PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
16118223-7	2005	The H372R substitution results in an increase in polymerization-dependent fluorescence of Cys-374 pyrene-labeled actin.	pyrene	98-104	actin	Saccharomyces cerevisiae S288C	113-118	
16326906-2	2006	Saturating cardiac tropomyosin (cTM) caused about a 20% increase in pyrene fluorescence of the doubly labeled F-actin but no change in WT actin C374 probe fluorescence.	pyrene	68-74	actin	Saccharomyces cerevisiae S288C	112-117	
27526190-3	2016	Using live cell imaging, in vivo and in vitro protein binding assays, and in vitro pyrene-actin polymerization assays, we show that the yeast Rab GTPase Sec4p couples polarized exocytosis with cortical actin polymerization, which induces endocytosis.	pyrene	83-89	actin	Saccharomyces cerevisiae S288C	90-95	
12414705-6	2002	A Ca(2+)-independent conformational change in that region was detected upon troponin binding to actin-Tm via an increase in the fluorescence of a pyrene probe attached to another yeast actin mutant that we used (Cys(1)).	pyrene	146-152	actin	Saccharomyces cerevisiae S288C	96-101	
12414705-6	2002	A Ca(2+)-independent conformational change in that region was detected upon troponin binding to actin-Tm via an increase in the fluorescence of a pyrene probe attached to another yeast actin mutant that we used (Cys(1)).	pyrene	146-152	actin	Saccharomyces cerevisiae S288C	185-190	
8552682-1	1996	Wild-type actin and a mutant actin were isolated from yeast (Saccharomyces cerevisiae) and the polymerization properties were examined at pH 8.0 and 20 degrees C. The polymerization reaction was followed either by an increase in pyrene-labeled actin fluorescence or by a decrease in intrinsic fluorescence in the absence of pyrene-labeled actin.	pyrene	229-235	actin	Saccharomyces cerevisiae S288C	29-34	
8552682-1	1996	Wild-type actin and a mutant actin were isolated from yeast (Saccharomyces cerevisiae) and the polymerization properties were examined at pH 8.0 and 20 degrees C. The polymerization reaction was followed either by an increase in pyrene-labeled actin fluorescence or by a decrease in intrinsic fluorescence in the absence of pyrene-labeled actin.	pyrene	229-235	actin	Saccharomyces cerevisiae S288C	29-34	
8552682-1	1996	Wild-type actin and a mutant actin were isolated from yeast (Saccharomyces cerevisiae) and the polymerization properties were examined at pH 8.0 and 20 degrees C. The polymerization reaction was followed either by an increase in pyrene-labeled actin fluorescence or by a decrease in intrinsic fluorescence in the absence of pyrene-labeled actin.	pyrene	229-235	actin	Saccharomyces cerevisiae S288C	29-34	
8552682-5	1996	Second, as measured with pyrene-labeled yeast actin, but not with intrinsic fluorescence, there is an overshoot in the fluorescence that has not been observed with skeletal muscle actin under the same conditions.	pyrene	25-31	actin	Saccharomyces cerevisiae S288C	46-51	
11959868-3	2002	Pyrene at each of these positions produced differing fluorescence spectra in G-actin.	pyrene	0-6	actin	Saccharomyces cerevisiae S288C	79-84	
11959868-8	2002	F-actin quenching was biphasic for the 265, 266, and 269 but not 267 probes, suggesting that in F-actin, the pyrene samples multiple environments.	pyrene	109-115	actin	Saccharomyces cerevisiae S288C	2-7	
11959868-8	2002	F-actin quenching was biphasic for the 265, 266, and 269 but not 267 probes, suggesting that in F-actin, the pyrene samples multiple environments.	pyrene	109-115	actin	Saccharomyces cerevisiae S288C	98-103	
11959868-9	2002	Finally, in F-actin the probe at 266 interacts with one at Cys(374) on a monomer in the opposing strand, producing a pyrene excimer band.	pyrene	117-123	actin	Saccharomyces cerevisiae S288C	14-19	
10677229-9	2000	This demonstrates a transition at the S1 contact with actin"s N-terminus between the weakly and strongly bound states, and implies either a closer proximity of the pyrene probe on Cys-1 to structural elements on S1 (most likely the loop of residues 626-647) or greater S1-induced changes at the N-terminus of actin in the weakly bound acto-S1 states.	pyrene	164-170	actin	Saccharomyces cerevisiae S288C	54-59