PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18682551-7 2008 Vascular endothelial growth factor-induced activation of Akt increased NOx in treated (50 nM DHA) versus untreated HCAEC (9.2 +/- 1.0 vs 3.3 +/- 1.1 micromol/microg protein/microL). nicotine 1-N-oxide 71-74 AKT serine/threonine kinase 1 Homo sapiens 57-60 22728270-8 2012 Moreover, by using the PI3K/AKT inhibitor wortmannin or shRNA of AKT1, the TGIF-induced Nox activation and superoxide production were significantly inhibited. nicotine 1-N-oxide 88-91 AKT serine/threonine kinase 1 Homo sapiens 28-31 22728270-8 2012 Moreover, by using the PI3K/AKT inhibitor wortmannin or shRNA of AKT1, the TGIF-induced Nox activation and superoxide production were significantly inhibited. nicotine 1-N-oxide 88-91 AKT serine/threonine kinase 1 Homo sapiens 65-69 25730848-10 2015 Unlike NOX-dependent NETosis, NOX-independent NETosis is accompanied by a substantially lower level of activation of ERK and moderate level of activation of Akt, whereas the activation of p38 is similar in both pathways. nicotine 1-N-oxide 30-33 AKT serine/threonine kinase 1 Homo sapiens 157-160 25730848-12 2015 Despite the differential activation, both NOX-dependent and -independent NETosis require Akt activity. nicotine 1-N-oxide 42-45 AKT serine/threonine kinase 1 Homo sapiens 89-92 7568183-3 1995 These include the yeast SEC6, SEC8, and SEC15 genes, whose products are constituents of a 19.5S particle that interacts with the GTP-binding protein Sec4p. Guanosine Triphosphate 129-132 Rab GTPase-binding exocyst subunit SEC15 Saccharomyces cerevisiae S288C 40-45 7568183-3 1995 These include the yeast SEC6, SEC8, and SEC15 genes, whose products are constituents of a 19.5S particle that interacts with the GTP-binding protein Sec4p. Guanosine Triphosphate 129-132 Rab family GTPase SEC4 Saccharomyces cerevisiae S288C 149-154 7565673-4 1995 Cdc42p is required for alpha-factor-induced activation of FUS1.cdc24ts strains defective for Cdc42p GDP/GTP exchange show no pheromone induction at restrictive temperatures but are partially rescued by overexpression of Cdc42p, which is potentiated by Cdc42p12V mutants. Guanosine Triphosphate 104-107 Fus1p Saccharomyces cerevisiae S288C 58-62 7547927-1 1995 The small GTP-binding protein G25K and the protein K-Ras 4B contain prenyl groups (geranylgeranyl and farnesyl, respectively) that are thioether linked to a C-terminal cysteine which is methylated on its alpha-carboxyl group. Guanosine Triphosphate 10-13 cell division cycle 42 Homo sapiens 30-34 6736200-10 1984 Dose-response curves showed that the apparent activation constants for GTP and GMP-P(NH)P were 0.30 and 0.51 microM, respectively; these values did not shift after the addition of hCG. Guanosine Triphosphate 71-74 chorionic gonadotropin subunit beta 5 Homo sapiens 180-183 7573382-10 1995 Adenosine, through the activation of a GTP-binding protein, antagonizes the ATPi-induced channel closure. Guanosine Triphosphate 39-42 ATP synthase inhibitory factor subunit 1 Homo sapiens 76-80 8673720-0 1995 Intracellular transport and maturation of nascent low density lipoprotein receptor is blocked by mutation in the Ras-related GTP-binding protein, RAB1B. Guanosine Triphosphate 125-128 RAB1B, member RAS oncogene family Homo sapiens 146-151 6325458-1 1984 Transcription of the yeast mitochondrial var 1 gene encoding the mitochondrial small ribosomal subunit protein, var 1, has been examined in wild type and petite strains by Northern hybridization, S 1 nuclease protection experiments, and analysis of primary transcripts by in vitro labeling of mitochondrial RNAs with [alpha-32P]GTP and guanylyl transferase. Guanosine Triphosphate 328-331 mitochondrial 37S ribosomal protein VAR1 Saccharomyces cerevisiae S288C 41-46 6325458-1 1984 Transcription of the yeast mitochondrial var 1 gene encoding the mitochondrial small ribosomal subunit protein, var 1, has been examined in wild type and petite strains by Northern hybridization, S 1 nuclease protection experiments, and analysis of primary transcripts by in vitro labeling of mitochondrial RNAs with [alpha-32P]GTP and guanylyl transferase. Guanosine Triphosphate 328-331 mitochondrial 37S ribosomal protein VAR1 Saccharomyces cerevisiae S288C 112-117 7664746-4 1995 From these observations we proposed that a second eRF should exist, conferring GTP dependence for translation termination. Guanosine Triphosphate 79-82 ETS2 repressor factor S homeolog Xenopus laevis 50-53 6609366-6 1984 In vitro, the valine form of p21 had 2.4- and 2.7-fold greater autophosphorylating activity than the glycine and arginine forms of p21, respectively, using [gamma-32P]GTP as phosphate donor, but the three p21 species had similar Km values for GTP (0.20-0.27 microM). Guanosine Triphosphate 167-170 transcription elongation factor A like 1 Homo sapiens 29-32 7664746-5 1995 Here, we have shown that the newly sequenced GTP binding Sup35-like protein from Xenopus laevis, termed eRF3, exhibits in vitro three important functional properties: (i) although being inactive as an eRF on its own, it greatly stimulates eRF1 activity in the presence of GTP and low concentrations of stop codons, resembling the properties of prokaryotic RF3; (ii) it binds and probably hydrolyses GTP; and (iii) it binds to eRF1. Guanosine Triphosphate 45-48 ETS2 repressor factor S homeolog Xenopus laevis 104-107 6609366-6 1984 In vitro, the valine form of p21 had 2.4- and 2.7-fold greater autophosphorylating activity than the glycine and arginine forms of p21, respectively, using [gamma-32P]GTP as phosphate donor, but the three p21 species had similar Km values for GTP (0.20-0.27 microM). Guanosine Triphosphate 167-170 transcription elongation factor A like 1 Homo sapiens 131-134 6609366-6 1984 In vitro, the valine form of p21 had 2.4- and 2.7-fold greater autophosphorylating activity than the glycine and arginine forms of p21, respectively, using [gamma-32P]GTP as phosphate donor, but the three p21 species had similar Km values for GTP (0.20-0.27 microM). Guanosine Triphosphate 167-170 transcription elongation factor A like 1 Homo sapiens 131-134 6609366-6 1984 In vitro, the valine form of p21 had 2.4- and 2.7-fold greater autophosphorylating activity than the glycine and arginine forms of p21, respectively, using [gamma-32P]GTP as phosphate donor, but the three p21 species had similar Km values for GTP (0.20-0.27 microM). Guanosine Triphosphate 243-246 transcription elongation factor A like 1 Homo sapiens 29-32 7664746-5 1995 Here, we have shown that the newly sequenced GTP binding Sup35-like protein from Xenopus laevis, termed eRF3, exhibits in vitro three important functional properties: (i) although being inactive as an eRF on its own, it greatly stimulates eRF1 activity in the presence of GTP and low concentrations of stop codons, resembling the properties of prokaryotic RF3; (ii) it binds and probably hydrolyses GTP; and (iii) it binds to eRF1. Guanosine Triphosphate 272-275 ETS2 repressor factor S homeolog Xenopus laevis 104-107 7664746-5 1995 Here, we have shown that the newly sequenced GTP binding Sup35-like protein from Xenopus laevis, termed eRF3, exhibits in vitro three important functional properties: (i) although being inactive as an eRF on its own, it greatly stimulates eRF1 activity in the presence of GTP and low concentrations of stop codons, resembling the properties of prokaryotic RF3; (ii) it binds and probably hydrolyses GTP; and (iii) it binds to eRF1. Guanosine Triphosphate 272-275 ETS2 repressor factor S homeolog Xenopus laevis 104-107 7653591-2 1995 RhoA, CDC42, and Rac1 small GTP-binding proteins were found to be expressed at high levels in rat outer kidney cortex. Guanosine Triphosphate 28-31 Rac family small GTPase 1 Rattus norvegicus 17-21 8520487-1 1995 Computer-assisted analysis of amino acid sequences using methods for database screening with individual sequences and with multiple alignment blocks reveals a complex multidomain organization of yeast proteins GCD6 and GCD1, and mammalian homolog of GCD6-subunits of the eukaryotic translation initiation factor eIF-2B involved in GDP/GTP exchange on eIF-2. Guanosine Triphosphate 335-338 translation initiation factor eIF2B subunit gamma Saccharomyces cerevisiae S288C 219-223 6142822-3 1984 The IAP treatment, which caused a loss of GTP and hormone-induced adenylate cyclase inhibition, did not prevent enzyme inhibition by the stable GTP analogue. Guanosine Triphosphate 42-45 Cd47 molecule Rattus norvegicus 4-7 6319133-3 1984 ATP was the optimum substrate for the tonoplast ATPase, but there was also evidence for tonoplast-bound GDP-hydrolyzing and GTP-hydrolyzing enzymes which can interfere with the ATPase assay. Guanosine Triphosphate 124-127 dynein axonemal heavy chain 8 Homo sapiens 177-183 7619808-1 1995 The Sec4/Ypt1/Rab family of small GTP-binding proteins are involved in the regulation of intracellular vesicular transport. Guanosine Triphosphate 34-37 RAB1A, member RAS oncogene family Homo sapiens 9-13 6323160-5 1984 The Ala-375 replacements also lower the dissociation rates of the binary complexes EF-Tu.GTP and the binding constants for EF-Tu.GTP and Phe-tRNA. Guanosine Triphosphate 89-92 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 83-88 7619808-1 1995 The Sec4/Ypt1/Rab family of small GTP-binding proteins are involved in the regulation of intracellular vesicular transport. Guanosine Triphosphate 34-37 RAB1A, member RAS oncogene family Homo sapiens 14-17 6323160-5 1984 The Ala-375 replacements also lower the dissociation rates of the binary complexes EF-Tu.GTP and the binding constants for EF-Tu.GTP and Phe-tRNA. Guanosine Triphosphate 129-132 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 83-88 7576990-5 1995 This GTP-bound form of p21ras interacts with the protein kinase raf1 and induces the activation of a kinase cascade, resulting in various cellular responses. Guanosine Triphosphate 5-8 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 64-68 6323160-5 1984 The Ala-375 replacements also lower the dissociation rates of the binary complexes EF-Tu.GTP and the binding constants for EF-Tu.GTP and Phe-tRNA. Guanosine Triphosphate 129-132 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 123-128 6323160-10 1984 It increases the dissociation rate of EF-Tu.GTP by approximately 30%. Guanosine Triphosphate 44-47 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 38-43 7551035-1 1995 The mob locus of Escherichia coli encodes functions which catalyse the synthesis of active molybdenum cofactor, molybdopterin guanine dinucleotide, from molybdopterin and GTP. Guanosine Triphosphate 171-174 mobilization protein Escherichia coli 4-7 6141763-2 1983 The first kinase, present in greatest activity in microsomal extracts, appears to be identical to casein kinase I by characteristic molecular size on gel filtration (Mr 40,000) and sodium dodecyl sulfate-gel electrophoresis (Mr 34,000), autophosphorylation of this single subunit, inability to efficiently utilize GTP, and resistance to inhibition by heparin and 2,3-diphosphoglycerate. Guanosine Triphosphate 314-317 casein kinase 1, epsilon Rattus norvegicus 98-113 7796906-2 1995 Upon exchange of bound GDP for GTP in the SCG1 subunit, the release of STE4/STE18 dimer occurs which, in turn causes activation of downstream effectors leading growth arrest and mating competence. Guanosine Triphosphate 31-34 guanine nucleotide-binding protein subunit alpha Saccharomyces cerevisiae S288C 42-46 6683482-2 1983 IAP and its A protomer were equipotent, on a molar basis, in enhancing GTP-dependent adenylate cyclase activity and in causing ADP-ribosylation of the 41,000 Mr protein when directly added to the cell-free membrane preparation from rat C6 glioma cells. Guanosine Triphosphate 71-74 Cd47 molecule Rattus norvegicus 0-3 7597098-4 1995 Our results lead us to propose that signaling modules composed of small GTP-binding proteins and protein kinases related to Shk1, Ste20, and p65PAK, are highly conserved in evolution and participate in both cytoskeletal functions and mitogen-activated protein kinase signaling pathways. Guanosine Triphosphate 72-75 p21 (RAC1) activated kinase 1 Homo sapiens 141-147 6277948-2 1982 Both the increase in the cellular cAMP content in response to a beta-adrenergic agonist and the stimulation of membrane adenylate cyclase by the beta-agonist and/or GTP were markedly enhanced by the IAP treatment of C6 cells, but no change was induced in affinities of the agonist (or an antagonist) or GTP for their respective sites of action (or binding). Guanosine Triphosphate 165-168 islet amyloid polypeptide Homo sapiens 199-202 7794902-0 1995 Macromolecular arrangement in the aminoacyl-tRNA.elongation factor Tu.GTP ternary complex. Guanosine Triphosphate 70-73 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 49-69 6277948-2 1982 Both the increase in the cellular cAMP content in response to a beta-adrenergic agonist and the stimulation of membrane adenylate cyclase by the beta-agonist and/or GTP were markedly enhanced by the IAP treatment of C6 cells, but no change was induced in affinities of the agonist (or an antagonist) or GTP for their respective sites of action (or binding). Guanosine Triphosphate 303-306 islet amyloid polypeptide Homo sapiens 199-202 6277948-6 1982 Thus, IAP would appear to enhance beta-receptor-coupled stimulation of adenylate cyclase, in a manner distinct from cholera toxin, by rendering more GTP available to the GTP sites on the regulatory subunit of the receptor-enzyme system. Guanosine Triphosphate 149-152 islet amyloid polypeptide Homo sapiens 6-9 6277948-6 1982 Thus, IAP would appear to enhance beta-receptor-coupled stimulation of adenylate cyclase, in a manner distinct from cholera toxin, by rendering more GTP available to the GTP sites on the regulatory subunit of the receptor-enzyme system. Guanosine Triphosphate 170-173 islet amyloid polypeptide Homo sapiens 6-9 7794902-2 1995 The distance between the corner of the L-shaped transfer RNA and the GTP bound to elongation factor Tu (EF-Tu) in the aminoacyl-tRNA.EF-Tu.GTP ternary complex was measured using fluorescence energy transfer. Guanosine Triphosphate 69-72 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 82-102 7794902-2 1995 The distance between the corner of the L-shaped transfer RNA and the GTP bound to elongation factor Tu (EF-Tu) in the aminoacyl-tRNA.EF-Tu.GTP ternary complex was measured using fluorescence energy transfer. Guanosine Triphosphate 69-72 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 104-109 6277921-6 1982 AMP, ADP, Ap4, GTP, Gp4, Ap3A, Ap5A, Gp3G, and Gp5G are noncompetitive inhibitors of the Ap4A hydrolase activity, whereas Gp4G inhibits Ap4A hydrolysis competitively with a Ki of 6 microM. Guanosine Triphosphate 15-18 nudix (nucleoside diphosphate linked moiety X)-type motif 2 Mus musculus 89-103 7794902-2 1995 The distance between the corner of the L-shaped transfer RNA and the GTP bound to elongation factor Tu (EF-Tu) in the aminoacyl-tRNA.EF-Tu.GTP ternary complex was measured using fluorescence energy transfer. Guanosine Triphosphate 69-72 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 133-138 7794902-2 1995 The distance between the corner of the L-shaped transfer RNA and the GTP bound to elongation factor Tu (EF-Tu) in the aminoacyl-tRNA.EF-Tu.GTP ternary complex was measured using fluorescence energy transfer. Guanosine Triphosphate 139-142 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 82-102 7794902-2 1995 The distance between the corner of the L-shaped transfer RNA and the GTP bound to elongation factor Tu (EF-Tu) in the aminoacyl-tRNA.EF-Tu.GTP ternary complex was measured using fluorescence energy transfer. Guanosine Triphosphate 139-142 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 104-109 7794902-2 1995 The distance between the corner of the L-shaped transfer RNA and the GTP bound to elongation factor Tu (EF-Tu) in the aminoacyl-tRNA.EF-Tu.GTP ternary complex was measured using fluorescence energy transfer. Guanosine Triphosphate 139-142 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 133-138 7539429-5 1995 Purified His-MxA exhibited specific GTP hydrolysis rates of up to 350 nmol of GTP/min/mg of protein, corresponding to a turnover number of 27 min-1. Guanosine Triphosphate 36-39 MX dynamin like GTPase 1 Homo sapiens 13-16 7056233-0 1982 D-2 dopamine receptor-mediated inhibition of adenylate cyclase activity in the intermediate lobe of the rat pituitary gland requires guanosine 5"-triphosphate. Guanosine Triphosphate 133-158 dopamine receptor D2 Rattus norvegicus 0-21 7539429-5 1995 Purified His-MxA exhibited specific GTP hydrolysis rates of up to 350 nmol of GTP/min/mg of protein, corresponding to a turnover number of 27 min-1. Guanosine Triphosphate 78-81 MX dynamin like GTPase 1 Homo sapiens 13-16 7539429-9 1995 Competitive binding studies with nonlabeled nucleotides revealed a similar binding preference of His-MxA for GTP over GDP: the Kd for GTP was 20 microM, whereas the Kd for GDP was 100 microM. Guanosine Triphosphate 109-112 MX dynamin like GTPase 1 Homo sapiens 101-104 7539429-9 1995 Competitive binding studies with nonlabeled nucleotides revealed a similar binding preference of His-MxA for GTP over GDP: the Kd for GTP was 20 microM, whereas the Kd for GDP was 100 microM. Guanosine Triphosphate 134-137 MX dynamin like GTPase 1 Homo sapiens 101-104 7539429-10 1995 Thus, a high percentage of MxA molecules may be complexed with GTP in vivo. Guanosine Triphosphate 63-66 MX dynamin like GTPase 1 Homo sapiens 27-30 7539430-3 1995 MxA mutants with COOH-terminal deletions of 63 or more amino acids lost all ability to hydrolyze GTP and failed to bind guanine nucleotides. Guanosine Triphosphate 97-100 MX dynamin like GTPase 1 Homo sapiens 0-3