PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9804763-2 1998 The superoxide-generating NADPH oxidase complex of phagocytic cells is a multicomponent system containing a membrane-bound flavocytochrome b and a small G protein Rac as well as cytosolic factors p67(phox) (phagocyte oxidase), p47(phox), and p40(phox), which translocate to the membrane upon activation. Superoxides 4-14 pleckstrin Homo sapiens 227-230 9642219-2 1998 Superoxide generation by the neutrophil respiratory burst oxidase (NADPH oxidase) can be reconstituted in a cell-free system using flavocytochrome b558 and the cytosolic proteins p47(phox), p67(phox), and Rac. Superoxides 0-10 pleckstrin Homo sapiens 179-182 8939991-9 1996 p47(phox) appears to facilitate or stabilize the interaction of p67(phox) and, possibly, Rac1 with cytochrome b559, and is required for optimal generation of O-2 under physiological conditions. Superoxides 158-161 pleckstrin Homo sapiens 0-3 9624128-1 1998 The superoxide generating NADPH oxidase of phagocytes consists, in resting cells, of a membrane-associated electron transporting flavocytochrome (cytochrome b559) and four cytosolic proteins as follows: p47(phox), p67(phox), p40(phox), and the small GTPase, Rac(1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 203-206 9461621-2 1998 Anionic amphiphiles, such as arachidonate, activate the superoxide-producing phagocyte NADPH oxidase in a cell-free system with human neutrophil membrane, which contains cytochrome b558 comprising gp91(phox) and p22(phox), and three cytosolic proteins: p47(phox) and p67(phox), each harboring two SH3 domains, and the small GTPase Rac. Superoxides 56-66 pleckstrin Homo sapiens 202-206 9461621-2 1998 Anionic amphiphiles, such as arachidonate, activate the superoxide-producing phagocyte NADPH oxidase in a cell-free system with human neutrophil membrane, which contains cytochrome b558 comprising gp91(phox) and p22(phox), and three cytosolic proteins: p47(phox) and p67(phox), each harboring two SH3 domains, and the small GTPase Rac. Superoxides 56-66 pleckstrin Homo sapiens 216-220 9461621-2 1998 Anionic amphiphiles, such as arachidonate, activate the superoxide-producing phagocyte NADPH oxidase in a cell-free system with human neutrophil membrane, which contains cytochrome b558 comprising gp91(phox) and p22(phox), and three cytosolic proteins: p47(phox) and p67(phox), each harboring two SH3 domains, and the small GTPase Rac. Superoxides 56-66 pleckstrin Homo sapiens 253-256 9461621-2 1998 Anionic amphiphiles, such as arachidonate, activate the superoxide-producing phagocyte NADPH oxidase in a cell-free system with human neutrophil membrane, which contains cytochrome b558 comprising gp91(phox) and p22(phox), and three cytosolic proteins: p47(phox) and p67(phox), each harboring two SH3 domains, and the small GTPase Rac. Superoxides 56-66 pleckstrin Homo sapiens 216-220 9461621-2 1998 Anionic amphiphiles, such as arachidonate, activate the superoxide-producing phagocyte NADPH oxidase in a cell-free system with human neutrophil membrane, which contains cytochrome b558 comprising gp91(phox) and p22(phox), and three cytosolic proteins: p47(phox) and p67(phox), each harboring two SH3 domains, and the small GTPase Rac. Superoxides 56-66 pleckstrin Homo sapiens 216-220 7658912-8 1995 These observations suggested that sodium beraprost inhibited fMLP induced superoxide generation of human neutrophils by the inhibition of p47phox phosphorylation and translocation by a Ca2+ dependent mechanism. Superoxides 74-84 pleckstrin Homo sapiens 138-141 8939991-0 1996 The cytosolic component p47(phox) is not a sine qua non participant in the activation of NADPH oxidase but is required for optimal superoxide production. Superoxides 131-141 pleckstrin Homo sapiens 24-27 8939991-1 1996 The superoxide (O-2)-generating NADPH oxidase of phagocytes is a multicomponent complex consisting of a membrane-associated flavocytochrome (cytochrome b559), bearing the NADPH binding site and two redox centers (FAD and heme) and three cytosolic activating components: p47(phox), p67(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 270-273 8939991-1 1996 The superoxide (O-2)-generating NADPH oxidase of phagocytes is a multicomponent complex consisting of a membrane-associated flavocytochrome (cytochrome b559), bearing the NADPH binding site and two redox centers (FAD and heme) and three cytosolic activating components: p47(phox), p67(phox), and the small GTPase Rac (1 or 2). Superoxides 16-19 pleckstrin Homo sapiens 270-273 8663333-2 1996 During activation, cytosolic proteins p47(phox), p67(phox), Rac2, and possibly p40(phox) translocate to the plasma membrane and associate with flavocytochrome b to form the active superoxide-generating system. Superoxides 180-190 pleckstrin Homo sapiens 38-41 8663333-2 1996 During activation, cytosolic proteins p47(phox), p67(phox), Rac2, and possibly p40(phox) translocate to the plasma membrane and associate with flavocytochrome b to form the active superoxide-generating system. Superoxides 180-190 pleckstrin Homo sapiens 42-46 8551399-3 1996 The data indicate that mutations in the p47-phagocyte oxidase component of the reduced nicotinamide adenine dinucleotide phosphate oxidase component do not completely prevent oxidation despite severe defects in superoxide generation. Superoxides 211-221 pleckstrin Homo sapiens 40-43 8798426-5 1996 p67(phox)-(1-246), a truncated form of the protein which eliminates SH3 domains involved in binding to p47(phox), also supports superoxide generation, both in the presence and absence of p47(phox), providing further evidence for p47(phox) independent activity. Superoxides 128-138 pleckstrin Homo sapiens 103-106 8703027-7 1996 A mutant p47(phox) with this substitution is incapable of supporting superoxide production under cell-free activation conditions. Superoxides 69-79 pleckstrin Homo sapiens 9-12 8703027-7 1996 A mutant p47(phox) with this substitution is incapable of supporting superoxide production under cell-free activation conditions. Superoxides 69-79 pleckstrin Homo sapiens 13-17 8028054-10 1994 The increase in quantity of p47 correlated with the increase in superoxide production during sepsis, and thus may be the major contributor to the high activity. Superoxides 64-74 pleckstrin Homo sapiens 28-31 8395827-1 1993 The superoxide-generating NADPH oxidase system in phagocytes consists of membrane-associated cytochrome b558 and three cytosolic components named p67-phox, p47-phox, and rac p21s. Superoxides 4-14 pleckstrin Homo sapiens 156-159 8250232-1 1993 An enzyme-linked immunosorbent assay (ELISA) has been developed using polyclonal antibodies raised against two cytosolic proteins of 47 kDa (p47) and 67 kDa (p67) which behave as activation factors for the superoxide-generating NADPH oxidase of neutrophils at the onset of phagocytosis. Superoxides 206-216 pleckstrin Homo sapiens 141-144 29183727-1 2018 The membrane bound cytochrome b558 composed of gp91-phox and p22-phox proteins, and cytosolic proteins p40-, p47-and p67-phox are important components of superoxide (O2-)-generating system in phagocytes. Superoxides 154-164 pleckstrin Homo sapiens 109-112 8167697-0 1993 Is the IFN-gamma-induced enhancement of superoxide production in CGD-phagocytes caused by increased expression of the p47-phox cytosolic protein. Superoxides 40-50 pleckstrin Homo sapiens 118-121 8167728-5 1993 The presence and activity of the retrovirally encoded p47-phox in the transduced cells is demonstrated and we show that this restores their capacity to generate superoxide. Superoxides 161-171 pleckstrin Homo sapiens 54-57 1313435-11 1992 These data strongly suggest that p47 undergoes a continual cycle of phosphorylation and dephosphorylation throughout the period of O2- release when PMA is the stimulus. Superoxides 131-133 pleckstrin Homo sapiens 33-36 1313435-12 1992 Moreover, we show that antagonists of type 1 and 2A protein phosphatases block dephosphorylation of p47 both in vivo and in vitro, indicating that these enzymes may modulate O2- release under certain circumstances. Superoxides 174-176 pleckstrin Homo sapiens 100-103 1325210-5 1992 The presence and activity of the retrovirally encoded p47-phox in the transduced cells is demonstrated and we show that this restores their capacity to generate superoxide. Superoxides 161-171 pleckstrin Homo sapiens 54-57 1316893-1 1992 The superoxide-generating NADPH oxidase system in phagocytes consists of at least membrane-associated cytochrome b558 and three cytosolic components named SOCI/NCF-3/sigma 1/C1, SOCII/NCF-1/p47-phox, and SO-CIII/NCF-2/p67-phox. Superoxides 4-14 pleckstrin Homo sapiens 190-193 1848559-8 1991 Taken together these results indicate that PKC-dependent phosphorylation of p47-phox correlates with association of p47-phox with the cytoskeleton and with translocation of p47-phox and p67-phox to the plasma membrane, with the ensuing assembly of an active superoxide-generating NADPH-dependent oxidase. Superoxides 258-268 pleckstrin Homo sapiens 76-79 1848559-8 1991 Taken together these results indicate that PKC-dependent phosphorylation of p47-phox correlates with association of p47-phox with the cytoskeleton and with translocation of p47-phox and p67-phox to the plasma membrane, with the ensuing assembly of an active superoxide-generating NADPH-dependent oxidase. Superoxides 258-268 pleckstrin Homo sapiens 116-119 2265254-7 1990 All full-length and C-terminal recombinant p47-phox proteins augmented the superoxide-generating capacity of the cell-free system and were phosphorylated when added to cytosol from normal subjects or from a patient with p47-deficient autosomal CGD. Superoxides 75-85 pleckstrin Homo sapiens 43-46 28601846-6 2018 RESULTS: The impaired superoxide production by patients" neutrophils was associated with a severe deficient expression of the NADPH oxidase catalytic core flavocytochrome-b558 (gp91 phox /NOX2 and p22 phox ), its cytosolic partner p47 phox but not p67 phox . Superoxides 22-32 pleckstrin Homo sapiens 231-234 29743977-4 2018 Stimulation of FPR1 by N-fMLP induces p47phox phosphorylation, which is the crucial event for NADPH oxidase-dependent superoxide production. Superoxides 118-128 pleckstrin Homo sapiens 38-41 29183727-1 2018 The membrane bound cytochrome b558 composed of gp91-phox and p22-phox proteins, and cytosolic proteins p40-, p47-and p67-phox are important components of superoxide (O2-)-generating system in phagocytes. Superoxides 166-168 pleckstrin Homo sapiens 109-112 25699251-1 2015 The superoxide (O( -) 2)-generating NADPH oxidase of phagocytes consists of a membrane component, cytochrome b 558 (a heterodimer of Nox2 and p22 (phox) ), and four cytosolic components, p47 (phox) , p67 (phox) , p40 (phox) , and Rac. Superoxides 4-14 pleckstrin Homo sapiens 187-190 24598074-1 2014 The superoxide-generating NADPH oxidase of phagocytes consists of the membrane-associated cytochrome b 558 (a heterodimer of Nox2 and p22(phox)) and 4 cytosolic components: p47(phox), p67(phox), p40(phox), and the small GTPase, Rac, in complex with RhoGDI. Superoxides 4-14 pleckstrin Homo sapiens 173-176 25056956-0 2014 Arachidonic acid induces direct interaction of the p67(phox)-Rac complex with the phagocyte oxidase Nox2, leading to superoxide production. Superoxides 117-127 pleckstrin Homo sapiens 55-59 25056956-1 2014 The phagocyte NADPH oxidase Nox2, heterodimerized with p22(phox) in the membrane, is dormant in resting cells but becomes activated upon cell stimulation to produce superoxide, a precursor of microbicidal oxidants. Superoxides 165-175 pleckstrin Homo sapiens 59-63 25056956-6 2014 However, even when constitutively active forms of p47(phox) and Rac1 are both expressed in HeLa cells, superoxide production by Nox2 is scarcely induced in the absence of AA. Superoxides 103-113 pleckstrin Homo sapiens 50-53 25056956-6 2014 However, even when constitutively active forms of p47(phox) and Rac1 are both expressed in HeLa cells, superoxide production by Nox2 is scarcely induced in the absence of AA. Superoxides 103-113 pleckstrin Homo sapiens 54-58 25056956-9 2014 p67(phox)-Rac-Nox2 assembly and superoxide production are both abrogated by alanine substitution for Tyr-198, Leu-199, and Val-204 in the p67(phox) activation domain that localizes the C-terminal to the Rac-binding domain. Superoxides 32-42 pleckstrin Homo sapiens 142-146 24970888-0 2014 Molecular insights of p47phox phosphorylation dynamics in the regulation of NADPH oxidase activation and superoxide production. Superoxides 105-115 pleckstrin Homo sapiens 22-25 24511121-7 2014 Thrombospondin-1 also stimulated phosphorylation of p47(phox) (an organizer subunit for nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1/2) and increased production of superoxide, both of which were abrogated by knockdown or antibody blockade of SIRP-alpha. Superoxides 181-191 pleckstrin Homo sapiens 52-61 24504963-1 2014 The superoxide (O2 ( -))-generating NADPH oxidase complex of phagocytes comprises a membrane-imbedded heterodimeric flavocytochrome, known as cytochrome b 558 (consisting of Nox2 and p22 (phox) ) and four cytosolic regulatory proteins, p47 (phox) , p67 (phox) , p40 (phox) , and the small GTPase Rac. Superoxides 4-14 pleckstrin Homo sapiens 236-239 21443430-6 2011 This decrease in superoxide release was accompanied by diminished mRNA expression for subunit p47(phox) of the phagocyte superoxide-generating nicotinamide adenine dinucleotide phosphate-oxidase. Superoxides 17-27 pleckstrin Homo sapiens 94-97 21497059-9 2011 Aripiprazole proved to inhibit the O(2)(-) generation through the cascade of protein kinase C (PKC) activation, intracellular Ca(2+) regulation and NADPH oxidase activation via cytosolic p47(phox) translocation to the plasma/phagosomal membranes. Superoxides 36-40 pleckstrin Homo sapiens 188-191 23569086-8 2013 Western blotting demonstrated that incubation of isolated TAL with AngII increased phosphorylation of p47(phox) at Ser(304), suggesting that AngII stimulates the basolateral Cl channels by increasing NADPH oxidase-dependent superoxide generation. Superoxides 224-234 pleckstrin Homo sapiens 102-105 22442695-7 2012 CONCLUSIONS/SIGNIFICANCE: We conclude that formation of peroxynitrite by a reaction between superoxide anion generated by NADPH oxidase in RVLM on activation by AT1R and NOS II-derived NO leads to a reduction in baroreflex-mediated sympathetic vasomotor tone during experimental TLSE; to be ameliorated by the upregulated BDNF/TrkB signaling via inhibition of p47(phox) phosphorylation. Superoxides 92-108 pleckstrin Homo sapiens 360-363 21443430-6 2011 This decrease in superoxide release was accompanied by diminished mRNA expression for subunit p47(phox) of the phagocyte superoxide-generating nicotinamide adenine dinucleotide phosphate-oxidase. Superoxides 121-131 pleckstrin Homo sapiens 94-97 20708598-2 2011 It becomes active when membrane-bound cytochrome b(558), the redox core, is assembled with cytosolic p47(phox), p67(phox), p40(phox), and rac proteins to produce superoxide, the precursor for generation of toxic reactive oxygen species. Superoxides 162-172 pleckstrin Homo sapiens 101-104 20346917-1 2010 The membrane bound cytochrome b558 composed of large gp91-phox and small p22-phox subunits, and cytosolic proteins p40-, p47- and p67-phox are important components of superoxide (O(2)(-))-generating system in phagocytes and B lymphocytes. Superoxides 179-182 pleckstrin Homo sapiens 121-124 20817944-1 2010 The assembly of cytosolic subunits p47(phox), p67(phox), and p40(phox) with flavocytochrome b(558) at the membrane is required for activating the neutrophil NADPH oxidase that generates superoxide for microbial killing. Superoxides 186-196 pleckstrin Homo sapiens 35-38 20529851-1 2010 The superoxide-generating NADPH oxidase complex of resting phagocytes includes cytochrome b(559), a membrane-associated heterodimer composed of two subunits (Nox2 and p22(phox)), and four cytosolic proteins (p47(phox), p67(phox), Rac, and p40(phox)). Superoxides 4-14 pleckstrin Homo sapiens 171-175 20529851-1 2010 The superoxide-generating NADPH oxidase complex of resting phagocytes includes cytochrome b(559), a membrane-associated heterodimer composed of two subunits (Nox2 and p22(phox)), and four cytosolic proteins (p47(phox), p67(phox), Rac, and p40(phox)). Superoxides 4-14 pleckstrin Homo sapiens 208-211 20529851-1 2010 The superoxide-generating NADPH oxidase complex of resting phagocytes includes cytochrome b(559), a membrane-associated heterodimer composed of two subunits (Nox2 and p22(phox)), and four cytosolic proteins (p47(phox), p67(phox), Rac, and p40(phox)). Superoxides 4-14 pleckstrin Homo sapiens 212-216 20529851-1 2010 The superoxide-generating NADPH oxidase complex of resting phagocytes includes cytochrome b(559), a membrane-associated heterodimer composed of two subunits (Nox2 and p22(phox)), and four cytosolic proteins (p47(phox), p67(phox), Rac, and p40(phox)). Superoxides 4-14 pleckstrin Homo sapiens 212-216 20529851-1 2010 The superoxide-generating NADPH oxidase complex of resting phagocytes includes cytochrome b(559), a membrane-associated heterodimer composed of two subunits (Nox2 and p22(phox)), and four cytosolic proteins (p47(phox), p67(phox), Rac, and p40(phox)). Superoxides 4-14 pleckstrin Homo sapiens 212-216 20346917-1 2010 The membrane bound cytochrome b558 composed of large gp91-phox and small p22-phox subunits, and cytosolic proteins p40-, p47- and p67-phox are important components of superoxide (O(2)(-))-generating system in phagocytes and B lymphocytes. Superoxides 167-177 pleckstrin Homo sapiens 121-124 20375610-2 2009 In activation of the oxidase, the multidomain protein p67(phox)plays a central role: it translocates to the membrane as a ternary complex with p47(phox)and p40(phox), and interacts with the small GTPase Rac to assemble with the membrane-integrated catalytic protein gp91(phox), leading to superoxide production. Superoxides 289-299 pleckstrin Homo sapiens 143-146 20172962-7 2010 Enhanced PKC-beta activation by DHEAS resulted in increased phosphorylation of p47(phox), a crucial component of the active reduced nicotinamide adenine dinucleotide phosphate complex responsible for neutrophil superoxide generation. Superoxides 211-221 pleckstrin Homo sapiens 79-82 19414794-4 2009 We observed impaired O(2)( ) generation by LPS-treated and fMLP-activated IRAK4-deficient PMN that correlated with decreased phosphorylation of p47(phox) and subnormal translocation of p47(phox), p67(phox), Rac2, and gp91(phox)/Nox2 to the membranes indicating that TLR4 signaling to the NOX activation pathway requires IRAK4. Superoxides 21-25 pleckstrin Homo sapiens 144-147 19414794-4 2009 We observed impaired O(2)( ) generation by LPS-treated and fMLP-activated IRAK4-deficient PMN that correlated with decreased phosphorylation of p47(phox) and subnormal translocation of p47(phox), p67(phox), Rac2, and gp91(phox)/Nox2 to the membranes indicating that TLR4 signaling to the NOX activation pathway requires IRAK4. Superoxides 21-25 pleckstrin Homo sapiens 185-188 19084054-5 2009 fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins: p47(phox), p67(phox), and Rac to the cell membrane were suppressed in parallel with the suppression of stimulus-induced superoxide generation. Superoxides 210-220 pleckstrin Homo sapiens 90-93 19129478-1 2009 The assembly of cytosolic p47(phox) and p67(phox) with flavocytochrome b(558) at the membrane is crucial for activating the leukocyte NADPH oxidase that generates superoxide for microbial killing. Superoxides 163-173 pleckstrin Homo sapiens 26-35 19129478-1 2009 The assembly of cytosolic p47(phox) and p67(phox) with flavocytochrome b(558) at the membrane is crucial for activating the leukocyte NADPH oxidase that generates superoxide for microbial killing. Superoxides 163-173 pleckstrin Homo sapiens 30-34 19692703-3 2009 A fifth subunit, p40(phox), plays an important role in phagocytosis-induced superoxide production via a phox homology (PX) domain that binds to phosphatidylinositol 3-phosphate (PtdIns(3)P). Superoxides 76-86 pleckstrin Homo sapiens 21-25 19692703-3 2009 A fifth subunit, p40(phox), plays an important role in phagocytosis-induced superoxide production via a phox homology (PX) domain that binds to phosphatidylinositol 3-phosphate (PtdIns(3)P). Superoxides 76-86 pleckstrin Homo sapiens 104-108 19130504-0 2009 HIV-1 Nef induces p47(phox) phosphorylation leading to a rapid superoxide anion release from the U937 human monoblastic cell line. Superoxides 63-79 pleckstrin Homo sapiens 18-21 18424721-4 2008 Our results show that superoxide levels were significantly increased in a time-dependent manner in blood monocyte-derived macrophages treated with 1 muM As(2)O(3) for 72 h. Concomitantly, As(2)O(3) induced phosphorylation and membrane translocation of the NADPH oxidase subunit p47(phox) and it also increased translocation of Rac1 and p67(phox). Superoxides 22-32 pleckstrin Homo sapiens 278-281 18772329-9 2008 The reduction of O2(-) was linked to both reduced expression of p47 phox of NADPH oxidase and overexpression of extracellular superoxide dismutase. Superoxides 17-19 pleckstrin Homo sapiens 64-67 17583407-4 2007 Although Nox3 complexed with p22(phox) constitutively produce superoxide, the production can be enhanced by supportive proteins. Superoxides 62-72 pleckstrin Homo sapiens 33-37 18160398-6 2008 We further present evidence that the effect of PAK1 on the respiratory burst is mediated through phosphorylation of p47(Phox), and we show that expression of a p47(Phox) (S303D/S304D/S320D) mutant, which mimics phosphorylation by PAK1, induced basal superoxide generation in vivo. Superoxides 250-260 pleckstrin Homo sapiens 160-163 17583407-1 2007 The superoxide-producing phagocyte NADPH oxidase gp91(phox)/Nox2 and the non-phagocytic oxidases Nox1 and Nox3 each form a complex in the membrane with p22(phox), which provides both stabilization and a docking site for organizer proteins. Superoxides 4-14 pleckstrin Homo sapiens 54-58 17583407-1 2007 The superoxide-producing phagocyte NADPH oxidase gp91(phox)/Nox2 and the non-phagocytic oxidases Nox1 and Nox3 each form a complex in the membrane with p22(phox), which provides both stabilization and a docking site for organizer proteins. Superoxides 4-14 pleckstrin Homo sapiens 156-160 17942113-10 2007 Furthermore, CRP-induced O(2)(-) production was reversed by pharmacologic inhibition and siRNAs to p47 phox and p22 phox. Superoxides 25-32 pleckstrin Homo sapiens 99-102 17583407-7 2007 When these oxidases use Noxo1 as an organizer instead of p47(phox), they produce a small but significant amount of superoxide without expression of Rac1(Q61L), although the production is enhanced by Rac1(Q61L). Superoxides 115-125 pleckstrin Homo sapiens 57-60 17583407-7 2007 When these oxidases use Noxo1 as an organizer instead of p47(phox), they produce a small but significant amount of superoxide without expression of Rac1(Q61L), although the production is enhanced by Rac1(Q61L). Superoxides 115-125 pleckstrin Homo sapiens 61-65 17583407-10 2007 We also demonstrate that, in the Nox3-based oxidase containing solely p67(phox) as supportive protein, expression of Rac1(Q61L) enhances not only superoxide production but also membrane translocation of p67(phox). Superoxides 146-156 pleckstrin Homo sapiens 74-78 17592079-10 2007 Small-interfering RNA silencing of NAD(P)H oxidase subunit p47(phox) reduced superoxide production and restored NO bioavailability and in vivo reendothelialization capacity of EPCs from diabetic patients. Superoxides 77-87 pleckstrin Homo sapiens 59-62 17548354-1 2007 The superoxide-generating NADPH oxidase is converted to an active state by the assembly of a membrane-localized cytochrome b(559) with three cytosolic components: p47(phox), p67(phox), and GTPase Rac1 or Rac2. Superoxides 4-14 pleckstrin Homo sapiens 163-166 17548354-1 2007 The superoxide-generating NADPH oxidase is converted to an active state by the assembly of a membrane-localized cytochrome b(559) with three cytosolic components: p47(phox), p67(phox), and GTPase Rac1 or Rac2. Superoxides 4-14 pleckstrin Homo sapiens 167-171 17548354-1 2007 The superoxide-generating NADPH oxidase is converted to an active state by the assembly of a membrane-localized cytochrome b(559) with three cytosolic components: p47(phox), p67(phox), and GTPase Rac1 or Rac2. Superoxides 4-14 pleckstrin Homo sapiens 178-182 16391519-10 2005 Taken together, RhoA, ROCK, p38MAPK, ERK1/2, and p47(PHOX) may be subsequently activated, leading to activation of NADPH oxidase to produce superoxide. Superoxides 140-150 pleckstrin Homo sapiens 49-52 17332699-2 2007 First, the discovery of 7 Nox/Duox family proteins, Noxo1 and Noxa1 (homologues of gp91(phox), p47(phox) and p67(phox)) may clarify novel physiological mechanisms for superoxide regulation in various organs, such as the regulation of blood pressure, mucosal defense system in respiratory/digestive tract and nephron. Superoxides 167-177 pleckstrin Homo sapiens 88-92 17332699-2 2007 First, the discovery of 7 Nox/Duox family proteins, Noxo1 and Noxa1 (homologues of gp91(phox), p47(phox) and p67(phox)) may clarify novel physiological mechanisms for superoxide regulation in various organs, such as the regulation of blood pressure, mucosal defense system in respiratory/digestive tract and nephron. Superoxides 167-177 pleckstrin Homo sapiens 95-104 17332699-2 2007 First, the discovery of 7 Nox/Duox family proteins, Noxo1 and Noxa1 (homologues of gp91(phox), p47(phox) and p67(phox)) may clarify novel physiological mechanisms for superoxide regulation in various organs, such as the regulation of blood pressure, mucosal defense system in respiratory/digestive tract and nephron. Superoxides 167-177 pleckstrin Homo sapiens 99-103 16846837-0 2006 Taurine chloramine inhibits PMA-stimulated superoxide production in human neutrophils perhaps by inhibiting phosphorylation and translocation of p47(phox). Superoxides 43-53 pleckstrin Homo sapiens 145-148 16846837-0 2006 Taurine chloramine inhibits PMA-stimulated superoxide production in human neutrophils perhaps by inhibiting phosphorylation and translocation of p47(phox). Superoxides 43-53 pleckstrin Homo sapiens 149-153 16405902-9 2006 fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins p47(phox), p67(phox), and rac to the cell membrane were suppressed in parallel with the suppression of the stimulus-induced superoxide generation. Superoxides 213-223 pleckstrin Homo sapiens 89-92 16963617-7 2007 Furthermore, RNA interference of NAD(P)H oxidase subunits Nox1 or p47 markedly blocked OXO-induced increases in both extra- and intracellular O(2)(*-) levels, whereas small inhibitory RNA of Nox4 only attenuated intracellular O(2)(*-) accumulation. Superoxides 142-146 pleckstrin Homo sapiens 66-69 16115878-1 2005 The superoxide-producing phagocyte NADPH oxidase consists of a membrane-bound flavocytochrome b(558), the cytosol factors p47(phox), p67(phox), p40(phox), and the small GTPase Rac2, which translocate to the membrane to assemble the active complex following neutrophil activation. Superoxides 4-14 pleckstrin Homo sapiens 122-125 16254825-5 2005 FMLP-induced tyrosyl phosphorylation or PMA-induced serine/threonine phosphorylation and the translocation of the cytosolic proteins p47(phox) and p67(phox) to the cell membrane were suppressed in parallel to the suppression of the stimulus-induced superoxide generation. Superoxides 249-259 pleckstrin Homo sapiens 133-142 16254825-5 2005 FMLP-induced tyrosyl phosphorylation or PMA-induced serine/threonine phosphorylation and the translocation of the cytosolic proteins p47(phox) and p67(phox) to the cell membrane were suppressed in parallel to the suppression of the stimulus-induced superoxide generation. Superoxides 249-259 pleckstrin Homo sapiens 137-141 16104590-0 2005 [super-molecular interaction of p47(phox): a regulatory protein of superoxide-producing system in phagocytes]. Superoxides 67-77 pleckstrin Homo sapiens 32-35 15698597-15 2005 CONCLUSION: D,L-homocysteine and D,L-homocysteine-thiolactone enhanced fMLP-induced superoxide generation by the increment of translocation to membrane of p47(phox) and p67(phox). Superoxides 84-94 pleckstrin Homo sapiens 159-163 15698597-16 2005 L-cystathionine and N-acetyl-L-cysteine suppressed fMLP- and PMA-induced superoxide generation by the inhibition of translocation to membrane of p47(phox) and p67(phox). Superoxides 73-83 pleckstrin Homo sapiens 145-154 15698597-16 2005 L-cystathionine and N-acetyl-L-cysteine suppressed fMLP- and PMA-induced superoxide generation by the inhibition of translocation to membrane of p47(phox) and p67(phox). Superoxides 73-83 pleckstrin Homo sapiens 149-153 15326186-6 2004 NOX3-dependent superoxide production required a stimulus in the absence of subunits and upon co-expression with phagocyte NADPH oxidase subunits p47(phox) and p67(phox), but it was stimulus-independent upon co-expression with colon NADPH oxidase subunits NOX organizer 1 and NOX activator 1. Superoxides 15-25 pleckstrin Homo sapiens 145-154 15326186-6 2004 NOX3-dependent superoxide production required a stimulus in the absence of subunits and upon co-expression with phagocyte NADPH oxidase subunits p47(phox) and p67(phox), but it was stimulus-independent upon co-expression with colon NADPH oxidase subunits NOX organizer 1 and NOX activator 1. Superoxides 15-25 pleckstrin Homo sapiens 149-153 12475976-2 2003 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membrane-associated flavocytochrome b(559) and four cytosolic components as follows: p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 161-164 12650641-5 2003 Supplementing neutrophil membranes with phosphoinositides or other negatively charged phospholipids markedly enhanced cell-free superoxide generation by these p47-phox mutants in the absence of arachidonic acid, to levels equivalent to those generated by wild-type p47-phox following arachidonic acid activation. Superoxides 128-138 pleckstrin Homo sapiens 159-162 12650641-5 2003 Supplementing neutrophil membranes with phosphoinositides or other negatively charged phospholipids markedly enhanced cell-free superoxide generation by these p47-phox mutants in the absence of arachidonic acid, to levels equivalent to those generated by wild-type p47-phox following arachidonic acid activation. Superoxides 128-138 pleckstrin Homo sapiens 265-268 12663375-13 2003 CONCLUSIONS: c-Src regulates NAD(P)H oxidase-derived *O2- generation acutely by stimulating p47phox phosphorylation and translocation and chronically by increasing protein content of gp91phox, p22phox, and p47phox in Ang II-stimulated cells. Superoxides 54-56 pleckstrin Homo sapiens 92-95 12473664-3 2003 Here we show that NOX1 generates superoxide when co-expressed with the p47(phox) and p67(phox) subunits of the phagocyte NADPH oxidase but not when expressed by itself. Superoxides 33-43 pleckstrin Homo sapiens 71-80 12473664-3 2003 Here we show that NOX1 generates superoxide when co-expressed with the p47(phox) and p67(phox) subunits of the phagocyte NADPH oxidase but not when expressed by itself. Superoxides 33-43 pleckstrin Homo sapiens 75-79 14521919-6 2003 The expressions of p67 and p47(phox) were reduced by the addition of apocynin, aminoguanidine or ONO 1714 whereas xanthine oxidase and cyclooxygenase did not have a major role in superoxide production. Superoxides 179-189 pleckstrin Homo sapiens 27-30 11145703-1 2001 Generation of superoxide anion by the multiprotein complex NADPH phagocyte oxidase is accompanied by extensive phosphorylation of its 47-kDa protein component, p47(phox), a major cytosolic component of this oxidase. Superoxides 14-30 pleckstrin Homo sapiens 160-163 12456638-1 2002 Activation of the superoxide-producing phagocyte NADPH oxidase, crucial in host defense, requires the cytosolic proteins p67(phox) and p47(phox). Superoxides 18-28 pleckstrin Homo sapiens 135-138 12456638-4 2002 Here we show that p40(phox) enhances membrane translocation of p67(phox) and p47(phox) in stimulated cells, which leads to facilitated production of superoxide. Superoxides 149-159 pleckstrin Homo sapiens 77-80 11733522-1 2002 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 154-158 11733522-1 2002 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 168-172 11733522-1 2002 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 213-216 11733522-1 2002 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 168-172 11733522-1 2002 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 168-172 11733522-1 2002 The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). Superoxides 4-14 pleckstrin Homo sapiens 168-172 12056906-1 2002 Production of superoxide anions by the multicomponent enzyme of human neutrophil NADPH oxidase is accompanied by extensive phosphorylation of p47(phox), one of its cytosolic components. Superoxides 14-31 pleckstrin Homo sapiens 142-145 12225397-9 2002 We speculate that the remarkable induction of gp91phox and p47phox protein is associated with an increase in superoxide-generating activity due to the synergistic effect of ATRA plus GM-CSF. Superoxides 109-119 pleckstrin Homo sapiens 59-62 11896062-1 2002 Activation of the superoxide-generating NADPH oxidase of phagocytes is the result of the assembly of a membrane-localized flavocytochrome (cytochrome b(559)) with the cytosolic components p47(phox), p67(phox), and the small GTPase Rac. Superoxides 18-28 pleckstrin Homo sapiens 188-191 11813269-7 2002 The p91 and p47 components of the NADPH oxidase increase after IFN-gamma stimulation and may account for the enhanced superoxide generation. Superoxides 118-128 pleckstrin Homo sapiens 12-15 11813269-8 2002 Antisense experiments targeting p91 and p47 subunits abrogate the increased osteoclastic superoxide production stimulated by IFN-gamma. Superoxides 89-99 pleckstrin Homo sapiens 40-43 11726431-1 2001 UNLABELLED: Superoxide is generated by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase that exists in the cell membrane of neutrophils and contains p47 phox in the enzyme complex. Superoxides 12-22 pleckstrin Homo sapiens 168-171 11726431-9 2001 Our results suggest that suppression of superoxide production by a therapeutic dose of lidocaine correlates strongly with suppression of p47 phox translocation. Superoxides 40-50 pleckstrin Homo sapiens 137-140 11705402-1 2001 Activation of the phagocyte NADPH oxidase, a superoxide-generating enzyme, involves assembly of cytosolic p47(phox), p67(phox), and rac with the membrane-associated cytochrome b(558). Superoxides 45-55 pleckstrin Homo sapiens 106-109 11397907-6 2001 The level of p47(phox) subunit, an index of NADPH oxidase, the enzyme converting molecular oxygen to superoxide (O(. Superoxides 101-111 pleckstrin Homo sapiens 13-16 11348868-6 2001 Furthermore, the injury-induced superoxide production was associated with augmented NAD(P)H oxidase activity and upregulation of p47(phox) and p67(phox) in adventitial fibroblasts (immunohistochemistry). Superoxides 32-42 pleckstrin Homo sapiens 129-138 11348868-6 2001 Furthermore, the injury-induced superoxide production was associated with augmented NAD(P)H oxidase activity and upregulation of p47(phox) and p67(phox) in adventitial fibroblasts (immunohistochemistry). Superoxides 32-42 pleckstrin Homo sapiens 133-137 10788501-1 2000 The superoxide-producing phagocyte NADPH oxidase can be activated by arachidonic acid (AA) or by phosphorylation of p47(phox) under cell-free conditions. Superoxides 4-14 pleckstrin Homo sapiens 116-119 10788501-1 2000 The superoxide-producing phagocyte NADPH oxidase can be activated by arachidonic acid (AA) or by phosphorylation of p47(phox) under cell-free conditions. Superoxides 4-14 pleckstrin Homo sapiens 120-124 10788501-7 2000 Furthermore, phosphorylated p47(phox) effectively binds to p22(phox) and activates the oxidase in the presence of AA at low concentrations (1-5 micrometer), where an unphosphorylated protein only slightly supports superoxide production. Superoxides 214-224 pleckstrin Homo sapiens 28-31 10788501-7 2000 Furthermore, phosphorylated p47(phox) effectively binds to p22(phox) and activates the oxidase in the presence of AA at low concentrations (1-5 micrometer), where an unphosphorylated protein only slightly supports superoxide production. Superoxides 214-224 pleckstrin Homo sapiens 32-36 10559253-2 1999 Activation of the superoxide-producing phagocyte NADPH oxidase requires interaction between p47(phox) and p22(phox), which is mediated via the SH3 domains of the former protein. Superoxides 18-28 pleckstrin Homo sapiens 92-101 10559253-2 1999 Activation of the superoxide-producing phagocyte NADPH oxidase requires interaction between p47(phox) and p22(phox), which is mediated via the SH3 domains of the former protein. Superoxides 18-28 pleckstrin Homo sapiens 96-100 10400704-5 1999 GM-CSF-induced p47(phox) phosphorylation was time- and concentration-dependent and ran parallel to the priming effect of GM-CSF on superoxide production. Superoxides 131-141 pleckstrin Homo sapiens 15-18 10400704-5 1999 GM-CSF-induced p47(phox) phosphorylation was time- and concentration-dependent and ran parallel to the priming effect of GM-CSF on superoxide production. Superoxides 131-141 pleckstrin Homo sapiens 19-23