PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15990111-1 2005 Human hypoxanthine-guanine phosphoribosyltransferase (HGPRT) catalyses the synthesis of the purine nucleoside monophosphates, IMP and GMP, by the addition of a 6-oxopurine base, either hypoxanthine or guanine, to the 1-beta-position of 5-phospho-alpha-d-ribosyl-1-pyrophosphate (PRib-PP). Hypoxanthine 160-171 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 6-52 15990111-1 2005 Human hypoxanthine-guanine phosphoribosyltransferase (HGPRT) catalyses the synthesis of the purine nucleoside monophosphates, IMP and GMP, by the addition of a 6-oxopurine base, either hypoxanthine or guanine, to the 1-beta-position of 5-phospho-alpha-d-ribosyl-1-pyrophosphate (PRib-PP). Hypoxanthine 160-171 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 54-59 15990111-1 2005 Human hypoxanthine-guanine phosphoribosyltransferase (HGPRT) catalyses the synthesis of the purine nucleoside monophosphates, IMP and GMP, by the addition of a 6-oxopurine base, either hypoxanthine or guanine, to the 1-beta-position of 5-phospho-alpha-d-ribosyl-1-pyrophosphate (PRib-PP). Hypoxanthine 6-18 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 54-59 12829005-1 2003 Lesch-Nyhan syndrome (LNS) is caused by a severe deficiency of hypoxanthine-guanine phosphoribosyltransferase (HPRT) and clinically characterized by self-injurious behavior and nephrolithiasis; the latter is treatable with allopurinol, an inhibitor of xanthine oxidase which converts xanthine and hypoxanthine into uric acid. Hypoxanthine 63-75 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 111-115 15571222-4 2004 The partial HPRT deficiency could be detected only by measuring HPRT activity in intact fibroblasts (uptake of hypoxanthine into nucleotides). Hypoxanthine 111-123 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 12-16 15571295-5 2004 At a fixed concentration of MPA a higher percentage of apoptotic or differentiated cells is obtained when non dialysed serum substitutes for the dialysed one, due to the higher hypoxanthine concentration in the former (about 10 microM) leading to competition on HPRT-mediated salvage of guanine. Hypoxanthine 177-189 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 262-266 1487231-1 1992 Hypoxanthine-guanine phosphoribosyltransferase (HPRT, EC 2.4.2.8) is a purine salvage enzyme that catalyses the conversion of hypoxanthine and guanine to their respective mononucleotides. Hypoxanthine 126-138 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 10833458-1 2000 Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) catalyzes the phosphoribosylation of hypoxanthine and guanine by transferring the phosphoribosyl moiety from phosphoribosylpyrophosphate (PRPP) on to N9 in the purine base, resulting in the formation of inosine monophosphate (IMP) and guanosine monophosphate (GMP). Hypoxanthine 92-104 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 10833458-1 2000 Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) catalyzes the phosphoribosylation of hypoxanthine and guanine by transferring the phosphoribosyl moiety from phosphoribosylpyrophosphate (PRPP) on to N9 in the purine base, resulting in the formation of inosine monophosphate (IMP) and guanosine monophosphate (GMP). Hypoxanthine 92-104 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-53 10657589-2 2000 The underlying HPRT mutation was unique in that the specific activity of HPRT in erythrocyte and in fibroblast lysates was normal, but the rate of uptake of hypoxanthine into nucleotides of intact cultured fibroblasts was markedly reduced (23% of normal). Hypoxanthine 157-169 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 15-19 10657589-3 2000 The low functioning of HPRT in the intact fibroblasts was associated with decreased utilization of endogenously generated hypoxanthine and with decreased utilization of the cosubstrate 5-phosphoribosyl-1-pyrophosphate (PRPP). Hypoxanthine 122-134 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 23-27 9670994-2 1998 The syndrome is caused by a defect in the enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT), which converts guanine and hypoxanthine to the nucleotides GMP and IMP. Hypoxanthine 49-61 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 97-101 8976093-1 1996 The purine salvage enzyme hypoxanthine guanine phosphoribosyl transferase (HPRT) catalyzes the transfer of phosphoribose from PRPP to the 9-position of hypoxanthine or guanine, yielding IMP or GMP, respectively. Hypoxanthine 26-38 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 75-79 8128888-5 1993 The higher activity of HPRT with training suggests an improved potential for rephosphorylation of intracellular hypoxanthine to inosine monophosphate (IMP) in the trained muscle. Hypoxanthine 112-124 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 23-27 11562934-1 2001 Heterozygous carriers of HPRT1 mutations responsible for Lesch-Nyhan syndrome can be detected by analysis of somatic cell hybrids derived from peripheral blood lymphocytes and Hprt1-negative cells of rodent origin followed by selection in culture medium containing hypoxanthine, aminopterine, and thymidine (HAT). Hypoxanthine 265-277 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 25-30 11241585-2 2001 The deficiency of hypoxanthine-guanine phosphoribosyl transferase (HGPRT) in LNS leads to increased hypoxanthine and uric acid production. Hypoxanthine 18-30 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 67-72 11307586-2 2001 The enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) catalyzes the reutilization of hypoxanthine and guanine to the purine nucleotides IMP and GMP, respectively. Hypoxanthine 11-23 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 59-63 10976110-10 2000 Thus, by increasing the binding affinity of 6-oxopurine, we were able to convert the GPRTase to a HGPRTase. Hypoxanthine 44-55 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 98-106 10850548-6 1999 Here we show that infection of human 1306, HPRT-negative cells with RAd-HPRT, expressed high enough levels of HPRT enzyme activity, as to reverse their abnormal biochemical phenotype, thus enhancing hypoxanthine incorporation and restoring purine recycling, increasing GTP levels, decreasing adenine incorporation, and allowing cell survival in HAT medium in which only cells expressing high levels of HPRT can survive. Hypoxanthine 199-211 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 43-47 10850548-6 1999 Here we show that infection of human 1306, HPRT-negative cells with RAd-HPRT, expressed high enough levels of HPRT enzyme activity, as to reverse their abnormal biochemical phenotype, thus enhancing hypoxanthine incorporation and restoring purine recycling, increasing GTP levels, decreasing adenine incorporation, and allowing cell survival in HAT medium in which only cells expressing high levels of HPRT can survive. Hypoxanthine 199-211 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 72-76 10850548-6 1999 Here we show that infection of human 1306, HPRT-negative cells with RAd-HPRT, expressed high enough levels of HPRT enzyme activity, as to reverse their abnormal biochemical phenotype, thus enhancing hypoxanthine incorporation and restoring purine recycling, increasing GTP levels, decreasing adenine incorporation, and allowing cell survival in HAT medium in which only cells expressing high levels of HPRT can survive. Hypoxanthine 199-211 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 72-76 10850548-6 1999 Here we show that infection of human 1306, HPRT-negative cells with RAd-HPRT, expressed high enough levels of HPRT enzyme activity, as to reverse their abnormal biochemical phenotype, thus enhancing hypoxanthine incorporation and restoring purine recycling, increasing GTP levels, decreasing adenine incorporation, and allowing cell survival in HAT medium in which only cells expressing high levels of HPRT can survive. Hypoxanthine 199-211 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 72-76 9860824-1 1998 Hypoxanthine phosphoribosyltransferase (HPRT) salvages 6-oxopurine bases in the nucleotide metabolic pathway. Hypoxanthine 55-66 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-38 9860824-1 1998 Hypoxanthine phosphoribosyltransferase (HPRT) salvages 6-oxopurine bases in the nucleotide metabolic pathway. Hypoxanthine 55-66 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 40-44 9860824-2 1998 The 1.8 A crystal structure of an asymmetric dimer of the HPRT from the protozoan parasite Trypanosoma cruzi was determined in a ternary complex with the primary substrate phosphoribosylpyrophosphate (PRPP) and an analogue of the substrate hypoxanthine, revealing both open and closed active site conformations. Hypoxanthine 240-252 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 58-62 9521733-1 1998 Hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) catalyzes the reversible formation of IMP and GMP from their respective bases hypoxanthine (Hx) and guanine (Gua) and the phosphoribosyl donor 5-phosphoribosyl-1-pyrophosphate (PRPP). Hypoxanthine 136-148 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 9521733-1 1998 Hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) catalyzes the reversible formation of IMP and GMP from their respective bases hypoxanthine (Hx) and guanine (Gua) and the phosphoribosyl donor 5-phosphoribosyl-1-pyrophosphate (PRPP). Hypoxanthine 136-148 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-56 9521733-1 1998 Hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) catalyzes the reversible formation of IMP and GMP from their respective bases hypoxanthine (Hx) and guanine (Gua) and the phosphoribosyl donor 5-phosphoribosyl-1-pyrophosphate (PRPP). Hypoxanthine 150-152 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 9521733-1 1998 Hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) catalyzes the reversible formation of IMP and GMP from their respective bases hypoxanthine (Hx) and guanine (Gua) and the phosphoribosyl donor 5-phosphoribosyl-1-pyrophosphate (PRPP). Hypoxanthine 150-152 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-56 8032345-2 1994 In enzyme pattern-targeted chemotherapy, tiazofurin inhibits IMP dehydrogenase activity in cancer cells and allopurinol-induced high serum hypoxanthine levels inhibit HGPRT activity. Hypoxanthine 139-151 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 167-172 1487231-1 1992 Hypoxanthine-guanine phosphoribosyltransferase (HPRT, EC 2.4.2.8) is a purine salvage enzyme that catalyses the conversion of hypoxanthine and guanine to their respective mononucleotides. Hypoxanthine 126-138 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-52 1445289-2 1992 For instance, mutants resistant to azahypoxanthine showed a loss of the HPRT enzyme (hypoxanthine phosphoribosyl transferase), whereas gain of the same enzyme was accomplished by blocking de novo purine biosynthesis with aminopterin, while supplying hypoxanthine and thymine (HAT selection). Hypoxanthine 38-50 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 72-76 35194983-6 2022 Additionally, 32 genes were identified as under positive selection in cetaceans, including key purine salvage enzymes (i.e., HPRT1), suggesting improved re-utilization of non-recyclable purines avoid hypoxanthine accumulation and reduce oxidative stress. Hypoxanthine 200-212 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 125-130 1712904-1 1991 The enzyme hypoxanthine phosphoribosyltransferase (HPRT) catalyzes the metabolic salvage of the purine bases hypoxanthine and guanine. Hypoxanthine 11-23 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 51-55 2071157-1 1991 Hypoxanthine--guanine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes the conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate. Hypoxanthine 115-127 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-47 2071157-1 1991 Hypoxanthine--guanine phosphoribosyltransferase (HPRT) is a purine salvage enzyme that catalyzes the conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate. Hypoxanthine 115-127 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 49-53 1886405-0 1991 The pathogenesis of the Lesch-Nyhan syndrome: ATP use is positively related to hypoxanthine supply to hypoxanthine guanine phosphoribosyltransferase. Hypoxanthine 79-91 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 102-148 1886405-3 1991 We have shown that ATP turnover continuously supplies hypoxanthine for recycling by the enzyme HPRT and that this supply increases curvilinearly with increasing ATP turnover. Hypoxanthine 54-66 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 95-99 9092930-4 1997 A HAT (hypoxanthine, aminopterin, thymidine) sensitivity test allowed us to infer that Hprt- clones, selected as 6-thioguanine-resistant clones, possessed mutations at the Hprt locus after 532 nm Nd:YAG laser irradiation. Hypoxanthine 7-19 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 87-91 1583701-3 1992 The proportion of hypoxanthine converted to IMP by hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) is markedly greater than that degraded to xanthine and uric acid by xanthine oxidase (EC 1.3.2.3). Hypoxanthine 18-30 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 51-97 1731938-5 1992 The steady-state kinetic mechanism for the schistosomal HGPRTase has been determined by including both hypoxanthine and guanine in the forward and reverse reactions under identical conditions. Hypoxanthine 103-115 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 56-64 1850982-8 1991 The purine phosphoribosyltransferase activity with adenine was highest, about tenfold the HGPRTase activity with hypoxanthine and fivefold that with guanine. Hypoxanthine 113-125 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 90-98 1850982-10 1991 The Km values of APRTase for adenine and PP-Rib-P are 2 and 30 microM, respectively, and the Km values of HGPRTase for hypoxanthine, guanine and PP-Rib-P are less than 1, less than 1 and 15 microM, respectively. Hypoxanthine 119-131 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 106-114 2567208-3 1989 Allopurinol was also given to inhibit xanthine oxidase activity to decrease uric acid excretion and to elevate the serum concentration of hypoxanthine, which should competitively inhibit the activity of hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), the salvage enzyme of guanylate synthesis. Hypoxanthine 138-150 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 203-249 2543233-1 1989 Hypoxanthine-guanine phosphoribosyltransferase (HPRT) catalyzes the conversion of hypoxanthine and guanine to IMP and GMP, respectively, in the presence of 5-phosphoribosyl-1-pyrophosphate. Hypoxanthine 82-94 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 2543233-1 1989 Hypoxanthine-guanine phosphoribosyltransferase (HPRT) catalyzes the conversion of hypoxanthine and guanine to IMP and GMP, respectively, in the presence of 5-phosphoribosyl-1-pyrophosphate. Hypoxanthine 82-94 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-52 3221837-6 1988 Some of the latter were probably HPRT- nonsense mutants because they were very stringent (had less than 2% of wild-type [3H]hypoxanthine incorporation and HPRT enzyme activity), and did not complement genetically. Hypoxanthine 124-136 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 33-37 2437821-2 1987 Inosinic acid formed from the enzyme-catalyzed reaction of hypoxanthine and PP-ribose-P using partially purified hypoxanthine-guanine phosphoribosyltransferase is measured after chromatography on an ion-exchange column (Partisil 10 SAX). Hypoxanthine 59-71 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 113-159 3148065-3 1988 Accumulation of the substrate, hypoxanthine, of the missing hypoxanthine guanine phosphoribosyltransferase (HPRT) enzyme, is more marked in urine and in CSF than in plasma. Hypoxanthine 31-43 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 60-106 3148065-3 1988 Accumulation of the substrate, hypoxanthine, of the missing hypoxanthine guanine phosphoribosyltransferase (HPRT) enzyme, is more marked in urine and in CSF than in plasma. Hypoxanthine 31-43 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 108-112 3148065-5 1988 The function of HPRT seems to be the recycling of hypoxanthine which is released from tissues in increasing quantities as energy use, ATP "turnover", in the tissue increases. Hypoxanthine 50-62 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 16-20 3435682-1 1987 WI-L2 B lymphoblasts deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT) excreted amounts of hypoxanthine two to three times larger than CEM T lymphoblasts deficient in HGPRT, despite similar growth rates. Hypoxanthine 34-46 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 82-87 3435682-1 1987 WI-L2 B lymphoblasts deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT) excreted amounts of hypoxanthine two to three times larger than CEM T lymphoblasts deficient in HGPRT, despite similar growth rates. Hypoxanthine 34-46 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 185-190 3941107-6 1986 The activity staining pattern remains the same whether hypoxanthine or guanine is used as the substrate, further supporting the existence of a single protein, hypoxanthine-guanine phosphoribosyltransferase. Hypoxanthine 55-67 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 159-205 3456059-4 1986 Three mutant clones were established, which have remained hypoxanthine phosphoribosyltransferase (HPRT) deficient for a period of 7 months, as shown by indirect measurements with the use of autoradiography and scintillation counting of cells exposed to [3H]hypoxanthine. Hypoxanthine 58-70 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 98-102 6327016-8 1984 For both liver and hepatoma HGPRT, the reciprocal plots for hypoxanthine and guanine yielded the same Km of 3 microM. Hypoxanthine 60-72 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 28-33 3939543-1 1985 The low activity of the human variant HPRT Cape Town is associated with substrate inhibition by hypoxanthine and guanine in vitro. Hypoxanthine 96-108 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 38-42 3939543-5 1985 These results indicate that if substrate inhibition is responsible for the low intracellular activity of HPRT Cape Town, the concentration of either hypoxanthine or guanine in the vicinity of the active site of the enzyme must be greater than the Ki(app) for these substrates, 118 and 28 mumol L(-1) respectively. Hypoxanthine 149-161 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 105-109 6614674-2 1983 Activity of hypoxanthine guanine phosphoribosyl transferase (HGPRT) was studied in an intact fibroblast system in which the pattern of incorporation of isotope of 14C-labeled hypoxanthine into its purine products was assessed. Hypoxanthine 12-24 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 61-66 6706936-7 1984 This amino acid substitution lies within the putative hypoxanthine-binding site of human hypoxanthine-guanine phosphoribosyltransferase possibly explaining its selective effect on intrinsic enzyme activity and binding of hypoxanthine. Hypoxanthine 54-66 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 89-135 6526001-1 1984 Correlation of HPRT activity with hypoxanthine utilization and growth in selection media. Hypoxanthine 34-46 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 15-19 6526001-2 1984 In the present study, hypoxanthine phosphoribosyltransferase (HPRT) has been investigated in fibroblasts of 19 patients from 16 different families with HPRT deficiency, concerning activity, incorporation of 14C-hypoxanthine, and growth in 8-azaguanine and HAT (hypoxanthine, azaserine, thymidine containing) selection media. Hypoxanthine 22-34 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 62-66 6204922-2 1984 The affected males were found to have markedly reduced levels of erythrocytic hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity; these were 5-12% with hypoxanthine and 0.5-3% with guanine as compared to controls. Hypoxanthine 78-90 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 126-131 6852123-1 1983 A significant and reproducible enhancement of purine nucleotide synthesis from hypoxanthine occurs in HAT medium, when communication-competent hypoxanthine-guanine phosphoribosyltransferase (HGPRT+) cells are co-cultured with communication-competent (HGPRT-) LN cells. Hypoxanthine 79-91 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 143-189 6852123-1 1983 A significant and reproducible enhancement of purine nucleotide synthesis from hypoxanthine occurs in HAT medium, when communication-competent hypoxanthine-guanine phosphoribosyltransferase (HGPRT+) cells are co-cultured with communication-competent (HGPRT-) LN cells. Hypoxanthine 79-91 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 191-196 6852123-1 1983 A significant and reproducible enhancement of purine nucleotide synthesis from hypoxanthine occurs in HAT medium, when communication-competent hypoxanthine-guanine phosphoribosyltransferase (HGPRT+) cells are co-cultured with communication-competent (HGPRT-) LN cells. Hypoxanthine 79-91 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 251-256 6572373-3 1983 This enzyme variant, which is called HPRTLondon, is characterized by a decreased concentration of HPRT protein in erythrocytes and lymphoblasts, a normal Vmax, a 5-fold increased Km for hypoxanthine, a normal isoelectric point, and an apparently smaller subunit molecular weight. Hypoxanthine 186-198 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 37-41 6300847-3 1983 Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium. Hypoxanthine 191-203 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 16-20 7228032-1 1981 Hypoxanthine incorporation was studied in growing HPRT mutant cells by preincubating them with extracts from normal cells, HPRT mutant cells, and extracts of their lyophilized cell sediment. Hypoxanthine 0-12 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 50-54 7228032-1 1981 Hypoxanthine incorporation was studied in growing HPRT mutant cells by preincubating them with extracts from normal cells, HPRT mutant cells, and extracts of their lyophilized cell sediment. Hypoxanthine 0-12 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 123-127 7394700-5 1980 We also present evidence which shows that such mutants arise through a mutation that specifically alters the HPRT molecule so that the enzyme no longer recognizes 8-azaguanine as a substrate, while remaining catalytically functional with hypoxanthine and 6-thioguanine. Hypoxanthine 238-250 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 109-113 435498-12 1979 In vivo, HPRT+ cells, in contrast to HPRT- cells, may be operating purine de novo synthesis at a reduced rate because of their ability to reutilise hypoxanthine. Hypoxanthine 148-160 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 9-13 762946-2 1979 In comparison to normal HGPRTase the mutant enzyme from erythrocytes was found to have an elevated apparent Km-value for hypoxanthine and guanine and a lower Km-value for PRPP. Hypoxanthine 121-133 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 24-32 437792-1 1979 The uptake of adenine and hypoxanthine in HGPRT-deficient and normal human erythrocytes was measured using a rapid filtering centrifugation technique. Hypoxanthine 26-38 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 42-47 437792-3 1979 The transport of hypoxanthine into HGPRT-deficient erythrocytes differs from that into normal cells with respect to a higher accumulation capacity, to lower initial velocities and to the kinetic properties of the translocator. Hypoxanthine 17-29 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 35-40 7402756-9 1980 The Km of HGPRTase for hypoxanthine in cultivated cells and human tissues were similar to that in erythrocytes and leukocytes. Hypoxanthine 23-35 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 10-18 6258915-5 1980 In HGPRT-containing cells, salvage IMP synthesis from preformed and exogenously supplied hypoxanthine is the main source for IMP production. Hypoxanthine 89-101 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 3-8 477861-2 1979 PRPP is reacted with excess hypoxanthine in the presence of hypoxanthine-guanine phosphoribosyltransferase. Hypoxanthine 28-40 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 60-106 435498-4 1979 Fetal calf serum, which was used to supplement the assay and cell growth medium, was found to contain sufficient quantities of the purine base hypoxanthine to inhibit purine de novo synthesis in HPRT+ cells. Hypoxanthine 143-155 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 195-199 891652-0 1977 Transport of hypoxanthine by human diploid skin fibroblasts deficient in hypoxanthine-guanine phosphoribosyltransferase. Hypoxanthine 13-25 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 73-119 862666-0 1977 Hypoxanthine transport in normal and hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficient diploid human lymphoblasts. Hypoxanthine 0-12 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 37-83 862666-0 1977 Hypoxanthine transport in normal and hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficient diploid human lymphoblasts. Hypoxanthine 0-12 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 85-90 305334-2 1977 Hypoxanthine-guanine phosphoribosyltransferase (HGPRT, EC 2.4.2.8) catalyses the conversion of hypoxanthine and guinine into their respective nucleotides. Hypoxanthine 95-107 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 305334-2 1977 Hypoxanthine-guanine phosphoribosyltransferase (HGPRT, EC 2.4.2.8) catalyses the conversion of hypoxanthine and guinine into their respective nucleotides. Hypoxanthine 95-107 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-53 993266-6 1976 The utilization of [3H]hypoxanthine by communicating mixtures of HPRT+ and HPRT- human cells is not significantly different than in the normal cells alone. Hypoxanthine 23-35 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 65-69 993266-6 1976 The utilization of [3H]hypoxanthine by communicating mixtures of HPRT+ and HPRT- human cells is not significantly different than in the normal cells alone. Hypoxanthine 23-35 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 75-79 932196-2 1976 In the present study, the conversion of hypoxanthine to phosphorylated products was measured in intact skin fibroblasts and in cell extracts from seven patients with mutant hypoxanthine-guanine phosphoribosyltransferase (HPRT) and six control subjects. Hypoxanthine 40-52 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 173-219 932196-2 1976 In the present study, the conversion of hypoxanthine to phosphorylated products was measured in intact skin fibroblasts and in cell extracts from seven patients with mutant hypoxanthine-guanine phosphoribosyltransferase (HPRT) and six control subjects. Hypoxanthine 40-52 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 221-225 4426333-0 1974 Incorporation of hypoxanthine by PHA-stimulated HPRT-deficient lymphocytes. Hypoxanthine 17-29 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-52 4753213-0 1973 Transport of hypoxanthine in fibroblasts with normal and mutant hypoxanthine-guanine phosphoribosyltransferase. Hypoxanthine 13-25 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 64-110 30895693-5 2019 The Hprt gene of the endogenous X chromosome was inactivated by CRISPR/Cas9 technology thus allowing the exploitation of the hypoxanthine-aminopterin-thymidine selection system to introduce a normal donor X chromosome by microcell-mediated chromosome transfer. Hypoxanthine 125-137 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 4-8 5763653-0 1969 Hypoxanthine-guanine phosphoribosyltransferase: further evidence for the identity of the binding sites for hypoxanthine and guanine. Hypoxanthine 107-119 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 26968365-1 2016 Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (EC 2.4.2.8) reversibly catalyzes the transfer of the 5-phophoribosyl group from 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP) to hypoxanthine or guanine to form inosine monophosphate (IMP) or guanosine monophosphate (GMP) in the purine salvage pathway. Hypoxanthine 185-197 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 48-53 29306799-8 2018 Hypoxanthine, guanine, inosine and guanosine are salvaged to IMP and GMP by hypoxanthine/guanine phosphoribosyltransferase (EC 2.4.2.8) and inosine/guanosine kinase (EC 2.7.1.73). Hypoxanthine 0-12 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 76-122 29416770-7 2018 The HPRT1 mutation dramaticly decreased the production of [13C5,15N4]-IMP from [13C5,15N4]-hypoxanthine (HX), showed a loss-of-funciton mechanism. Hypoxanthine 91-103 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 4-9 26067813-2 2016 HGprt has two functions; it recycles hypoxanthine and guanine. Hypoxanthine 37-49 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-5 26368337-5 2015 The crystal structures of HPEPG and HPEPHx (where Hx=hypoxanthine) in complex with human HGPRT were obtained, showing specific interactions with active site residues. Hypoxanthine 53-65 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 89-94 26902413-2 2015 Human HGPRT follows a steady-state ordered mechanism, wherein PRPP binding precedes the binding of hypoxanthine/guanine and release of product IMP/GMP is the rate limiting step. Hypoxanthine 99-111 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 6-11 22157001-5 2012 In the current studies 44 HPRT1 mutations associated with a wide spectrum of clinical phenotypes were reconstructed by site-directed mutagenesis, the mutant enzymes were expressed in vitro and purified, and their kinetic properties were examined toward their substrates hypoxanthine, guanine, and phosphoribosylpyrophosphate. Hypoxanthine 270-282 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 26-31 21904906-4 2012 However, when the HPRT activity was measured at low substrate concentrations [phosphoribosyl pyrophosphate (PRPP) and hypoxanthine] and compared with usual assay conditions, the HPRT activity at lower PRPP was less in the propositus, suggesting altered enzyme kinetics. Hypoxanthine 118-130 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 18-22 21904906-4 2012 However, when the HPRT activity was measured at low substrate concentrations [phosphoribosyl pyrophosphate (PRPP) and hypoxanthine] and compared with usual assay conditions, the HPRT activity at lower PRPP was less in the propositus, suggesting altered enzyme kinetics. Hypoxanthine 118-130 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 178-182 21486037-5 2011 Human hypoxanthine guanine phosphoribosyltransferase (hHGPRT) catalyzes the phosphoribosylation of guanine and hypoxanthine, while the Plasmodium falciparum HGPRT (PfHGPRT) acts on xanthine as well. Hypoxanthine 6-18 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 54-60 21486037-5 2011 Human hypoxanthine guanine phosphoribosyltransferase (hHGPRT) catalyzes the phosphoribosylation of guanine and hypoxanthine, while the Plasmodium falciparum HGPRT (PfHGPRT) acts on xanthine as well. Hypoxanthine 6-18 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 55-60 21054786-1 2010 Human hypoxanthine-guanine phosphoribosyltransferase (HPRT) (EC 2.4.2.8) catalyzes the conversion of hypoxanthine and guanine to their respective nucleoside monophosphates. Hypoxanthine 6-18 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 54-58 21054786-8 2010 However, the catalytic efficiency (k(cat)/K(m)) of PRTFDC1 towards hypoxanthine and guanine was only 0.26% and 0.09%, respectively, of that of HPRT. Hypoxanthine 67-79 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 143-147 18710792-2 2008 The enzyme HPRT is part of the savage pathway of purine biosynthesis and catalyzes the conversion of hypoxanthine and guanine to their respective nucleotides, IMP and GMP. Hypoxanthine 101-113 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 11-15