PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
6140073-7	1983	Also, immunochemical detection of glutamine synthetase in brain homogenates fractionated by SDS-polyacrylamide gel electrophoresis and transferred to diazobenzyloxymethyl paper showed that the increase in enzyme activity was due to induction of protein synthesis.	Sodium Dodecyl Sulfate	92-95	glutamate-ammonia ligase	Rattus norvegicus	34-54	
3087572-9	1986	The specificity of monoclonal antibodies against GAD were established from the following criteria: positive on ELISA test using homogeneous GAD as antigen; formation of GAD--anti-GAD IgG complex as indicated from gel filtration chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis; and specific recognition of GAD subunit in a partially purified GAD preparation in Western immunoblotting test.	Sodium Dodecyl Sulfate	247-269	glutamate-ammonia ligase	Rattus norvegicus	49-52	
12590937-1	2003	The post-mortem stability of the GABA synthesizing enzyme glutamate decarboxylase (GAD) was studied by using SDS-PAGE and quantitative immunoblotting to measure the rates of degradation of GAD in the cerebral cortex, hippocampus, and cerebellum of rats and mice as a function of time after death.	Sodium Dodecyl Sulfate	109-112	glutamate-ammonia ligase	Rattus norvegicus	58-81	
10403432-1	1999	The levels of the two isoforms of glutamate decarboxylase (GAD) were measured in 12 regions of adult rat brain and three regions of mouse brain by sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with an antiserum that recognizes the identical C-terminal sequence in both isoforms from both species.	Sodium Dodecyl Sulfate	147-168	glutamate-ammonia ligase	Rattus norvegicus	34-57	
10403432-1	1999	The levels of the two isoforms of glutamate decarboxylase (GAD) were measured in 12 regions of adult rat brain and three regions of mouse brain by sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with an antiserum that recognizes the identical C-terminal sequence in both isoforms from both species.	Sodium Dodecyl Sulfate	147-168	glutamate-ammonia ligase	Rattus norvegicus	59-62	
1973454-6	1990	Analysis of labeled GAD by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed two labeled proteins with apparent molecular masses of 59 and 63 kDa.	Sodium Dodecyl Sulfate	27-49	glutamate-ammonia ligase	Rattus norvegicus	20-23	
1973454-6	1990	Analysis of labeled GAD by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed two labeled proteins with apparent molecular masses of 59 and 63 kDa.	Sodium Dodecyl Sulfate	51-54	glutamate-ammonia ligase	Rattus norvegicus	20-23	
1973454-8	1990	Three peaks of GAD activity (termed peaks I, II, and III) were separated by chromatography on phenyl-Sepharose, labeled with [32P]pyridoxal-P, purified by immunoaffinity chromatography, and analyzed by SDS-polyacrylamide gel electrophoresis.	Sodium Dodecyl Sulfate	202-205	glutamate-ammonia ligase	Rattus norvegicus	15-18