PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10852917-5 2000 Values for K(m) and V(max) were determined to be 12.2 nm and 250.7 fmol/min, respectively, at 0 degrees C. The calculated turnover number (K(cat)) of 43.2 pmol of GDP released per min/pmol of eIF2B at 30 degrees C is approximately 1 order of magnitude lower than values previously reported for the mammalian factor. Guanosine Diphosphate 163-166 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 192-197 11726195-1 2001 There are conflicting reports over the question of whether the displacement by GTP of GDP bound to eIF2 catalyzed by eIF2B follows a substituted enzyme mechanism, as is believed to be the case for other guanine nucleotide exchange factors, or is a sequential mechanism. Guanosine Diphosphate 86-89 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 117-122 11726195-5 2001 276, 24697-24703, 2001) showing displacement by eIF2B of GDP bound to eIF2 in the absence of displacing nucleotide appears to offer a way of resolving the dispute and suggests that both mechanisms may be operative. Guanosine Diphosphate 57-60 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 48-53 9345299-3 1997 Suitable rate constants for a sequential mechanism involving the transient formation of the quaternary complex eIF-2.eIF-2B.GDP.GTP are suggested. Guanosine Diphosphate 124-127 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 117-123 10216940-4 1999 The exchange of GDP bound to eIF2 for GTP is a prerequisite to binding Met-tRNAi and is mediated by a second initiation factor, eIF2B. Guanosine Diphosphate 16-19 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 128-133 8929216-2 1996 The guanine nucleotide-exchange factor eIF2B mediates the exchange of GDP bound to translation initiation factor eIF2 for GTP. Guanosine Diphosphate 70-73 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 39-44 9438375-1 1997 Eukaryotic initiation factor 2B (eIF2B) is a guanine nucleotide-exchange factor which mediates the exchange of GDP (bound to initiation factor eIF2) for GTP, thus regenerating the active [eIF2.GTP] complex that is required for peptide-chain initiation. Guanosine Diphosphate 111-114 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 0-31 9438375-1 1997 Eukaryotic initiation factor 2B (eIF2B) is a guanine nucleotide-exchange factor which mediates the exchange of GDP (bound to initiation factor eIF2) for GTP, thus regenerating the active [eIF2.GTP] complex that is required for peptide-chain initiation. Guanosine Diphosphate 111-114 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 33-38 1772451-8 1991 emphasises the interrelationships of the rate constants for the binding of GDP and of EF-Ts (eIF-2B) to the ternary complex EF-Ts.EF-Tu.GDP (eIF-2B.eIF-2.GDP). Guanosine Diphosphate 136-139 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 93-99 7982969-2 1994 Protein synthesis in mammalian cells is regulated at the level of the guanine nucleotide exchange factor, eIF-2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Guanosine Diphosphate 183-186 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 106-112 8099443-5 1993 Phosphorylation of eIF-2 alpha inhibits initiation in mammalian cells by sequestering eIF-2B, the factor required for exchange of GTP for GDP on eIF-2. Guanosine Diphosphate 138-141 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 86-92 1618793-3 1992 The released eIF-2.GDP can participate in an eIF-2B-catalyzed GDP/GTP exchange reaction to reform the Met-tRNA(f).eIF-2.GTP ternary complex. Guanosine Diphosphate 19-22 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 45-51 1618793-3 1992 The released eIF-2.GDP can participate in an eIF-2B-catalyzed GDP/GTP exchange reaction to reform the Met-tRNA(f).eIF-2.GTP ternary complex. Guanosine Diphosphate 62-65 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 45-51 1618793-5 1992 When such an 80 S initiation complex, containing bound eIF-2.GDP, was incubated with GTP and eIF-2B, GDP was released. Guanosine Diphosphate 61-64 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 93-99 1618793-5 1992 When such an 80 S initiation complex, containing bound eIF-2.GDP, was incubated with GTP and eIF-2B, GDP was released. Guanosine Diphosphate 101-104 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 93-99 1618793-11 1992 Rather, the affinity of 60 S ribosomal subunits for either eIF-2, or the eIF-2 moiety of the eIF-2.eIF-2B complex, prevents association of 60 S ribosomal subunits with eIF-2.GDP formed in the initiation reaction. Guanosine Diphosphate 174-177 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 99-105 1772451-3 1991 22, 523-533:1990) for the reactions catalysed by eIF-2B (GEF) in which free GDP exchanges with GDP bound to eIF-2 have been re-evaluated using the computational procedures developed by Chau et al. Guanosine Diphosphate 76-79 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 49-55 1772451-3 1991 22, 523-533:1990) for the reactions catalysed by eIF-2B (GEF) in which free GDP exchanges with GDP bound to eIF-2 have been re-evaluated using the computational procedures developed by Chau et al. Guanosine Diphosphate 95-98 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 49-55 1772451-8 1991 emphasises the interrelationships of the rate constants for the binding of GDP and of EF-Ts (eIF-2B) to the ternary complex EF-Ts.EF-Tu.GDP (eIF-2B.eIF-2.GDP). Guanosine Diphosphate 75-78 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 141-147 1772451-8 1991 emphasises the interrelationships of the rate constants for the binding of GDP and of EF-Ts (eIF-2B) to the ternary complex EF-Ts.EF-Tu.GDP (eIF-2B.eIF-2.GDP). Guanosine Diphosphate 136-139 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 141-147 1772451-8 1991 emphasises the interrelationships of the rate constants for the binding of GDP and of EF-Ts (eIF-2B) to the ternary complex EF-Ts.EF-Tu.GDP (eIF-2B.eIF-2.GDP). Guanosine Diphosphate 136-139 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 93-99 1772451-8 1991 emphasises the interrelationships of the rate constants for the binding of GDP and of EF-Ts (eIF-2B) to the ternary complex EF-Ts.EF-Tu.GDP (eIF-2B.eIF-2.GDP). Guanosine Diphosphate 136-139 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 141-147 2317212-1 1990 Published data have been analysed to determine the rate constants governing the exchange of GDP in the complex of the eukaryotic protein synthesis initiation factor eIF-2 with GDP, catalysed by eIF-2B. Guanosine Diphosphate 92-95 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 194-200 2076109-1 1990 The ability of eIF-2.GDP in which the alpha subunit of eIF-2 is phosphorylated (eIF-2(alpha P).GDP) to act as a competitive inhibitor of eIF-2B-catalysed exchange of eIF-2-bound GDP has been investigated by modelling data provided by Rowlands et al. Guanosine Diphosphate 21-24 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 137-143 2076109-1 1990 The ability of eIF-2.GDP in which the alpha subunit of eIF-2 is phosphorylated (eIF-2(alpha P).GDP) to act as a competitive inhibitor of eIF-2B-catalysed exchange of eIF-2-bound GDP has been investigated by modelling data provided by Rowlands et al. Guanosine Diphosphate 95-98 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 137-143 2076109-1 1990 The ability of eIF-2.GDP in which the alpha subunit of eIF-2 is phosphorylated (eIF-2(alpha P).GDP) to act as a competitive inhibitor of eIF-2B-catalysed exchange of eIF-2-bound GDP has been investigated by modelling data provided by Rowlands et al. Guanosine Diphosphate 95-98 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 137-143 2076109-7 1990 Classic double reciprocal plots for competitive inhibition were found only when [eIF-2B] was low in relation to [eIF-2 (alpha P).GDP]. Guanosine Diphosphate 129-132 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 81-87 2076109-8 1990 Relatively high cellular [eIF-2B] lessens the inhibitory effect of eIF-2(alpha P).GDP and suggests the possibility of other potential controls of initiation. Guanosine Diphosphate 82-85 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 26-32 2317212-1 1990 Published data have been analysed to determine the rate constants governing the exchange of GDP in the complex of the eukaryotic protein synthesis initiation factor eIF-2 with GDP, catalysed by eIF-2B. Guanosine Diphosphate 176-179 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 194-200 2317212-2 1990 The interaction of eIF-2B with eIF-2.GDP appears to include a very high "on" rate constant of up to 4 x 10(8) M-1 sec-1 - a value very similar to that found by others for the interaction of the bacterial elongation factors Tu and Ts. Guanosine Diphosphate 37-40 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 19-25 2317212-3 1990 Assuming a substituted enzyme mechanism that leads to displacement of GDP and ultimately to formation of a quaternary complex eIF-2B.eIF-2.GTP.methionyl-tRNA, minimum rate constants have been estimated for the additional reactions assuming in vivo rates of protein synthesis. Guanosine Diphosphate 70-73 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 126-132 3647910-2 1987 Two mechanisms have been considered--first a displacement reaction in which eIF-2B displaces GDP and GTP in a manner analogous to a "ping-pong" enzyme mechanism, and secondly the possibility that binding of eIF-2B to eIF-2 nucleotide complexes enhances the rate of nucleotide exchange without itself inducing nucleotide displacement. Guanosine Diphosphate 93-96 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 76-82 2679904-3 1989 In a later step, eIF-2 is released from the ribosomal initiation complex, most likely as an eIF-2.GDP complex, and another initiation factor termed eIF-2B is necessary to recycle eIF-2 by displacing GDP by GTP. Guanosine Diphosphate 199-202 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 148-154 3647910-2 1987 Two mechanisms have been considered--first a displacement reaction in which eIF-2B displaces GDP and GTP in a manner analogous to a "ping-pong" enzyme mechanism, and secondly the possibility that binding of eIF-2B to eIF-2 nucleotide complexes enhances the rate of nucleotide exchange without itself inducing nucleotide displacement. Guanosine Diphosphate 93-96 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 207-213 3647910-8 1987 Minimum rate constants for the formation of eIF-2 X eIF-2B from eIF-2 X GDP and eIF-2 X GTP and reverse reactions are derived. Guanosine Diphosphate 72-75 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 52-58 29425030-6 2018 The available evidence leads to the conclusion that eIF2 is channeled from the ribosome (as an eIF5 eIF2-GDP complex) to eIF2B, converted by eIF2B to the TC, which is then channeled back to eIF5 and the ribosome. Guanosine Diphosphate 105-108 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 121-126 3844338-9 1985 Consideration of the kinetic parameters favours the formation of a ternary complex of eIF-2 X eIF-2B X GDP en route to eIF-2 X GTP as opposed to displacement of GDP from eIF-2 X GDP by eIF-2B. Guanosine Diphosphate 103-106 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 94-100 3844338-9 1985 Consideration of the kinetic parameters favours the formation of a ternary complex of eIF-2 X eIF-2B X GDP en route to eIF-2 X GTP as opposed to displacement of GDP from eIF-2 X GDP by eIF-2B. Guanosine Diphosphate 103-106 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 185-191 29716790-1 2018 A key site of translation control is the phosphorylation of the eukaryotic translation initiation factor 2alpha (eIF2alpha), which reduces the rate of GDP to GTP exchange by eIF2B, leading to altered translation. Guanosine Diphosphate 151-154 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 174-179 3844338-5 1985 Addition of eIF-2B has the effect of raising k-1 for both GDP and GTP several hundred-fold and k+1 50- and 7000-fold, respectively. Guanosine Diphosphate 58-61 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 12-18 29425030-6 2018 The available evidence leads to the conclusion that eIF2 is channeled from the ribosome (as an eIF5 eIF2-GDP complex) to eIF2B, converted by eIF2B to the TC, which is then channeled back to eIF5 and the ribosome. Guanosine Diphosphate 105-108 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 141-146 26705305-4 2016 The suppression of eIF2alpha phosphorylation via MPT synthase and ATAC prevented sequestration of the guanine nucleotide exchange factor eIF2B, which recycles eIF2-GDP to eIF2-GTP, resulting in the promotion of translation initiation. Guanosine Diphosphate 164-167 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 137-142 29036434-3 2017 Following GTP hydrolysis, eIF2-GDP is recycled back to TC by its guanine nucleotide exchange factor (GEF), eIF2B. Guanosine Diphosphate 31-34 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 107-112 29036434-8 2017 We present: (i) a novel mechanism for the inhibition of eIF2B activity, whereby eIF2alpha phosphorylation destabilizes an autoregulatory intramolecular interaction within eIF2alpha; and (ii) the first structural model for the complex of eIF2B with its substrate, eIF2-GDP, reaction intermediates, apo-eIF2 and eIF2-GTP, and product, TC, with direct implications for the eIF2B catalytic mechanism. Guanosine Diphosphate 268-271 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 56-61 25486352-7 2014 Our results demonstrate that the eIF2Bgamma subunit is required for eIF2B to gain access to eIF2 GDP. Guanosine Diphosphate 97-100 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 33-38 21204011-1 2010 Eukaryotic translation initiation factor eIF2B, the guanine nucleotide exchange factor (GEF) for eIF2, catalyzes conversion of eIF2 GDP to eIF2 GTP. Guanosine Diphosphate 132-135 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 41-46 16157703-2 2005 eIF2B is the heteropentameric guanine nucleotide exchange factor that converts eIF2, from an inactive guanosine diphosphate-bound complex to eIF2-guanosine triphosphate. Guanosine Diphosphate 102-123 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 0-5 18631136-7 2008 eIF2B then re-activates eIF2 by removing GDP, thereby promoting association of GTP. Guanosine Diphosphate 41-44 eukaryotic translation initiation factor 2B subunit delta Homo sapiens 0-5