PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10403432-1 1999 The levels of the two isoforms of glutamate decarboxylase (GAD) were measured in 12 regions of adult rat brain and three regions of mouse brain by sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with an antiserum that recognizes the identical C-terminal sequence in both isoforms from both species. Sodium Dodecyl Sulfate 147-168 glutamate-ammonia ligase Rattus norvegicus 34-57 12590937-1 2003 The post-mortem stability of the GABA synthesizing enzyme glutamate decarboxylase (GAD) was studied by using SDS-PAGE and quantitative immunoblotting to measure the rates of degradation of GAD in the cerebral cortex, hippocampus, and cerebellum of rats and mice as a function of time after death. Sodium Dodecyl Sulfate 109-112 glutamate-ammonia ligase Rattus norvegicus 58-81 10403432-1 1999 The levels of the two isoforms of glutamate decarboxylase (GAD) were measured in 12 regions of adult rat brain and three regions of mouse brain by sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with an antiserum that recognizes the identical C-terminal sequence in both isoforms from both species. Sodium Dodecyl Sulfate 147-168 glutamate-ammonia ligase Rattus norvegicus 59-62 1973454-6 1990 Analysis of labeled GAD by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed two labeled proteins with apparent molecular masses of 59 and 63 kDa. Sodium Dodecyl Sulfate 27-49 glutamate-ammonia ligase Rattus norvegicus 20-23 1973454-6 1990 Analysis of labeled GAD by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis showed two labeled proteins with apparent molecular masses of 59 and 63 kDa. Sodium Dodecyl Sulfate 51-54 glutamate-ammonia ligase Rattus norvegicus 20-23 1973454-8 1990 Three peaks of GAD activity (termed peaks I, II, and III) were separated by chromatography on phenyl-Sepharose, labeled with [32P]pyridoxal-P, purified by immunoaffinity chromatography, and analyzed by SDS-polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 202-205 glutamate-ammonia ligase Rattus norvegicus 15-18 6140073-7 1983 Also, immunochemical detection of glutamine synthetase in brain homogenates fractionated by SDS-polyacrylamide gel electrophoresis and transferred to diazobenzyloxymethyl paper showed that the increase in enzyme activity was due to induction of protein synthesis. Sodium Dodecyl Sulfate 92-95 glutamate-ammonia ligase Rattus norvegicus 34-54 3087572-9 1986 The specificity of monoclonal antibodies against GAD were established from the following criteria: positive on ELISA test using homogeneous GAD as antigen; formation of GAD--anti-GAD IgG complex as indicated from gel filtration chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis; and specific recognition of GAD subunit in a partially purified GAD preparation in Western immunoblotting test. Sodium Dodecyl Sulfate 247-269 glutamate-ammonia ligase Rattus norvegicus 49-52