PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
20060364-4	2010	The values of the rate constants are estimated by integrating these ODEs numerically and fitting them to a series of stopped-flow pyrene fluorescence transients of myosin-S1 fragment binding to regulated actin in solution.	pyrene	130-136	myosin heavy chain 14	Homo sapiens	164-170	
25095992-0	2014	Use of pyrene-labelled actin to probe actin-myosin interactions: kinetic and equilibrium studies.	pyrene	7-13	myosin heavy chain 14	Homo sapiens	44-50	
25095992-2	2014	The fluorescent label pyrene, covalently attached to actin (at Cys 374), has been one of the most useful optical probes to report myosin binding to actin.	pyrene	22-28	myosin heavy chain 14	Homo sapiens	130-136	
3911945-1	1985	A pyrene label attached to Cys-374 of actin has been shown to be a useful probe for monitoring the interaction of actin with myosin subfragments [Kouyama & Mihashi (1981) Eur.	pyrene	2-8	myosin heavy chain 14	Homo sapiens	125-131	
140201-2	1976	Addition of ATP to PM-myosin produces a reversible decrease of 10% in fluorescence intensity of the pyrene fluorophore in the presence of actin.	pyrene	100-106	myosin heavy chain 14	Homo sapiens	22-28	
14596602-6	2003	We then assessed the ability of these and wild-type myosin to bind strongly via two heads to an actin filament by measuring the fluorescence quenching of pyrene-labeled actin induced by myosin binding.	pyrene	154-160	myosin heavy chain 14	Homo sapiens	52-58	
15065866-3	2004	Utilizing the fluorescence signal on binding of myosin to pyrene-labeled actin filaments, we investigated the interplay of actin and nucleotide binding to thiophosphorylated and unphosphorylated recombinant nonmuscle IIA heavy meromyosin constructs.	pyrene	58-64	myosin heavy chain 14	Homo sapiens	48-54	
14596602-6	2003	We then assessed the ability of these and wild-type myosin to bind strongly via two heads to an actin filament by measuring the fluorescence quenching of pyrene-labeled actin induced by myosin binding.	pyrene	154-160	myosin heavy chain 14	Homo sapiens	186-192	
10730576-1	1999	The dynamics of the interaction of fast skeletal muscle myosin subfragment-1 with pyrene-labelled actin were examined using both stopped-flow and pressure relaxation methods.	pyrene	82-88	myosin heavy chain 14	Homo sapiens	56-62	
1953663-2	1991	The co-operative binding of myosin subfragment 1 (S1) to reconstituted skeletal-muscle thin filaments has been examined by monitoring the fluorescence of a pyrene probe on Cys-374 of actin.	pyrene	156-162	myosin heavy chain 14	Homo sapiens	28-34	
1845966-1	1991	The reactions of pyrene-labeled actin with myosin subfragment 1 (S1) and S1-ligand complexes at low ionic strength are described by the schemes [formula: see text] where M refers to a myosin head; A is actin; L is ligand; the asterisk refers to a high fluorescence state of actin; and K1 and K3 are association constants.	pyrene	17-23	myosin heavy chain 14	Homo sapiens	43-49	
1845966-1	1991	The reactions of pyrene-labeled actin with myosin subfragment 1 (S1) and S1-ligand complexes at low ionic strength are described by the schemes [formula: see text] where M refers to a myosin head; A is actin; L is ligand; the asterisk refers to a high fluorescence state of actin; and K1 and K3 are association constants.	pyrene	17-23	myosin heavy chain 14	Homo sapiens	184-190