PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 32904132-6 2020 Aflatoxin B1 (AFB1)-specific codon 249 TP53 mutation was determined by NGS in circulating cell-free plasma DNA. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 39-43 33457005-0 2020 Genome-wide association study of the TP53 R249S mutation in hepatocellular carcinoma with aflatoxin B1 exposure and infection with hepatitis B virus. Aflatoxin B1 90-102 tumor protein p53 Homo sapiens 37-41 33457005-1 2020 Background: Exposure to dietary aflatoxin B1 (AFB1) induces DNA damage and mutation in the TP53 gene at codon 249, known as the TP53 R249S mutation, and is a major risk factor for hepatocellular carcinoma (HCC). Aflatoxin B1 32-44 tumor protein p53 Homo sapiens 91-95 33457005-1 2020 Background: Exposure to dietary aflatoxin B1 (AFB1) induces DNA damage and mutation in the TP53 gene at codon 249, known as the TP53 R249S mutation, and is a major risk factor for hepatocellular carcinoma (HCC). Aflatoxin B1 32-44 tumor protein p53 Homo sapiens 128-132 33457005-1 2020 Background: Exposure to dietary aflatoxin B1 (AFB1) induces DNA damage and mutation in the TP53 gene at codon 249, known as the TP53 R249S mutation, and is a major risk factor for hepatocellular carcinoma (HCC). Aflatoxin B1 46-50 tumor protein p53 Homo sapiens 91-95 33457005-1 2020 Background: Exposure to dietary aflatoxin B1 (AFB1) induces DNA damage and mutation in the TP53 gene at codon 249, known as the TP53 R249S mutation, and is a major risk factor for hepatocellular carcinoma (HCC). Aflatoxin B1 46-50 tumor protein p53 Homo sapiens 128-132 32904132-6 2020 Aflatoxin B1 (AFB1)-specific codon 249 TP53 mutation was determined by NGS in circulating cell-free plasma DNA. Aflatoxin B1 14-18 tumor protein p53 Homo sapiens 39-43 29225033-2 2017 Here we report a unique mechanism underlying the GOF of p53-R249S (p53-RS), a p53 mutant frequently detected in human hepatocellular carcinoma (HCC) that is highly related to hepatitis B infection and aflatoxin B1. Aflatoxin B1 201-213 tumor protein p53 Homo sapiens 56-59 31747859-1 2020 p53-R249S (p53-RS) is frequently detected in human hepatocellular carcinoma (HCC) that is highly associated with hepatitis B infection and aflatoxin B1 exposure. Aflatoxin B1 139-151 tumor protein p53 Homo sapiens 0-3 31747859-1 2020 p53-R249S (p53-RS) is frequently detected in human hepatocellular carcinoma (HCC) that is highly associated with hepatitis B infection and aflatoxin B1 exposure. Aflatoxin B1 139-151 tumor protein p53 Homo sapiens 11-14 30608603-3 2019 p53-R249S is the sole hotspot mutation in hepatocellular carcinoma (HCC) that is highly associated with chronic hepatitis B virus (HBV) infection and dietary exposure to aflatoxin B1 (AFB1). Aflatoxin B1 170-182 tumor protein p53 Homo sapiens 0-3 30608603-3 2019 p53-R249S is the sole hotspot mutation in hepatocellular carcinoma (HCC) that is highly associated with chronic hepatitis B virus (HBV) infection and dietary exposure to aflatoxin B1 (AFB1). Aflatoxin B1 184-188 tumor protein p53 Homo sapiens 0-3 29750510-3 2018 Bioactivated aflatoxin B1 was reacted with a 32 bp exon 7 fragment of the p53 gene using eight microsomal cytochrome (cyt) P450 enzymes from different organs coated on magnetic beads. Aflatoxin B1 13-25 tumor protein p53 Homo sapiens 74-77 29750510-5 2018 This is the first demonstration in a cell-free medium that the aflatoxin B1 metabolite selectively causes abasic site formation and strand breaks at codon 249 of the p53 probe, corresponding to the chemical pathway and mutations of p53 in human liver cells and tumors. Aflatoxin B1 63-75 tumor protein p53 Homo sapiens 166-169 29750510-5 2018 This is the first demonstration in a cell-free medium that the aflatoxin B1 metabolite selectively causes abasic site formation and strand breaks at codon 249 of the p53 probe, corresponding to the chemical pathway and mutations of p53 in human liver cells and tumors. Aflatoxin B1 63-75 tumor protein p53 Homo sapiens 232-235 29225033-2 2017 Here we report a unique mechanism underlying the GOF of p53-R249S (p53-RS), a p53 mutant frequently detected in human hepatocellular carcinoma (HCC) that is highly related to hepatitis B infection and aflatoxin B1. Aflatoxin B1 201-213 tumor protein p53 Homo sapiens 67-70 29225033-2 2017 Here we report a unique mechanism underlying the GOF of p53-R249S (p53-RS), a p53 mutant frequently detected in human hepatocellular carcinoma (HCC) that is highly related to hepatitis B infection and aflatoxin B1. Aflatoxin B1 201-213 tumor protein p53 Homo sapiens 67-70 28882572-6 2017 In addition to the top candidate p53, we identified several other interesting TFs that modulated gamma-H2AX after BaP and AFB1 treatment. Aflatoxin B1 122-126 tumor protein p53 Homo sapiens 33-36 30123836-7 2017 This review article mainly summarizes extrinsic factors that induce liver cancer and potentially have etiological association with p53, including aflatoxin B1, vinyl chloride, non-alcoholic fatty liver disease, iron overload, and infection of hepatitis viruses. Aflatoxin B1 146-158 tumor protein p53 Homo sapiens 131-134 27750242-6 2016 For example, specific TP53 mutations frequently induced by aflatoxin B1 exposure might affect histone modifiers and nucleosome remodelers. Aflatoxin B1 59-71 tumor protein p53 Homo sapiens 22-26 24461059-2 2015 Chronic infection with hepatitis B virus (HBV) and exposure to aflatoxin B1 (AFB1) induces p53 mutations in hepatocellular carcinoma (HCC) tissue. Aflatoxin B1 63-75 tumor protein p53 Homo sapiens 91-94 24736102-10 2015 Finally, a R249S mutation of TP53, well-known hallmark of aflatoxin B1 exposure, was found. Aflatoxin B1 58-70 tumor protein p53 Homo sapiens 29-33 26738694-0 2016 Effect of p53 Arg72Pro polymorphism on the induction of micronucleus by aflatoxin B1 in in vitro in human blood lymphocytes. Aflatoxin B1 72-84 tumor protein p53 Homo sapiens 10-13 26738694-3 2016 The aim of this study is to investigate association between p53 Arg72Pro polymorphism and the frequencies of spontaneous and AFB1-induced DNA damage in peripheral blood lymphocytes from 100 healthy individuals in Turkish population. Aflatoxin B1 125-129 tumor protein p53 Homo sapiens 60-63 26738694-7 2016 Moreover, genotype analysis revealed a statistically significant association between Pro/Pro genotype of p53 Arg72Pro polymorphism and increased frequencies of MN both spontaneous and AFB1-induced cultures when compared Arg/Arg genotype (0.69 +- 0.19 versus 0.46 +- 0.13, p < 0.001; 1.59 +- 0.65 versus 1.01 +- 0.41 p < 0.001; respectively). Aflatoxin B1 184-188 tumor protein p53 Homo sapiens 105-108 26738694-8 2016 Our data indicate that p53 Arg72Pro polymorphism plays a significant role in human sensitivity to the genotoxic effects of AFB1. Aflatoxin B1 123-127 tumor protein p53 Homo sapiens 23-26 24461059-2 2015 Chronic infection with hepatitis B virus (HBV) and exposure to aflatoxin B1 (AFB1) induces p53 mutations in hepatocellular carcinoma (HCC) tissue. Aflatoxin B1 77-81 tumor protein p53 Homo sapiens 91-94 20655369-7 2010 While cyclophosphamide showed an elevation of activated P53 in the presence of S9, 7,12-dimethylbenz[a]anthracene and aflatoxin B(1) responded without the MAS. Aflatoxin B1 118-129 tumor protein p53 Homo sapiens 56-59 24460352-1 2013 BACKGROUND: A missense mutation in exon 7 (R249S) of the p53 tumor suppressor gene is characteristic of aflatoxin B1 (AFB1) exposure. Aflatoxin B1 104-116 tumor protein p53 Homo sapiens 57-60 24460352-1 2013 BACKGROUND: A missense mutation in exon 7 (R249S) of the p53 tumor suppressor gene is characteristic of aflatoxin B1 (AFB1) exposure. Aflatoxin B1 118-122 tumor protein p53 Homo sapiens 57-60 20963515-11 2011 The very low frequency of TP53 mutation suggests low levels of aflatoxin B1 exposure. Aflatoxin B1 63-75 tumor protein p53 Homo sapiens 26-30 22319594-6 2012 By using a functional assay, the Functional Analysis of Separated Alleles in Yeast (FASAY), the present study depicts the mutational pattern of TP53 in normal human fibroblasts after in vitro exposure to well-known carcinogens: benzo[a]pyrene, aflatoxin B(1) and acetaldehyde. Aflatoxin B1 244-255 tumor protein p53 Homo sapiens 144-148 20655369-8 2010 Inhibition of cellular CYP3A4 or CYP1A/1B suppressed the aflatoxin B(1)- and dimethylbenz[a]anthracene-mediated P53 response, respectively, indicating that HepG2 cells are capable of metabolizing these compounds in a CYP1A/B/3A4-dependent manner. Aflatoxin B1 57-68 tumor protein p53 Homo sapiens 112-115 19345001-3 2009 Aflatoxin B1, the most commonly occurring and potent of the aflatoxins is associated with a specific AGG to AGT transversion mutation at codon 249 of the p53 gene in human HCC, providing mechanistic support to a causal link between exposure and disease. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 154-157 16280384-0 2006 Aflatoxin B1 alters the expression of p53 in cytochrome P450-expressing human lung cells. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 38-41 19524575-0 2009 In vitro recapitulating of TP53 mutagenesis in hepatocellular carcinoma associated with dietary aflatoxin B1 exposure. Aflatoxin B1 96-108 tumor protein p53 Homo sapiens 27-31 19524575-9 2009 CONCLUSIONS: In this model system, AFB(1)-induced DNA adduction and mutagenesis recapitulate the unique mutational features of TP53 in AFB(1)-associated human HCC. Aflatoxin B1 35-41 tumor protein p53 Homo sapiens 127-131 19152081-3 2009 For the genetic changes closely related to etiology of liver cancer, the well-known cases include insertion and integration of the hepatitis B virus (HBV) DNA after infection, and mutations at site 249 of the tumor suppressor gene p53 induced by exposure to aflatoxin B1. Aflatoxin B1 258-270 tumor protein p53 Homo sapiens 231-234 18725321-0 2008 Aflatoxin B1-induced TP53 mutational pattern in normal human cells using the FASAY (Functional Analysis of Separated Alleles in Yeast). Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 21-25 17849423-1 2008 In hepatocellular carcinoma (HCC), hotspot mutation in codon 249 of the p53 gene has been associated with exposure to aflatoxin B1 (AFB1). Aflatoxin B1 118-130 tumor protein p53 Homo sapiens 72-75 16605112-3 2006 AAG to AGT transversion at codon 249 of the P53 gene arg-ser (249ser) has been identified as a hotspot, reflecting DNA damage caused by aflatoxin B1 metabolites in HCC. Aflatoxin B1 136-148 tumor protein p53 Homo sapiens 44-47 18358501-7 2008 Interestingly, 9 out of 14 (64.3%) tumors carrying p53 mutations displayed substitution of serine by arginine at codon 249, a characteristic change believed to be induced by aflatoxin-B1. Aflatoxin B1 174-186 tumor protein p53 Homo sapiens 51-54 16557586-0 2006 Aflatoxin-B exposure does not lead to p53 mutations but results in enhanced liver cancer of Hupki (human p53 knock-in) mice. Aflatoxin B1 0-11 tumor protein p53 Homo sapiens 105-108 16410370-7 2006 Aflatoxin B1, hepatitis B virus, hepatitis C virus, and vinyl chloride all caused TP53 mutations in human liver tumors, but the mutation spectrum for each agent differed. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 82-86 16280384-2 2006 Mutations (and altered expression) of the tumor suppresser gene p53 have been observed in liver tumors from patients exposed to high dietary AFB1. Aflatoxin B1 141-145 tumor protein p53 Homo sapiens 64-67 16280384-4 2006 We examined the effects of low concentrations of AFB1 on the expression of p53 and MDM2 in human bronchial epithelial cells (BEAS-2B) transfected with cDNA for either cytochrome P450 (CYP) 1A2 (B-CMV1A2) or CYP 3A4 (B3A4), two isozymes that are responsible for AFB1 activation in human liver and possibly the lung. Aflatoxin B1 49-53 tumor protein p53 Homo sapiens 75-78 16048565-1 2005 BACKGROUND AND AIMS: A specific mutation at codon 249 of the p53 tumor suppressor gene (guanine to thymine; arginine to serine [249(serine)p53]) is present in the cell-free plasma of 30-47% of patients with hepatocellular carcinoma (HCC) in regions with uniformly high levels of dietary exposure to the fungal toxin, aflatoxin B(1). Aflatoxin B1 317-328 tumor protein p53 Homo sapiens 61-64 17113242-1 2006 INTRODUCTION: The most frequent mutation in human hepatocellular carcinoma (HCC) in populations exposed to a high dietary intake of aflatoxin B1 (AFB1) is a mutation in codon 249 of the p53 gene. Aflatoxin B1 132-144 tumor protein p53 Homo sapiens 186-189 17113242-1 2006 INTRODUCTION: The most frequent mutation in human hepatocellular carcinoma (HCC) in populations exposed to a high dietary intake of aflatoxin B1 (AFB1) is a mutation in codon 249 of the p53 gene. Aflatoxin B1 146-150 tumor protein p53 Homo sapiens 186-189 17113242-3 2006 We hypothesized that the combination of schistosomiasis and aflatoxin B1 increases the incidence of p53 gene mutation. Aflatoxin B1 60-72 tumor protein p53 Homo sapiens 100-103 17113242-8 2006 CONCLUSION: These data suggest that schistosomiasis and exposure to aflatoxin B1 act synergistically to increase the incidence of p53 gene mutation. Aflatoxin B1 68-80 tumor protein p53 Homo sapiens 130-133 16365016-1 2005 A mutation in codon 249 of the TP53 gene (249(Ser)), related to aflatoxin B(1) exposure, has previously been associated with hepatocellular carcinoma risk. Aflatoxin B1 64-75 tumor protein p53 Homo sapiens 31-35 16048565-1 2005 BACKGROUND AND AIMS: A specific mutation at codon 249 of the p53 tumor suppressor gene (guanine to thymine; arginine to serine [249(serine)p53]) is present in the cell-free plasma of 30-47% of patients with hepatocellular carcinoma (HCC) in regions with uniformly high levels of dietary exposure to the fungal toxin, aflatoxin B(1). Aflatoxin B1 317-328 tumor protein p53 Homo sapiens 139-142 16048565-9 2005 CONCLUSIONS: The 249(serine)p53 mutation is found less often in the serum of patients with HCC in a region with variable levels of exposure to aflatoxin B(1) than in those with uniformly high levels of exposure, but the mutation does occur in black Africans with presumed lower levels of exposure to the fungal toxin. Aflatoxin B1 143-154 tumor protein p53 Homo sapiens 28-31 12963117-0 2003 In vitro aflatoxin B1-induced p53 mutations. Aflatoxin B1 9-21 tumor protein p53 Homo sapiens 30-33 15534906-1 2004 AIM: To investigate p53 mutation and p21 expression in hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B(1) (AFB(1)) in tree shrews, and to reveal the role of these genes in hepatocarcinogenesis. Aflatoxin B1 115-126 tumor protein p53 Homo sapiens 20-23 12869416-1 2003 A specific missense mutation in the p53 tumor gene at codon 249 has been reported in over 50% of hepatocellular carcinoma (HCC) tumors and in paired blood samples from areas of high dietary exposure to aflatoxin B1, including Qidong, People"s Republic of China. Aflatoxin B1 202-214 tumor protein p53 Homo sapiens 36-39 12845670-2 2003 While the G-->T p53 mutation at codon 249 has been identified as a genetic hallmark of HCC caused by aflatoxin B(1), the genetic profile associated with other etiologic factors appears to be less distinctive. Aflatoxin B1 104-115 tumor protein p53 Homo sapiens 19-22 12619109-3 2003 In populations exposed to dietary aflatoxin B1 with liver cancer (AFB1) and ultraviolet (UV)-radiation with skin cancer, a single specific-looking TP53 mutation has been described in some of the tumors. Aflatoxin B1 34-46 tumor protein p53 Homo sapiens 147-151 12376521-2 2002 A specific missense mutation resulting from a guanine to thymine transversion at the third position of codon 249 in the p53 tumor suppressor gene has been reported in 10-70% of HCCs from areas of high dietary exposure to aflatoxin B(1.) Aflatoxin B1 221-232 tumor protein p53 Homo sapiens 120-123 12681064-1 2003 OBJECTIVE: To detect the expression and variation of p53 gene during tree shrews" hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1). Aflatoxin B1 142-154 tumor protein p53 Homo sapiens 53-56 12046074-1 2002 AIM: In hepatocellular carcinoma (HCC) prevalent areas of China, the point mutation of p53 exon7 is highly correlated with Hepatitis B virus(HBV) infection and aflatoxin B intake. Aflatoxin B1 160-171 tumor protein p53 Homo sapiens 87-90 12011430-1 2002 A G to T mutation has been observed at the third position of codon 249 of the p53 tumor-suppressor gene in over 50% of the hepatocellular carcinoma cases associated with high exposure to aflatoxin B(1) (AFB(1)). Aflatoxin B1 187-198 tumor protein p53 Homo sapiens 78-81 10856831-2 2000 AGG to AGT transversion in codon 249 of exon 7 of the p53 gene occurs in over 50% of HCC from endemic regions, where both chronic infection with the hepatitis B virus (HBV) and exposure to carcinogens such as aflatoxin B1 (AFB1) prevail. Aflatoxin B1 209-221 tumor protein p53 Homo sapiens 54-57 12462448-5 2002 Observations that mutations in gene p53 appear under conditions of occupational and environmental exposures to chemical and physical carcinogens, such as vinyl chloride, radon, or aflatoxin B1, have proved to be of enormous importance for the occupational and environmental health. Aflatoxin B1 180-192 tumor protein p53 Homo sapiens 36-39 11526514-8 2001 Our data revealed a relationship between chromosome 16q homozygous deletions and R249S p53 mutations in tumors where the patient had been exposed to aflatoxin B1 (P=0.002). Aflatoxin B1 149-161 tumor protein p53 Homo sapiens 87-90 11196182-2 2001 A specific missense mutation resulting from a guanine to thymine transversion at the third position of codon 249 in the p53 tumor suppressor gene has been reported in 10-70% of HCCs from areas of high dietary exposure to aflatoxin B1. Aflatoxin B1 221-233 tumor protein p53 Homo sapiens 120-123 11126384-4 2000 Aflatoxin B1 exposure leads to mutations in the p53 tumor suppressor gene, most commonly a transversion in codon 249 that leads to a substitution of serine for arginine in the p53 protein. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 48-51 11126384-4 2000 Aflatoxin B1 exposure leads to mutations in the p53 tumor suppressor gene, most commonly a transversion in codon 249 that leads to a substitution of serine for arginine in the p53 protein. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 176-179 10949925-0 2000 Activation of the insulin-like growth factor II transcription by aflatoxin B1 induced p53 mutant 249 is caused by activation of transcription complexes; implications for a gain-of-function during the formation of hepatocellular carcinoma. Aflatoxin B1 65-77 tumor protein p53 Homo sapiens 86-89 10949925-1 2000 Aflatoxin B1 (AFB1) induced mutation of the p53 gene at codon 249 (p53mt249) is critical during the formation of hepatocellular carcinoma (HCC) following hepatitis B virus (HBV) infection. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 44-47 10738214-1 2000 BACKGROUND: Specific mutations of the p53 tumor suppressor gene in hepatocellular carcinoma (HCC) have been reported from several parts of the world, but to the authors" knowledge to date the status of this gene has not been studied in HCC patients in India, where HCC is one of the major cancers and the frequency of chronic hepatitis B virus (HBV) as well as hepatitis C virus (HCV) infection and exposure to dietary aflatoxin B(1) is very high. Aflatoxin B1 419-430 tumor protein p53 Homo sapiens 38-41 10863098-0 2000 Mutations in the p53 tumor suppressor gene in tree shrew hepatocellular carcinoma associated with hepatitis B virus infection and intake of aflatoxin B1. Aflatoxin B1 140-152 tumor protein p53 Homo sapiens 17-20 10458704-0 1999 Site-specific synthesis of aflatoxin B(1) adducts within an oligodeoxyribonucleotide containing the human p53 codon 249 sequence. Aflatoxin B1 27-38 tumor protein p53 Homo sapiens 106-109 10767641-5 2000 Characteristic p53 mutation spectra have been associated with dietary aflatoxin B(1) (AFB(1)) exposure and hepatocellular carcinoma (HCC); sunlight exposure and skin cancer; and cigarette smoking and lung cancer. Aflatoxin B1 70-84 tumor protein p53 Homo sapiens 15-18 10767641-5 2000 Characteristic p53 mutation spectra have been associated with dietary aflatoxin B(1) (AFB(1)) exposure and hepatocellular carcinoma (HCC); sunlight exposure and skin cancer; and cigarette smoking and lung cancer. Aflatoxin B1 86-92 tumor protein p53 Homo sapiens 15-18 10767646-6 2000 This indicates that p53 mutation is associated with progression, rather than early development, of HCC in the low-aflatoxin B(1)-exposed region. Aflatoxin B1 114-128 tumor protein p53 Homo sapiens 20-23 10592324-1 1999 Mutations in the TP53 tumor suppressor gene are the most common alteration in cancer, and human primary liver cancers related to previous dietary exposure to the mycotoxin aflatoxin B1 (AFB1) exhibit a specific hot spot mutation at TP53 codon 249. Aflatoxin B1 172-184 tumor protein p53 Homo sapiens 17-21 10592324-1 1999 Mutations in the TP53 tumor suppressor gene are the most common alteration in cancer, and human primary liver cancers related to previous dietary exposure to the mycotoxin aflatoxin B1 (AFB1) exhibit a specific hot spot mutation at TP53 codon 249. Aflatoxin B1 172-184 tumor protein p53 Homo sapiens 232-236 10592324-6 1999 The genotoxic action of AFB1 was completely different from that of the alkylating agent ethyl-methane-sulfonate, where 28/30 induced mutations were linked to the TP53 target gene. Aflatoxin B1 24-28 tumor protein p53 Homo sapiens 162-166 10612830-4 2000 For example, the p53 mutational spectrum reveals evidence for a direct causal effect of ultraviolet radiation in skin cancer, of aflatoxin B1 in liver cancer, and of tobacco smoke in lung cancer. Aflatoxin B1 129-141 tumor protein p53 Homo sapiens 17-20 10389750-2 1999 The relation between aflatoxin B1 (AFB1 )-related hepatocellular carcinomas (HCCs) and hot spot at codon 249 of the p53 gene has received a great deal of attention, but its significance is still controversial. Aflatoxin B1 21-33 tumor protein p53 Homo sapiens 116-119 10389750-2 1999 The relation between aflatoxin B1 (AFB1 )-related hepatocellular carcinomas (HCCs) and hot spot at codon 249 of the p53 gene has received a great deal of attention, but its significance is still controversial. Aflatoxin B1 35-39 tumor protein p53 Homo sapiens 116-119 10381922-2 1999 Aflatoxin B1 causes a specific point mutation in the p53 tumor-suppressor gene in exposed individuals. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 53-56 10517975-8 1999 For example, exposure to ultraviolet light is correlated with transition mutations at dipyrimidine sites; aflatoxin B(1) exposure is correlated with a G:C to T:A transversion that leads to a serine substitution at residue 249 of p53 in hepatocellular carcinoma; and exposure to cigarette smoke is correlated with G:C to T:A transversions in lung carcinoma. Aflatoxin B1 106-117 tumor protein p53 Homo sapiens 229-232 10051487-1 1999 A particular point mutation of the tumor suppressor gene p53, namely a G-->T transversion at the third base of codon 249, is frequently detected in primary hepatocellular carcinomas from patients living in areas where the levels of dietary exposure to aflatoxin B1 and the rates of infection with the hepatitis B virus are very high. Aflatoxin B1 255-267 tumor protein p53 Homo sapiens 57-60 12953991-0 1999 P53 gene of chang-liver cells (Atcc-Ccl13) exposed to aflatoxin B1 (Afb): the effect of lysine on mutation at codon 249 of exon 7. Aflatoxin B1 54-66 tumor protein p53 Homo sapiens 0-3 12953991-0 1999 P53 gene of chang-liver cells (Atcc-Ccl13) exposed to aflatoxin B1 (Afb): the effect of lysine on mutation at codon 249 of exon 7. Aflatoxin B1 68-71 tumor protein p53 Homo sapiens 0-3 12953991-1 1999 The effect of different regimes of lysine-pre treatment on mutation at the 3rd nucleotide base of codon 249 which is located at the 7th exon of p53 gene of Chang-liver cells (CCIL13) exposed to aflatoxin B1 (AFB1) has been investigated. Aflatoxin B1 194-206 tumor protein p53 Homo sapiens 144-147 10394883-2 1999 The incidence of p53 gene abnormalities in human hepatocellular carcinoma (HCC) varies in different geographical areas, being higher in regions where hepatitis virus infection and dietary exposure to aflatoxin B1 are the most common aetiological agents. Aflatoxin B1 200-212 tumor protein p53 Homo sapiens 17-20 10374839-3 1999 In vitro experiments using human cell line cells and aflatoxin B1 have demonstrated the induction of p53 mutations in codon 249 and adjacent codons. Aflatoxin B1 53-65 tumor protein p53 Homo sapiens 101-104 10374839-6 1999 In an in vivo rodent model systems using the aflatoxin B1-sensitive male F344 rat, previous studies have shown that hepatocarcinogenesis is accompanied by significant incidences of codon 12 mutations in K-ras and codon 13 mutations in N-ras genes, but in contrast to the human, apparently not by mutations in codon 243 of the p53 gene (which corresponds to codon 249 in the human gene). Aflatoxin B1 45-57 tumor protein p53 Homo sapiens 326-329 10051487-5 1999 Thus, these results strongly support the view that the mutation at codon 249 of the p53 gene may serve as a fingerprint for aflatoxin B1-induced hepatocellular carcinomas, but is not, by itself, sufficient to immortalize human liver cells. Aflatoxin B1 124-136 tumor protein p53 Homo sapiens 84-87 11819223-1 1998 AIM:To study the p53 gene mutation and its relationship to aflatoxin B1 exposure in hepatocellular carcinoma (HCC).METHODS: Restriction fragment length polymorphism analysis method was used in 62 HCC samples, and DNA direct sequencing in another 45 HCC samples.RESULTS: In HCC and AFB1 high and low-risk areas, 36/52 (69%) and 2/10 (20%) cases were found losing the HaeIII allele respectively, suggesting one of the base G mutation at the p53 gene codon 249. Aflatoxin B1 59-71 tumor protein p53 Homo sapiens 17-20 9667752-4 1998 N-Methyl-N"-nitro-nitrosoguanidine and N-ethyl-N-nitrosourea, two direct-acting genotoxic (DNA-reactive) carcinogens, caused p53 induction as early as 2 h following treatment, with peak increases within 4-12 h. Aflatoxin B1 and 2-acetylaminofluorene, indirect-acting genotoxic carcinogens, caused a later induction of p53, with the peak increase appearing between 16 and 24 h following treatment. Aflatoxin B1 211-223 tumor protein p53 Homo sapiens 125-128 10022257-8 1998 For example, characteristic p53 mutation spectra have been associated with: dietary aflatoxin B1 exposure and hepatocellular carcinoma; sunlight exposure and skin carcinoma; and cigarette smoking and lung cancer. Aflatoxin B1 84-96 tumor protein p53 Homo sapiens 28-31 9570360-2 1997 The most frequent p53 mutation has been found in HCCs in regions with high hepatitis B virus (HBV) infection and intake of aflatoxin B1 (AFB1). Aflatoxin B1 123-135 tumor protein p53 Homo sapiens 18-21 10921048-11 1998 The close association of the hotspot mutation of p53 gene in Qidong HCC with the presence of HBVx gene sequence suggests that such mutation is the molecular footprint of the combined effect of aflatoxin B1 exposure and HBVx gene product. Aflatoxin B1 193-205 tumor protein p53 Homo sapiens 49-52 10026991-8 1998 For example, characteristic p53 mutation spectra have been associated with: dietary aflatoxin B1 exposure and hepatocellular carcinoma; sunlight exposure and skin carcinoma; and cigarette smoking and lung cancer. Aflatoxin B1 84-96 tumor protein p53 Homo sapiens 28-31 9230270-1 1997 Epidemiological evidence has been supporting a relationship between dietary aflatoxin B1 (AFB1) exposure, development of human primary hepatocellular carcinoma (HCC) and mutations in the p53 tumor suppressor gene. Aflatoxin B1 76-88 tumor protein p53 Homo sapiens 187-190 9230270-1 1997 Epidemiological evidence has been supporting a relationship between dietary aflatoxin B1 (AFB1) exposure, development of human primary hepatocellular carcinoma (HCC) and mutations in the p53 tumor suppressor gene. Aflatoxin B1 90-94 tumor protein p53 Homo sapiens 187-190 8796849-3 1996 For example, the p53 mutational spectrum reveals evidence for a direct causal effect of ultraviolet radiation in skin cancer, of aflatoxin B1 in liver cancer and of tobacco smoke in lung cancer. Aflatoxin B1 129-141 tumor protein p53 Homo sapiens 17-20 9006107-0 1996 Absence of mutations in the p53 tumor suppressor gene in woodchuck hepatocellular carcinomas associated with hepadnavirus infection and intake of aflatoxin B1. Aflatoxin B1 146-158 tumor protein p53 Homo sapiens 28-31 8640905-2 1996 The most frequent mutation in HCC in populations exposed to a high dietary intake of aflatoxin B1 (AFB1) is an AGGarg-->AGTser missense mutation in codon 249 of the p53 gene. Aflatoxin B1 85-97 tumor protein p53 Homo sapiens 168-171 8779543-1 1996 Mutations of the p53 tumour-suppressor gene in human hepatocellular carcinomas from certain geographic areas appear to be associated with high dietary exposure to aflatoxin B1 (AFB1). Aflatoxin B1 163-175 tumor protein p53 Homo sapiens 17-20 8779543-1 1996 Mutations of the p53 tumour-suppressor gene in human hepatocellular carcinomas from certain geographic areas appear to be associated with high dietary exposure to aflatoxin B1 (AFB1). Aflatoxin B1 177-181 tumor protein p53 Homo sapiens 17-20 8895534-1 1996 AGG to AGT mutations in codon 249 of the p53 tumor-suppressor gene are frequently observed in hepatocellular carcinomas (HCC) from areas where exposure to aflatoxin B1 (AFB) occurs. Aflatoxin B1 155-167 tumor protein p53 Homo sapiens 41-44 8895534-1 1996 AGG to AGT mutations in codon 249 of the p53 tumor-suppressor gene are frequently observed in hepatocellular carcinomas (HCC) from areas where exposure to aflatoxin B1 (AFB) occurs. Aflatoxin B1 169-172 tumor protein p53 Homo sapiens 41-44 8580407-3 1995 Six ducks with HCC, five of which were fed a diet containing aflatoxin B1 for 1-2 years, were analysed for the presence of point mutations at this codon of the p53 gene by polymerase chain reaction and direct nucleotide sequencing. Aflatoxin B1 61-73 tumor protein p53 Homo sapiens 160-163 7892276-1 1995 Aflatoxin B1 (AFB1) has been postulated to be a hepatocarcinogen in humans, possibly by causing p53 mutations at codon 249. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 96-99 7907948-6 1994 In particular, we studied the mutability of codons 247-250 of p53 with the mycotoxin aflatoxin B1 (AFB1) in human hepatocytes. Aflatoxin B1 85-97 tumor protein p53 Homo sapiens 62-65 8036011-7 1994 It is evident that H2O2/FeCl3 possesses essentially the same mutagenic specificity for codons 249 and 250 of p53 as bulky carcinogens such as aflatoxin B1, benzo(a)pyrene or heterocyclic amines. Aflatoxin B1 142-154 tumor protein p53 Homo sapiens 109-112 7713039-3 1994 This hypothesis has been supported by genetic evidence in liver tumors which has associated aflatoxin B1 exposure with the detection of inactivating DNA mutations within the human p53 tumor suppressor gene. Aflatoxin B1 92-104 tumor protein p53 Homo sapiens 180-183 7816989-2 1994 The mutation of an allele on the p53 gene with loss of the healthy allele, in different tissues such as lung, larynx, bladder, liver, skin, colon and breast, which may or may not be exposed to chemical or physical carcinogens (tobacco, radon, ultraviolet, aflatoxin B1), is associated with the occurrence of cancer. Aflatoxin B1 256-268 tumor protein p53 Homo sapiens 33-36 7903205-1 1994 Aflatoxin B1 has been suggested as a causative agent for a G to T mutation at codon 249 in the p53 gene in human hepatocellular carcinomas (HCC) from southern Africa and Qidong in China. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 95-98 1330291-1 1992 In order to clarify the significance of mutation of the p53 tumor suppressor gene in the genesis and development of human hepatocellular carcinoma (HCC) in an aflatoxin B1 low-exposure area, the spectrum, i.e., incidence, type, and site, of p53 gene mutations was examined in 169 tissue samples resected mainly from Japanese patients using single-strand conformation polymorphism analysis and direct sequencing. Aflatoxin B1 159-171 tumor protein p53 Homo sapiens 56-59 8397412-0 1993 Aflatoxin B1 induces the transversion of G-->T in codon 249 of the p53 tumor suppressor gene in human hepatocytes. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 70-73 8301066-8 1993 Although many HCC patients displaying P53 mutations also suffer from HBV infection, which itself can lead to rearrangements of P53 coding regions or induce the synthesis of viral proteins possibly interacting with p53, the specific G to T transversion within codon 249 of the P53 gene seems to directly reflect the extent of aflatoxin B1 exposure. Aflatoxin B1 325-337 tumor protein p53 Homo sapiens 214-217 8389256-1 1993 A G:C-->T:A mutational hotspot at codon 249 of the p53 tumor suppressor gene has previously been identified in hepatocellular carcinoma (HCC) of patients from Qidong, China and southern Africa in which aflatoxin B1 (AFB1) and hepatitis B virus (HBV) are known synergistic risk factors. Aflatoxin B1 205-217 tumor protein p53 Homo sapiens 54-57 8096551-2 1993 Most of the mutations were G to T transversions in codon 249 of the p53 gene in HCCs in China and Mozambique, where aflatoxin B1 was a risk factor. Aflatoxin B1 116-128 tumor protein p53 Homo sapiens 68-71 8301066-8 1993 Although many HCC patients displaying P53 mutations also suffer from HBV infection, which itself can lead to rearrangements of P53 coding regions or induce the synthesis of viral proteins possibly interacting with p53, the specific G to T transversion within codon 249 of the P53 gene seems to directly reflect the extent of aflatoxin B1 exposure. Aflatoxin B1 325-337 tumor protein p53 Homo sapiens 38-41 1324739-7 1992 A mutational "hotspot" in the p53 gene in human hepatocellular carcinomas has been identified that could reflect exposure to a specific carcinogen, one candidate being aflatoxin B1. Aflatoxin B1 168-180 tumor protein p53 Homo sapiens 30-33 1310637-0 1992 Low frequency of p53 gene mutation in tumors induced by aflatoxin B1 in nonhuman primates. Aflatoxin B1 56-68 tumor protein p53 Homo sapiens 17-20 1310637-1 1992 Aflatoxin B1 has been suggested as a causative agent for a G to T mutation at codon 249 in the p53 gene in human hepatocellular carcinomas from southern Africa and Qidong in China. Aflatoxin B1 0-12 tumor protein p53 Homo sapiens 95-98 1310637-7 1992 The occurrence of mutation in codon 249 of the p53 gene in selective samples of human hepatocellular cancers may indicate involvement of environmental carcinogens other than aflatoxin B1 or that hepatitis B virus-related hepatitis is a prerequisite for aflatoxin B1 induction of G to T transversion in codon 249. Aflatoxin B1 174-186 tumor protein p53 Homo sapiens 47-50 1332185-3 1992 Recent reports of a guanine to thymine mutation of the third base of codon 249 of the tumour suppressor gene, p53, in 50% of patients with hepatocellular carcinoma in regions of high aflatoxin exposure, and mutagenic experiments showing that aflatoxin B1 binds particularly to guanine residues in G-C-rich domains and that codon 249 is a preferred target have suggested a mechanism whereby aflatoxin might induce malignant transformation. Aflatoxin B1 242-254 tumor protein p53 Homo sapiens 110-113