PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24720124-4 2013 In the present study we used IgE sera, obtained from patients manifesting different symptoms of wheat allergies, for immunobloting analysis, to prove the specificity of immunological reaction between IgE antibodies and individual gliadins and glutenins, separated by SDS-PAGE. Sodium Dodecyl Sulfate 267-270 immunoglobulin heavy constant epsilon Homo sapiens 200-203 16817976-7 2006 IgE-binding components in extracts from the two types of potato were identified by SDS-PAGE and IgE-immunoblotting. Sodium Dodecyl Sulfate 83-86 immunoglobulin heavy constant epsilon Homo sapiens 0-3 18061650-11 2008 SDS-PAGE immunoblotting-inhibition showed a complete IgE-binding inhibition when sera from these patients were preincubated with dog dander extract. Sodium Dodecyl Sulfate 0-3 immunoglobulin heavy constant epsilon Homo sapiens 53-56 15716601-8 2005 Six IgE binding components to the extract (10, 25, 28, 36, 50, and 90 kDa) were detected using SDS-PAGE and immunoblot analysis. Sodium Dodecyl Sulfate 95-98 immunoglobulin heavy constant epsilon Homo sapiens 4-7 15932383-7 2005 The subfraction with optimum IgE reactivity was studied by activity gel, native and nonreducing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Sodium Dodecyl Sulfate 96-119 immunoglobulin heavy constant epsilon Homo sapiens 29-32 15932383-7 2005 The subfraction with optimum IgE reactivity was studied by activity gel, native and nonreducing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Sodium Dodecyl Sulfate 156-159 immunoglobulin heavy constant epsilon Homo sapiens 29-32 15301307-4 2004 Eosinophilic gastroenteritis was due to bovine serum albumin (BSA) hypersensitivity, confirmed with skin tests, serum levels to specific IgE and a SDS-PAGE IgE-immunoblotting. Sodium Dodecyl Sulfate 147-150 immunoglobulin heavy constant epsilon Homo sapiens 156-159 16433207-6 2005 ImmunoCAP-inhibition and SDS-PAGE-IgE-Immunoblotting-inhibition probed with human sera, showed a total inhibition of specific IgE reactions by the heterologous antigens. Sodium Dodecyl Sulfate 25-28 immunoglobulin heavy constant epsilon Homo sapiens 34-37 16433207-6 2005 ImmunoCAP-inhibition and SDS-PAGE-IgE-Immunoblotting-inhibition probed with human sera, showed a total inhibition of specific IgE reactions by the heterologous antigens. Sodium Dodecyl Sulfate 25-28 immunoglobulin heavy constant epsilon Homo sapiens 126-129 15608387-5 2004 IgE-binding components were identified by means of SDS-PAGE and IgE-immunoblotting. Sodium Dodecyl Sulfate 51-54 immunoglobulin heavy constant epsilon Homo sapiens 0-3 10400861-9 1999 IgE was bound most intensively to a protein with an apparent molecular weight of 25 kd in SDS-PAGE and with a determined molecular weight of 18.7 kd. Sodium Dodecyl Sulfate 90-93 immunoglobulin heavy constant epsilon Homo sapiens 0-3 11398091-8 2001 To identify IgE-binding components within P chinensis extracts, 12% SDS-PAGE with immunoblot analysis was applied. Sodium Dodecyl Sulfate 68-71 immunoglobulin heavy constant epsilon Homo sapiens 12-15 11737676-3 2001 Sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting revealed specific immunoglobulin E (IgE) antibodies in the patient"s serum against a 94,000 molecular-weight antigenic band. Sodium Dodecyl Sulfate 0-23 immunoglobulin heavy constant epsilon Homo sapiens 104-126 10435472-4 1999 METHODS: Identification of IgE-binding components from O. europaea pollen extracts was elucidated by inhibition of SDS-PAGE immunoblotting using recombinant profilin (Ole e 2) and Ole e 1 molecules. Sodium Dodecyl Sulfate 115-118 immunoglobulin heavy constant epsilon Homo sapiens 27-30 10496150-4 1998 Characterization of allergens by SDS-PAGE-immunoblotting using sera from asthmatic patients showed that there was an IgE cross-reactivity among the 33 KDa group major allergens of P. citrinum, P. notatum and P. brevicompactum. Sodium Dodecyl Sulfate 33-36 immunoglobulin heavy constant epsilon Homo sapiens 117-120 9756208-8 1998 Immunoblotting after SDS-PAGE disclosed the presence of four prominent IgE-binding polypeptides in pork kidney (200, 90, 57, and 47 kDa), two in gut (57 and 27 kDa), and three in meat (51, 40, and 28-30 kDa), apart from other weaker radiostained bands in each extract. Sodium Dodecyl Sulfate 21-24 immunoglobulin heavy constant epsilon Homo sapiens 71-74 9389303-7 1997 RESULTS: Serum IgE values were elevated more than 2 SDs above control age-matched mean values in 17 of 43 patients (40%). Sodium Dodecyl Sulfate 52-55 immunoglobulin heavy constant epsilon Homo sapiens 15-18 8926066-2 1996 One of the targets of the IgE response is an adult-worm sodium dodecyl sulfate-polyacrylamide gel electrophoresis band of 22 kDa (Sm22), which contains an antigen(s) located within the tegument and gut lining of adult worms and relatively late schistosomula life cycle stages only. Sodium Dodecyl Sulfate 56-78 immunoglobulin heavy constant epsilon Homo sapiens 26-29 9048527-4 1997 METHODS: IgE reactivity was measured in eight clinically codfish-allergic adult patients, confirmed by double-blind, placebo-controlled challenges with fresh raw codfish, and in 30 codfish-tolerant control subjects, by means of skin prick test, histamine release test, specific IgE tests, SDS-PAGE, and immunoblotting. Sodium Dodecyl Sulfate 289-292 immunoglobulin heavy constant epsilon Homo sapiens 9-12 9076810-4 1997 Here we report the isolation of a 22 kDa SDS-PAGE band, its incorporation into ELISA and the demonstration that levels of human anti-22 kDa IgE had a significant negative correlation with intensities of subsequent reinfection. Sodium Dodecyl Sulfate 41-44 immunoglobulin heavy constant epsilon Homo sapiens 140-143 8012858-2 1994 The SDS-PAGE under reduced conditions followed by western blotting analysis revealed 10 human IgE-binding components with molecular masses ranging from 15 to 143 kDa. Sodium Dodecyl Sulfate 4-7 immunoglobulin heavy constant epsilon Homo sapiens 94-97 8612113-7 1996 IgE responses from 19 patients were investigated by means of CAP RAST assay and SDS PAGE immunoblotting. Sodium Dodecyl Sulfate 80-83 immunoglobulin heavy constant epsilon Homo sapiens 0-3 7573842-3 1995 CRIE detected from one to four important allergens, while SDS showed up to four bands that bound IgE from a number of patient sera. Sodium Dodecyl Sulfate 58-61 immunoglobulin heavy constant epsilon Homo sapiens 97-100 7573842-4 1995 CRIE and SDS-PAGE immunoblotting did not recognize the same number of important allergens in the individual weeds, and the number of allergens detected by the two methods differed considerably, suggesting that IgE-binding epitopes may be denatured during SDS-PAGE. Sodium Dodecyl Sulfate 9-12 immunoglobulin heavy constant epsilon Homo sapiens 210-213 7573842-4 1995 CRIE and SDS-PAGE immunoblotting did not recognize the same number of important allergens in the individual weeds, and the number of allergens detected by the two methods differed considerably, suggesting that IgE-binding epitopes may be denatured during SDS-PAGE. Sodium Dodecyl Sulfate 255-258 immunoglobulin heavy constant epsilon Homo sapiens 210-213 7515604-2 1994 SDS-PAGE analysis of two batches of Kentucky bluegrass pollen extracts demonstrated that there was considerable variability in allergen content of extracts, which in turn affected quantitation of specific IgE antibodies by different immunoassay procedures. Sodium Dodecyl Sulfate 0-3 immunoglobulin heavy constant epsilon Homo sapiens 205-208 8404825-7 1993 SDS-PAGE revealed that the protein which bound the highest percentage of IgE from the sera of the allergic bakers is a 27 kDa albumin. Sodium Dodecyl Sulfate 0-3 immunoglobulin heavy constant epsilon Homo sapiens 73-76 8238811-4 1993 SDS-PAGE/immunoblotting showed the presence of mainly the same IgE-binding bands in the two extracts. Sodium Dodecyl Sulfate 0-3 immunoglobulin heavy constant epsilon Homo sapiens 63-66 7694449-6 1993 An SDS-PAGE of the extract revealed a major protein band with an estimated mol wt of 52,000 d. This band had the highest IgE-binding capacity as shown by immunoblotting. Sodium Dodecyl Sulfate 3-6 immunoglobulin heavy constant epsilon Homo sapiens 121-124 3679560-6 1987 Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting of CDE proved the presence of, at least, 9 polypeptides with affinity to IgE, those with molecular weights of 18,000 and 22,000 daltons being the most active ones. Sodium Dodecyl Sulfate 12-34 immunoglobulin heavy constant epsilon Homo sapiens 162-165 1819644-1 1991 The IgE-binding proteins in soybeans were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the fractionated soybean proteins probed with the sera of the patients with atopic dermatitis. Sodium Dodecyl Sulfate 59-81 immunoglobulin heavy constant epsilon Homo sapiens 4-7 1676206-1 1991 IgE-binding components in crude extracts of Pityrosporum orbiculare were separated by sodium dodecylsulphate polyacrylamide gel electrophoresis and transferred to Immobilon membrane. Sodium Dodecyl Sulfate 86-108 immunoglobulin heavy constant epsilon Homo sapiens 0-3 2324418-7 1990 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blotting revealed a 30 kda of protein that was recognized by the IgE antibodies in the patient"s serum. Sodium Dodecyl Sulfate 0-22 immunoglobulin heavy constant epsilon Homo sapiens 143-146 7680928-5 1993 Immunoblot experiments (n = 22) using SDS-PAGE demonstrated five different locations of IgE binding in 15 sera: 18, 35, 46, 54 and 66 kD. Sodium Dodecyl Sulfate 38-41 immunoglobulin heavy constant epsilon Homo sapiens 88-91 1282843-1 1992 In this paper we show the reactivity of monoclonal antibodies (mAbs) and human IgE with Fel d I from different allergen sources in reduced SDS-PAGE immunoblots. Sodium Dodecyl Sulfate 139-142 immunoglobulin heavy constant epsilon Homo sapiens 79-82 1705952-5 1991 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis resolved protein/peptide bands of both SS and DP also bound serum IgE from dust-mite-allergic patients following immunoblotting. Sodium Dodecyl Sulfate 0-22 immunoglobulin heavy constant epsilon Homo sapiens 124-127