PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 31989565-2 2020 Here, we describe a high-throughput method for identifying mouse spleen lymphocytes with mutations in the endogenous X-linked hypoxanthine guanine phosphoribosyl transferase (Hprt) gene and the endogenous autosomal thymidine kinase (Tk) gene. Hypoxanthine 126-161 hypoxanthine guanine phosphoribosyl transferase Mus musculus 175-179 15159647-1 2004 Hypoxanthine-guanine phosphoribosyltransferase (HPRT) is an enzyme that catalyses the conversion of hypoxanthine and guanine into their respective nucleotides. Hypoxanthine 100-112 hypoxanthine guanine phosphoribosyl transferase Mus musculus 0-46 19391117-4 2009 Homologous recombination into the Hprt locus, which is deleted for the promoter and first two exons in HM1 embryonic stem cells, reconstitutes a functional Hprt gene, allowing for growth in HAT (hypoxanthine-aminopterin-thymidine) medium. Hypoxanthine 195-207 hypoxanthine guanine phosphoribosyl transferase Mus musculus 34-38 19391117-4 2009 Homologous recombination into the Hprt locus, which is deleted for the promoter and first two exons in HM1 embryonic stem cells, reconstitutes a functional Hprt gene, allowing for growth in HAT (hypoxanthine-aminopterin-thymidine) medium. Hypoxanthine 195-207 hypoxanthine guanine phosphoribosyl transferase Mus musculus 156-160 15159647-1 2004 Hypoxanthine-guanine phosphoribosyltransferase (HPRT) is an enzyme that catalyses the conversion of hypoxanthine and guanine into their respective nucleotides. Hypoxanthine 100-112 hypoxanthine guanine phosphoribosyl transferase Mus musculus 48-52 7027497-5 1981 HPRT extracted from NBR4 had decreased affinity of both hypoxanthine and PRPP relative to wild type. Hypoxanthine 56-68 hypoxanthine guanine phosphoribosyl transferase Mus musculus 0-4 12630412-2 2002 After 5 days of coculture, HPRT-deficient ES cells were killed by selection in HAT (hypoxanthine, aminopterin, thymidine) medium. Hypoxanthine 84-96 hypoxanthine guanine phosphoribosyl transferase Mus musculus 27-31 1873380-0 1991 Hypoxanthine-maintained two-cell block in mouse embryos: dependence on glucose and effect of hypoxanthine phosphoribosyltransferase inhibitors. Hypoxanthine 0-12 hypoxanthine guanine phosphoribosyl transferase Mus musculus 93-131 1873380-6 1991 Assays of hypoxanthine phosphoribosyltransferase (HPRT) activity in lysates of four-cell embryos determined that the drugs 6-mercapto-9-(tetrahydro-2-furyl)-purine (MPTF) and 6-mercaptopurine (6-MP), but not 6-azauridine (6-AzaU), prevented salvage of hypoxanthine. Hypoxanthine 10-22 hypoxanthine guanine phosphoribosyl transferase Mus musculus 50-54 3622635-7 1987 The HGPRTase-deficient cells were capable of transporting hypoxanthine at increased rates but did not accumulate the base to concentrations in excess of that in the culture medium. Hypoxanthine 58-70 hypoxanthine guanine phosphoribosyl transferase Mus musculus 4-12 3569637-2 1987 This may be due to salvaging guanine by the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT), or to competition for a common membrane carrier involved in mediated transport of both guanine and hypoxanthine in normal biosynthesis and also of MPA. Hypoxanthine 51-63 hypoxanthine guanine phosphoribosyl transferase Mus musculus 100-105 10854040-1 2000 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8.; HPRT) catalyzes the salvage synthesis of inosine-5"-monophosphate (IMP) and guanosine-5"-monophosphate (GMP) from the purine bases hypoxanthine and guanine, respectively. Hypoxanthine 192-204 hypoxanthine guanine phosphoribosyl transferase Mus musculus 0-46 10854040-1 2000 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8.; HPRT) catalyzes the salvage synthesis of inosine-5"-monophosphate (IMP) and guanosine-5"-monophosphate (GMP) from the purine bases hypoxanthine and guanine, respectively. Hypoxanthine 192-204 hypoxanthine guanine phosphoribosyl transferase Mus musculus 61-65 8602176-3 1996 T lymphocytes from spleen were cloned in vitro and HPRT deficient clones were detected by double-labeling with [3H]thymidine and [14C]hypoxanthine. Hypoxanthine 134-146 hypoxanthine guanine phosphoribosyl transferase Mus musculus 51-55 7935410-3 1994 For the second step, a targeting construct (the "plug") that recombines homologously with the integrated socket and supplies the remaining portion of the HPRT minigene is used; this homologous recombination generates a functional HPRT gene and makes the ES cells hypoxanthine-aminopterin-thymidine resistant. Hypoxanthine 263-275 hypoxanthine guanine phosphoribosyl transferase Mus musculus 230-234 8099493-3 1993 Four putative inhibitors of the enzyme, hypoxanthine phosphoribosyltransferase (HPRT), which metabolizes hypoxanthine to inosine monophosphate, were tested on lysates of oocyte-cumulus cell complexes. Hypoxanthine 40-52 hypoxanthine guanine phosphoribosyl transferase Mus musculus 80-84 8099493-7 1993 Also, oocytes from XO mice were more sensitive to the meiosis-arresting action of hypoxanthine than oocytes from XX littermates, which have twice the HPRT activity. Hypoxanthine 82-94 hypoxanthine guanine phosphoribosyl transferase Mus musculus 150-154 8099493-8 1993 The actions of the HPRT inhibitors were not due to their conversion to nucleotides via HPRT and negative feedback on purine de novo synthesis, because azaserine and 6-methylmercaptopurine riboside, which are more potent inhibitors of de novo synthesis, had a stimulatory, rather than inhibitory, effect on hypoxanthine-arrested oocytes. Hypoxanthine 306-318 hypoxanthine guanine phosphoribosyl transferase Mus musculus 19-23 282949-2 1978 Hypoxanthine--guanine phosphoribosyltransferase (HGPRT) activity was measured in erythrocyte haemolysates and quadriceps muscle extracts of normal and dystrophic 129 ReJ and C57 BL/6J mice with [8(-14)C]hypoxanthine as substrate and 5-phosphorylribose 1-pyrophosphate as a ribose 5-phosphate donor. Hypoxanthine 203-215 hypoxanthine guanine phosphoribosyl transferase Mus musculus 0-47 282949-2 1978 Hypoxanthine--guanine phosphoribosyltransferase (HGPRT) activity was measured in erythrocyte haemolysates and quadriceps muscle extracts of normal and dystrophic 129 ReJ and C57 BL/6J mice with [8(-14)C]hypoxanthine as substrate and 5-phosphorylribose 1-pyrophosphate as a ribose 5-phosphate donor. Hypoxanthine 203-215 hypoxanthine guanine phosphoribosyl transferase Mus musculus 49-54 18484-2 1977 These hypoxanthine-aminopterin-thymidine resistant revertant clone had 45-55% of wild-type cell HGPRT activity. Hypoxanthine 6-18 hypoxanthine guanine phosphoribosyl transferase Mus musculus 96-101 18484-4 1977 Apparent Km values of HGPRT for hypoxanthine and 5-phosphoribosyl-1-pyrophosphate were similar in wild-type and revertant cells. Hypoxanthine 32-44 hypoxanthine guanine phosphoribosyl transferase Mus musculus 22-27 560213-3 1977 Mutant cells are resistant to 6-thioguanine at 33-39 degrees C and sensitive to hypoxanthine-aminopterin-thymidine at 37-39 degrees C, but not at 33 degrees C. We hypothesize that a single structural mutation of HPRT can explain these results. Hypoxanthine 80-92 hypoxanthine guanine phosphoribosyl transferase Mus musculus 212-216