PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33020180-2 2020 The essential splicing factor U2AF2 recognizes a polypyrimidine (Py) tract splice site signal and initiates spliceosome assembly. polypyrimidine 49-63 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 30-35 33317029-7 2020 In addition, mutations to lower the score of the polypyrimidine tract (PPT) of exon 7, resulting in lower affinity for U2AF65, were not able to support SF3B1 function, suggesting the importance of U2AF65 in SF3B1 function. polypyrimidine 49-63 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 119-125 33020180-4 2020 We determined crystal structures of the wild-type compared with N196K- or G301D-substituted U2AF2 proteins, each bound to a prototypical AdML polypyrimidine tract, at 1.5, 1.4, or 1.7 A resolutions. polypyrimidine 142-156 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 92-97 28850223-3 2017 The U2AF2 splicing factor recognizes polypyrimidine signals that precede most 3" splice sites as a heterodimer with U2AF1. polypyrimidine 37-51 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 4-9 32343311-1 2020 An essential heterodimer of the U2AF1 and U2AF2 pre-mRNA splicing factors nucleates spliceosome assembly at polypyrimidine (Py) signals preceding the major class of 3" splice sites. polypyrimidine 108-122 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 42-47 27799531-2 2016 The large (U2AF65) and small (U2AF35) subunits of the U2AF heterodimer contact the polypyrimidine tract (Py-tract) and the AG-dinucleotide, respectively. polypyrimidine 83-97 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 11-17 26216990-1 2015 U2 snRNP auxiliary factor 65 kDa (U2AF(65)) is a general splicing factor that contacts polypyrimidine (Py) tract and promotes prespliceosome assembly. polypyrimidine 87-101 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-32 26896798-6 2016 This enhancement of splice acceptor activity is linked to interactions of alphaCPs with the U2 snRNP complex and may be mediated by cooperative interactions with the canonical polypyrimidine tract binding protein, U2AF65. polypyrimidine 176-190 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 214-220 26952537-1 2016 How the essential pre-mRNA splicing factor U2AF(65) recognizes the polypyrimidine (Py) signals of the major class of 3" splice sites in human gene transcripts remains incompletely understood. polypyrimidine 67-81 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 43-50 26216990-1 2015 U2 snRNP auxiliary factor 65 kDa (U2AF(65)) is a general splicing factor that contacts polypyrimidine (Py) tract and promotes prespliceosome assembly. polypyrimidine 87-101 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 34-42 24150945-4 2014 In this study, we demonstrate that the c.-32-13T>G mutation abrogates the binding of the splicing factor U2AF65 to the polypyrimidine tract of exon 2 and that several splicing factors affect exon 2 inclusion, although the only factor capable of acting in the c.-32-13 T>G context is the SR protein family member, SRSF4 (SRp75). polypyrimidine 122-136 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 108-114 24793692-3 2014 Using CFTR alternative exon 9 (E9) as a model, we show here that slowing down elongation can also cause exon skipping by promoting the recruitment of the negative factor ETR-3 onto the UG-repeat at E9 3" splice site, which displaces the constitutive splicing factor U2AF65 from the overlapping polypyrimidine tract. polypyrimidine 294-308 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 266-272 24734879-6 2014 The described spatial distribution reveals the complete spectrum of the unbound forms of U2AF65 that coexist with the small percentage of a preformed RNA-bound domain arrangement required for polypyrimidine-tract recognition by conformational selection. polypyrimidine 192-206 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 89-95 22144099-4 2012 The conformational diversity observed in U2AF65 might also facilitate binding to diverse RNA sequences as found in the polypyrimidine tracts that help define 3" splice sites. polypyrimidine 119-133 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 41-47 23283975-9 2013 To examine the role of splicing factors in mRNA stability, mutations were introduced into the polypyrimidine tract located in the 3" UTR of a mini-gene, and the results demonstrate that U2AF65 binds to such a site and controls the mRNA stability. polypyrimidine 94-108 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 186-192 22730292-5 2012 We provide evidence that YB-1 activates splicing by facilitating the recruitment of U2AF65 to weak polypyrimidine tracts through direct protein-protein interactions. polypyrimidine 99-113 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 84-90 22702716-2 2012 Two consecutive RNA recognition motifs (RRM) of U2AF(65) recognize a polypyrimidine tract at the 3" splice site. polypyrimidine 69-83 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 48-55 23376934-2 2013 The essential pre-mRNA splicing factor U2AF(65) is faced with the paradoxical tasks of accurately targeting polypyrimidine (Py) tracts preceding 3" splice sites while adapting to both cytidine and uridine nucleotides with nearly equivalent frequencies. polypyrimidine 108-122 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 39-46 23017209-0 2012 RBM5 promotes exon 4 skipping of AID pre-mRNA by competing with the binding of U2AF65 to the polypyrimidine tract. polypyrimidine 93-107 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 79-85 23017209-5 2012 Indicative of the underlying mechanism, RBM5 interfered with the binding of U2AF65 to the polypyrimidine tract at the 3"-ss in vitro. polypyrimidine 90-104 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 76-82 21071404-5 2011 Since U2AF65 recognizes the polypyrimidine tract (PPT) and forms a cooperative RNA complex with SF1, we combined the SF1 model with a PPT model computed from high affinity binding sequences for U2AF65. polypyrimidine 28-42 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 6-12 21753750-3 2011 Here we have studied the molecular mechanisms of the recognition of the 3"-splice-site-associated polypyrimidine tract RNA by the large subunit of the human U2 snRNP auxiliary factor (U2AF65) as a key early step in pre-mRNA splicing. polypyrimidine 98-112 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 184-190 21753750-4 2011 We show that the tandem RNA recognition motif domains of U2AF65 adopt two remarkably distinct domain arrangements in the absence or presence of a strong (that is, high affinity) polypyrimidine tract. polypyrimidine 178-192 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 57-63 19144709-6 2009 Taken together, the results suggest that PTBs inhibit MIE gene splicing by competing with U2AF65 for binding to the polypyrimidine tract in pre-mRNA. polypyrimidine 116-130 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 90-96 16982681-7 2006 In addition, competition experiments showed that PTB is able to displace the binding of U2AF65 on the polypyrimidine tract. polypyrimidine 102-116 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 88-94 18067274-0 2008 Alternative modes of binding by U2AF65 at the polypyrimidine tract. polypyrimidine 46-60 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 32-38 18067274-7 2008 The large subunit of the U2 snRNP associated factor (U2AF65), which is essential for splicing of human introns and specifically recognizes the polypyrimidine tract, is also present in Neurospora. polypyrimidine 143-157 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 53-59 18842594-2 2008 Tandem RNA recognition motifs (RRM)s of U2AF65 recognize polypyrimidine tract signals adjacent to 3" splice sites. polypyrimidine 57-71 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 40-46 17188295-1 2007 The essential pre-mRNA splicing factor, U2 auxiliary factor 65KD (U2AF(65)) recognizes the polypyrimidine tract (Py-tract) consensus sequence of the pre-mRNA using two RNA recognition motifs (RRMs), the most prevalent class of eukaryotic RNA-binding domain. polypyrimidine 91-105 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 40-64 17188295-1 2007 The essential pre-mRNA splicing factor, U2 auxiliary factor 65KD (U2AF(65)) recognizes the polypyrimidine tract (Py-tract) consensus sequence of the pre-mRNA using two RNA recognition motifs (RRMs), the most prevalent class of eukaryotic RNA-binding domain. polypyrimidine 91-105 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 66-74 16982681-8 2006 Our results strongly support a model whereby PTB competes with U2AF65 for binding to the polypyrimidine tract. polypyrimidine 89-103 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 63-69 16906767-2 2006 In the current model, U2AF65, bound at the polypyrimidine tract of the intron, recruits U2 snRNP to the branch point sequence by interacting with the U2 snRNP protein SF3b155. polypyrimidine 43-57 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 22-28 16940179-2 2006 U2AF(65) binds to the polypyrimidine tract upstream from the 3" splice site and promotes U2 snRNP binding to the pre-mRNA. polypyrimidine 22-36 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-8 16940179-3 2006 Based on in vitro studies, it has been proposed that U2AF(35) plays a role in assisting U2AF(65) recruitment to nonconsensus polypyrimidine tracts. polypyrimidine 125-139 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 88-95 16818232-0 2006 Structural basis for polypyrimidine tract recognition by the essential pre-mRNA splicing factor U2AF65. polypyrimidine 21-35 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 96-102 16818232-1 2006 The essential pre-mRNA splicing factor, U2AF(65), guides the early stages of splice site choice by recognizing a polypyrimidine (Py) tract consensus sequence near the 3" splice site. polypyrimidine 113-127 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 40-47 11575921-4 2001 SF2/ASF stimulates U2AF65 binding to the suboptimal tat polypyrimidine tract in an ESE3-dependent manner, whereas the exon splicing silencer (ESS3) that is located downstream of the ESE3 inhibits this step. polypyrimidine 56-70 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 19-25 16043505-3 2005 U2AF65 interacts directly with the polypyrimidine tract and promotes binding of U2 snRNP to the pre-mRNA branchpoint, while U2AF35 associates with the conserved AG dinucleotide at the 3" end of the intron and has multiple functions in the splicing process. polypyrimidine 35-49 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-6 16403634-2 2006 Models for PTB activity have ranged from simple binding competition with splicing factor U2AF(65) at regulated polypyrimidine tracts to looping out of repressed exons by binding of PTB to flanking sites. polypyrimidine 111-125 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 89-96 14730023-1 2004 The general splicing factor U2AF(65) recognizes the polypyrimidine tract (Py tract) that precedes 3" splice sites and has three RNA recognition motifs (RRMs). polypyrimidine 52-66 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 28-36 14612416-0 2003 Human immunodeficiency virus type 1 hnRNP A/B-dependent exonic splicing silencer ESSV antagonizes binding of U2AF65 to viral polypyrimidine tracts. polypyrimidine 125-139 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 109-115 14612416-4 2003 We show that hnRNP A/B proteins bound to ESSV are necessary to inhibit E complex assembly by competing with the binding of U2AF65 to the polypyrimidine tracts of repressed 3" splice sites. polypyrimidine 137-151 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 123-129 11438677-2 2001 SF1/mBBP utilizes a "maxi-K homology" (maxi-KH) domain for recognition of the single-stranded BPS and requires a cooperative interaction with splicing factor U2AF65 bound to an adjacent polypyrimidine tract (PPT) for high-affinity binding. polypyrimidine 186-200 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 158-164 10864047-0 2000 Differential recognition of the polypyrimidine-tract by the general splicing factor U2AF65 and the splicing repressor sex-lethal. polypyrimidine 32-46 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 84-90 10567551-0 1999 Evidence for substrate-specific requirement of the splicing factor U2AF(35) and for its function after polypyrimidine tract recognition by U2AF(65). polypyrimidine 103-117 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 139-147 10617206-3 1999 U2AF65 directly contacts the polypyrimidine tract and is required for splicing in vitro. polypyrimidine 29-43 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-6 10567551-2 1999 U2AF(65) binds to the polypyrimidine (Py) tract upstream from the 3" splice site and plays a key role in assisting U2 snRNP recruitment. polypyrimidine 22-36 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-8 9242493-3 1997 Entry of U2 snRNP into the spliceosome is initiated by interaction of the essential splicing factor U2AF65 with the pre-mRNA polypyrimidine tract. polypyrimidine 125-139 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 100-106 10049361-2 1999 It is generally thought that enhancers increase the use of suboptimal splicing signals, and one specific proposal is that enhancers stabilize binding of U2AF65 to weak polypyrimidine (Py) tracts. polypyrimidine 168-182 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 153-159 10449418-1 1999 The large subunit of the human U2 small nuclear ribonucleoprotein particle auxiliary factor (hU2AF(65)) is an essential RNA-splicing factor required for the recognition of the polypyrimidine tract immediately upstream of the 3" splice site. polypyrimidine 176-190 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 93-102 9512519-3 1998 The mammalian ortholog (mBBP/SF1) also binds specifically to branchpoint sequences and interacts with the well studied mammalian splicing factor U2AF65, which binds to the adjacent polypyrimidine (PY) tract. polypyrimidine 181-195 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 145-151 9512519-4 1998 In this paper we demonstrate that the mBBP/SF1-U2AF65 interaction promotes cooperative binding to a branchpoint sequence-polypyrimidine tract-containing RNA, and we suggest that this cooperative RNA binding contributes to initial recognition of the branchpoint sequence (BPS) during pre-mRNA splicing. polypyrimidine 121-135 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 47-53 1388271-1 1992 U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). polypyrimidine 181-195 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 147-153 7493318-4 1995 U2AF65, an essential splicing factor that binds to the adjacent polypyrimidine tract, crosslinks to the branch point at the earliest stage of spliceosome formation in an ATP-independent manner. polypyrimidine 64-78 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-6 8781232-1 1996 The mammalian splicing factor U2AF65 binds to the polypyrimidine tract adjacent to the 3" splice site and promotes assembly of U2 small nuclear ribonucleoprotein on the upstream branch point, an interaction that involves base pairing with U2 small nuclear RNA (snRNA). polypyrimidine 50-64 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 30-36 8781232-2 1996 U2AF65 contains an RNA binding domain, required for interaction with the polypyrimidine tract, and an arginine-serine-rich (RS) region, required for U2 snRNP recruitment and splicing. polypyrimidine 73-87 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-6 8781232-3 1996 Here it is reported that binding of U2AF65 to the polypyrimidine tract directed the RS domain to contact the branch point and promoted U2 snRNA-branch point base pairing even in the absence of other splicing factors. polypyrimidine 50-64 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 36-42 8713106-2 1996 One such factor, the U2 snRNP auxiliary factor large subunit (U2AF65), contains three RNP-CS motifs each of which is required for high affinity binding to polypyrimidine tracts. polypyrimidine 155-169 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 21-60 8713106-2 1996 One such factor, the U2 snRNP auxiliary factor large subunit (U2AF65), contains three RNP-CS motifs each of which is required for high affinity binding to polypyrimidine tracts. polypyrimidine 155-169 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 62-68 7761834-1 1995 In higher eukaryotes, the polypyrimidine-tract (Py-tract) adjacent to the 3" splice site is recognized by several proteins, including the essential splicing factor U2AF65, the splicing regulator Sex-lethal (Sxl), and polypyrimidine tract-binding protein (PTB), whose function is unknown. polypyrimidine 26-40 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 164-170 8643376-6 1995 In fact, our data show a reciprocal correlation between the strength of a polypyrimidine tract, defined by its affinity for U2AF65K in vitro, and the efficiency of splicing in Ad NE. polypyrimidine 74-88 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 124-130 8206372-5 1994 The A-allele-specific mutation at position +6 is able to interrupt the perfect complementarity of the intron-4 splice donor signal (positions one to eight) with U1-snRNA, which may then no longer be able to compensate for a rather weak exon-4 upstream splice acceptor sequence in facilitating the initial binding of U2 auxiliary factor/65-kDa (U2AF65) to that polypyrimidine tract. polypyrimidine 360-374 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 316-342 8206372-5 1994 The A-allele-specific mutation at position +6 is able to interrupt the perfect complementarity of the intron-4 splice donor signal (positions one to eight) with U1-snRNA, which may then no longer be able to compensate for a rather weak exon-4 upstream splice acceptor sequence in facilitating the initial binding of U2 auxiliary factor/65-kDa (U2AF65) to that polypyrimidine tract. polypyrimidine 360-374 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 344-350 35524551-1 2022 The essential pre-mRNA splicing factor U2AF2 (also called U2AF65) identifies polypyrimidine (Py) tract signals of nascent transcripts, despite length and sequence variations. polypyrimidine 77-91 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 39-44 34868124-2 2021 U2AF65 is a crucial splicing cofactor, and the two typical RNA-recognition motifs at its center recognize and bind the polypyrimidine sequence located between the intron branch site and the 3"-splice site. polypyrimidine 119-133 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 0-6 35524551-1 2022 The essential pre-mRNA splicing factor U2AF2 (also called U2AF65) identifies polypyrimidine (Py) tract signals of nascent transcripts, despite length and sequence variations. polypyrimidine 77-91 U2 small nuclear RNA auxiliary factor 2 Homo sapiens 58-64