PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 17848616-4 2007 We show that PF4/heparin macromolecular assembly occurs through colloidal interactions, wherein heparin facilitates the growth of complexes through charge neutralization. Heparin 17-24 platelet factor 4 Mus musculus 13-16 20959601-3 2011 These antibodies probably result from bacterial infections, as (1) PF4 bound charge-dependently to various bacteria, (2) human heparin-induced anti-PF4/heparin antibodies cross-reacted with PF4-coated Staphylococcus aureus and Escherichia coli, and (3) mice developed anti-PF4/heparin antibodies during polymicrobial sepsis without heparin application. Heparin 127-134 platelet factor 4 Mus musculus 148-151 19245419-1 2009 SUMMARY BACKGROUND: Heparin-induced thrombocytopenia (HIT) is a life-threatening thrombotic illness caused by drug-dependent antibodies recognizing complexes of platelet factor 4 (PF4) and heparin. Heparin 20-27 platelet factor 4 Mus musculus 180-183 20961394-9 2011 As evidenced by the reduction of the activated partial thromboplastin time, CXCL4L1 showed a tendency towards less heparin-neutralizing activity than CXCL4 (IC(50) 2.45 vs 0.98 mug mL(-1)). Heparin 115-122 platelet factor 4 Mus musculus 76-81 20724543-0 2010 Monocyte-bound PF4 in the pathogenesis of heparin-induced thrombocytopenia. Heparin 42-49 platelet factor 4 Mus musculus 15-18 17848616-4 2007 We show that PF4/heparin macromolecular assembly occurs through colloidal interactions, wherein heparin facilitates the growth of complexes through charge neutralization. Heparin 96-103 platelet factor 4 Mus musculus 13-16 17848616-5 2007 The size of PF4/heparin macromolecules is governed by the molar ratios of the reactants. Heparin 16-23 platelet factor 4 Mus musculus 12-15 17848616-6 2007 Maximal complex size occurs at molar ratios of PF4/heparin at which surface charge is neutral. Heparin 51-58 platelet factor 4 Mus musculus 47-50 17848616-8 2007 These studies suggest that the clinical heterogeneity in the HIT immune response may be due in part to requirements for specific biophysical parameters of the PF4/heparin complexes that occur in settings of intense platelet activation and PF4 release. Heparin 163-170 platelet factor 4 Mus musculus 159-162 17848616-8 2007 These studies suggest that the clinical heterogeneity in the HIT immune response may be due in part to requirements for specific biophysical parameters of the PF4/heparin complexes that occur in settings of intense platelet activation and PF4 release. Heparin 163-170 platelet factor 4 Mus musculus 239-242 15845897-1 2005 Heparin-induced thrombocytopenia (HIT) is a life-threatening, thrombotic disorder associated with development of anti-platelet factor 4 (anti-PF4)/heparin autoantibodies. Heparin 0-7 platelet factor 4 Mus musculus 142-145 15845897-5 2005 Mice injected with mPF4/heparin, but not mPF4 or heparin alone, developed heparin-dependent autoantibodies that shared serologic and functional characteristics of human HIT antibodies, including preferential binding to mPF4/heparin complexes and causing heparin- and FcRgammaIIA-dependent platelet activation. Heparin 24-31 platelet factor 4 Mus musculus 19-23 11830470-1 2002 Heparin-induced thrombocytopenia/thrombosis (HIT/T) is a common complication of heparin therapy that is caused by antibodies to platelet factor 4 (PF4) complexed with heparin. Heparin 0-7 platelet factor 4 Mus musculus 147-150 14764524-2 2004 PF4 binds to heparin with high affinity, but its in vivo biologic role has not been defined. Heparin 13-20 platelet factor 4 Mus musculus 0-3 14764524-6 2004 The thrombotic defect in the mPF4(+/-) and mPF4(-/-) animals was particularly sensitive to infusions of the negatively charged anticoagulant heparin. Heparin 141-148 platelet factor 4 Mus musculus 29-33 14764524-6 2004 The thrombotic defect in the mPF4(+/-) and mPF4(-/-) animals was particularly sensitive to infusions of the negatively charged anticoagulant heparin. Heparin 141-148 platelet factor 4 Mus musculus 43-47 11830470-1 2002 Heparin-induced thrombocytopenia/thrombosis (HIT/T) is a common complication of heparin therapy that is caused by antibodies to platelet factor 4 (PF4) complexed with heparin. Heparin 80-87 platelet factor 4 Mus musculus 147-150 11830470-1 2002 Heparin-induced thrombocytopenia/thrombosis (HIT/T) is a common complication of heparin therapy that is caused by antibodies to platelet factor 4 (PF4) complexed with heparin. Heparin 167-174 platelet factor 4 Mus musculus 147-150 25960020-5 2015 Complexes of PF4/low-molecular-weight heparin and complexes composed of heparin and murine PF4, protamine or lysozyme are internalized similarly, suggesting a common endocytic pathway. Heparin 38-45 platelet factor 4 Mus musculus 13-16 9930558-1 1999 Heparin-induced thrombocytopenia (HIT) occurs in 1% to 3% of patients receiving heparin and results from the development of antibodies that recognize heparin-platelet factor 4 (H-PF4) complexes that form on the surface of activated platelets and on the vascular endothelium. Heparin 0-7 platelet factor 4 Mus musculus 179-182 9930558-1 1999 Heparin-induced thrombocytopenia (HIT) occurs in 1% to 3% of patients receiving heparin and results from the development of antibodies that recognize heparin-platelet factor 4 (H-PF4) complexes that form on the surface of activated platelets and on the vascular endothelium. Heparin 80-87 platelet factor 4 Mus musculus 179-182 9871804-7 1998 Recently, it has been shown that a peptide of PF4, 34-58 which does not contain the site of heparin binding, is able to inhibit the growth of hematopoietic progenitors in vitro, providing evidence for a model of heparin dependent and independent pathways of PF4 action on hematopoiesis. Heparin 92-99 platelet factor 4 Mus musculus 46-49 3409328-2 1988 The present studies show that PF4 prepared from normal mouse or human serum by absorption to heparin-agarose and elution between 0.5 and 1.5 M NaCl is also active in this respect. Heparin 93-100 platelet factor 4 Mus musculus 30-33 6219115-5 1983 PF4-mediated responses were blocked by treating the PF4-adsorbed substratum with heparin (but not chondroitin sulfate), or alternatively the cells with Flavobacter heparinum heparinase (but not chondroitinase ABC). Heparin 81-88 platelet factor 4 Mus musculus 0-3 6219115-5 1983 PF4-mediated responses were blocked by treating the PF4-adsorbed substratum with heparin (but not chondroitin sulfate), or alternatively the cells with Flavobacter heparinum heparinase (but not chondroitinase ABC). Heparin 81-88 platelet factor 4 Mus musculus 52-55 31471526-6 2019 Adoptively transferred Treg cells prevented spontaneous production of PF4/heparin-specific Abs in Foxp3-deficient mice and inhibited PF4/heparin complex-induced production of PF4/heparin-specific IgGs in wild-type mice. Heparin 137-144 platelet factor 4 Mus musculus 133-136 31471526-6 2019 Adoptively transferred Treg cells prevented spontaneous production of PF4/heparin-specific Abs in Foxp3-deficient mice and inhibited PF4/heparin complex-induced production of PF4/heparin-specific IgGs in wild-type mice. Heparin 137-144 platelet factor 4 Mus musculus 133-136 31471526-9 2019 Moreover, bone marrow chimeric mice with CD4 T cell-specific deletion of IL-10 increased PF4/heparin-specific IgG production upon PF4/heparin complex challenge. Heparin 93-100 platelet factor 4 Mus musculus 89-92 31471526-9 2019 Moreover, bone marrow chimeric mice with CD4 T cell-specific deletion of IL-10 increased PF4/heparin-specific IgG production upon PF4/heparin complex challenge. Heparin 93-100 platelet factor 4 Mus musculus 130-133 31471526-9 2019 Moreover, bone marrow chimeric mice with CD4 T cell-specific deletion of IL-10 increased PF4/heparin-specific IgG production upon PF4/heparin complex challenge. Heparin 134-141 platelet factor 4 Mus musculus 89-92 26467272-5 2016 In addition, PF4/heparin-specific IgM B cells were not only found in murine spleen, but also in peritoneum and bone marrow upon in vitro stimulation. Heparin 17-24 platelet factor 4 Mus musculus 13-16 26467272-8 2016 In conclusion, the anti-PF4/heparin IgM response is a potential innate immune reaction driven by a B cell population distinct from MZ B cells. Heparin 28-35 platelet factor 4 Mus musculus 24-27 9558385-9 1998 In the presence of heparin, PF-4 abolished totally the stimulatory effect of heparin. Heparin 19-26 platelet factor 4 Mus musculus 28-32 9558385-9 1998 In the presence of heparin, PF-4 abolished totally the stimulatory effect of heparin. Heparin 77-84 platelet factor 4 Mus musculus 28-32 9558385-10 1998 Furthermore, PF-4 complexed to FGF-2 and inhibited endogenous or heparin-induced FGF-2 dimerization. Heparin 65-72 platelet factor 4 Mus musculus 13-17 8562407-7 1996 Moreover, such an inhibitory activity of PF4-related peptides is abrogated by heparin (5 IU/dish). Heparin 78-85 platelet factor 4 Mus musculus 41-44 35498738-2 2022 As a potential therapeutic agent in the treatment of sepsis, 2-O, 3-O desulfated heparin (ODSH) reduces histones and platelet factor 4 (PF4) in mouse sepsis models. Heparin 81-88 platelet factor 4 Mus musculus 136-139 32077913-1 2020 Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder mediated by complexes between platelet factor 4 (PF4) and heparin or other polyanions, but the risk of thrombosis extends beyond exposure to heparin implicating other PF4 partners. Heparin 0-7 platelet factor 4 Mus musculus 116-119 32077913-1 2020 Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder mediated by complexes between platelet factor 4 (PF4) and heparin or other polyanions, but the risk of thrombosis extends beyond exposure to heparin implicating other PF4 partners. Heparin 0-7 platelet factor 4 Mus musculus 234-237 32077913-1 2020 Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder mediated by complexes between platelet factor 4 (PF4) and heparin or other polyanions, but the risk of thrombosis extends beyond exposure to heparin implicating other PF4 partners. Heparin 208-215 platelet factor 4 Mus musculus 116-119 31471526-6 2019 Adoptively transferred Treg cells prevented spontaneous production of PF4/heparin-specific Abs in Foxp3-deficient mice and inhibited PF4/heparin complex-induced production of PF4/heparin-specific IgGs in wild-type mice. Heparin 74-81 platelet factor 4 Mus musculus 70-73 29914979-1 2018 Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes. Heparin 0-7 platelet factor 4 Mus musculus 113-116 24816371-0 2014 New insights in heparin-induced thrombocytopenia by the use of fluid-phase assays to detect specifically platelet factor 4/heparin complex antibodies and antibody-secreting cells. Heparin 16-23 platelet factor 4 Mus musculus 105-122 25595736-3 2015 Here, we showed that induction of PF4/heparin-specific antibodies by PF4/heparin complexes was markedly impaired in mice depleted of CD4 T cells by anti-CD4 antibodies. Heparin 38-45 platelet factor 4 Mus musculus 34-37 25595736-3 2015 Here, we showed that induction of PF4/heparin-specific antibodies by PF4/heparin complexes was markedly impaired in mice depleted of CD4 T cells by anti-CD4 antibodies. Heparin 38-45 platelet factor 4 Mus musculus 69-72 25595736-4 2015 Furthermore, Rag1-deficient recipient mice produced PF4/heparin-specific antibodies upon PF4/heparin challenge when reconstituted with a mixture of wild-type splenic B cells and splenocytes from B-cell-deficient (muMT) mice but not splenocytes from T- and B-cell-deficient (Rag1 knockout) mice. Heparin 56-63 platelet factor 4 Mus musculus 89-92 25595736-4 2015 Furthermore, Rag1-deficient recipient mice produced PF4/heparin-specific antibodies upon PF4/heparin challenge when reconstituted with a mixture of wild-type splenic B cells and splenocytes from B-cell-deficient (muMT) mice but not splenocytes from T- and B-cell-deficient (Rag1 knockout) mice. Heparin 93-100 platelet factor 4 Mus musculus 52-55 25595736-5 2015 Lastly, mice with B cells lacking CD40, a B-cell costimulatory molecule that helps T-cell-dependent B-cell responses, displayed a marked reduction of PF4/heparin-specific antibody production following PF4/heparin challenge. Heparin 154-161 platelet factor 4 Mus musculus 150-153 24816371-2 2014 These Ab are directed against neoepitopes of the PF4 tetramer, which are induced by the complex formation with heparin. Heparin 111-118 platelet factor 4 Mus musculus 49-52 24816371-6 2014 This fluid-phase approach applied to the detection of specific murine PF4/heparin Ab-secreting cells (ASC) identified the spleen as the main lymphatic organ that contributes to the PF4/heparin Ab response in mice. Heparin 74-81 platelet factor 4 Mus musculus 181-184 24816371-1 2014 INTRODUCTION: The key feature of heparin-induced thrombocytopenia (HIT) is the production of antibodies (Ab) against the platelet factor 4 (PF4)/heparin complex. Heparin 33-40 platelet factor 4 Mus musculus 121-138 24816371-1 2014 INTRODUCTION: The key feature of heparin-induced thrombocytopenia (HIT) is the production of antibodies (Ab) against the platelet factor 4 (PF4)/heparin complex. Heparin 33-40 platelet factor 4 Mus musculus 140-143 21659541-1 2011 Platelet factor 4 (PF4) and heparin (H) form PF4/H complexes, the target of the immune reaction in heparin-induced thrombocytopenia (HIT). Heparin 28-35 platelet factor 4 Mus musculus 45-48 21659541-1 2011 Platelet factor 4 (PF4) and heparin (H) form PF4/H complexes, the target of the immune reaction in heparin-induced thrombocytopenia (HIT). Heparin 99-106 platelet factor 4 Mus musculus 0-17 21659541-1 2011 Platelet factor 4 (PF4) and heparin (H) form PF4/H complexes, the target of the immune reaction in heparin-induced thrombocytopenia (HIT). Heparin 99-106 platelet factor 4 Mus musculus 19-22 21659541-1 2011 Platelet factor 4 (PF4) and heparin (H) form PF4/H complexes, the target of the immune reaction in heparin-induced thrombocytopenia (HIT). Heparin 99-106 platelet factor 4 Mus musculus 45-48 21659541-2 2011 HIT seems to be a secondary immune response as anti-PF4/H-IgG antibodies occur as early as day 4 of heparin treatment. Heparin 100-107 platelet factor 4 Mus musculus 52-55