PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 6746643-11 1984 The antibody is directed against a single protein which migrated between GPIIb and GPIIa after sodium dodecyl sulfate gel electrophoresis. Sodium Dodecyl Sulfate 95-117 integrin subunit alpha 2b Homo sapiens 73-78 1702007-4 1990 In contrast, immunoblotting demonstrated that Yam, Lin and Kl bound to SDS-denatured GP IIb, while MO did not. Sodium Dodecyl Sulfate 71-74 integrin subunit alpha 2b Homo sapiens 85-91 2767240-3 1989 Employing electrophoretically (SDS-PAGE) separated normal platelet membrane proteins in an immunoblot assay, serum IgG and IgM antibodies were found to be directed to antigenic determinants situated on glycoprotein GP IIb (140 kDa) in all patients, but also on smaller proteins of molecular weights between 30 and 52 kDa. Sodium Dodecyl Sulfate 31-34 integrin subunit alpha 2b Homo sapiens 215-221 3569161-1 1987 Sedimentation equilibrium and low-angle laser-light scattering were used to determine the molar mass of the glycoprotein moieties in the complexes of sodium dodecyl sulphate with the human platelet membrane glycoproteins IIb (GPIIb), IIIa (GPIIIa), and the alpha (GPIIb alpha) and beta (GPIIb beta) subunits of GPIIb. Sodium Dodecyl Sulfate 150-173 integrin subunit alpha 2b Homo sapiens 264-269 3569161-1 1987 Sedimentation equilibrium and low-angle laser-light scattering were used to determine the molar mass of the glycoprotein moieties in the complexes of sodium dodecyl sulphate with the human platelet membrane glycoproteins IIb (GPIIb), IIIa (GPIIIa), and the alpha (GPIIb alpha) and beta (GPIIb beta) subunits of GPIIb. Sodium Dodecyl Sulfate 150-173 integrin subunit alpha 2b Homo sapiens 264-269 1856198-6 1991 A single radiolabeled component (120 kDa) was identified and shown to be identical with the alpha subunit of GPIIb based on two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blotting with anti-GPIIb monoclonal antibodies, by isoelectric focusing (pI 4.5-5.5), by immunoaffinity adsorption using monoclonal anti-GPIIb/IIIa antibodies coupled to Sepharose, and by crossed immunoelectrophoresis. Sodium Dodecyl Sulfate 140-162 integrin subunit alpha 2b Homo sapiens 109-114 2611137-6 1989 In addition, both CIE in the presence of the EDTA and two-dimensional sodium dodecyl sulphate (SDS) gel electrophoresis showed the presence of two forms of GPIIb. Sodium Dodecyl Sulfate 70-93 integrin subunit alpha 2b Homo sapiens 156-161 2611137-6 1989 In addition, both CIE in the presence of the EDTA and two-dimensional sodium dodecyl sulphate (SDS) gel electrophoresis showed the presence of two forms of GPIIb. Sodium Dodecyl Sulfate 95-98 integrin subunit alpha 2b Homo sapiens 156-161 2465020-5 1989 In addition, immunoblots made after two-dimensional nonreduced-reduced SDS-polyacrylamide gel electrophoresis (SDS-PAGE) directly demonstrated that both 65 kD fragments had a molecular weight of 80 kD under nonreducing conditions; this provides evidence that these fragments were one and the same, and were derived from GP IIb alpha. Sodium Dodecyl Sulfate 111-114 integrin subunit alpha 2b Homo sapiens 320-326 3355897-3 1988 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of her platelets indicates that they contain two types of GPIIb molecules: one with an abnormal mol wt (122 kd, unreduced; 128 kd, reduced) and one with a normal mol wt (128 kd, unreduced; 118 kd, reduced). Sodium Dodecyl Sulfate 0-22 integrin subunit alpha 2b Homo sapiens 116-121 3629560-5 1987 PAS-stained SDS-PAGE of her whole platelets showed markedly decreased GP IIb and IIIa. Sodium Dodecyl Sulfate 12-15 integrin subunit alpha 2b Homo sapiens 70-76 3629560-9 1987 Immunoprecipitation lines of her GP IIb/IIIa complex were excised from unstained CIE using anti-GP IIb/IIIa antibody and subjected to the silver-stained reduced SDS-PAGE, which showed two protein bands with molecular weights of 125KD and 108KD, corresponding to GP IIb alpha and GP IIIa, respectively. Sodium Dodecyl Sulfate 161-164 integrin subunit alpha 2b Homo sapiens 33-39 3801670-2 1987 GP IIb and IIIa were further separated in the presence of sodium dodecyl sulfate (SDS) by gel filtration high-performance liquid chromatography (HPLC). Sodium Dodecyl Sulfate 58-80 integrin subunit alpha 2b Homo sapiens 0-6 3801670-2 1987 GP IIb and IIIa were further separated in the presence of sodium dodecyl sulfate (SDS) by gel filtration high-performance liquid chromatography (HPLC). Sodium Dodecyl Sulfate 82-85 integrin subunit alpha 2b Homo sapiens 0-6 3569161-1 1987 Sedimentation equilibrium and low-angle laser-light scattering were used to determine the molar mass of the glycoprotein moieties in the complexes of sodium dodecyl sulphate with the human platelet membrane glycoproteins IIb (GPIIb), IIIa (GPIIIa), and the alpha (GPIIb alpha) and beta (GPIIb beta) subunits of GPIIb. Sodium Dodecyl Sulfate 150-173 integrin subunit alpha 2b Homo sapiens 226-231 3569161-1 1987 Sedimentation equilibrium and low-angle laser-light scattering were used to determine the molar mass of the glycoprotein moieties in the complexes of sodium dodecyl sulphate with the human platelet membrane glycoproteins IIb (GPIIb), IIIa (GPIIIa), and the alpha (GPIIb alpha) and beta (GPIIb beta) subunits of GPIIb. Sodium Dodecyl Sulfate 150-173 integrin subunit alpha 2b Homo sapiens 264-269 2999274-3 1985 The quantity of iodine 125-labeled glycoprotein IIb (GPIIb) and glycoprotein IIIa (GPIIIa) on the platelets of these patients was considerably less than normal but was detectable by immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and autoradiography. Sodium Dodecyl Sulfate 203-225 integrin subunit alpha 2b Homo sapiens 53-58 7229034-4 1981 Major periodate-Schiff staining bands corresponding to membrane GP IIb, IIIa, and IIIb were clearly detected and their presence was confirmed by two-dimensional SDS-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 161-164 integrin subunit alpha 2b Homo sapiens 64-70 6229297-11 1984 Autoradiographs from SDS-PAGE electrophoresis of the Staph-A-bound proteins shows two radioactive bands consistent in size with GPIIb and GPIIIa. Sodium Dodecyl Sulfate 21-24 integrin subunit alpha 2b Homo sapiens 128-133 7354135-5 1980 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or protein 16 cut out from immunoplates showed two (125)I-labeled glycoprotein bands, which migrate as GP IIb and GP IIIa. Sodium Dodecyl Sulfate 0-22 integrin subunit alpha 2b Homo sapiens 172-178 7354135-5 1980 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or protein 16 cut out from immunoplates showed two (125)I-labeled glycoprotein bands, which migrate as GP IIb and GP IIIa. Sodium Dodecyl Sulfate 59-62 integrin subunit alpha 2b Homo sapiens 172-178 12038797-3 2002 Electrophoretic analyses demonstrated that tirofiban protected GpIIb/IIIa from SDS-induced subunit dissociation. Sodium Dodecyl Sulfate 79-82 integrin subunit alpha 2b Homo sapiens 63-68 7506568-3 1993 This antibody (i) precipitated GPIIb-IIIa from platelet Triton X-100-lysate, (ii) recognized the GPIIIa band in Western blotting of platelet SDS-lysate, and (iii) did not react with platelets from a Glanzmann"s thrombasthenia patient lacking GPIIb-IIIa. Sodium Dodecyl Sulfate 141-144 integrin subunit alpha 2b Homo sapiens 31-36 8765234-0 1996 NOE-derived conformation of GRGDSP cell adhesion recognition site in the presence of SDS micelles and integrin receptor GPIIB/IIIA. Sodium Dodecyl Sulfate 85-88 integrin subunit alpha 2b Homo sapiens 120-125 8549808-0 1996 RGD induces conformational transition in purified platelet integrin GPIIb/IIIa-SDS system yielding multiple binding states for fibrinogen gamma-chain C-terminal peptide. Sodium Dodecyl Sulfate 79-82 integrin subunit alpha 2b Homo sapiens 68-73 8549808-2 1996 NMR studies of gamma 12 in the presence of purified GPIIb/IIIa in SDS/water solution have demonstrated the presence of two gamma 12 binding states, one of which is eliminated by GRGDSP (RGD) up to a RGD: gamma 12 ratio of 2:1. Sodium Dodecyl Sulfate 66-69 integrin subunit alpha 2b Homo sapiens 52-57