dihydrotestosterone or estradiol resulted in a dose-dependent increase in intracellular cAMP. Dihydrotestosterone 54-73 sex hormone binding globulin Homo sapiens 36-40 2081088-8 1990 These cells secrete transferrin and ABP, and hABP has an affinity for DHT which is different from that of liver-produced SHBG. Dihydrotestosterone 70-73 sex hormone binding globulin Homo sapiens 45-49 2351098-3 1990 ABP steroid-binding (bioactive) activity was measured in testes and epididymal segments using the radiolabeled ligand [3H]dihydrotestosterone (DHT). Dihydrotestosterone 118-141 sex hormone binding globulin Homo sapiens 0-3 2351098-3 1990 ABP steroid-binding (bioactive) activity was measured in testes and epididymal segments using the radiolabeled ligand [3H]dihydrotestosterone (DHT). Dihydrotestosterone 143-146 sex hormone binding globulin Homo sapiens 0-3 2362451-2 1990 Dihydrotestosterone and testosterone are bound with high affinity by SHBG whereas estradiol is bound with a lower affinity. Dihydrotestosterone 0-19 sex hormone binding globulin Homo sapiens 69-73 2362451-11 1990 It was found that 0.9 mol of 2-iodoestradiol was bound per mol of SHBG dimer (93 kDa) at saturation, and that 2-iodoestradiol competed with dihydrotestosterone for the same binding site of SHBG. Dihydrotestosterone 140-159 sex hormone binding globulin Homo sapiens 66-70 2362451-11 1990 It was found that 0.9 mol of 2-iodoestradiol was bound per mol of SHBG dimer (93 kDa) at saturation, and that 2-iodoestradiol competed with dihydrotestosterone for the same binding site of SHBG. Dihydrotestosterone 140-159 sex hormone binding globulin Homo sapiens 189-193 2341389-7 1990 Furthermore, [14C]BA-DHT-labeled SHBG was digested with trypsin, followed by isolation of the released tryptic peptides by reverse-phase high performance liquid chromatography. Dihydrotestosterone 21-24 sex hormone binding globulin Homo sapiens 33-37 2156840-4 1990 Because the probe for the interaction of SHBG with its receptor is 125I-SHBG, we first showed that 125I-SHBG binds [3H]dihydrotestosterone (DHT) at 4 degrees C and 37 degrees C with KD values similar to those published previously for pure radioinert SHBG. Dihydrotestosterone 115-138 sex hormone binding globulin Homo sapiens 41-45 2156840-4 1990 Because the probe for the interaction of SHBG with its receptor is 125I-SHBG, we first showed that 125I-SHBG binds [3H]dihydrotestosterone (DHT) at 4 degrees C and 37 degrees C with KD values similar to those published previously for pure radioinert SHBG. Dihydrotestosterone 140-143 sex hormone binding globulin Homo sapiens 41-45 2156840-4 1990 Because the probe for the interaction of SHBG with its receptor is 125I-SHBG, we first showed that 125I-SHBG binds [3H]dihydrotestosterone (DHT) at 4 degrees C and 37 degrees C with KD values similar to those published previously for pure radioinert SHBG. Dihydrotestosterone 140-143 sex hormone binding globulin Homo sapiens 72-76 2156840-4 1990 Because the probe for the interaction of SHBG with its receptor is 125I-SHBG, we first showed that 125I-SHBG binds [3H]dihydrotestosterone (DHT) at 4 degrees C and 37 degrees C with KD values similar to those published previously for pure radioinert SHBG. Dihydrotestosterone 140-143 sex hormone binding globulin Homo sapiens 72-76 2156840-4 1990 Because the probe for the interaction of SHBG with its receptor is 125I-SHBG, we first showed that 125I-SHBG binds [3H]dihydrotestosterone (DHT) at 4 degrees C and 37 degrees C with KD values similar to those published previously for pure radioinert SHBG. Dihydrotestosterone 140-143 sex hormone binding globulin Homo sapiens 72-76 2156840-5 1990 125I-SHBG could be prevented from binding to its receptor by a variety of steroids whose relative inhibitory activity (dihydrotestosterone much greater than 2-methoxyestradiol greater than testosterone greater than estradiol much greater than methyltrienolone greater than cortisol) was almost identical to their relative ability to bind to SHBG. Dihydrotestosterone 119-138 sex hormone binding globulin Homo sapiens 5-9 2156840-11 1990 However, when unliganded SHBG was prebound to its receptor, it retained its ability to bind [3H] DHT. Dihydrotestosterone 97-100 sex hormone binding globulin Homo sapiens 25-29 34855305-5 2022 Both human and ursine recombinant SHBG appeared as dimeric proteins in solution, with a single unfolding temperature of ~58 C. The thermal stability of ursine and human SHBG increased 5.4 and 9.5 C, respectively, in presence of dihydrotestosterone (DHT), suggesting a difference in affinity. Dihydrotestosterone 251-254 sex hormone binding globulin Homo sapiens 34-38 34855305-5 2022 Both human and ursine recombinant SHBG appeared as dimeric proteins in solution, with a single unfolding temperature of ~58 C. The thermal stability of ursine and human SHBG increased 5.4 and 9.5 C, respectively, in presence of dihydrotestosterone (DHT), suggesting a difference in affinity. Dihydrotestosterone 251-254 sex hormone binding globulin Homo sapiens 170-174 34855305-6 2022 The dissociation constants for (3 H)DHT were determined to 0.21+-0.04 nM for human and 1.32+-0.10 nM for ursine SHBG, confirming a lower affinity of ursine SHBG. Dihydrotestosterone 36-39 sex hormone binding globulin Homo sapiens 112-116 34855305-6 2022 The dissociation constants for (3 H)DHT were determined to 0.21+-0.04 nM for human and 1.32+-0.10 nM for ursine SHBG, confirming a lower affinity of ursine SHBG. Dihydrotestosterone 36-39 sex hormone binding globulin Homo sapiens 156-160 2761258-1 1989 Sex hormone binding globulin (SHBG) is known to interfere in the quantitation of androgen receptors (AR) if dihydrotestosterone (DHT) is used. Dihydrotestosterone 108-127 sex hormone binding globulin Homo sapiens 0-28 2530403-3 1989 TeBG was evaluated by a filter assay measuring [3H]-DHT binding capacity. Dihydrotestosterone 52-55 sex hormone binding globulin Homo sapiens 0-4 2808412-1 1989 The amino acid sequence of the sex steroid-binding protein (SBP or SHBG) of rabbit serum, specific for binding testosterone and 5 alpha-dihydrotestosterone, was determined using a complementary combination of mass spectrometric and Edman degradation techniques. Dihydrotestosterone 128-155 sex hormone binding globulin Homo sapiens 67-71 2702748-2 1989 In this method, SHBG is saturated with 1 mumol of 5 alpha-dihydrotestosterone per liter and the non-protein-bound fraction is subsequently measured in treated and untreated samples by centrifugal ultrafiltration dialysis (CUFD). Dihydrotestosterone 50-77 sex hormone binding globulin Homo sapiens 16-20 2591057-3 1989 Three main observations provide evidence that dihydrotestosterone plays a major role in SHBG regulation. Dihydrotestosterone 46-65 sex hormone binding globulin Homo sapiens 88-92 2591057-8 1989 In normal boys the magnitude of plasma dihydrotestosterone response to human chorionic gonadotrophin correlates with that of SHBG (r = 0.72) more than testosterone does versus SHBG (r = 0.36). Dihydrotestosterone 39-58 sex hormone binding globulin Homo sapiens 125-129 2591057-9 1989 It is concluded that dihydrotestosterone decreases SHBG concentration in plasma of prepubertal boys. Dihydrotestosterone 21-40 sex hormone binding globulin Homo sapiens 51-55 2591057-10 1989 At least part of the observed decrease in SHBG following testosterone administration in earlier reports must have occurred after its conversion to dihydrotestosterone. Dihydrotestosterone 147-166 sex hormone binding globulin Homo sapiens 42-46 2916378-1 1989 The effect of non-esterified fatty acids (NEFA) on the in vitro binding of testosterone, 5-alpha dihydrotestosterone and estradiol E2 to sex hormone binding globulin (SHBG) was examined using pooled normal female serum, and SHBG and albumin fractions obtained from the partial purification of late pregnancy serum. Dihydrotestosterone 89-116 sex hormone binding globulin Homo sapiens 137-165 2916378-1 1989 The effect of non-esterified fatty acids (NEFA) on the in vitro binding of testosterone, 5-alpha dihydrotestosterone and estradiol E2 to sex hormone binding globulin (SHBG) was examined using pooled normal female serum, and SHBG and albumin fractions obtained from the partial purification of late pregnancy serum. Dihydrotestosterone 89-116 sex hormone binding globulin Homo sapiens 167-171 2916378-6 1989 With partially purified SHBG, the same concentrations of NEFA were less effective at inhibiting the binding of dihydrotestosterone and testosterone but elicited the same effect with E2. Dihydrotestosterone 111-130 sex hormone binding globulin Homo sapiens 24-28 2761258-1 1989 Sex hormone binding globulin (SHBG) is known to interfere in the quantitation of androgen receptors (AR) if dihydrotestosterone (DHT) is used. Dihydrotestosterone 108-127 sex hormone binding globulin Homo sapiens 30-34 2761258-1 1989 Sex hormone binding globulin (SHBG) is known to interfere in the quantitation of androgen receptors (AR) if dihydrotestosterone (DHT) is used. Dihydrotestosterone 129-132 sex hormone binding globulin Homo sapiens 0-28 2761258-1 1989 Sex hormone binding globulin (SHBG) is known to interfere in the quantitation of androgen receptors (AR) if dihydrotestosterone (DHT) is used. Dihydrotestosterone 129-132 sex hormone binding globulin Homo sapiens 30-34 2761258-3 1989 In cytosol of benign prostatic hyperplastic (BPH) tissue low capacity binding for DHT, but not for R1881, was found after removal of SHBG. Dihydrotestosterone 82-85 sex hormone binding globulin Homo sapiens 133-137 2457134-2 1988 Two most frequently used ligands are [3H]dihydrotestosterone [( 3H]DHT) and [3H]methyltrienolone [( 3H]R1881), which in addition to binding to AR also bind to sex hormone binding globulin (SHBG; Kd = 1.5 nM) and progesterone receptors (PgR; Human Kd = 1 nM, rat Kd = 6 nM) respectively. Dihydrotestosterone 37-60 sex hormone binding globulin Homo sapiens 159-187 2439321-6 1987 As expected, both E2 and 5 alpha-dihydrotestosterone bound to human sex hormone-binding globulin, whereas none of the E2 esters bound to this human plasma protein. Dihydrotestosterone 25-52 sex hormone binding globulin Homo sapiens 68-96 3680477-5 1987 The affinity constants of association of the SHBG-DHT complex were similar in hypopituitary and normal boys. Dihydrotestosterone 50-53 sex hormone binding globulin Homo sapiens 45-49 3434794-1 1987 An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. Dihydrotestosterone 218-237 sex hormone binding globulin Homo sapiens 65-89 3434794-1 1987 An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. Dihydrotestosterone 218-237 sex hormone binding globulin Homo sapiens 91-94 3434794-1 1987 An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. Dihydrotestosterone 218-237 sex hormone binding globulin Homo sapiens 247-250 3434794-1 1987 An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. Dihydrotestosterone 239-242 sex hormone binding globulin Homo sapiens 65-89 3434794-1 1987 An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. Dihydrotestosterone 239-242 sex hormone binding globulin Homo sapiens 91-94 3434794-1 1987 An easy, rapid, and sensitive assay that permits measurements of androgen-binding protein (ABP) in tissue as well as in spent media from Sertoli cells is described; this method involves the specific binding of labeled dihydrotestosterone (DHT) to ABP. Dihydrotestosterone 239-242 sex hormone binding globulin Homo sapiens 247-250 3316365-3 1987 Despite their consensual behavior, the correlation coefficient between 5 alpha-dihydrotestosterone and sex hormone-binding globulin was not significant in the obese while in controls it was 0.68 (p less than 0.01). Dihydrotestosterone 71-98 sex hormone binding globulin Homo sapiens 103-131 3586670-2 1987 In individual serum samples, the sex hormone-binding globulin (SHBG) binding capacity for dihydrotestosterone (DHT) was systematically found to be decreased by 30-60% when either gelatin or dithiothreitol (DTT) was present in the assay buffer. Dihydrotestosterone 90-109 sex hormone binding globulin Homo sapiens 33-61 3029159-1 1987 The secretion of sex hormone-binding globulin (SHBG) by the human hepatocarcinoma cell line Hep G2 was increased significantly not only by estradiol (E2) but also by testosterone (T), dihydrotestosterone, and the synthetic androgen danazol in the presence, but not the absence, of fetal calf serum. Dihydrotestosterone 184-203 sex hormone binding globulin Homo sapiens 17-45 3029159-1 1987 The secretion of sex hormone-binding globulin (SHBG) by the human hepatocarcinoma cell line Hep G2 was increased significantly not only by estradiol (E2) but also by testosterone (T), dihydrotestosterone, and the synthetic androgen danazol in the presence, but not the absence, of fetal calf serum. Dihydrotestosterone 184-203 sex hormone binding globulin Homo sapiens 47-51 3568404-7 1987 A careful analysis of experimental data, by setting equations specific to this DHT-TeBG system permitted the systematic errors to be corrected and allowed a precise determination of the serum TeBG concentration (27.3 +/- 6.0 nmol/l for 30 healthy men; 40 +/- 13 nmol/l for 15 healthy women). Dihydrotestosterone 79-82 sex hormone binding globulin Homo sapiens 192-196 3598079-4 1987 Sex hormone binding globulin was evaluated using a saturation method (dihydrotestosterone- 3H 0.5-25 nM) with and without 200x cold dihydrotestosterone. Dihydrotestosterone 70-89 sex hormone binding globulin Homo sapiens 0-28 3598079-4 1987 Sex hormone binding globulin was evaluated using a saturation method (dihydrotestosterone- 3H 0.5-25 nM) with and without 200x cold dihydrotestosterone. Dihydrotestosterone 132-151 sex hormone binding globulin Homo sapiens 0-28 3586670-2 1987 In individual serum samples, the sex hormone-binding globulin (SHBG) binding capacity for dihydrotestosterone (DHT) was systematically found to be decreased by 30-60% when either gelatin or dithiothreitol (DTT) was present in the assay buffer. Dihydrotestosterone 90-109 sex hormone binding globulin Homo sapiens 63-67 3586670-2 1987 In individual serum samples, the sex hormone-binding globulin (SHBG) binding capacity for dihydrotestosterone (DHT) was systematically found to be decreased by 30-60% when either gelatin or dithiothreitol (DTT) was present in the assay buffer. Dihydrotestosterone 111-114 sex hormone binding globulin Homo sapiens 33-61 3586670-2 1987 In individual serum samples, the sex hormone-binding globulin (SHBG) binding capacity for dihydrotestosterone (DHT) was systematically found to be decreased by 30-60% when either gelatin or dithiothreitol (DTT) was present in the assay buffer. Dihydrotestosterone 111-114 sex hormone binding globulin Homo sapiens 63-67 3586672-1 1987 Polyacrylamide gel electrophoresis of plasma and concentrated cerebrospinal fluid (CSF) preincubated with tritium labelled 5 alpha-dihydrotestosterone (DHT) showed identical migration of the radioactivity, indicating the presence of sex-hormone-binding globulin (SHBG) in human CSF. Dihydrotestosterone 152-155 sex hormone binding globulin Homo sapiens 263-267 3722336-1 1986 Serum Sex hormone-binding globulin (SHBG) levels and affinity constant (Ka) of SHBG-dihydrotestosterone association were determined in 91 boys, aged 3 months to 15 yr, all at Tanner stage I of pubertal development. Dihydrotestosterone 84-103 sex hormone binding globulin Homo sapiens 79-83 3702439-0 1986 The temperature dependence of the binding of 5 alpha-dihydrotestosterone, testosterone and estradiol to the sex hormone globulin (SHBG) of human plasma. Dihydrotestosterone 45-72 sex hormone binding globulin Homo sapiens 130-134 3702439-1 1986 The binding of 5 alpha-dihydrotestosterone (DHT), testosterone and estradiol to the sex hormone binding globulin (SHBG) and albumin in human plasma has been studied at 4, 20 and 37 degrees C using the method of equilibrium partition in an aqueous two-phase system based on dextran, poly(ethylene glycol) and water. Dihydrotestosterone 15-42 sex hormone binding globulin Homo sapiens 84-112 3702439-1 1986 The binding of 5 alpha-dihydrotestosterone (DHT), testosterone and estradiol to the sex hormone binding globulin (SHBG) and albumin in human plasma has been studied at 4, 20 and 37 degrees C using the method of equilibrium partition in an aqueous two-phase system based on dextran, poly(ethylene glycol) and water. Dihydrotestosterone 15-42 sex hormone binding globulin Homo sapiens 114-118 3702439-1 1986 The binding of 5 alpha-dihydrotestosterone (DHT), testosterone and estradiol to the sex hormone binding globulin (SHBG) and albumin in human plasma has been studied at 4, 20 and 37 degrees C using the method of equilibrium partition in an aqueous two-phase system based on dextran, poly(ethylene glycol) and water. Dihydrotestosterone 44-47 sex hormone binding globulin Homo sapiens 84-112 3702439-1 1986 The binding of 5 alpha-dihydrotestosterone (DHT), testosterone and estradiol to the sex hormone binding globulin (SHBG) and albumin in human plasma has been studied at 4, 20 and 37 degrees C using the method of equilibrium partition in an aqueous two-phase system based on dextran, poly(ethylene glycol) and water. Dihydrotestosterone 44-47 sex hormone binding globulin Homo sapiens 114-118 3702439-3 1986 The affinity of SHBG for DHT is 1.2-1.3 times higher than that for testosterone and 4 times higher than that for estradiol. Dihydrotestosterone 25-28 sex hormone binding globulin Homo sapiens 16-20 3702439-5 1986 The mean values of the free energy of binding, delta G degree, in the temperature range used are -52.3, -51.7 and -48.9 kJ X mol-1 for the binding of DHT, testosterone and estradiol, respectively, to SHBG. Dihydrotestosterone 150-153 sex hormone binding globulin Homo sapiens 200-204 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 63-90 sex hormone binding globulin Homo sapiens 24-52 4038881-4 1985 Sex hormone binding globulin (SHBG) was measured as [3H]-dihydrotestosterone binding capacity. Dihydrotestosterone 57-76 sex hormone binding globulin Homo sapiens 0-28 4038881-4 1985 Sex hormone binding globulin (SHBG) was measured as [3H]-dihydrotestosterone binding capacity. Dihydrotestosterone 57-76 sex hormone binding globulin Homo sapiens 30-34 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 63-90 sex hormone binding globulin Homo sapiens 54-58 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 92-95 sex hormone binding globulin Homo sapiens 24-52 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 92-95 sex hormone binding globulin Homo sapiens 54-58 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 188-191 sex hormone binding globulin Homo sapiens 24-52 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 188-191 sex hormone binding globulin Homo sapiens 54-58 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 188-191 sex hormone binding globulin Homo sapiens 24-52 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 188-191 sex hormone binding globulin Homo sapiens 54-58 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 188-191 sex hormone binding globulin Homo sapiens 24-52 4062231-1 1985 Accurate measurement of sex hormone binding globulin (SHBG) by 5 alpha-dihydrotestosterone (DHT) binding assays requires the establishment of acceptable upper and lower limits of labelled DHT binding and the use of optimal quantities of radioinert DHT to displace labelled DHT. Dihydrotestosterone 188-191 sex hormone binding globulin Homo sapiens 54-58 4062231-2 1985 A computer assisted binding assay for SHBG is described in which upper and lower binding limits are derived mathematically from analysis of labelled DHT binding to eight concentrations of SHBG in the range 25 to 400 nM. Dihydrotestosterone 149-152 sex hormone binding globulin Homo sapiens 38-42 4062231-2 1985 A computer assisted binding assay for SHBG is described in which upper and lower binding limits are derived mathematically from analysis of labelled DHT binding to eight concentrations of SHBG in the range 25 to 400 nM. Dihydrotestosterone 149-152 sex hormone binding globulin Homo sapiens 188-192 4062231-3 1985 The optimal quantity of radioinert DHT for a given SHBG concentration is also derived from these binding curves. Dihydrotestosterone 35-38 sex hormone binding globulin Homo sapiens 51-55 4038570-6 1985 Anti-hTeBG was titrated using protein A-Sepharose which quantitatively binds IgG and therefore bound [3H]DHT-hTeBG-anti-TeBG complexes. Dihydrotestosterone 105-108 sex hormone binding globulin Homo sapiens 6-10 6538212-10 1984 Therefore, it appears that there are at least two androgen binding components in human term placental cytosol, demonstrable after removal of TeBG contamination, that can be shown to have different affinities for DHT and, probably, separate Pe"s. Dihydrotestosterone 212-215 sex hormone binding globulin Homo sapiens 141-145 6541007-2 1984 After suitable dilution, disintegration and addition of labelled dihydrotestosterone-1,2-3 H or testosterone-1,2-3 H, the SHBG was precipitated by the addition of saturated ammonium sulphate in a final concentration of 42.3%. Dihydrotestosterone 65-84 sex hormone binding globulin Homo sapiens 122-126 6436639-8 1984 DHT infusion also reduced serum TeBG levels equally in old and young men. Dihydrotestosterone 0-3 sex hormone binding globulin Homo sapiens 32-36 6538615-1 1984 Thirty-one women with severe premenstrual syndrome had low sex hormone binding globulin (SHBG) binding capacities 30.2 +/- 9.4 nmol DHT bound/l. Dihydrotestosterone 132-135 sex hormone binding globulin Homo sapiens 59-87 6207132-4 1984 It was found the SHBG lowers the DHT content, whereas stilbestrol and cyproterone acetate in the presence of SHBG tends to increase the amount of 3H-DHT in the nuclei. Dihydrotestosterone 33-36 sex hormone binding globulin Homo sapiens 17-21 6683724-7 1983 SHBG fell in both the T-treated normal men (from 6.5 +/- 1.2 ng dihydrotestosterone bound/ml to 4.3 +/- 0.4; P less than 0.02) and the T-treated Klinefelter"s patients (from 16.4 +/- 2 to 4.3 +/- 0.5; P less than 0.01). Dihydrotestosterone 64-83 sex hormone binding globulin Homo sapiens 0-4 6683603-3 1983 Amniotic fluid SHBG binding capacity was measured by competitive saturation analysis using [3H]-5 alpha-dihydrotestosterone as the labelled ligand, after removal of endogenous steroids with dextran-coated charcoal. Dihydrotestosterone 96-123 sex hormone binding globulin Homo sapiens 15-19 6685421-1 1983 The binding capacity of the sex-hormone-binding globulin (SHBG) was determined by means of a new dihydrotestosterone-binding-capacity test in 73 pregnant women admitted to hospital because of vaginal bleeding between the 6th and 18th gestational week. Dihydrotestosterone 97-116 sex hormone binding globulin Homo sapiens 28-56 6685421-1 1983 The binding capacity of the sex-hormone-binding globulin (SHBG) was determined by means of a new dihydrotestosterone-binding-capacity test in 73 pregnant women admitted to hospital because of vaginal bleeding between the 6th and 18th gestational week. Dihydrotestosterone 97-116 sex hormone binding globulin Homo sapiens 58-62 6419413-4 1983 The results of the present study suggest that levonorgestrel appears to be a better ligand than dihydrotestosterone for measuring SHBG. Dihydrotestosterone 96-115 sex hormone binding globulin Homo sapiens 130-134 6686084-1 1983 The important finding was that sex hormone-binding globulin (SHBG) binds 5 alpha-dihydrotestosterone (DHT) with more efficacy than 17 beta-estradiol in patients with breast cancer and that SHBG binds it with less efficacy than 17 beta-estradiol in normal women. Dihydrotestosterone 102-105 sex hormone binding globulin Homo sapiens 31-59 6686084-1 1983 The important finding was that sex hormone-binding globulin (SHBG) binds 5 alpha-dihydrotestosterone (DHT) with more efficacy than 17 beta-estradiol in patients with breast cancer and that SHBG binds it with less efficacy than 17 beta-estradiol in normal women. Dihydrotestosterone 102-105 sex hormone binding globulin Homo sapiens 61-65 6538615-1 1984 Thirty-one women with severe premenstrual syndrome had low sex hormone binding globulin (SHBG) binding capacities 30.2 +/- 9.4 nmol DHT bound/l. Dihydrotestosterone 132-135 sex hormone binding globulin Homo sapiens 89-93 16725609-1 1981 An androgen binding protein (ABP), which binds 5alpha-dihydrotestosterone with high affinity (Ka=0.3x10(9) M(-1)), has been demonstrated in testicular and epididymal cytosols of 5 young post pubertal bulls (15-17 months old) of the Montbeliarde dairy breed. Dihydrotestosterone 47-73 sex hormone binding globulin Homo sapiens 29-32 6811159-0 1982 Comparison of immunodiffusion and DHT-binding methods for the measurement of sex hormone binding globulin in plasma and serum. Dihydrotestosterone 34-37 sex hormone binding globulin Homo sapiens 77-105 7202083-4 1982 TeBG binding capacity, albumin concentration and the association constants for the binding of testosterone, estradiol and 5 alpha-dihydrotestosterone (DHT) to TeBG and albumin at 37 degrees C. Physiological concentrations of some androgen metabolites reported in the literature were also included in the calculations, namely: DHT, 5-androstene-3 beta, 17 beta-diol (Ae) and 5 alpha-androstane-3 alpha, 17 beta-diol (Aa). Dihydrotestosterone 122-149 sex hormone binding globulin Homo sapiens 159-163 7202083-4 1982 TeBG binding capacity, albumin concentration and the association constants for the binding of testosterone, estradiol and 5 alpha-dihydrotestosterone (DHT) to TeBG and albumin at 37 degrees C. Physiological concentrations of some androgen metabolites reported in the literature were also included in the calculations, namely: DHT, 5-androstene-3 beta, 17 beta-diol (Ae) and 5 alpha-androstane-3 alpha, 17 beta-diol (Aa). Dihydrotestosterone 151-154 sex hormone binding globulin Homo sapiens 159-163 7199552-1 1982 Androgen binding protein in human seminal plasma (SPABP) which has a high affinity-low capacity for dihydrotestosterone (DHT) was measured by saturation-charcoal adsorption technique. Dihydrotestosterone 100-119 sex hormone binding globulin Homo sapiens 0-24 7199552-1 1982 Androgen binding protein in human seminal plasma (SPABP) which has a high affinity-low capacity for dihydrotestosterone (DHT) was measured by saturation-charcoal adsorption technique. Dihydrotestosterone 121-124 sex hormone binding globulin Homo sapiens 0-24 7197283-2 1981 This protein was found to be distinct from serum testosterone-estradiol binding globulin (TEBG) by its isoelectric point, its affinity for dihydrotestosterone (DHT) and by Concanavalin A binding. Dihydrotestosterone 160-163 sex hormone binding globulin Homo sapiens 90-94 7197284-8 1981 The mean SHBG binding capacity was 0.99 +/- 0.05 micrograms dihydrotestosterone bound/dl (normal, 1.0-1.8 micrograms/dl). Dihydrotestosterone 60-79 sex hormone binding globulin Homo sapiens 9-13 7192922-3 1981 The effect of SHBG was largely abolished by heating to 60 degrees C for 1 h and by saturating its binding sites by DHT. Dihydrotestosterone 115-118 sex hormone binding globulin Homo sapiens 14-18 6159037-1 1980 Serum testosterone, 5 alpha-dihydrotestosterone and their binding by sex hormone binding globulin (SHBG) were quantified in 10 patients with prostatic hypertrophy, before and after retropubic prostatectomy, and in an age-matched control group. Dihydrotestosterone 20-47 sex hormone binding globulin Homo sapiens 99-103 7189524-5 1980 3) In group II, a significant decrease of SHBG with age occurred (rSHBG.age = -0.438; P less than 0.35) along with the expected increases of E2 (rE2.age = 0.691; P less than 0.001), T (rT.age = 0.765; P less than 0.001), and DHT (rDHT.age = 0.712; P less than 0.001). Dihydrotestosterone 225-228 sex hormone binding globulin Homo sapiens 42-46 7193146-2 1980 The principle of this method is to saturate the SHBG binding sites with dihydrotestosterone (DHT) and measure the amount of SHBG-bound steroid. Dihydrotestosterone 72-91 sex hormone binding globulin Homo sapiens 48-52 7193146-2 1980 The principle of this method is to saturate the SHBG binding sites with dihydrotestosterone (DHT) and measure the amount of SHBG-bound steroid. Dihydrotestosterone 93-96 sex hormone binding globulin Homo sapiens 48-52 7193146-8 1980 The mean values of SHBG-BC, expressed as microgram DHT bound per 100ml serum, were 1.56 +/- 0.33 (SD) in 6 adult men aged 20 approximately 40 and 2.05 +/- 0.75 in 37 adult women aged 20 approximately 36. Dihydrotestosterone 51-54 sex hormone binding globulin Homo sapiens 19-23 7188684-0 1980 Dynamics of the binding capacity of plasma sex hormone binding globulin (SHBG) for testosterone and dihydrotestosterone during puberty. Dihydrotestosterone 100-119 sex hormone binding globulin Homo sapiens 43-71 7188684-0 1980 Dynamics of the binding capacity of plasma sex hormone binding globulin (SHBG) for testosterone and dihydrotestosterone during puberty. Dihydrotestosterone 100-119 sex hormone binding globulin Homo sapiens 73-77 7188684-4 1980 In both sexes, plasma mean values of SHBG binding capacity both for dihydrotestosterone (DHT) and testosterone (T) were significantly higher in prepubertal subjects, falling during puberty to adult levels. Dihydrotestosterone 68-87 sex hormone binding globulin Homo sapiens 37-41 7188684-4 1980 In both sexes, plasma mean values of SHBG binding capacity both for dihydrotestosterone (DHT) and testosterone (T) were significantly higher in prepubertal subjects, falling during puberty to adult levels. Dihydrotestosterone 89-92 sex hormone binding globulin Homo sapiens 37-41 26697-4 1978 hABP had high affinity (K(d) = 7.8 nM) and low capacity (0.27 pmol/mg protein) for 5alpha-dihydrotestosterone (DHT). Dihydrotestosterone 83-109 sex hormone binding globulin Homo sapiens 0-4 574551-4 1979 The levels of ABP were higher in the breeding season and positively correlated with DHT in RTF and SP. Dihydrotestosterone 84-87 sex hormone binding globulin Homo sapiens 14-17 574551-5 1979 It is concluded that ABP might be responsible for the increase in DHT in the reproductive tract of the ram during the breeding season and that ABP in the SP might serve as a useful marker of Sertoli cell function in the ram. Dihydrotestosterone 66-69 sex hormone binding globulin Homo sapiens 21-24 568820-4 1978 Thirty-eight female patients with hyperthyroidism before treatment had markedly elevated levels of SHBG (x +/- SD: 4.85 +/- 2.4 microgram DHT/100 ml) compared with normal controls (1.50 +/- 0.57; p is less than 0.001). Dihydrotestosterone 138-141 sex hormone binding globulin Homo sapiens 99-103 26697-4 1978 hABP had high affinity (K(d) = 7.8 nM) and low capacity (0.27 pmol/mg protein) for 5alpha-dihydrotestosterone (DHT). Dihydrotestosterone 111-114 sex hormone binding globulin Homo sapiens 0-4 26697-5 1978 Binding affinity of human TeBG for DHT was greater (K(d) = 0.66 nM, binding capacity 0.68 pmol/mg protein). Dihydrotestosterone 35-38 sex hormone binding globulin Homo sapiens 26-30 566661-0 1978 [Role of sex hormone binding globulin (SHBG) in the conversion of testosterone to 5 alpha-dihydrotestosterone and androstenedione in the skin from the suprapubic area in women. Dihydrotestosterone 82-109 sex hormone binding globulin Homo sapiens 9-37 566661-0 1978 [Role of sex hormone binding globulin (SHBG) in the conversion of testosterone to 5 alpha-dihydrotestosterone and androstenedione in the skin from the suprapubic area in women. Dihydrotestosterone 82-109 sex hormone binding globulin Homo sapiens 39-43 104672-3 1978 The relative competitive abilities of three natural steroids, a synthetic androgen and two synthetic estrogens, for binding sites on TeBG occupied by radioactive DHT were compared. Dihydrotestosterone 162-165 sex hormone binding globulin Homo sapiens 133-137 561671-0 1977 Study on the binding of dihydrotestosterone, testosterone and oestradiol with sex hormone binding globulin. Dihydrotestosterone 24-43 sex hormone binding globulin Homo sapiens 78-106 576768-1 1977 Levels of testosterone-oestradiol binding globulin (TeBG) were measured by a precipitation technique in terms of dihydrotestosterone-5alpha (DHT) binding capacity in children, adult males and females, pregnant and hirsute women and women with various trophoblastic disorders. Dihydrotestosterone 113-139 sex hormone binding globulin Homo sapiens 52-56 576768-2 1977 It was found that TeBG levels in children of either sex (1.92 +/- 0.16 mug DHT bound/100 ml serum) approached the normal female range (2.31 +/- 0.11) and were distinctly higher than those of the adult male (1.04 +/- 0.09). Dihydrotestosterone 75-78 sex hormone binding globulin Homo sapiens 18-22 827397-2 1976 SHBG was measured by an improved ammonium sulphate precipitation method using 5 alpha-dihydrotestosterone as ligand. Dihydrotestosterone 78-105 sex hormone binding globulin Homo sapiens 0-4 184406-5 1976 Norgestrel competitively inhibited the binding of dihydrotestosterone to TeBG under in vitro conditions. Dihydrotestosterone 50-69 sex hormone binding globulin Homo sapiens 73-77 561671-10 1977 Since the unbound fractions of T and DHT are believed to be biologically active, and since T and DHT are more sensitive than E2 to SHBG variations, the results obtained further support the hypothesis that SHBG plays an important role in the sex steroids balance. Dihydrotestosterone 37-40 sex hormone binding globulin Homo sapiens 205-209 561671-10 1977 Since the unbound fractions of T and DHT are believed to be biologically active, and since T and DHT are more sensitive than E2 to SHBG variations, the results obtained further support the hypothesis that SHBG plays an important role in the sex steroids balance. Dihydrotestosterone 97-100 sex hormone binding globulin Homo sapiens 205-209 1262450-2 1976 In normal women SHBG capacity (expressed in ng DHT bound per ml of plasma) increased from 23.7 +/- 13.7 (SD) in the 5-8th week period, to 74.5 +/- 20.7 in the 17-20th week period, and to 102 +/- 24 in the 37-40th week period. Dihydrotestosterone 47-50 sex hormone binding globulin Homo sapiens 16-20 33058848-6 2021 RESULTS: In Cox regression models mutually adjusted for T, SHBG, and covariates, each standard deviation increment in DHT (0.23 ng/ml) was associated with a 26% lower risk of hip fracture (adjusted hazard ratio [aHR] 0.74, 95% confidence interval (CI) 0.55-1.00, p=0.049). Dihydrotestosterone 118-121 sex hormone binding globulin Homo sapiens 59-63 32168474-7 2020 In women, we observed positive associations of SHBG with all-cause (HR: 1.54, 95% CI: 1.16-2.04) and with other-cause mortality (HR: 1.86, 95% CI: 1.08-3.20) and for DHT with all-cause mortality (HR: 1.32, 95% CI: 1.00-1.73). Dihydrotestosterone 166-169 sex hormone binding globulin Homo sapiens 47-51 32756601-0 2020 Retraction: SHBG Is an Important Factor in Stemness Induction of Cells by DHT In Vitro and Associated with Poor Clinical Features of Prostate Carcinomas. Dihydrotestosterone 74-77 sex hormone binding globulin Homo sapiens 12-16 27750096-11 2017 The four ligands interacted with 19-23 residues of SHBG and a consistent overlapping of the interacting residues for the four ligands with the residues for the natural ligand, dihydrotestosterone (DHT; 82-91% commonality) was shown. Dihydrotestosterone 176-195 sex hormone binding globulin Homo sapiens 51-55 30149073-7 2018 Serum DHEA correlated inversely with %body fat (r = -0.905, P = 0.002), and DHT positively with SHBG (r = 0.842, P = 0.002). Dihydrotestosterone 76-79 sex hormone binding globulin Homo sapiens 96-100 30149073-10 2018 CONCLUSIONS: Serum DHEA levels were best predicted by %body fat, and serum DHT by SHBG. Dihydrotestosterone 75-78 sex hormone binding globulin Homo sapiens 82-86 29845345-8 2018 Multi-adjusted models revealed an inverse association between baseline SHBG, TT, and DHT levels, and incident T2D (odds ratio (OR) = 0.77, 95% CI [0.62, 0.95], p = 0.02; OR 0.70 [0.57, 0.85], p < 0.001 and OR 0.78 [0.63, 0.96], p = 0.02), respectively. Dihydrotestosterone 85-88 sex hormone binding globulin Homo sapiens 71-75 27750096-12 2017 The overlapping SHBG interacting residues among DHT and the four endocrine disruptors suggested that these compounds have potential for interference and disruption in the steroid binding function. Dihydrotestosterone 48-51 sex hormone binding globulin Homo sapiens 16-20 27719672-8 2016 Docking displays showed interactions of alternate plasticizers with 25-30 amino-acid residues of SHBG; 18-20 amino residues overlapped between the natural ligand, DHT, and the three compounds (commonality of 82-91 %). Dihydrotestosterone 163-166 sex hormone binding globulin Homo sapiens 97-101 27719672-9 2016 The hydrogen-bonding interaction of the amino-acid residue, Asn-82, of SHBG was also present in displays of DHT and all the three alternate phthalates. Dihydrotestosterone 108-111 sex hormone binding globulin Homo sapiens 71-75 26963243-7 2016 Docking complexes of the nine phthalates displayed interactions with 15-31 amino acid residues of SHBG and a commonality of 55-95% interacting residues between natural ligand of SHBG, dihydrotestosterone, and the nine phthalate compounds was observed. Dihydrotestosterone 184-203 sex hormone binding globulin Homo sapiens 98-102 26963243-10 2016 Hence, overlapping of interacting amino acid residues between phthalate compounds and natural ligand, dihydrotestosterone, suggested potential disrupting activity of phthalates in the endocrine homeostasis function of SHBG, with long chain phthalates expected to be more potent than the short chain phthalates. Dihydrotestosterone 102-121 sex hormone binding globulin Homo sapiens 218-222