PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18682040-4 2008 When ROS containing bleached rhodopsin (R(*)) were centrifuged in low ionic strength buffer, G(t-) remained associated with the membrane fraction, whereas G(t-)GDP remained in the soluble fraction. Guanosine Diphosphate 160-163 rhodopsin Homo sapiens 29-38 18487375-5 2008 The ability of mutant rhodopsin to activate transducin constitutively was monitored by measuring the catalytic exchange of bound GDP for radiolabeled [(35)S]GTPgammaS in transducin. Guanosine Diphosphate 129-132 rhodopsin Homo sapiens 22-31 18682040-6 2008 The results also suggest that G(t-), rather than G(t-)GDP, is the moiety which exhibits tight, "light-induced" binding to rhodopsin. Guanosine Diphosphate 54-57 rhodopsin Homo sapiens 122-131 17011013-1 2006 In the early steps of visual signal transduction, light-activated rhodopsin (R*) catalyzes GDP/GTP exchange in the heterotrimeric G protein (Galphabetagamma) transducin. Guanosine Diphosphate 91-94 rhodopsin Homo sapiens 66-75 17059215-7 2006 Here, it is shown that both light-activated rhodopsin and the soluble mimic of R form trapped intermediate complexes with a GDP-released "empty pocket" state of the heterotrimer in the absence of GTP (or GTPgammaS). Guanosine Diphosphate 124-127 rhodopsin Homo sapiens 44-53 12146960-11 2002 Interaction of the resulting spin-labeled Galphabetagamma with photoactivated rhodopsin, followed by rhodopsin-catalyzed GTPgammaS binding, caused the amino-terminal domain of Galpha to revert to a dynamically disordered state similar to that of the GDP-bound form. Guanosine Diphosphate 250-253 rhodopsin Homo sapiens 78-87 12146960-11 2002 Interaction of the resulting spin-labeled Galphabetagamma with photoactivated rhodopsin, followed by rhodopsin-catalyzed GTPgammaS binding, caused the amino-terminal domain of Galpha to revert to a dynamically disordered state similar to that of the GDP-bound form. Guanosine Diphosphate 250-253 rhodopsin Homo sapiens 101-110 12596920-2 2002 After GTP/GDP exchange on the a subunit of transducin (Talpha) by illuminated rhodopsin, the GTP-bound form Talpha (GTP/Talpha) interacts with the regulatory subunit (Pgamma) of PDE6 to activate cGMP hydrolytic activity. Guanosine Diphosphate 10-13 rhodopsin Homo sapiens 78-87 12206508-5 2002 Interestingly, PLP modified both the alpha- and beta-subunits of T. Moreover, PLP in the presence of GDP behaved as a GTP analog, since this mixture was capable of dissociating T from T:photoactivated rhodopsin complexes. Guanosine Diphosphate 101-104 rhodopsin Homo sapiens 201-210 9054576-5 1997 Here, an extensive survey is done of farnesylcysteine analogs and other lipid molecules, which are tested for their ability to inhibit GTP/GDP exchange in transducin catalyzed by photolyzed rhodopsin. Guanosine Diphosphate 139-142 rhodopsin Homo sapiens 190-199 9539726-5 1998 Docking of the NMR structure to the GDP-bound x-ray structure of Gt reveals that photoexcited rhodopsin promotes the formation of a continuous helix over residues 325-346 terminated by the C-terminal helical cap with a unique cluster of crucial hydrophobic side chains. Guanosine Diphosphate 36-39 rhodopsin Homo sapiens 94-103 1905716-3 1991 Rhodopsin facilitates the exchange of GTP gamma S for GDP bound to Gt alpha beta gamma with a 60-fold higher apparent affinity than for Gt alpha alone. Guanosine Diphosphate 54-57 rhodopsin Homo sapiens 0-9 1512243-5 1992 The rhodopsin-promoted GDP/guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) exchange reaction, within the rhodopsin-alpha T complex, then results in the dissociation of the alpha TGTP gamma S species from the rhodopsin-containing phospholipid vesicles. Guanosine Diphosphate 23-26 rhodopsin Homo sapiens 4-13 1512243-5 1992 The rhodopsin-promoted GDP/guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) exchange reaction, within the rhodopsin-alpha T complex, then results in the dissociation of the alpha TGTP gamma S species from the rhodopsin-containing phospholipid vesicles. Guanosine Diphosphate 23-26 rhodopsin Homo sapiens 107-116 1512243-5 1992 The rhodopsin-promoted GDP/guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) exchange reaction, within the rhodopsin-alpha T complex, then results in the dissociation of the alpha TGTP gamma S species from the rhodopsin-containing phospholipid vesicles. Guanosine Diphosphate 23-26 rhodopsin Homo sapiens 107-116 1317509-2 1992 Light-activated rhodopsin catalyses the exchange of GDP for GTP on multiple transducin molecules. Guanosine Diphosphate 52-55 rhodopsin Homo sapiens 16-25 8810308-0 1996 Modulation of GDP release from transducin by the conserved Glu134-Arg135 sequence in rhodopsin. Guanosine Diphosphate 14-17 rhodopsin Homo sapiens 85-94 8810308-3 1996 The cytoplasmic domain of rhodopsin binds and activates Gt, but residues that stimulate GDP release from Gt have not been identified until now. Guanosine Diphosphate 88-91 rhodopsin Homo sapiens 26-35 8810308-5 1996 We propose that Glu134 and Arg135 constitute the site that directly provides the signal from rhodopsin to activate GDP release from Gt. Guanosine Diphosphate 115-118 rhodopsin Homo sapiens 93-102 2040617-7 1991 Conditions which resulted in the activation of the alpha T.GDP subunit (i.e. the addition of AlF4- or the addition of rhodopsin-containing vesicles and GTP gamma S) resulted in a reversal of the alpha T.GDP-induced enhancement of the MIANS beta gamma T fluorescence. Guanosine Diphosphate 59-62 rhodopsin Homo sapiens 118-127 2040617-7 1991 Conditions which resulted in the activation of the alpha T.GDP subunit (i.e. the addition of AlF4- or the addition of rhodopsin-containing vesicles and GTP gamma S) resulted in a reversal of the alpha T.GDP-induced enhancement of the MIANS beta gamma T fluorescence. Guanosine Diphosphate 203-206 rhodopsin Homo sapiens 118-127 2604728-1 1989 The absorption of light by rhodopsin leads to the formation of an activated intermediate (R*) capable of catalysing the exchange of GTP for GDP in a retinal guanine-nucleotide-binding regulatory protein (transducin). Guanosine Diphosphate 140-143 rhodopsin Homo sapiens 27-36 2229054-1 1990 We have studied the effect of GDP and its analog guanyl-5"-yl thiophosphate (GDP beta S) on the interaction between rhodopsin and transducin (Gt). Guanosine Diphosphate 30-33 rhodopsin Homo sapiens 116-125 2229054-3 1990 Extra-MII can be completely abolished by GDP, with a half-suppression at 10 microM under the conditions (4 degrees C, pH 8, 7.5 nM photoactivated rhodopsin). Guanosine Diphosphate 41-44 rhodopsin Homo sapiens 146-155 2229054-9 1990 We discuss a generalized induced fit mechanism, where MII induces opening of the Gt nucleotide site and release of GDP which in turn is obligatory to establish the MII-stabilizing rhodopsin-Gt three-loop interaction (Konig, B., Arendt, A., McDowell, J.H., Kahlert, M., Hargrave, P.A., and Hofmann, K.P. Guanosine Diphosphate 115-118 rhodopsin Homo sapiens 180-189 2229054-15 1990 The GDP beta S/GDP difference is discussed in terms of bound GDP disturbing the interaction with two and GDP beta S with only one of the rhodopsin binding sites. Guanosine Diphosphate 4-7 rhodopsin Homo sapiens 137-146 2229054-15 1990 The GDP beta S/GDP difference is discussed in terms of bound GDP disturbing the interaction with two and GDP beta S with only one of the rhodopsin binding sites. Guanosine Diphosphate 15-18 rhodopsin Homo sapiens 137-146 1697545-3 1990 Light-induced conformational changes in rhodopsin facilitate the binding of a guanosine nucleotide-binding protein, transducin, which then undergoes a GTP-GDP exchange reaction and dissociation of the transducin complex. Guanosine Diphosphate 155-158 rhodopsin Homo sapiens 40-49 2119661-0 1990 Surfaces of interaction between Gt and rhodopsin in the GDP-bound and empty-pocket configurations. Guanosine Diphosphate 56-59 rhodopsin Homo sapiens 39-48 2169289-3 1990 When light bleaches rhodopsin there is an induced exchange of GTP for GDP bound to the alpha subunit of the retinal G-protein, transducin (T). Guanosine Diphosphate 70-73 rhodopsin Homo sapiens 20-29 2826123-5 1987 Activation requires that GDP or a suitable analogue be bound to T alpha: T alpha-GDP and T alpha-GDP alpha S are activable by fluorides, but not T alpha-GDP beta S, nor T alpha that has released its nucleotide upon binding to photoexcited rhodopsin. Guanosine Diphosphate 25-28 rhodopsin Homo sapiens 239-248 2509200-2 1989 In the first step of the visual transduction cascade a photoexcited rhodopsin molecule, R*ret, binds to a GDP-carrying transducin molecule, TGDP. Guanosine Diphosphate 106-109 rhodopsin Homo sapiens 68-77 3139484-3 1988 Receptors such as those for beta- and alpha-adrenergic catecholamines, muscarinic agonists, and the retinal photoreceptor rhodopsin, catalyze the exchange of GDP for GTP binding to the alpha subunit of a specific G protein. Guanosine Diphosphate 158-161 rhodopsin Homo sapiens 122-131 3102494-2 1987 Photolyzed rhodopsin acts in a catalytic manner to mediate the exchange of GTP for GDP bound to transducin. Guanosine Diphosphate 83-86 rhodopsin Homo sapiens 11-20 3924910-7 1985 GTP/GDP exchange at the G-protein after interaction with rhodopsin does not reduce the accessibility of the relevant SH group. Guanosine Diphosphate 4-7 rhodopsin Homo sapiens 57-66 3001052-2 1986 Photo-excited rhodopsin activates a guanine nucleotide-binding protein (G-protein) by catalyzing the exchange of bound GDP for GTP. Guanosine Diphosphate 119-122 rhodopsin Homo sapiens 14-23 2423011-4 1986 Photoexcited rhodopsin triggers transducin by catalyzing the exchange of GTP for bound GDP. Guanosine Diphosphate 87-90 rhodopsin Homo sapiens 13-22 28655769-1 2017 The visual photo-transduction cascade is a prototypical G protein-coupled receptor (GPCR) signaling system, in which light-activated rhodopsin (Rho*) is the GPCR catalyzing the exchange of GDP for GTP on the heterotrimeric G protein transducin (GT). Guanosine Diphosphate 189-192 rhodopsin Homo sapiens 133-142 6310406-3 1983 As a result of interaction with a rhodopsin photoproduct (possibly metarhodopsin II380), this GTP-binding protein exchanges a previously bound GDP for a GTP. Guanosine Diphosphate 143-146 rhodopsin Homo sapiens 34-43 6301538-2 1983 The phosphorylation of rhodopsin, the major protein-staining band (Mr approximately 34 000-38 000), was markedly and specifically increased by exposure of rod outer segments to light; various guanine nucleotides (10 microM) including GMP, GDP, and GTP also specifically increased rhodopsin phosphorylation (up to 5-fold). Guanosine Diphosphate 239-242 rhodopsin Homo sapiens 23-32 6601965-3 1983 These sites possess high affinity to GDP (Kd less than 10(-6) M) in dark-adapted preparations, and in the presence of bleached rhodopsin they effectively bind the non-hydrolizable analog of GTP--GPP (NH) P (Kd less than 10(-6) M). Guanosine Diphosphate 37-40 rhodopsin Homo sapiens 127-136 3938969-10 1985 In the presence of G beta gamma and photolyzed rhodopsin, GDP and GDP beta S, but not Gpp(NH)p and GTP gamma S, increased the ADP-ribosylation of Gi alpha. Guanosine Diphosphate 58-61 rhodopsin Homo sapiens 47-56 6090950-2 1984 Photoexcited rhodopsin (R*) binds to a multisubunit membrane protein called transducin (T) and stimulates the exchange of a bound GDP molecule for GTP. Guanosine Diphosphate 130-133 rhodopsin Homo sapiens 13-22 6930647-0 1980 Photolyzed rhodopsin catalyzes the exchange of GTP for bound GDP in retinal rod outer segments. Guanosine Diphosphate 61-64 rhodopsin Homo sapiens 11-20 6930647-9 1980 This corresponds to the catalyzed exchange of 500 p[NH]ppG for bound GDP per photolyzed rhodopsin. Guanosine Diphosphate 69-72 rhodopsin Homo sapiens 88-97 209180-2 1978 The hydrolysis of guanosine triphosphate (GTP) and the consequent formation of guanosine diphosphate (GDP) and phosphate (P1) are activated by light in a suspension of broken retinal rods: the hydrolysis rate with GTP in the micrometer concentration range is 2.5-3.5 n-mole/min per mg of rhodopsin in the preparation. Guanosine Diphosphate 79-100 rhodopsin Homo sapiens 288-297 209180-2 1978 The hydrolysis of guanosine triphosphate (GTP) and the consequent formation of guanosine diphosphate (GDP) and phosphate (P1) are activated by light in a suspension of broken retinal rods: the hydrolysis rate with GTP in the micrometer concentration range is 2.5-3.5 n-mole/min per mg of rhodopsin in the preparation. Guanosine Diphosphate 102-105 rhodopsin Homo sapiens 288-297 33453187-2 2021 Light absorption by rhodopsin leads to the activation of transducin as a result of the exchange of its GDP for GTP. Guanosine Diphosphate 103-106 rhodopsin Homo sapiens 20-29 23458690-1 2013 Upon illumination the visual receptor rhodopsin (Rho) transitions to the activated form Rho(*), which binds the heterotrimeric G protein, transducin (Gt) causing GDP to GTP exchange and Gt dissociation. Guanosine Diphosphate 162-165 rhodopsin Homo sapiens 38-47