PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 30206032-12 2019 Western blot analysis showed an increased expression of ERK1/2 in nicotine treated cultures suggesting nicotine provided neuroprotection in SCG neurons by increasing the expression of ERK1/2 through nicotinic receptor dependent mechanisms. Nicotine 66-74 mitogen activated protein kinase 3 Rattus norvegicus 56-62 30124787-5 2019 We report that exposure to nicotine, selectively during adolescence, induces profound and long-lasting neuronal, molecular and behavioral disturbances involving PFC DA D1R and downstream extracellular-signal-related kinase 1-2 (ERK 1-2) signaling. Nicotine 27-35 mitogen activated protein kinase 3 Rattus norvegicus 228-235 30206032-12 2019 Western blot analysis showed an increased expression of ERK1/2 in nicotine treated cultures suggesting nicotine provided neuroprotection in SCG neurons by increasing the expression of ERK1/2 through nicotinic receptor dependent mechanisms. Nicotine 66-74 mitogen activated protein kinase 3 Rattus norvegicus 184-190 30206032-12 2019 Western blot analysis showed an increased expression of ERK1/2 in nicotine treated cultures suggesting nicotine provided neuroprotection in SCG neurons by increasing the expression of ERK1/2 through nicotinic receptor dependent mechanisms. Nicotine 103-111 mitogen activated protein kinase 3 Rattus norvegicus 56-62 30206032-12 2019 Western blot analysis showed an increased expression of ERK1/2 in nicotine treated cultures suggesting nicotine provided neuroprotection in SCG neurons by increasing the expression of ERK1/2 through nicotinic receptor dependent mechanisms. Nicotine 103-111 mitogen activated protein kinase 3 Rattus norvegicus 184-190 25328101-0 2015 Effects of environmental enrichment on ERK1/2 phosphorylation in the rat prefrontal cortex following nicotine-induced sensitization or nicotine self-administration. Nicotine 101-109 mitogen activated protein kinase 3 Rattus norvegicus 39-45 29474782-14 2017 Exposure of cardiac cells to nicotine induced the expression of ERS markers and p38; the ERK 1/2 was highly expressed only in the presence of EGCG. Nicotine 29-37 mitogen activated protein kinase 3 Rattus norvegicus 89-96 26232787-2 2015 We have demonstrated that rats raised in an enriched condition are more sensitive than rats raised in an impoverished condition to nicotine-induced locomotor activity, and this is associated with alterations of phosphorylated extracellular signal-regulated kinase 1/2 within the prefrontal cortex. Nicotine 131-139 mitogen activated protein kinase 3 Rattus norvegicus 226-267 26232787-6 2015 Lentiviral-mediated overexpression of microRNA-221 in PC12 cells and the medial prefrontal cortex was performed to determine the effects of microRNA-221 on nicotine-mediated phosphorylated extracellular signal-regulated kinase 1/2, phosphorylated cAMP-response element-binding protein, and locomotor activity. Nicotine 156-164 mitogen activated protein kinase 3 Rattus norvegicus 189-284 26232787-8 2015 Overexpression of lentiviral-microRNA-221 attenuated nicotine-induced increase in phosphorylated extracellular signal-regulated kinase 1/2 in PC12 cells. Nicotine 53-61 mitogen activated protein kinase 3 Rattus norvegicus 97-138 26232787-10 2015 Accordingly, lentiviral-microRNA-221 attenuated nicotine-induced increases in phosphorylated extracellular signal-regulated kinase 1/2 and phosphorylated cAMP-response element-binding protein in the medial prefrontal cortex of impoverished condition but not enriched condition rats. Nicotine 48-56 mitogen activated protein kinase 3 Rattus norvegicus 93-191 25613062-9 2015 In primary cultured rat chondrocytes, pretreatment with nicotine suppressed both p38, extracellular regulated kinase (Erk) 1/2 and c-Jun-N-terminal kinase (JNK) mitogen-activated protein kinases (MAPK) phosphorylation and phosphorylated nuclear factor-kappa B (NF-kappaB) p65 activation induced by MIA- or IL-1beta, and these effects were also reversed by MLA. Nicotine 56-64 mitogen activated protein kinase 3 Rattus norvegicus 86-126 25613062-9 2015 In primary cultured rat chondrocytes, pretreatment with nicotine suppressed both p38, extracellular regulated kinase (Erk) 1/2 and c-Jun-N-terminal kinase (JNK) mitogen-activated protein kinases (MAPK) phosphorylation and phosphorylated nuclear factor-kappa B (NF-kappaB) p65 activation induced by MIA- or IL-1beta, and these effects were also reversed by MLA. Nicotine 56-64 mitogen activated protein kinase 3 Rattus norvegicus 196-200 25328101-0 2015 Effects of environmental enrichment on ERK1/2 phosphorylation in the rat prefrontal cortex following nicotine-induced sensitization or nicotine self-administration. Nicotine 135-143 mitogen activated protein kinase 3 Rattus norvegicus 39-45 25328101-2 2015 The current study determined whether environmental enrichment differentially regulates extracellular signal-regulated kinase1/2 (ERK1/2) activity in the prefrontal cortex in rats following nicotine sensitization or nicotine self-administration. Nicotine 189-197 mitogen activated protein kinase 3 Rattus norvegicus 129-135 25328101-2 2015 The current study determined whether environmental enrichment differentially regulates extracellular signal-regulated kinase1/2 (ERK1/2) activity in the prefrontal cortex in rats following nicotine sensitization or nicotine self-administration. Nicotine 215-223 mitogen activated protein kinase 3 Rattus norvegicus 129-135 23624270-2 2013 Treatment with nicotine stimulated ERK1/2 and p38 MAPK phosphorylation in the PC12 cells expressing nNOS (NPC12 cells) as compared with that in control PC12 cells. Nicotine 15-23 mitogen activated protein kinase 3 Rattus norvegicus 35-41 24885237-5 2014 After 3-4 days of IH, extracellular signal-regulated kinases 1/2 (ERK1/2) protein phosphorylation decreased, which could be reversed by superoxide dismutase (SOD), 1,10-phenanthroline (Phe), the PP2A phosphorylation inhibitors, okadaic acid (OKA) and cantharidin, and the ERK phosphorylation activator nicotine (p < 0.05). Nicotine 302-310 mitogen activated protein kinase 3 Rattus norvegicus 22-64 24885237-5 2014 After 3-4 days of IH, extracellular signal-regulated kinases 1/2 (ERK1/2) protein phosphorylation decreased, which could be reversed by superoxide dismutase (SOD), 1,10-phenanthroline (Phe), the PP2A phosphorylation inhibitors, okadaic acid (OKA) and cantharidin, and the ERK phosphorylation activator nicotine (p < 0.05). Nicotine 302-310 mitogen activated protein kinase 3 Rattus norvegicus 66-72 23871741-0 2013 Long term alterations in synaptic physiology, expression of beta2 nicotinic receptors and ERK1/2 signaling in the hippocampus of rats with prenatal nicotine exposure. Nicotine 148-156 mitogen activated protein kinase 3 Rattus norvegicus 90-96 23871741-8 2013 Moreover, reduced levels of beta2 subunit containing nicotinic receptors and extracellular signal regulated kinase1/2 (ERK1/2) were observed in nicotine exposed rats. Nicotine 144-152 mitogen activated protein kinase 3 Rattus norvegicus 77-117 23871741-8 2013 Moreover, reduced levels of beta2 subunit containing nicotinic receptors and extracellular signal regulated kinase1/2 (ERK1/2) were observed in nicotine exposed rats. Nicotine 144-152 mitogen activated protein kinase 3 Rattus norvegicus 119-125 23871741-9 2013 These results suggest that long lasting alterations in excitatory synaptic physiology, AMPAR synaptic currents and ERK1/2 signaling may serve as the molecular mechanisms for cognitive deficits associated with prenatal nicotine exposure. Nicotine 218-226 mitogen activated protein kinase 3 Rattus norvegicus 115-121 23624270-7 2013 These data suggest that NO promotes nicotine-triggered Ca(2+) transient in PC12 cells to activate possibly CaMKII, leading to sequential phosphorylation of p38 MAPK and ERK1/2. Nicotine 36-44 mitogen activated protein kinase 3 Rattus norvegicus 169-175 23587498-7 2013 Nicotine-dependent activation of the Ca(2+)/IP3/ERK 1/2 intracellular signalling pathway was also evaluated in normal rat intrahepatic cholangiocyte. Nicotine 0-8 mitogen activated protein kinase 3 Rattus norvegicus 48-55 21742048-9 2011 Blockade of NO pathway may be selective target to reduce ERK1/2 phosphorylation via attenuation of the nicotine responses in the CNS. Nicotine 103-111 mitogen activated protein kinase 3 Rattus norvegicus 57-63 21742048-2 2011 In the present study, we demonstrated that nNOS expression enhances the nicotine-triggered activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in nNOS-transfected PC12 (NPC12) cells. Nicotine 72-80 mitogen activated protein kinase 3 Rattus norvegicus 105-146 21742048-2 2011 In the present study, we demonstrated that nNOS expression enhances the nicotine-triggered activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in nNOS-transfected PC12 (NPC12) cells. Nicotine 72-80 mitogen activated protein kinase 3 Rattus norvegicus 148-154 21742048-3 2011 Treatment with nicotine increased the phosphorylation level of ERK1/2 in the NPC12 cells as compared with that in control PC12 cells. Nicotine 15-23 mitogen activated protein kinase 3 Rattus norvegicus 63-69 21742048-5 2011 The nicotine-induced ERK1/2 phosphorylation in PC12 cells was observed by their pretreatment with a NO donor. Nicotine 4-12 mitogen activated protein kinase 3 Rattus norvegicus 21-27 21742048-6 2011 Moreover, the enhancement of nicotine-induced ERK1/2 phosphorylation in the NPC12 cells was regulated by intracellular glutathione levels, but not by the soluble guanylate cyclase-cGMP-protein kinase G signaling. Nicotine 29-37 mitogen activated protein kinase 3 Rattus norvegicus 46-52 21742048-8 2011 Taken together, these findings suggest that nicotine modulates NO-dependent redox condition; the resulting calcium influx, would increase ERK1/2 phosphorylation in nNOS expressing cells. Nicotine 44-52 mitogen activated protein kinase 3 Rattus norvegicus 138-144 23602986-7 2013 Cotinine also increased the phosphorylation of ERK 1/2 in a similar fashion as nicotine. Nicotine 79-87 mitogen activated protein kinase 3 Rattus norvegicus 47-54 18822160-5 2008 Nicotine and H2O2 at these dose and time intervals produced similar levels of malondialdyde (MDA) production and p-ERK1/2 activation. Nicotine 0-8 mitogen activated protein kinase 3 Rattus norvegicus 115-121 20615913-5 2010 Nicotine activated extracellular signal-regulated kinase (ERK) 1/2 in a dose- and time-dependent manner. Nicotine 0-8 mitogen activated protein kinase 3 Rattus norvegicus 19-66 20211606-4 2010 Robust concentration-dependent increase in ERK1/2 phosphorylation was triggered by structurally diverse alpha7 nAChR agonists such as nicotine, choline, GTS-21, SSR-180711A and PNU-282987 in the presence of the positive allosteric modulator (PAM) PNU-120596. Nicotine 134-142 mitogen activated protein kinase 3 Rattus norvegicus 43-49 18822160-9 2008 These results suggest that nicotine- induced mitogenic and functional response in AR42J cells are associated with ERK signaling and increase in reactive oxygen species production. Nicotine 27-35 mitogen activated protein kinase 3 Rattus norvegicus 114-117 18204935-6 2008 Treatment of AR42J cells with nicotine induced p-ERK 1/2 activation as confirmed by Western blot and immunofluorescence imaging of cytoplasmic localization of mitogen-activated protein kinase (MAPK) signals. Nicotine 30-38 mitogen activated protein kinase 3 Rattus norvegicus 49-56 18204935-6 2008 Treatment of AR42J cells with nicotine induced p-ERK 1/2 activation as confirmed by Western blot and immunofluorescence imaging of cytoplasmic localization of mitogen-activated protein kinase (MAPK) signals. Nicotine 30-38 mitogen activated protein kinase 3 Rattus norvegicus 193-197 17068140-4 2007 Short-term nicotine treatment stimulated phosphorylation of p44/42-MAPK, p38-MAPK, and signal transducer and activator of transcription 3. Nicotine 11-19 mitogen activated protein kinase 3 Rattus norvegicus 60-63 17227300-6 2007 RESULTS: Nicotine treatment caused dose-dependent activation of extracellular signal-regulated kinases (ERK1/2), the maxima occurring at 100 micro m and at 3 min after treatment; the response was suppressed by the ERK1/2 inhibitor. Nicotine 9-17 mitogen activated protein kinase 3 Rattus norvegicus 104-110 17227300-6 2007 RESULTS: Nicotine treatment caused dose-dependent activation of extracellular signal-regulated kinases (ERK1/2), the maxima occurring at 100 micro m and at 3 min after treatment; the response was suppressed by the ERK1/2 inhibitor. Nicotine 9-17 mitogen activated protein kinase 3 Rattus norvegicus 214-220 17227300-8 2007 Exposure of cells to 100 microm nicotine for 6 min significantly enhanced both baseline and cholecystokinin-stimulated cell function, and these effects were not affected by treatment with the inhibitor of ERK1/2 but were suppressed by mecamylamine, a nicotinic receptor antagonist. Nicotine 32-40 mitogen activated protein kinase 3 Rattus norvegicus 205-211 17068140-5 2007 However, an additive effect of nicotine pretreatment on insulin stimulation was only observed on p44/42-MAPK. Nicotine 31-39 mitogen activated protein kinase 3 Rattus norvegicus 97-100 17068140-6 2007 The nicotine-induced phosphorylation of p44/42-MAPK and [methyl-(3)H]thymidine incorporation were effectively suppressed by a alpha7-nAChR-selective antagonist, methyllycaconitine, and the phosphorylation of p44/42-MAPK was stimulated by a alpha7-nAChR-specific agonist, GTS21. Nicotine 4-12 mitogen activated protein kinase 3 Rattus norvegicus 40-43 17068140-6 2007 The nicotine-induced phosphorylation of p44/42-MAPK and [methyl-(3)H]thymidine incorporation were effectively suppressed by a alpha7-nAChR-selective antagonist, methyllycaconitine, and the phosphorylation of p44/42-MAPK was stimulated by a alpha7-nAChR-specific agonist, GTS21. Nicotine 4-12 mitogen activated protein kinase 3 Rattus norvegicus 208-211 17068140-8 2007 Interestingly, long-term (48-h) pretreatment with nicotine increased the amount of alpha7-AChR in the plasma membrane and insulin-induced phosphorylation of p44/42-MAPK. Nicotine 50-58 mitogen activated protein kinase 3 Rattus norvegicus 157-160 17068140-9 2007 These results provide the first evidence that acute exposure to nicotine enhances insulin-induced mitogenesis predominantly by affecting the phosphorylation of p44/42-MAPK and that chronic exposure further augments the insulin signal via up-regulation of alpha7-nAChR, which may be crucial for the development and progression of atherosclerosis in large vessels. Nicotine 64-72 mitogen activated protein kinase 3 Rattus norvegicus 160-163 17523180-3 2007 Immunoblots analysis showed that chronic nicotine treatment of hypothyroid rats prevented the reduction in the basal protein levels of adenylyl cyclase I (ACI), mitogen-activated protein kinases [MAPKp44/42 (ERK1/2)], calcium-calmodulin-dependent protein kinase IV (CaMKIV), and cyclic-AMP response element binding protein [CREB; phosphorylated (P-) and total]. Nicotine 41-49 mitogen activated protein kinase 3 Rattus norvegicus 208-214 16888810-0 2007 ERK 1/2 signaling pathway is involved in nicotine-mediated neuroprotection in spinal cord neurons. Nicotine 41-49 mitogen activated protein kinase 3 Rattus norvegicus 0-7 16888810-2 2007 To study these mechanisms, the present study focused on nicotine-mediated modulation of the extracellular regulated kinase 1 and 2 (ERK1/2) pathway in cultured spinal cord neurons. Nicotine 56-64 mitogen activated protein kinase 3 Rattus norvegicus 132-138 16888810-3 2007 Exposure to nicotine (0.1-10 microM) for as short as 1 min markedly upregulated levels of phosphorylated ERK1/2 (pERK1/2) and increased total ERK1/2 activity. Nicotine 12-20 mitogen activated protein kinase 3 Rattus norvegicus 105-111 16888810-3 2007 Exposure to nicotine (0.1-10 microM) for as short as 1 min markedly upregulated levels of phosphorylated ERK1/2 (pERK1/2) and increased total ERK1/2 activity. Nicotine 12-20 mitogen activated protein kinase 3 Rattus norvegicus 114-120 16888810-5 2007 In addition, pre-exposure to U0126, a specific inhibitor of the ERK1/2 signaling, prevented nicotine-mediated anti-apoptotic effects. Nicotine 92-100 mitogen activated protein kinase 3 Rattus norvegicus 64-70 16888810-6 2007 To indicate if treatment with nicotine also can activate ERK1/2 in vivo, a moderate spinal cord injury (SCI) was induced in rats using a weight-drop device and nicotine was injected 2 h post-trauma. Nicotine 30-38 mitogen activated protein kinase 3 Rattus norvegicus 57-63 16888810-8 2007 Results of the present study indicate that the ERK1/2 pathway is involved in anti-apoptotic effects of nicotine in spinal cord neurons and may be involved in therapeutic effects of nicotine in spinal cord trauma. Nicotine 103-111 mitogen activated protein kinase 3 Rattus norvegicus 47-53 16888810-8 2007 Results of the present study indicate that the ERK1/2 pathway is involved in anti-apoptotic effects of nicotine in spinal cord neurons and may be involved in therapeutic effects of nicotine in spinal cord trauma. Nicotine 181-189 mitogen activated protein kinase 3 Rattus norvegicus 47-53 16051920-0 2005 Activation of p-ERK1/2 by nicotine in pancreatic tumor cell line AR42J: effects on proliferation and secretion. Nicotine 26-34 mitogen activated protein kinase 3 Rattus norvegicus 16-22 16051920-1 2005 The objectives of the present study were to determine the effect of nicotine on MAPK signaling and on the proliferation of AR42J cells as well as to assess the relationship between MAPK activation and exocrine secretion in these cells. Nicotine 68-76 mitogen activated protein kinase 3 Rattus norvegicus 80-84 16051920-2 2005 AR42J cells were incubated with nicotine and analyzed for the activation of MAPK by Western blot analysis using their respective antibodies and confirmed by immunohistochemistry. Nicotine 32-40 mitogen activated protein kinase 3 Rattus norvegicus 76-80 16051920-4 2005 Nicotine at a dose of 100 microM induced phospho-ERK1/2 activation maximally in 3 min compared with untreated cells. Nicotine 0-8 mitogen activated protein kinase 3 Rattus norvegicus 49-55 16051920-5 2005 Furthermore, immunofluorescence study confirmed the nicotine-induced increase in translocation of phospho-ERK1/2 to the nucleus. Nicotine 52-60 mitogen activated protein kinase 3 Rattus norvegicus 106-112 16051920-10 2005 We conclude that nicotine treatment induced activation of ERK1/2 and increased the proliferation of AR42J cells. Nicotine 17-25 mitogen activated protein kinase 3 Rattus norvegicus 58-64 16051920-11 2005 The data further indicate that MAPK signaling by nicotine is independent of the secretory response. Nicotine 49-57 mitogen activated protein kinase 3 Rattus norvegicus 31-35 12393268-2 2002 In the present study, we investigated the effects of alpha7nAChR over-expression and nicotine on ERK phosphorylation and N-cadherin expression by comparing 3 groups of cells: PC12 cells transfected with alpha7 subunit cDNA (alpha7pCMV cells); untransfected PC12 cells exposed to 50 microM nicotine (PC12 cells+nicotine); and PC12 cells transfected with vector only (pCMV cells). Nicotine 85-93 mitogen activated protein kinase 3 Rattus norvegicus 97-100 12393268-5 2002 PC12 cells+nicotine exhibited transient expression of phospho-ERKs at 48 h after addition of nicotine, but did not exhibit differentiation-like transformation. Nicotine 11-19 mitogen activated protein kinase 3 Rattus norvegicus 62-66 12393268-5 2002 PC12 cells+nicotine exhibited transient expression of phospho-ERKs at 48 h after addition of nicotine, but did not exhibit differentiation-like transformation. Nicotine 93-101 mitogen activated protein kinase 3 Rattus norvegicus 62-66 16452470-4 2006 In contrast, ERK1/2 was only phosphorylated with short term nicotine exposure. Nicotine 60-68 mitogen activated protein kinase 3 Rattus norvegicus 13-19 16452470-5 2006 MEK inhibitor U0126 abolished nicotine-induced rise in P-ERK1/2, but not P-CREB, nor did it inhibit nicotine-evoked elevation in TH promoter activity, indicating that ERK1/2 was not needed for induction of TH gene expression by nicotine. Nicotine 30-38 mitogen activated protein kinase 3 Rattus norvegicus 57-63 15983034-4 2005 Also, Ras and its downstream effector ERK1/2 are activated after long term exposure to nicotine. Nicotine 87-95 mitogen activated protein kinase 3 Rattus norvegicus 38-44 11749838-3 2001 RESULTS: PD98059 concentration-dependently (25 micromol/L, 50 micromol/L) attenuated the induction of LTP induced by nicotine 10 micromol/L; both p42 and p44 MAPK were activated with their total protein expression increasing in CA1 subregion in response to LTP induced by nicotine. Nicotine 117-125 mitogen activated protein kinase 3 Rattus norvegicus 154-162 11749838-3 2001 RESULTS: PD98059 concentration-dependently (25 micromol/L, 50 micromol/L) attenuated the induction of LTP induced by nicotine 10 micromol/L; both p42 and p44 MAPK were activated with their total protein expression increasing in CA1 subregion in response to LTP induced by nicotine. Nicotine 272-280 mitogen activated protein kinase 3 Rattus norvegicus 154-162