PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18247328-9 2008 Moreover, caffeine, a known ATM/ATR kinase inhibitor, relieved the phosphorylation of cdc2 (Tyr-15) by hydroxyurea, but not that by lithium. Caffeine 10-18 cyclin dependent kinase 1 Homo sapiens 86-90 20413215-9 2010 Furthermore, we found the dephosphorylation of Cdk1 at Thr161 induced by GA was abrogated, followed by a remarkable disruption of G2/M arrest when the cells were pre-incubated with caffeine. Caffeine 181-189 cyclin dependent kinase 1 Homo sapiens 47-51 25872745-6 2015 Accordingly, inhibition of ataxia telangiectasia mutated (ATM) by caffeine or siRNA abolished the increase in the p-Chk2 level and restored the delayed CDK1 kinase activity in ChangX cells. Caffeine 66-74 cyclin dependent kinase 1 Homo sapiens 152-156 17169946-7 2006 Recent work in cancer cytogenetics and on the modulation of radiation effects at the chromosome level using changes in gene expression associated with proteins or factors such as caffeine or amifostine treatment during G(2) to M-phase transition, reconfirmed the importance of G(2) chekpoint in determining radiosensitivity and of the cdk1/cyclin-B activity in the conversion of DNA damage into chromatid breaks. Caffeine 179-187 cyclin dependent kinase 1 Homo sapiens 335-339 17182611-4 2007 Addition of caffeine to spindle checkpoint-arrested cells induced >40% apoptosis within 5 h. It also caused proteasome-mediated destruction of cyclin B1, a corresponding reduction in cyclin B1/cdk1 activity, and reduction in MPM-2 reactivity. Caffeine 12-20 cyclin dependent kinase 1 Homo sapiens 196-200 16690595-10 2006 The activity of cycline B1/Cdc2 kinase increased earlier but decayed rapidly in the presence of caffeine. Caffeine 96-104 cyclin dependent kinase 1 Homo sapiens 27-31 12811820-6 2003 Caffeine induced G(2)/M phase cell cycle arrest in NB4 cells in association with the induction of phosphorylation at the Ser-15 residue of p53 and induction of tyrosine phosphorylation of cdc2. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 188-192 16123242-5 2005 In NT embryos treated with caffeine, the activity of p34(cdc2) kinase was significantly (P < 0.05) higher than in those without caffeine at 3 h post-injection. Caffeine 27-35 cyclin dependent kinase 1 Homo sapiens 57-61 15975956-5 2005 The involvement of these molecules in resveratrol-induced S phase was also supported by the studies showing that addition of ATM/ATR inhibitor caffeine reverses resveratrol-caused activation of ATM/ATR-Chk1/2 as well as phosphorylation of Cdc25C, Cdc2 and H2A.X, and S phase arrest. Caffeine 143-151 cyclin dependent kinase 1 Homo sapiens 239-243 12811820-9 2003 These results suggest that caffeine induces cell cycle arrest and apoptosis in association with activation of p53 by a novel pathway to phosphorylate the Ser-15 residue and induction of phosphorylation of cdc 2 in leukemic cells with normal p53. Caffeine 27-35 cyclin dependent kinase 1 Homo sapiens 205-210 11120603-6 2001 In both cell lines, caffeine-induced activation of CDC2 kinase was detected only in cells arrested at G2 and CDC2 kinase-activated cells died exhibiting features of apoptosis. Caffeine 20-28 cyclin dependent kinase 1 Homo sapiens 51-55 12835721-6 2003 (3) 1.5 mM caffeine that relieves cells from G(2)/M arrest also inhibits As(2)O(3)-induced Deltavarphi(m) collapse and apoptosis, (4) 1.0 micro M As(2)O(3) increases the expression of both cyclin B(1) and hCDC20 whereas it inhibits Tyr15 phosphorylation of p34(cdc2). Caffeine 11-19 cyclin dependent kinase 1 Homo sapiens 261-265 12894503-3 2003 Caffeine, a nonspecific inhibitor of ATR, enhanced the cytotoxic effect of cisplatin, modestly decreased the p53 and p21WAF-1 response to cisplatin, and affected the cdc2-p34/cyclin B1 complex by decreasing both cyclin B1 protein accumulation and cdc2-p34 tyrosine 15 phosphorylation. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 166-170 12894503-3 2003 Caffeine, a nonspecific inhibitor of ATR, enhanced the cytotoxic effect of cisplatin, modestly decreased the p53 and p21WAF-1 response to cisplatin, and affected the cdc2-p34/cyclin B1 complex by decreasing both cyclin B1 protein accumulation and cdc2-p34 tyrosine 15 phosphorylation. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 247-251 11278490-8 2001 Caffeine or UCN-01 abolishes the extreme radioresistance with the strong G(2) arrest and at the same time reduces the phosphorylation of Cdc2 in A1-5 cells. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 137-141 11120603-6 2001 In both cell lines, caffeine-induced activation of CDC2 kinase was detected only in cells arrested at G2 and CDC2 kinase-activated cells died exhibiting features of apoptosis. Caffeine 20-28 cyclin dependent kinase 1 Homo sapiens 109-113 11120603-8 2001 Furthermore, cycloheximide inhibited activation of CDK2:cyclin A, which normally precedes CDC2 kinase activation in caffeine-treated cells. Caffeine 116-124 cyclin dependent kinase 1 Homo sapiens 90-94 10683156-3 2000 Arrested cells accumulate cyclin B, the regulatory partner of the mitotic p34(cdc2) kinase, which is normally not abundant until late G(2) phase; treatment of arrested cells with caffeine produces rapid S-phase condensation. Caffeine 179-187 cyclin dependent kinase 1 Homo sapiens 78-82 10770932-6 2000 Interestingly, both DNA damage- and bFGF-induced effects on cdc2 phosphorylation are reverted by caffeine. Caffeine 97-105 cyclin dependent kinase 1 Homo sapiens 60-64 10049063-5 1999 The cell cycle modifications in these cell lines correlated with the increase in radiation-induced p34Cdc2 kinase activity by caffeine. Caffeine 126-134 cyclin dependent kinase 1 Homo sapiens 99-106 10597228-4 1999 In both cases, CDK1 from arrested cells could be reactivated both in vitro by dephosphorylation by recombinant Cdc25B phosphatase and in vivo by caffeine. Caffeine 145-153 cyclin dependent kinase 1 Homo sapiens 15-19 10534572-9 1999 Furthermore, down-regulation of cyclin B and cdk1 was observed in Taxol and Caffeine treated HPAC cells. Caffeine 76-84 cyclin dependent kinase 1 Homo sapiens 45-49 10534572-13 1999 The up-regulation of p21WAF1 and down-regulation of cyclin B and cdk1 suggest their possible roles in G2/M cell cycle arrest caused by both Taxol and Caffeine as reported earlier. Caffeine 150-158 cyclin dependent kinase 1 Homo sapiens 65-69 10428760-11 1999 Caffeine, which abrogates the G2 checkpoint by preventing p34(cdc2) phosphorylation, reduced the accumulation in G2 when added to cultures containing cells on transit to G2, but was ineffective in cells arrested at G2 by sustained cholesterol starvation. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 62-66 10360785-4 1999 The results from these experiments demonstrate that concomitant exposure of HL60 cells to caffeine (2 mM) during irradiation inhibited radiation-induced tyrosine 15 phosphorylation of the G2/M-phase transition checkpoint protein CDC2/p34 kinase and reduced G2/M-phase arrest by 40-46% compared to cells irradiated without caffeine. Caffeine 90-98 cyclin dependent kinase 1 Homo sapiens 229-233 10360785-4 1999 The results from these experiments demonstrate that concomitant exposure of HL60 cells to caffeine (2 mM) during irradiation inhibited radiation-induced tyrosine 15 phosphorylation of the G2/M-phase transition checkpoint protein CDC2/p34 kinase and reduced G2/M-phase arrest by 40-46% compared to cells irradiated without caffeine. Caffeine 322-330 cyclin dependent kinase 1 Homo sapiens 229-233 8837615-0 1996 Selective radiosensitization of p53-deficient cells by caffeine-mediated activation of p34cdc2 kinase. Caffeine 55-63 cyclin dependent kinase 1 Homo sapiens 87-94 9187128-7 1997 In low-dose treated G2-M-arrested FA-C cells, caffeine-dependent activation of cdc2 released the G2-M block but failed to protect against apoptosis, suggesting that apoptosis was not a direct consequence of persistent cdc2 kinase inactivation. Caffeine 46-54 cyclin dependent kinase 1 Homo sapiens 79-83 9371520-8 1997 Caffeine reversed the DNA damage-induced inhibition of Cdc2 by causing dephosphorylation of Cdc2, and this dephosphorylation still occurred even when the Cdc2 feedback loops were blocked with butyrolactone-I. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 55-59 9371520-8 1997 Caffeine reversed the DNA damage-induced inhibition of Cdc2 by causing dephosphorylation of Cdc2, and this dephosphorylation still occurred even when the Cdc2 feedback loops were blocked with butyrolactone-I. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 92-96 9371520-8 1997 Caffeine reversed the DNA damage-induced inhibition of Cdc2 by causing dephosphorylation of Cdc2, and this dephosphorylation still occurred even when the Cdc2 feedback loops were blocked with butyrolactone-I. Caffeine 0-8 cyclin dependent kinase 1 Homo sapiens 92-96 8837615-6 1996 We demonstrate that abrogation of G2 arrest by caffeine-mediated activation of p34cdc2 kinase results in the selective sensitization of p53-deficient primary and tumor cells to irradiation-induced apoptosis. Caffeine 47-55 cyclin dependent kinase 1 Homo sapiens 79-86 8069859-4 1994 Brief caffeine exposures (5 or 10 mM for 1-2 h) caused specific tyrosine dephosphorylation and activation of p34cdc2 kinase, and mitotic progression to a limited extent, in cells which were arrested in G2 following etoposide treatment. Caffeine 6-14 cyclin dependent kinase 1 Homo sapiens 109-116 8800197-8 1996 After UV B-irradiation the inactivation of Cdk1 was less pronounced and only partially diminished in the presence of caffeine. Caffeine 117-125 cyclin dependent kinase 1 Homo sapiens 43-47 1830667-8 1991 In hamster cells, the hyperphosphorylated form of p34cdc2 was complexed with cyclin B and underwent tyrosine dephosphorylation during caffeine-induced premature mitosis. Caffeine 134-142 cyclin dependent kinase 1 Homo sapiens 50-57