PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 2465300-4 1989 Under conditions where heparin potentiated aFGF-induced neurite outgrowth, we observed that heparin increased the biological half-life of aFGF from 7 to 39 hr. Heparin 92-99 fibroblast growth factor 1 Rattus norvegicus 138-142 2791981-4 1989 However, in the Ob cultures, bFGF was intrinsically 10-fold more stimulatory than aFGF, whereas heparin enhanced the mitotic response only to aFGF. Heparin 96-103 fibroblast growth factor 1 Rattus norvegicus 142-146 2465300-1 1989 The mechanism(s) by which heparin influences the biological activities of acidic and basic fibroblast growth factors (aFGF and bFGF) is not completely understood. Heparin 26-33 fibroblast growth factor 1 Rattus norvegicus 118-122 2465300-2 1989 One mechanism by which heparin could alter the biological activities of aFGF and bFGF is by altering their biological half-lives. Heparin 23-30 fibroblast growth factor 1 Rattus norvegicus 72-76 2465300-6 1989 If aFGF activity was maintained for greater than 25 hr by periodic readdition of factor, heparin no longer potentiated aFGF-induced neurite outgrowth. Heparin 89-96 fibroblast growth factor 1 Rattus norvegicus 3-7 2465300-3 1989 We investigated the possibility that heparin potentiates aFGF-induced neurite outgrowth from PC12 cells by prolonging its biological half-life. Heparin 37-44 fibroblast growth factor 1 Rattus norvegicus 57-61 2465300-4 1989 Under conditions where heparin potentiated aFGF-induced neurite outgrowth, we observed that heparin increased the biological half-life of aFGF from 7 to 39 hr. Heparin 23-30 fibroblast growth factor 1 Rattus norvegicus 43-47 2465300-7 1989 These observations strongly suggest that heparin potentiates the activity of aFGF by prolonging its biological half-life. Heparin 41-48 fibroblast growth factor 1 Rattus norvegicus 77-81 2465300-4 1989 Under conditions where heparin potentiated aFGF-induced neurite outgrowth, we observed that heparin increased the biological half-life of aFGF from 7 to 39 hr. Heparin 23-30 fibroblast growth factor 1 Rattus norvegicus 138-142 2465300-10 1989 These observations suggest that heparin regulates the activity of aFGF by regulating its proteolytic degradation, thereby regulating its biological half-life. Heparin 32-39 fibroblast growth factor 1 Rattus norvegicus 66-70 2465300-4 1989 Under conditions where heparin potentiated aFGF-induced neurite outgrowth, we observed that heparin increased the biological half-life of aFGF from 7 to 39 hr. Heparin 92-99 fibroblast growth factor 1 Rattus norvegicus 43-47 3032991-4 1987 While the neurotropic activities of aFGF and NGF are potentiated by heparin, that of bFGF is both partially inhibited or stimulated, depending upon the concentration of bFGF. Heparin 68-75 fibroblast growth factor 1 Rattus norvegicus 36-40 3272188-3 1988 Heparin strongly potentiates aFGF-dependent neuritic outgrowth but not aFGF-dependent proliferation. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 29-33 2967302-2 1988 Recent studies have shown that heparin, a GAG found in mast cells, potentiates the ability of acidic fibroblast growth factor (aFGF) to induce neurite outgrowth in pheochromocytoma (PC12) cells. Heparin 31-38 fibroblast growth factor 1 Rattus norvegicus 94-125 2967302-2 1988 Recent studies have shown that heparin, a GAG found in mast cells, potentiates the ability of acidic fibroblast growth factor (aFGF) to induce neurite outgrowth in pheochromocytoma (PC12) cells. Heparin 31-38 fibroblast growth factor 1 Rattus norvegicus 127-131 2967302-5 1988 Heparin potentiated aFGF-induced neurite outgrowth in a concentration-dependent fashion; potentiation increased with increasing heparin concentrations of 0.01-100 micrograms/ml. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 20-24 2967302-5 1988 Heparin potentiated aFGF-induced neurite outgrowth in a concentration-dependent fashion; potentiation increased with increasing heparin concentrations of 0.01-100 micrograms/ml. Heparin 128-135 fibroblast growth factor 1 Rattus norvegicus 20-24 2967302-7 1988 The maximally active concentration of heparin (100 micrograms/ml) increased the potency of aFGF 102-fold. Heparin 38-45 fibroblast growth factor 1 Rattus norvegicus 91-95 3068010-5 1988 Studies with primary rat osteoblast-like cells exposed either to mixed BDGFs, pure TGF-beta, or heparin-purified PDGF, aFGF, or bFGF from bovine bone have shown a general dose-dependent mitogenic effect. Heparin 96-103 fibroblast growth factor 1 Rattus norvegicus 119-123 2912214-2 1989 This FGF-like activity in the cerebrospinal fluid was bioassayed in two systems: depression of the feeding response of Hydra and DNA synthesis-stimulating activity in BALB/c 3T3 cells after fractionation on a heparin affinity column. Heparin 209-216 fibroblast growth factor 1 Rattus norvegicus 5-8 2906331-9 1988 The mitogenic activities of aFGF and bFGF were potentiated similarly by heparan sulfate and by heparin, with a maximum stimulation of about 100% at 100 micrograms/ml heparin. Heparin 95-102 fibroblast growth factor 1 Rattus norvegicus 28-32 2906331-9 1988 The mitogenic activities of aFGF and bFGF were potentiated similarly by heparan sulfate and by heparin, with a maximum stimulation of about 100% at 100 micrograms/ml heparin. Heparin 166-173 fibroblast growth factor 1 Rattus norvegicus 28-32 3032991-8 1987 Heparin blocked the binding of bFGF to the receptor but had only a small inhibitory effect on the binding of aFGF to the receptor. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 109-113 3032991-10 1987 The stimulatory effects of heparin on the neurotropic activity of aFGF, bFGF, and NGF may occur through a site not associated with the respective cellular receptor for the growth factors. Heparin 27-34 fibroblast growth factor 1 Rattus norvegicus 66-70 22758395-0 2012 Improving the cardio protective effect of aFGF in ischemic myocardium with ultrasound-mediated cavitation of heparin modified microbubbles: preliminary experiment. Heparin 109-116 fibroblast growth factor 1 Rattus norvegicus 42-46 32104478-4 2019 In the present study, we prepared sulfated chitooligosaccharides (SCOS), which have heparin-like properties, to improve the bioactivity of aFGF. Heparin 84-91 fibroblast growth factor 1 Rattus norvegicus 139-143 26947349-0 2016 Advanced Interfere Treatment of Diabetic Cardiomyopathy Rats by aFGF-Loaded Heparin-Modified Microbubbles and UTMD Technique. Heparin 76-83 fibroblast growth factor 1 Rattus norvegicus 64-68 26947349-1 2016 This study aims to investigate the preclinical performance and mechanism of a novel strategy of aFGF-loaded heparin-modified microbubbles (aFGF-HMB) combined with ultrasound-targeted microbubble destruction (UTMD) technique for diabetic cardiomyopathy (DCM) prevention. Heparin 108-115 fibroblast growth factor 1 Rattus norvegicus 96-100 26947349-1 2016 This study aims to investigate the preclinical performance and mechanism of a novel strategy of aFGF-loaded heparin-modified microbubbles (aFGF-HMB) combined with ultrasound-targeted microbubble destruction (UTMD) technique for diabetic cardiomyopathy (DCM) prevention. Heparin 108-115 fibroblast growth factor 1 Rattus norvegicus 139-143 22758395-2 2012 This paper was to investigate the feasibility of acidic fibroblast growth factor (aFGF) intravenous delivery to the ischemic myocardium of rats by ultrasonic microbubbles modified with heparin. Heparin 185-192 fibroblast growth factor 1 Rattus norvegicus 49-80 22758395-2 2012 This paper was to investigate the feasibility of acidic fibroblast growth factor (aFGF) intravenous delivery to the ischemic myocardium of rats by ultrasonic microbubbles modified with heparin. Heparin 185-192 fibroblast growth factor 1 Rattus norvegicus 82-86 21052646-1 2011 Heparin-immobilized microspheres were included in microdialysis sampling perfusion fluids under both in vitro and in vivo conditions to improve the recovery of different cytokines, acidic fibroblast growth factor, vascular endothelial growth factor, monocyte chemoattractant protein-1 (or CCL2), and regulation upon activation normal T cell express sequence (or CCL5). Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 181-212 11091973-5 2000 Addition of heparin to cultures decreased the most effective aFGF concentration by 100-fold, from 100 ng ml-1 to 1 ng ml-1. Heparin 12-19 fibroblast growth factor 1 Rattus norvegicus 61-65 12204897-7 2002 Total protein, cell number, and FGF-2 protein expression and mRNA of FGF-1, -2, and FGF receptor-2 detected by reverse transcriptase-polymerase chain reaction were decreased by heparin. Heparin 177-184 fibroblast growth factor 1 Rattus norvegicus 69-74 11451924-9 2001 Heparin as low as 0.01 mg/mL significantly downregulated expression of TGF-beta(1) and FGF-1-stimulated FGF-2 and FGFR-1. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 87-92 18540049-2 2008 Sucrose octasulfate (SOS), a chemical analogue of heparin, has been demonstrated to activate FGF signalling pathways. Heparin 50-57 fibroblast growth factor 1 Rattus norvegicus 93-96 14966081-0 2004 Heparin affects signaling pathways stimulated by fibroblast growth factor-1 and -2 in type II cells. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 49-82 14966081-2 2004 Heparin, a model for sulfated components of basement membrane matrices, is known to inhibit fibroblast growth factor (FGF)-2-stimulated DNA synthesis as well as gene expression of FGF-2 and its receptor in AT2 cells. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 118-121 14966081-5 2004 High-dose heparin reduced FGF-1- or FGF-2-stimulated phosphorylation of mitogen-activated protein kinase kinases (MEK1/2), p44/42 mitogen-activated protein kinases (MAPK/ERK1/2), stress-activated protein kinase/c-Jun NH(2)-terminal kinase, Akt/protein kinase B, and p90(RSK). Heparin 10-17 fibroblast growth factor 1 Rattus norvegicus 26-31 8576258-7 1996 Analysis of the PC12 cells after the addition of FGF1 plus heparin or FGF2 demonstrated a significant increase in the level of FGF1 expression with the same time course as the appearance of the neuritic extensions. Heparin 59-66 fibroblast growth factor 1 Rattus norvegicus 127-131 10938477-2 2000 The (99m)Tc-FGF-1 retained its representative molecular mass, heparin affinity, cellular binding to both low (Kd = 9.5 nM) and high (Kd = 125 pM) affinity sites, and mitogenic activity. Heparin 62-69 fibroblast growth factor 1 Rattus norvegicus 12-17 10938477-4 2000 Heparin significantly decreased (99m)Tc-FGF-1 liver uptake and increased urinary excretion. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 40-45 10419453-5 1999 The antithrombin-bound fraction of heparin was required to support the heparin-dependent stimulation of DNA synthesis of endothelial cells by FGF-1. Heparin 35-42 fibroblast growth factor 1 Rattus norvegicus 142-147 10419453-5 1999 The antithrombin-bound fraction of heparin was required to support the heparin-dependent stimulation of DNA synthesis of endothelial cells by FGF-1. Heparin 71-78 fibroblast growth factor 1 Rattus norvegicus 142-147 9279319-2 1997 Although heparin has no mitogenic potential of its own, it is an important aFGF cofactor in vitro and may also be capable of stimulating angiogenesis. Heparin 9-16 fibroblast growth factor 1 Rattus norvegicus 75-79 9279319-9 1997 CONCLUSIONS: The increased vascularization and mitogenic activity demonstrated by these respective studies suggest that angiogenesis is significantly accelerated by the administration of heparin alone and is accelerated to a greater extent by the administration of aFGF with or without heparin. Heparin 187-194 fibroblast growth factor 1 Rattus norvegicus 265-269 9731752-4 1998 FGF-1 in the culture medium produced a dose-dependent increase in the number and length of neurites produced by spiral ganglion neurons, a response that was enhanced by heparin. Heparin 169-176 fibroblast growth factor 1 Rattus norvegicus 0-5 8810636-9 1996 Acidic fibroblast growth factor, which also binds KGF receptors, in the presence of heparin mimicked the effect of KGF on branching. Heparin 84-91 fibroblast growth factor 1 Rattus norvegicus 0-31 17177827-3 1996 After 60 minutes of ischemia and at the onset of reperfusion, rats in the acidic fibroblast growth factor-treated group received 2.6 microg of acidic fibroblast growth factor/rat in 50 microl of phosphate-buffered saline solution containing 0.1% heparin (w/v) through the jugular vein, whereas the rats in the phosphate-buffered saline solution-treated group received the same vehicle without acidic fibroblast growth factor. Heparin 246-253 fibroblast growth factor 1 Rattus norvegicus 74-105 8698881-2 1996 We have shown that the heparin-binding growth factor FGF-1 is expressed by olfactory nerve ensheathing cells which surround fascicles of primary olfactory axons en route to the olfactory bulb. Heparin 23-30 fibroblast growth factor 1 Rattus norvegicus 53-58 7537691-0 1995 FGF-1 is a heparin-independent mitogen for rat hepatocytes. Heparin 11-18 fibroblast growth factor 1 Rattus norvegicus 0-5 7537691-1 1995 We have found that in the primary culture of rat hepatocytes, the potent mitogenic activity of native FGF-1 is independent of heparin. Heparin 126-133 fibroblast growth factor 1 Rattus norvegicus 102-107 7537691-4 1995 For both cell types, however, it is demonstrated that either endogenous or exogenous heparan sulfate/heparin moieties are essential for FGF-1 to establish receptor binding and mitogen action. Heparin 101-108 fibroblast growth factor 1 Rattus norvegicus 136-141 7528211-6 1994 268, 2984-2988) that heparin potentiates the mitogenic activity of acidic FGF (aFGF) but inhibits that of the keratinocyte growth factor (KGF) in cells that express the KGF receptor (KGFR). Heparin 21-28 fibroblast growth factor 1 Rattus norvegicus 67-77 7730327-3 1995 Here we show that a lower affinity of FGFR1 alpha for heparin parallels the lower affinity for FGF-1. Heparin 54-61 fibroblast growth factor 1 Rattus norvegicus 95-100 7528211-6 1994 268, 2984-2988) that heparin potentiates the mitogenic activity of acidic FGF (aFGF) but inhibits that of the keratinocyte growth factor (KGF) in cells that express the KGF receptor (KGFR). Heparin 21-28 fibroblast growth factor 1 Rattus norvegicus 79-83 7528211-8 1994 To gain an insight into the mechanism by which heparin modulates the biological activity of aFGF and KGF, we studied the effect of heparin and cell-associated heparan sulfates on the binding of these two growth factors to the KGFR. Heparin 47-54 fibroblast growth factor 1 Rattus norvegicus 92-96 7528211-11 1994 By contrast, similar concentrations of heparin enhanced the binding of aFGF to this receptor. Heparin 39-46 fibroblast growth factor 1 Rattus norvegicus 71-75 7528211-16 1994 Heparin restored the high affinity binding of aFGF to chlorate-treated cells and completely abolished the high affinity binding of KGF. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 46-50 1716635-3 1991 Immunoblotting of crude and heparin-bound embryo extracts revealed faint bands at the expected 17-18-kD and predominant bands at an apparent molecular mass of 26 to 28-kD (despite reducing conditions) using multiple specific antibodies for aFGF and bFGF. Heparin 28-35 fibroblast growth factor 1 Rattus norvegicus 240-244 7510011-0 1994 Heparin potentiation of the effect of acidic fibroblast growth factor on astrocytes and neurons. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 38-69 7510011-2 1994 The effects of aFGF on both astrocytes and neurons were significantly potentiated by heparin. Heparin 85-92 fibroblast growth factor 1 Rattus norvegicus 15-19 7510011-3 1994 The effect of aFGF (2 ng/ml) with heparin (10 mu g/ml) on the survival of neurons was a hundredfold more potent than that of aFGF (200 ng/ml) without heparin. Heparin 34-41 fibroblast growth factor 1 Rattus norvegicus 14-18 7510011-3 1994 The effect of aFGF (2 ng/ml) with heparin (10 mu g/ml) on the survival of neurons was a hundredfold more potent than that of aFGF (200 ng/ml) without heparin. Heparin 150-157 fibroblast growth factor 1 Rattus norvegicus 14-18 7507688-5 1993 Activity was further defined as fibroblast growth factor (FGF)-like by its strong affinity to heparin, partial neutralisation by antibodies to acidic FGF (aFGF) and partial co-elution with aFGF on salt elution from heparin. Heparin 215-222 fibroblast growth factor 1 Rattus norvegicus 189-193 1351851-8 1992 Heparin, at a concentration that potentiated aFGF-induced neurite outgrowth 100-fold (100 micrograms/ml), did not alter the ability of aFGF to increase S6 phosphorylation or ODC activity. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 45-49 1283855-3 1992 Oral treatment with aFGF, in the presence of heparin, reduced (ED50 value = 30.2 micrograms/kg/day) the acetic-acid-induced gastric ulcer area, when assessed 1 week later. Heparin 45-52 fibroblast growth factor 1 Rattus norvegicus 20-24 1724341-1 1991 We tested acid and basic fibroblast growth factor (aFGF and bFGF), members of the heparin binding FGF family, for their ability to stimulate bone resorption as measured by the release of previously incorporated 45Ca from cultured fetal rat long bones in the presence and absence of heparin. Heparin 82-89 fibroblast growth factor 1 Rattus norvegicus 51-55 1724341-1 1991 We tested acid and basic fibroblast growth factor (aFGF and bFGF), members of the heparin binding FGF family, for their ability to stimulate bone resorption as measured by the release of previously incorporated 45Ca from cultured fetal rat long bones in the presence and absence of heparin. Heparin 282-289 fibroblast growth factor 1 Rattus norvegicus 51-55 1283096-4 1992 A single heparin-Sepharose chromatography of the ultrafiltrates yielded essentially homogenous, biologically active, recombinant rat aFGF or bFGF. Heparin 9-16 fibroblast growth factor 1 Rattus norvegicus 133-137 1724341-6 1991 These results indicate that both aFGF and bFGF can stimulate bone resorption by a prostaglandin-independent mechanism, particularly in the presence of heparin. Heparin 151-158 fibroblast growth factor 1 Rattus norvegicus 33-37 1711938-3 1991 METHODS AND RESULTS: Western-blot analysis of heparin-bound material from neonatal heart extracts identified a single band with a molecular weight of approximately 18 kD for both bFGF and aFGF. Heparin 46-53 fibroblast growth factor 1 Rattus norvegicus 188-192 2331791-8 1990 Heparin-affinity high performance liquid chromatography (HPLC) showed a proportionately greater increase in levels of aFGF than bFGF between the tenth and fortieth postnatal days. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 118-122 1992009-4 1991 Acidic FGF and bFGF from extracts of nervous tissue were found to differ considerably in their relative dependencies upon heparin to potentiate their mitogenic activities: the effect of aFGF was strongly dependent upon heparin, whereas the effect of bFGF was only slightly potentiated by heparin. Heparin 122-129 fibroblast growth factor 1 Rattus norvegicus 186-190 1992009-5 1991 Heparin was also found to stimulate differentially the mitogenic activity of extracts prepared from different areas of the nervous system, indicating that spinal cord, cortex, pituitary, and optic nerve contained different ratios of aFGF to bFGF, whereas sciatic nerve contained extremely high levels of only aFGF. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 233-237 1992009-5 1991 Heparin was also found to stimulate differentially the mitogenic activity of extracts prepared from different areas of the nervous system, indicating that spinal cord, cortex, pituitary, and optic nerve contained different ratios of aFGF to bFGF, whereas sciatic nerve contained extremely high levels of only aFGF. Heparin 0-7 fibroblast growth factor 1 Rattus norvegicus 309-313