PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
3545083-2	1987	The purity of human CSF-1 was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a diffuse single band of Mr 42,000-50,000 and by N-terminal amino acid analysis of glutamate residue.	Sodium Dodecyl Sulfate	46-68	colony stimulating factor 1	Homo sapiens	20-25	
3496105-2	1987	We have isolated 8 mg of purified CSF-1 which migrated as a single band under non-reducing conditions in SDS-PAGE (staining with Coomassie Blue and the sensitive silver techniques).	Sodium Dodecyl Sulfate	105-108	colony stimulating factor 1	Homo sapiens	34-39	
2834139-6	1988	The isolated CSF-1 which exhibits a one band pattern on SDS-PAGE under non-reducing conditions after Coomassie Blue and silver stainings.	Sodium Dodecyl Sulfate	56-59	colony stimulating factor 1	Homo sapiens	13-18	
3545083-2	1987	The purity of human CSF-1 was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a diffuse single band of Mr 42,000-50,000 and by N-terminal amino acid analysis of glutamate residue.	Sodium Dodecyl Sulfate	105-108	colony stimulating factor 1	Homo sapiens	20-25	
3545083-7	1987	However, CSF-1 activity was inhibited totally by the combination of 10 mM DTT and 1 mM SDS.	Sodium Dodecyl Sulfate	87-90	colony stimulating factor 1	Homo sapiens	9-14	
3545083-8	1987	Under denaturing and reducing conditions, CSF-1 appeared on SDS-PAGE as a single protein band of Mr 21,000-25,000 and concurrently lost its activity, indicating that human CSF-1 possibly consists of two similar subunits and that the intact quaternary structure is essential for the biological activity.	Sodium Dodecyl Sulfate	60-63	colony stimulating factor 1	Homo sapiens	42-47	
8591991-6	1996	After labeling the cellular proteins with [35S] met/cys or [35S] SO4(2-), CSF-1 was immunoprecipitated and analyzed by SDS-PAGE.	Sodium Dodecyl Sulfate	119-122	colony stimulating factor 1	Homo sapiens	74-79	
9973638-5	1998	Gel fractions extracted from SDS-PAGE had macrophage colony-stimulating factor (M-CSF)-specific amino acid sequences.	Sodium Dodecyl Sulfate	29-32	colony stimulating factor 1	Homo sapiens	42-78	
9973638-5	1998	Gel fractions extracted from SDS-PAGE had macrophage colony-stimulating factor (M-CSF)-specific amino acid sequences.	Sodium Dodecyl Sulfate	29-32	colony stimulating factor 1	Homo sapiens	80-85	
6609780-5	1984	The M-CSF had an apparent molecular weight of 77,000 in the absence of 0.1% SDS and 49,000 in its presence.	Sodium Dodecyl Sulfate	76-79	colony stimulating factor 1	Homo sapiens	4-9	
8920951-9	1996	The electrophoretic mobilities of the cleaved dimeric ectodomains of these mutant CSF-1 proteins on SDS-PAGE exhibited distinctly different patterns as compared with the wild type.	Sodium Dodecyl Sulfate	100-103	colony stimulating factor 1	Homo sapiens	82-87	
8591991-12	1996	CSF-1 released with trypsin from the membrane fraction yielded on SDS-PAGE a band with MW of 60 and 30 kd under nonreducing and reducing conditions, respectively.	Sodium Dodecyl Sulfate	66-69	colony stimulating factor 1	Homo sapiens	0-5	
8454579-1	1993	The recombinant human carboxy-terminal-truncated macrophage colony-stimulating factor ([3-153]M-CSF) consists of 302 amino acid residues and has a molecular mass of about 32 kDa, as estimated by SDS-PAGE.	Sodium Dodecyl Sulfate	195-198	colony stimulating factor 1	Homo sapiens	94-99	
8267882-4	1993	SDS-PAGE of this purified truncated M-CSF showed that its apparent molecular mass is 22 kDa under reducing conditions.	Sodium Dodecyl Sulfate	0-3	colony stimulating factor 1	Homo sapiens	36-41	
2029576-3	1991	A single band with a MW of 43 Kd, which reacted with anti-recombinant hM-CSF IgG but not with control IgG, was found when serum and urine from normal adults underwent electrophoresis on reduced sodium dodecyl sulfate-polyacrylamide gel and subsequent immunoblotting.	Sodium Dodecyl Sulfate	194-216	colony stimulating factor 1	Homo sapiens	70-76	
1880126-6	1991	SDS-PAGE of the purified truncated M-CSF gave a single band at 17 kDa under reducing conditions and at 32 kDa under non-reducing conditions.	Sodium Dodecyl Sulfate	0-3	colony stimulating factor 1	Homo sapiens	35-40