PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30472464-8 2019 Further, the smoking cessation agents, nicotine, varenicline and cytisine increased activation of mutant (alpha4)3(beta2)2 receptors, while only nicotine increased activation of mutant (alpha4)2(beta2)3 receptors. Nicotine 39-47 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 106-120 30472464-8 2019 Further, the smoking cessation agents, nicotine, varenicline and cytisine increased activation of mutant (alpha4)3(beta2)2 receptors, while only nicotine increased activation of mutant (alpha4)2(beta2)3 receptors. Nicotine 145-153 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 186-200 30326594-1 2018 Activation of nicotinic acetylcholine receptors containing alpha4 and beta2 subunits (alpha4/beta2* nAChRs) in the mammalian brain is necessary for nicotine reinforcement and addiction. Nicotine 148-156 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 93-98 29720657-9 2018 Antibody fragments specific to beta2 were used to "break" symmetry during particle alignment and to obtain high-resolution reconstructions of receptors of both stoichiometries in complex with nicotine. Nicotine 192-200 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 31-36 29110618-6 2018 Recent studies have demonstrated that the alpha4, beta2, and alpha7 subunits of the nicotinic acetylcholine receptor (nAChR) participate in the cognitive-enhancing effects of nicotine. Nicotine 175-183 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 50-55 28803192-5 2018 After adjustment for confounders, prenatal nicotine concentration levels were negatively associated with communication (beta=-2.059; p=0.015) and fine motor skills (beta=-2.120; p=0.002) while postnatal nicotine concentration levels were inversely associated with fine motors (beta=-0.124; p=0.004) and problem solving skills (beta=-0.117; p=0.013). Nicotine 43-51 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 120-127 28803192-5 2018 After adjustment for confounders, prenatal nicotine concentration levels were negatively associated with communication (beta=-2.059; p=0.015) and fine motor skills (beta=-2.120; p=0.002) while postnatal nicotine concentration levels were inversely associated with fine motors (beta=-0.124; p=0.004) and problem solving skills (beta=-0.117; p=0.013). Nicotine 43-51 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 165-172 28235547-9 2017 These findings indicate that the nAChRs (predominantly with the alpha2/beta2 complex) are affected by IHH in critical hippocampal and brainstem nuclei during early brain development, and that pre-exposure to nicotine alters the pattern of susceptibility to IHH. Nicotine 208-216 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 71-76 27559543-4 2016 We observed significant effects of nicotine exposure on the beta2*-nAChR-associated proteome in human and mouse cortex, particularly in the abundance of the nAChR subunits themselves, as well as putative interacting proteins that make up core components of neuronal excitability (Na/K ATPase subunits), presynaptic neurotransmitter release (syntaxins, SNAP25, synaptotagmin), and a member of a known nAChR protein chaperone family (14-3-3zeta). Nicotine 35-43 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 60-65 26619345-1 2015 High affinity nicotine-binding sites in the mammalian brain are neuronal nicotinic acetylcholine receptors (nAChR) assembled from at least alpha4 and beta2 subunits into pentameric ion channels. Nicotine 14-22 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 150-155 26269589-7 2015 We also used single molecule fluorescence studies to show that cotinine and nicotine both alter the assembly of alpha4beta2 receptors to favor the high sensitivity (alpha4)2(beta2)3 stoichiometry. Nicotine 76-84 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 118-123 24051136-0 2013 Selective potentiation of (alpha4)3(beta2)2 nicotinic acetylcholine receptors augments amplitudes of prefrontal acetylcholine- and nicotine-evoked glutamatergic transients in rats. Nicotine 131-139 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 27-41 23891776-1 2013 Neuronal nicotinic acetylcholine receptors (nAChRs) containing alpha4 and beta2 subunits are the principal receptors in the mammalian central nervous system that bind nicotine with high affinity. Nicotine 167-175 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 74-79 23586521-12 2013 Acute nicotine (30 min exposure) was sufficient to upregulate FRET between alpha4 and beta2 subunits. Nicotine 6-14 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 86-91 22349182-8 2012 RT-PCR analysis revealed that nicotine exposure resulted in the upregulation of alpha3 and beta4 and the downregulation of alpha4-nAChR, while alpha7- and beta2-nAChR-mRNA expressions remained unaltered. Nicotine 30-38 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 155-160 22949846-7 2012 The simulations also account for previous macroscopic and single-channel observations that pharmacological chaperoning by nicotine and cytisine increase the (alpha4)(2)(beta2)(3) and (alpha4)(3)(beta2)(2) populations, respectively. Nicotine 122-130 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 169-174 22949846-7 2012 The simulations also account for previous macroscopic and single-channel observations that pharmacological chaperoning by nicotine and cytisine increase the (alpha4)(2)(beta2)(3) and (alpha4)(3)(beta2)(2) populations, respectively. Nicotine 122-130 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 195-200 21940627-4 2011 Nicotine markedly increased alpha4beta2 nAChR binding site density and beta2 subunit protein. Nicotine 0-8 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 34-39 21252231-2 2011 The predominant nAChR subtype in the mammalian brain with a high affinity for nicotine is composed of alpha4 and beta2 subunits. Nicotine 78-86 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 113-118 21187334-5 2011 Pixel-resolved normalized Forster resonance energy transfer microscopy between alpha4-FP subunits shows that nicotine stabilizes the (alpha4)(2)(beta2)(3) stoichiometry before the nAChRs reach the trans-Golgi apparatus. Nicotine 109-117 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 145-150 21187334-10 2011 The experimental data are simulated with a model incorporating two mechanisms: (1) nicotine acts as a stabilizing pharmacological chaperone for nascent alpha4beta2 nAChRs in the ER, eventually increasing PM receptors despite a bottleneck(s) in ER export; and (2) removal of the bottleneck (e.g., by expression of the beta2(enhanced-ER-export) subunit) is sufficient to increase PM nAChR numbers, even without nicotine. Nicotine 83-91 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 158-163 20837109-2 2010 Using a pharmacological approach to investigate the effects of nicotine on receptor subunit expression and phosphorylation in SH-EP1 cells expressing human alpha4 and beta2 nicotinic receptor subunits, we have demonstrated that incubation with nicotine for 24 h increased the expression of immature and mature forms of both alpha4 and beta2 subunits in a concentration-dependent manner, and that inhibition of protein kinase C (PKC), but not cAMP-dependent protein kinase (PKA) inhibited the nicotine-induced increased expression of subunits. Nicotine 244-252 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 167-172 20837109-2 2010 Using a pharmacological approach to investigate the effects of nicotine on receptor subunit expression and phosphorylation in SH-EP1 cells expressing human alpha4 and beta2 nicotinic receptor subunits, we have demonstrated that incubation with nicotine for 24 h increased the expression of immature and mature forms of both alpha4 and beta2 subunits in a concentration-dependent manner, and that inhibition of protein kinase C (PKC), but not cAMP-dependent protein kinase (PKA) inhibited the nicotine-induced increased expression of subunits. Nicotine 244-252 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 335-340 20598018-2 2010 This frequency-dependent inhibition is because of nicotine desensitizing heteromeric beta2 subunit-containing nicotinic acetylcholine receptor (nAChR) subtypes. Nicotine 50-58 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 85-90 20598018-3 2010 Surprisingly, a high dose of nicotine (2 muM; capable of interacting with additional nAChR subtypes) produced an inhibition of dopamine evoked by high frequency stimulation, an effect that was not seen with the low dose of nicotine or the beta2 antagonist, dihydro-beta-erythroidine hydrobromide. Nicotine 29-37 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 239-244 20566638-1 2010 In the mammalian brain high affinity nicotine-binding sites are composed of at least the alpha4 and beta2 subunits. Nicotine 37-45 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 100-105 20566638-3 2010 The introduction of alpha5 into 293 cells expressing alpha4+beta2 strongly favors assembly of alpha4+alpha5+beta2 receptors, increases constitutive ligand binding density as measured using [(3)H]epibatidine, but reduces the magnitude of up-regulation in response to chronic nicotine. Nicotine 274-282 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 60-65 20392695-1 2010 Neuronal nicotinic acetylcholine receptors (nAChR) composed of alpha4 + beta2 subunits, the high affinity nicotine-binding site in the mammalian brain, up-regulate in response to chronic nicotine exposure. Nicotine 106-114 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 72-77 20392695-1 2010 Neuronal nicotinic acetylcholine receptors (nAChR) composed of alpha4 + beta2 subunits, the high affinity nicotine-binding site in the mammalian brain, up-regulate in response to chronic nicotine exposure. Nicotine 187-195 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 72-77 20400893-2 2010 Although numerous studies have shown that high-affinity beta2-containing nAChRs are necessary for the nicotine-induced enhancement of contextual fear conditioning, it is unknown whether other high-affinity nAChR agonists are capable of enhancing this learning. Nicotine 102-110 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 56-61 19896527-2 2010 We tested the hypothesis that nicotine increases expression of the nicotinic acetylcholine receptor (nAChR) subunits alpha7 and beta2 in a piglet model. Nicotine 30-38 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 128-133 19896527-5 2010 Compared to controls, nicotine exposed piglets had decreased alpha7 in the rostral dorsal motor nucleus of the vagus (rDMNV) (p=0.01), and increased beta2 in the caudal DMNV (cDMNV) (p=0.05), caudal nucleus of the spinal trigeminal tract (cNSTT) (p=0.03) and caudal nucleus of the solitary tract (cNTS) (p=0.04). Nicotine 22-30 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 149-154 19896527-7 2010 Compared to control males, nicotine exposed males had lower beta2 in the cXII (p<0.01). Nicotine 27-35 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 60-65 19778551-13 2009 These results indicate that beta2* and potentially alpha3beta4* nicotinic acetylcholine receptors play a role in the CS effects of nicotine and are potential targets for the development of nicotine cessation aids. Nicotine 131-139 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 28-33 19778551-13 2009 These results indicate that beta2* and potentially alpha3beta4* nicotinic acetylcholine receptors play a role in the CS effects of nicotine and are potential targets for the development of nicotine cessation aids. Nicotine 189-197 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 28-33 19482438-0 2009 Association of genes coding for the alpha-4, alpha-5, beta-2 and beta-3 subunits of nicotinic receptors with cigarette smoking and nicotine dependence. Nicotine 131-139 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 54-60 19237585-8 2009 In contrast, 24- to 48-h nicotine (1 muM) exposure increased the proportion of (alpha4)(2)(beta2)(3) in WT receptors and also returned subunit stoichiometry to WT levels for alpha4S248F and beta2V287L nAChRs. Nicotine 25-33 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 91-96 18991851-3 2008 A majority of high-affinity nicotine binding sites in the brain have been showed in heteropentameric alpha4 (alpha4) and beta2 subunit (beta2) of nAChRs. Nicotine 28-36 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 121-126 18762859-2 2008 The majority of high affinity nicotine binding sites in the human brain have been implicated in heteropentameric alpha4 and beta2 subunits of neuronal nicotinic acetylcholine receptors; therefore, these two neuronal nicotinic acetylcholine receptors genes (CHRNA4 and CHRNB2) are considered to be attractive candidate genes for the pathophysiology of schizophrenia. Nicotine 30-38 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 124-129 18596163-2 2008 The alpha4 and beta2 nAChR subunits assemble into two alternate stoichiometries, (alpha4)(2)(beta2)(3) and (alpha4)(3)(beta2)(2), which differ in their functional properties and sensitivity to chronic exposure to nicotine. Nicotine 213-221 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 15-20 17932221-2 2008 Nicotinic receptors containing alpha4, alpha6, beta2, and beta3 subunits are expressed in midbrain dopaminergic neurons, and they are implicated in the response to smoked nicotine. Nicotine 171-179 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 47-63 17470777-4 2007 These data offer previously undescribed insights into the understanding of nicotine addiction and the treatment of several human pathologies by nicotine-like agents chronically acting on beta2*- or alpha7*nAChRs. Nicotine 75-83 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 187-192 17470777-4 2007 These data offer previously undescribed insights into the understanding of nicotine addiction and the treatment of several human pathologies by nicotine-like agents chronically acting on beta2*- or alpha7*nAChRs. Nicotine 144-152 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 187-192 16928859-2 2006 In particular, nAChRs containing beta2-subunits (beta2*-nAChRs) the most prevalent subtype, mediate the reinforcing properties of nicotine. Nicotine 130-138 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 33-38 16928859-2 2006 In particular, nAChRs containing beta2-subunits (beta2*-nAChRs) the most prevalent subtype, mediate the reinforcing properties of nicotine. Nicotine 130-138 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 49-54 16928859-9 2006 Higher brain beta2*-nAChR during early abstinence indicates that, when smokers quit smoking, they do so in the face of a significant increase in the receptors normally activated by nicotine. Nicotine 181-189 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 13-18 16485261-4 2006 In this study, we have examined the effects of chronic nicotine treatment on alpha7 and beta2 nAChR subunits in vitro in cell lines and in vivo in mouse striatum. Nicotine 55-63 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 88-93 17192649-7 2006 Intra-VTA injection of nicotine was found to be reinforcing in both wild-type and beta2-subunit re-expressing beta2-/- mice, but not in beta2-/- mice. Nicotine 23-31 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 82-87 17192649-7 2006 Intra-VTA injection of nicotine was found to be reinforcing in both wild-type and beta2-subunit re-expressing beta2-/- mice, but not in beta2-/- mice. Nicotine 23-31 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 110-115 15944384-1 2005 Nicotine addiction is initiated by its binding to high-affinity nicotinic receptors in brain composed primarily of alpha4 and beta2 subunits. Nicotine 0-8 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 126-131 15569257-7 2004 Similar IC50 values have been reported for the MLA inhibition of nicotine-stimulated dopamine release, a response that is mediated by beta2-subunit-containing nAchRs and not alpha7-subunit-containing nAchRs. Nicotine 65-73 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 134-139 14764595-6 2004 (ii) Only residues belonging to two beta2 segments, 74-89 and 106-115, confer up-regulation to beta4, mainly by decreasing the amount of binding sites in the absence of nicotine; on an atomic three-dimensional model of the alpha3beta2 receptor these amino acids form a compact microdomain that mainly contributes to the subunit interface and also faces the acetylcholine binding site. Nicotine 169-177 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 36-41 14684858-4 2003 Transfected midbrain neurons that were exposed to nicotine (1 d) displayed greater levels of fluorescent alpha4 and beta2 nicotinic ACh receptor (nAChR) subunits. Nicotine 50-58 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 116-121 14645658-1 2003 Naturally expressed nicotinic acetylcholine receptors composed of alpha4 and beta2 subunits (alpha4beta2-nAChR) are the predominant form of high affinity nicotine binding site in the brain implicated in nicotine reward, mediation of nicotinic cholinergic transmission, modulation of signaling through other chemical messages, and a number of neuropsychiatric disorders. Nicotine 154-162 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 77-82 14645658-1 2003 Naturally expressed nicotinic acetylcholine receptors composed of alpha4 and beta2 subunits (alpha4beta2-nAChR) are the predominant form of high affinity nicotine binding site in the brain implicated in nicotine reward, mediation of nicotinic cholinergic transmission, modulation of signaling through other chemical messages, and a number of neuropsychiatric disorders. Nicotine 203-211 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 77-82 12527804-5 2003 Overnight treatment with nicotine increased the number of nAChRs and increased the proportion of the (alpha4)(2)(beta2)(3) stoichiometry. Nicotine 25-33 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 113-118 12480162-1 2003 Cytisine and nicotine bound to specific sites in homogenates prepared from HEK 293 cells which stably express human neuronal nicotinic alpha4 and beta2 subunits. Nicotine 13-21 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 146-151 12021403-0 2002 First and second transmembrane segments of alpha3, alpha4, beta2, and beta4 nicotinic acetylcholine receptor subunits influence the efficacy and potency of nicotine. Nicotine 156-164 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 59-64 11859855-6 2002 Evidence also has been presented to indicate that nicotine acts on alpha7-nicotinic receptors located on sympathetic nerve terminals, resulting in release of norepinephrine which then diffuses to act on beta2-adrenoceptos located on the neighboring nitrergic nerve terminals to release NO and therefore vasodilation. Nicotine 50-58 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 203-208 10854263-10 2000 Co-expression of the human alpha5 subunit with alpha3 and beta2 subunits had the effect of producing protracted responses to ACh and increasing residual inhibition by ACh and nicotine but not RJR-2403. Nicotine 175-183 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 58-63 10441742-1 1999 As a first step in determining whether there are polymorphisms in the nicotinic acetylcholine receptor (nAChR) genes that are associated with nicotine addiction, we isolated genomic clones of the beta2-nAChR genes from human and mouse BAC libraries. Nicotine 142-150 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 196-201 9786868-0 1998 Chronic nicotine treatment up-regulates human alpha3 beta2 but not alpha3 beta4 acetylcholine receptors stably transfected in human embryonic kidney cells. Nicotine 8-16 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 53-58 9786868-3 1998 Nicotine was a partial agonist on alpha3 beta4 AChRs and nearly a full agonist on alpha3 beta2 alpha5 AChRs. Nicotine 0-8 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 89-94 9786868-4 1998 Chronic exposure of cells expressing alpha3 beta2 AChRs or alpha3 beta2 alpha5 AChRs to nicotine or carbamylcholine increased their amount up to 24-fold but had no effect on the amount of alpha3beta4 or alpha3 beta4 alpha5 AChRs, i.e. the up-regulation of alpha3 AChRs depended on the presence of beta2 but not beta4 subunits in the AChRs. Nicotine 88-96 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 44-49 9786868-4 1998 Chronic exposure of cells expressing alpha3 beta2 AChRs or alpha3 beta2 alpha5 AChRs to nicotine or carbamylcholine increased their amount up to 24-fold but had no effect on the amount of alpha3beta4 or alpha3 beta4 alpha5 AChRs, i.e. the up-regulation of alpha3 AChRs depended on the presence of beta2 but not beta4 subunits in the AChRs. Nicotine 88-96 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 66-71 9786868-4 1998 Chronic exposure of cells expressing alpha3 beta2 AChRs or alpha3 beta2 alpha5 AChRs to nicotine or carbamylcholine increased their amount up to 24-fold but had no effect on the amount of alpha3beta4 or alpha3 beta4 alpha5 AChRs, i.e. the up-regulation of alpha3 AChRs depended on the presence of beta2 but not beta4 subunits in the AChRs. Nicotine 88-96 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 66-71 9786868-6 1998 In the absence of nicotine, alpha3 beta2 AChRs were expressed at much lower levels than alpha3 beta4 AChRs, but in the presence of nicotine, the amount of alpha3 beta2 AChRs exceeded that of alpha3 beta4 AChRs. Nicotine 131-139 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 162-167 9786868-11 1998 Our data suggest that up-regulation of alpha3 beta2 AChRs in these lines by nicotine results from both increased subunit assembly and decreased AChR turnover. Nicotine 76-84 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 46-51 9388554-0 1997 Contribution of nicotinic acetylcholine receptors containing the beta 2-subunit to the behavioural effects of nicotine. Nicotine 110-118 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 65-71 8663494-9 1996 However, in the case of receptors containing alpha3 and beta2 subunits, the addition of alpha5 subunits reduced the EC50 for acetylcholine from 28 to 0.5 microM and the EC50 for nicotine from 6.8 to 1.9 microM, while increasing the efficacy of nicotine from 50% on alpha3beta2 receptors to 100% on alpha3beta2alpha5 receptors. Nicotine 178-186 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 56-61 8663494-9 1996 However, in the case of receptors containing alpha3 and beta2 subunits, the addition of alpha5 subunits reduced the EC50 for acetylcholine from 28 to 0.5 microM and the EC50 for nicotine from 6.8 to 1.9 microM, while increasing the efficacy of nicotine from 50% on alpha3beta2 receptors to 100% on alpha3beta2alpha5 receptors. Nicotine 244-252 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 56-61 8558445-1 1996 (-)-Nicotine, the prototypical agonist for neuronal nicotinic acetylcholine receptors (nAChR) has been shown to bind with high affinity to the rodent and avian alpha 4 beta 2 nAChR subtype. Nicotine 0-12 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 168-174