PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3402391-2 1988 Metabolic labeling, followed by immunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the rat brain and liver cells synthesized and secreted the following immunoreactive IGF-II peptides: 19, 11, 10, and 8.7 kilodaltons (kd), whereas the rat pituitary secreted the 10 and 8.7 kd peptides. Sodium Dodecyl Sulfate 56-78 insulin-like growth factor 2 Rattus norvegicus 212-218 2995005-9 1985 When [125I]IGF-II was cross-linked to GH3 cells and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two receptors were identified. Sodium Dodecyl Sulfate 64-86 insulin-like growth factor 2 Rattus norvegicus 11-17 6321506-7 1984 The proteins eluted from the region of the gel when cross-linked to 125I-IGF-II with disuccinimidyl suberate and electrophoresed in the presence of sodium dodecyl sulfate have the same molecular weight, Mr, as the previously described IGF-II receptor. Sodium Dodecyl Sulfate 148-170 insulin-like growth factor 2 Rattus norvegicus 73-79 6321506-7 1984 The proteins eluted from the region of the gel when cross-linked to 125I-IGF-II with disuccinimidyl suberate and electrophoresed in the presence of sodium dodecyl sulfate have the same molecular weight, Mr, as the previously described IGF-II receptor. Sodium Dodecyl Sulfate 148-170 insulin-like growth factor 2 Rattus norvegicus 235-241 6321506-10 1984 Thus, quantitative procedures that separate native proteins based on their size and charge have provided information which is in good agreement with the results of studies of the IGF-II-receptor by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and supports the premise that it is a monomeric single chained glycoprotein, constrained by intradisulfide bonds and with a mass of 250 kDa. Sodium Dodecyl Sulfate 198-220 insulin-like growth factor 2 Rattus norvegicus 179-185 6305954-15 1983 This value for the Mr is similar to the mass of 220,000 or 250,000 Dal determined by cross-linking 125I-IGF-II to the membrane- or detergent-solubilized receptors with disuccimidyl suberate and separating the complex by electrophoresis in sodium dodecyl sulfate-containing polyacrylamide gels in the absence or presence of dithiothreitol, respectively. Sodium Dodecyl Sulfate 239-261 insulin-like growth factor 2 Rattus norvegicus 104-110 7530649-7 1995 The acid eluate and the acid-stripped flow-through predominantly formed 50-kDa complexes with [125I]IGF-II when affinity-cross-linked and examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; these complexes were precipitated by antibodies to rat IGFBP-3. Sodium Dodecyl Sulfate 150-172 insulin-like growth factor 2 Rattus norvegicus 100-106