PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34557761-10 2021 These data indicate that TLR4 inhibition by nicotine and cotinine at the concentrations tested in BV-2 cells is independent of classic neuronal nAChRs and validate that MD2 is a direct target of nicotine and cotinine in the inhibition of innate immunity. Nicotine 44-52 toll-like receptor 4 Mus musculus 25-29 34557761-0 2021 Nicotine and its metabolite cotinine target MD2 and inhibit TLR4 signaling. Nicotine 0-8 toll-like receptor 4 Mus musculus 60-64 15451549-1 2004 Nicotine has been shown to induce relaxation via nitric oxide (NO) production with activation of endothelium nitric oxide synthase (eNOS), however the effect of nicotine on lipopolysaccharide/interferon-gamma (LPS/IFN-gamma)-induced NO production and inducible NOS (iNOS) gene expression is still undefined. Nicotine 0-8 toll-like receptor 4 Mus musculus 210-223 15451549-1 2004 Nicotine has been shown to induce relaxation via nitric oxide (NO) production with activation of endothelium nitric oxide synthase (eNOS), however the effect of nicotine on lipopolysaccharide/interferon-gamma (LPS/IFN-gamma)-induced NO production and inducible NOS (iNOS) gene expression is still undefined. Nicotine 161-169 toll-like receptor 4 Mus musculus 210-223 34557761-4 2021 Considering the psychoactive substances morphine, cocaine, and methamphetamine act as xenobiotic-associated molecular patterns and can be specifically sensed by the innate immune receptor Toll-like receptor 4 (TLR4), here we sought to delineate whether nicotine and/or its metabolite cotinine may be recognized by the innate immune system via myeloid differentiation protein 2 (MD2), an accessory protein of TLR4 that is responsible for ligand recognition. Nicotine 253-261 toll-like receptor 4 Mus musculus 188-208 34557761-10 2021 These data indicate that TLR4 inhibition by nicotine and cotinine at the concentrations tested in BV-2 cells is independent of classic neuronal nAChRs and validate that MD2 is a direct target of nicotine and cotinine in the inhibition of innate immunity. Nicotine 195-203 toll-like receptor 4 Mus musculus 25-29 34557761-4 2021 Considering the psychoactive substances morphine, cocaine, and methamphetamine act as xenobiotic-associated molecular patterns and can be specifically sensed by the innate immune receptor Toll-like receptor 4 (TLR4), here we sought to delineate whether nicotine and/or its metabolite cotinine may be recognized by the innate immune system via myeloid differentiation protein 2 (MD2), an accessory protein of TLR4 that is responsible for ligand recognition. Nicotine 253-261 toll-like receptor 4 Mus musculus 210-214 34557761-4 2021 Considering the psychoactive substances morphine, cocaine, and methamphetamine act as xenobiotic-associated molecular patterns and can be specifically sensed by the innate immune receptor Toll-like receptor 4 (TLR4), here we sought to delineate whether nicotine and/or its metabolite cotinine may be recognized by the innate immune system via myeloid differentiation protein 2 (MD2), an accessory protein of TLR4 that is responsible for ligand recognition. Nicotine 253-261 toll-like receptor 4 Mus musculus 408-412 34557761-5 2021 MD2-intrinsic fluorescence titrations, surface plasmon resonance, and competitive displacement binding assays with curcumin (MD2 probe) demonstrated that both nicotine and cotinine targeted the lipopolysaccharide (LPS; TLR4 agonist) binding pocket of MD2 with similar affinities. Nicotine 159-167 toll-like receptor 4 Mus musculus 219-223 28546155-0 2017 Long-term nicotine exposure dampens LPS-induced nerve-mediated airway hyperreactivity in murine airways. Nicotine 10-18 toll-like receptor 4 Mus musculus 36-39 28546155-3 2017 The aim of the present study is to investigate the effect of nicotine on lipopolysaccharide (LPS)-induced airway hyperreactivity (AHR) and to explore the potential involvement of neuronal mechanisms behind nicotine"s effects in murine models in vivo and in vitro. Nicotine 61-69 toll-like receptor 4 Mus musculus 93-96 28546155-9 2017 Interestingly, long-term nicotine exposure markedly dampened this LPS-induced AHR both in vitro and in vivo. Nicotine 25-33 toll-like receptor 4 Mus musculus 66-69 28546155-11 2017 In conclusion, long-term nicotine exposure dampened LPS-induced AHR. Nicotine 25-33 toll-like receptor 4 Mus musculus 52-55 24669857-0 2014 Nicotine exaggerates LPS-induced airway hyperreactivity via JNK-mediated up-regulation of Toll-like receptor 4. Nicotine 0-8 toll-like receptor 4 Mus musculus 90-110 24669857-12 2014 In conclusion, long-term exposure to nicotine up-regulated the expression of TLR4 and -6 via a JNK-related pathway, causing an exaggeration of the LPS-induced local airway inflammation and increased AHR. Nicotine 37-45 toll-like receptor 4 Mus musculus 77-81 27938563-6 2016 The results of Western blot showed that compared with the endotoxin+nicotine group of C57 NASH mice, the endotoxin+nicotine group of gene knockout NASH mice had significantly higher relative protein expression of phosphorylated NF-kappaB and TLR-4 (NF-kappaB: 69 425+-600 vs 51 133+-200, P < 0.05; TLR-4: 93 387+-684 vs 64 198+-630, P < 0.05). Nicotine 115-123 toll-like receptor 4 Mus musculus 242-247 27938563-6 2016 The results of Western blot showed that compared with the endotoxin+nicotine group of C57 NASH mice, the endotoxin+nicotine group of gene knockout NASH mice had significantly higher relative protein expression of phosphorylated NF-kappaB and TLR-4 (NF-kappaB: 69 425+-600 vs 51 133+-200, P < 0.05; TLR-4: 93 387+-684 vs 64 198+-630, P < 0.05). Nicotine 115-123 toll-like receptor 4 Mus musculus 301-306 27938563-8 2016 The results of PCR showed that the endotoxin+nicotine group of gene knockout NASH mice had significantly higher relative mRNA expression of TLR-4 than the endotoxin+nicotine group of C57 NASH mice (4.13+-0.13 vs 2.93+-0.14, P < 0.05). Nicotine 45-53 toll-like receptor 4 Mus musculus 140-145 24669857-10 2014 Nicotine increased mRNA and protein expression of TLR4 and -6 in the epithelium and smooth muscle layer, with MCP-1 and COX-2 mRNA increasing in parallel. Nicotine 0-8 toll-like receptor 4 Mus musculus 50-54 24298024-9 2014 Nicotine enhanced PI3K/Akt activation and reduced PU.1 activity and TLR4 expression. Nicotine 0-8 toll-like receptor 4 Mus musculus 68-72 18059323-5 2008 Cigarette smoke contains a large number of molecules which may mediate responses on immune cells and of these, nicotine and oxidants have both been identified as inhibitory for the sensing of bacterial lipopolysaccharide (LPS). Nicotine 111-119 toll-like receptor 4 Mus musculus 222-225