PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8344304-4 1993 From these last two observations it was concluded that the mechanism by which phospholipids influence P-450-IIB1-dependent O-dealkylation of ethoxycoumarin is different from that by which they influence P-450-IIB1-dependent O-dealkylation of this substrate. Phospholipids 78-91 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 102-112 8619853-3 1996 Using purified cDNA-expressed CYP3A4 incorporated into membranous vesicles made from microsomal phospholipids, rates of nifedipine and testosterone oxidation of about 60 nmol/nmol P450/min were achieved, whereas similar reconstitution into dilauroyl-phosphatidylcholine micelles was unsuccessful. Phospholipids 96-109 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 180-188 7517356-1 1994 Membrane-bound sites of cytochrome P-450 2B4 (LM2) were determined by means of two different methods, photoactivated binding of membrane phospholipids to the protein and epitope mapping by antibodies. Phospholipids 137-150 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 35-40 16415125-6 2006 Using either cholate method, more than 85% of the P450 was physically incorporated into the phospholipid vesicles, whereas less than 40% of the P450 was physically incorporated into the phospholipid vesicles using the SRS. Phospholipids 92-104 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 50-54 10375402-2 1999 Purified human liver microsomal cytochromes P450 (P450)-P450 1A2 and P450 2E1-were shown to have appreciable PLD activity, hydrolyzing phosphatidylcholine but not other phospholipids, generating PA and choline. Phospholipids 169-182 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-48 10375402-2 1999 Purified human liver microsomal cytochromes P450 (P450)-P450 1A2 and P450 2E1-were shown to have appreciable PLD activity, hydrolyzing phosphatidylcholine but not other phospholipids, generating PA and choline. Phospholipids 169-182 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 50-54 10375402-2 1999 Purified human liver microsomal cytochromes P450 (P450)-P450 1A2 and P450 2E1-were shown to have appreciable PLD activity, hydrolyzing phosphatidylcholine but not other phospholipids, generating PA and choline. Phospholipids 169-182 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 50-54 10375402-2 1999 Purified human liver microsomal cytochromes P450 (P450)-P450 1A2 and P450 2E1-were shown to have appreciable PLD activity, hydrolyzing phosphatidylcholine but not other phospholipids, generating PA and choline. Phospholipids 169-182 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 50-54 8460938-7 1993 The monooxygenase activities of the purified human brain P450s were demonstrated with various substrates (aminopyrine, morphine, aniline, 7-ethoxycoumarin, and nifedipine) as examined in reconstituted systems consisting of purified human brain P450, purified rat brain NADPH cytochrome P450 reductase, deoxycholate, phospholipid, and NADPH. Phospholipids 316-328 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 57-61 356063-1 1978 The reviewed kinetic investigations at the experimentally accessible partial reactions of the reaction sequence clearly evidence that the P-450 system represents a complex reaction system, the main control points of which are shown in Scheme 3: As can be seen, control elements are the substrate, the cytochrome species (P-450/448), the reducing agent, the reductase, phospholipid and parts of the b5 system as well. Phospholipids 368-380 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 321-330 23388178-3 2013 Following release from the phospholipid membrane, arachidonic acid can be metabolized into different classes of eicosanoids through cyclooxygenases, lipoxygenases, or p450 epoxygenase pathways. Phospholipids 27-39 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 167-171 17213193-2 2007 Here, we describe the detailed characterization of a functionally homogeneous 1:1 complex of cytochrome P450 3A4 (CYP3A4) and cytochrome P450 reductase solubilized via self-assembly in a nanoscale phospholipid bilayer. Phospholipids 197-209 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 104-108 6489339-0 1984 Mechanism of rate control of the NADPH-dependent reduction of cytochrome P-450 by lipids in reconstituted phospholipid vesicles. Phospholipids 106-118 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 73-78 6489339-1 1984 The NADPH-supported reduction of cytochrome P-450 LM2 (liver microsomal isozyme 2) in reconstituted phospholipid vesicles in general exhibits two-exponential kinetics. Phospholipids 100-112 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-49 24893120-3 2014 This method does not require any protein-tagging system for protein isolation and has a further advantage that the purification is concomitantly conducted with reconstitution of the enzymes into a phospholipid environment, which is crucial for the catalytic activity assay of P450 enzyme. Phospholipids 197-209 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 276-280 17979764-0 2007 Functional regulation of hepatic cytochrome p450 enzymes by physicochemical properties of phospholipids in biological membranes. Phospholipids 90-103 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 44-48 17979764-2 2007 P450 is a membrane-anchored protein that shows a variety of interaction with membrane phospholipids, which affect the membrane topology and catalytic activities of the protein. Phospholipids 86-99 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 0-4 17979764-3 2007 In particular, anionic phospholipids, nonbilayer forming lipids, and the degree of saturation of the lipid fatty acyl chain play important roles in the functional regulation of P450, as well as in the bilayer structure of the membrane. Phospholipids 23-36 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 177-181 17979764-4 2007 However, despite the importance of phospholipids in the regulation of P450s, the interaction of the protein with membrane phospholipids, and the membrane properties induced by phospholipids which regulate P450, are unclear. Phospholipids 35-48 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 70-74 17979764-5 2007 In this review, we describe the effect of the physicochemical properties of the phospholipid constituents of biological membranes on hepatic P450 catalytic activity, membrane insertion (and/or penetration), and structural changes. Phospholipids 80-92 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 141-145