PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24261936-4 2013 We successfully designed suitable genetic circuits for expression of the URA3 selection marker gene to permit isolation of a- and alpha-type of cells, respectively, on solid medium lacking uracil. Uracil 189-195 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 73-77 23486708-3 2013 A proprietary vector was constructed containing a near-full-length HIV-1 genome with the yeast uracil biosynthesis (URA3) gene replacing the HIV-1 env coding sequence. Uracil 95-101 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 116-120 11020325-4 2000 The assay for insertion/deletion mutations uses a variety of different simple repeats placed in frame with URA3 such that insertions or deletions lead to a selectable Ura(-) phenotype; essentially all such mutations are in the simple repeat sequence. Uracil 167-170 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 107-111 19270272-4 2009 Changes in Pol I transcription at this gene alter the expression of an adjacent, modified URA3 reporter cassette (mURA3) such that reductions in Pol I transcription induce growth on synthetic media lacking uracil. Uracil 206-212 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 90-94 19026694-2 2009 To improve the expression of P. falciparum proteins in the yeast Saccharomyces cerevisiae, we selected yeast ura3 mutants that acquired the ability to utilize the P. falciparum orthologue (PfOMPDC) of URA3 to grow on media lacking uracil. Uracil 231-237 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 109-113 18780747-5 2008 This growth condition alters the epigenetics of rDNA silencing, such that repression of a URA3 reporter gene located at the rDNA induces growth on media that either lacks uracil or contains 5-fluoroorotic acid (5-FOA), an unusual dual phenotype that is reminiscent of telomeric silencing (TPE) of URA3. Uracil 171-177 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 90-94 17042755-2 2006 First, the OmURA3 gene encoding an orotidine-5"-phosphate decarboxylase was cloned and disrupted to generate a host strain with a uracil auxotrophic marker. Uracil 130-136 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 35-71 11297736-1 2001 Upon uracil depletion, the transcriptional activator Ppr1p stimulates expression of the Saccharomyces cerevisiae URA3 gene only four-fold. Uracil 5-11 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 113-117 10654087-8 1999 In two ura3 laboratory strains where selection for URA3 was applied independently of that for sulfite, the transformation efficiency for sulfite resistance was about 50% that for uracil prototrophy. Uracil 179-185 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 7-11 10654087-8 1999 In two ura3 laboratory strains where selection for URA3 was applied independently of that for sulfite, the transformation efficiency for sulfite resistance was about 50% that for uracil prototrophy. Uracil 179-185 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 51-55 9972254-2 1998 Using conventional genetic techniques, four mutant genes concerned in uracil biosynthesis were identified and denoted as ura1, ura2, ura3, and ura4. Uracil 70-76 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 133-137 10436009-4 1999 By using a modified Ura3p as the reporter that is released from Cub, the local concentration between Sec63-Cub-RUra3p and the different Nub-constructs could be translated into the growth rate of yeast cells on media lacking uracil. Uracil 224-230 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 20-25 9829833-8 1998 Thus, the growth inhibition by leflunomide seen in a S. cerevisiae ura3 auxotroph is due to the inhibition of the entry of exogenous uracil via the Fur4 uracil permease. Uracil 133-139 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 67-71 9311863-6 1997 Similarly, a plasmid encoding B3URA3, an RNA3 derivative with the yeast URA3 gene replacing the coat gene, conferred uracil-independent growth to ura3- yeast only after 1a and 2a expression and galactose induction. Uracil 117-123 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 32-36 9311863-6 1997 Similarly, a plasmid encoding B3URA3, an RNA3 derivative with the yeast URA3 gene replacing the coat gene, conferred uracil-independent growth to ura3- yeast only after 1a and 2a expression and galactose induction. Uracil 117-123 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 146-150 9299778-2 1997 Uracil auxotrophs of S. alluvius were obtained by ethyl methanesulphonate mutagenesis and complemented using the ura3 gene from S. cerevisiae. Uracil 0-6 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 113-117 8978095-1 1996 An uracil auxotrophic mutant of baker"s yeast Torulaspora delbrueckii, which is resistant to 5-fluoro-orotic acid, was complemented by transformation with YEp24 which harbors 2 microns origin and URA3 derived from Saccharomyces cerevisiae. Uracil 3-9 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 196-200 7788715-4 1995 A uracil auxotrophic mutant derived from T. pretoriensis was transformed with three plasmids (YEp24, YRpHI, and YCp50) carrying the URA3 gene of S. cerevisiae by the lithium acetate method. Uracil 2-8 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 132-136 7811063-3 1994 Ura- auxotrophs were obtained by selecting for resistance to 5-fluoroorotic acid and were identified as ura3 mutants by transformation with P. stipitis URA3. Uracil 0-3 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 104-108 7811063-3 1994 Ura- auxotrophs were obtained by selecting for resistance to 5-fluoroorotic acid and were identified as ura3 mutants by transformation with P. stipitis URA3. Uracil 0-3 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 152-156 35612987-3 2022 A uracil-auxotrophic marker (URA3) recycling system developed in ascomycetous yeasts and fungi was applied to the P. antarctica Paura3 strain. Uracil 2-8 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 29-33 8358827-2 1993 In order to obtain this efficiency, we isolated ura- mutants cells which can be complemented by the URA3 gene from Saccharomyces cerevisiae. Uracil 48-51 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 100-104 8319296-5 1993 Plasmids isolated from the transformants (URA+TRP+) carry both unique sites (ApaI and NcoI) within the URA3 gene indicating the precise restitution of the 169-bp gap. Uracil 42-45 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 103-107 2204810-1 1990 Expression of the yeast pyrimidine biosynthetic gene, URA3, is induced three- to fivefold in response to uracil starvation, and this regulation is mediated by the transcriptional activator PPR1 (pyrimidine pathway regulator 1). Uracil 105-111 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 54-58 2165219-4 1990 As expected, the wild-type URA3 gene in mitochondria failed to complement the uracil auxotrophy (Ura-) caused by a nuclear ura3 mutation. Uracil 97-100 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 27-31 35567483-4 2022 To test this prediction, we engineered switcher and non-switcher yeast strains, in which the uracil biosynthesis gene URA3 is either continually expressed or switched on and off at two different rates (slow and fast switchers). Uracil 93-99 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 118-122 32993167-5 2020 We transformed a PCR product of the Saccharomyces cerevisiae URA3 gene (ScURA3) into an uracil auxotroph K. marxianus otherwise wildtype strain and picked 24 stable Ura+ transformants for sequencing analysis. Uracil 88-94 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 61-65 3031459-7 1987 The A81-Pu transformants heterozygous for the ura3 mutations were rendered homozygous and Ura- by UV-induced recombination. Uracil 90-93 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 46-50 6787605-6 1981 One of the fusions to the URA3 gene itself has been shown to place the expression of beta-galactosidase activity under uracil regulation in yeasts. Uracil 119-125 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 26-30 33750818-8 2021 Furthermore, since URA3 can also serve as a marker for uracil autotrophy, we propose that our approach is a valuable addition to any large-scale Y2H screen. Uracil 55-61 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 19-23 32993167-5 2020 We transformed a PCR product of the Saccharomyces cerevisiae URA3 gene (ScURA3) into an uracil auxotroph K. marxianus otherwise wildtype strain and picked 24 stable Ura+ transformants for sequencing analysis. Uracil 165-168 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 61-65 29459692-11 2018 Results show that the URA3 mutant will be a useful tool for screening Symbiodinium transformants, both ex and in hospite, as survival in the absence of uracil is possible only upon successful introduction of URA3. Uracil 152-158 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 22-26 32154330-4 2019 Next, these plasmids are transformed into ssa1-4Delta yeast [expressing native Ssa1 from an uracil-based (URA3) plasmid] which are subsequently cured of the original yeast on 5-Fluroorotic Acid (5-FOA). Uracil 92-98 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 106-110 25406949-6 2014 The method is based on an established selection system where uracil auxotrophy of URA3-deleted yeast cells is rescued by an exogenously expressed reporter protein, comprised of a fusion between the essential URA3 gene and a degradation determinant (degron). Uracil 61-67 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 82-86 25406949-6 2014 The method is based on an established selection system where uracil auxotrophy of URA3-deleted yeast cells is rescued by an exogenously expressed reporter protein, comprised of a fusion between the essential URA3 gene and a degradation determinant (degron). Uracil 61-67 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 208-212 25406949-8 2014 As cell growth in uracil-deficient medium is proportional to the relative levels of Ura3, growth kinetics are entirely dependent on the reporter protein degradation. Uracil 18-24 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 84-88