PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 11529678-9 2001 Similarly, introduction of this contact in wild-type Galpha(i1) decreased the GDP release rate of Galpha(i1) by half. Guanosine Diphosphate 78-81 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 53-62 11976690-4 2002 Here we describe the structural determinants of GoLoco activity as revealed by the crystal structure of Galpha(i1) GDP bound to the GoLoco region of the "regulator of G-protein signalling" protein RGS14. Guanosine Diphosphate 115-118 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 104-113 11529678-9 2001 Similarly, introduction of this contact in wild-type Galpha(i1) decreased the GDP release rate of Galpha(i1) by half. Guanosine Diphosphate 78-81 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 98-107 22952234-4 2012 The crystal structures of Galpha(i) GDP in complex with LGN GL3 and GL4, respectively, reveal distinct GL/Galpha(i) interaction features when compared with the only high resolution structure known with GL/Galpha(i) interaction between RGS14 and Galpha(i1.) Only a few residues C-terminal to the conserved GL sequence are required for LGN GLs to bind to Galpha(i) GDP. Guanosine Diphosphate 36-39 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 245-255 21880739-4 2011 The non-receptor guanine nucleotide exchange factor Ric-8A can bind and act on the RGS14 Galpha(i1)-GDP complex to play a role in unconventional G protein signaling independent of G protein-coupled receptors (GPCRs). Guanosine Diphosphate 100-103 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 89-98 19437048-2 2009 Using these methodologies, binding of the GoLoco motif peptide to the Galpha(i1) subunit was found to restrict the relative movement of the helical and catalytic domains in the Galpha(i1) subunit, which is in agreement with a proposed mechanism of GDP dissociation inhibition by GoLoco motif proteins. Guanosine Diphosphate 248-251 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 70-79 20679342-7 2010 The binding of sNUCB1 to Galpha(i1) inhibits its basal rate of GDP release and slows its rate and extent of GTPgammaS uptake. Guanosine Diphosphate 63-66 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 25-34 19703944-6 2009 These results demonstrate that the guanine nucleotide exchange reactions of Galpha(i1) are drastically modulated by detergents differently depending on the type and the state (monomeric or micellar) of the detergents and that dissociation of GDP from Galpha(i1) does not necessarily lead to immediate binding of GTP to Galpha(i1) in some cases. Guanosine Diphosphate 242-245 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 76-85 19703944-6 2009 These results demonstrate that the guanine nucleotide exchange reactions of Galpha(i1) are drastically modulated by detergents differently depending on the type and the state (monomeric or micellar) of the detergents and that dissociation of GDP from Galpha(i1) does not necessarily lead to immediate binding of GTP to Galpha(i1) in some cases. Guanosine Diphosphate 242-245 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 251-260 19703944-6 2009 These results demonstrate that the guanine nucleotide exchange reactions of Galpha(i1) are drastically modulated by detergents differently depending on the type and the state (monomeric or micellar) of the detergents and that dissociation of GDP from Galpha(i1) does not necessarily lead to immediate binding of GTP to Galpha(i1) in some cases. Guanosine Diphosphate 242-245 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 251-260 19437048-2 2009 Using these methodologies, binding of the GoLoco motif peptide to the Galpha(i1) subunit was found to restrict the relative movement of the helical and catalytic domains in the Galpha(i1) subunit, which is in agreement with a proposed mechanism of GDP dissociation inhibition by GoLoco motif proteins. Guanosine Diphosphate 248-251 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 177-186 16949794-9 2007 In contrast, the GoLoco motif of Rap1GAP1b, which is canonical in primary sequence, was found to interact with Galpha(i1).GDP. Guanosine Diphosphate 122-125 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 111-120 18541531-6 2008 Pulldown assays, gel filtration, isothermal titration calorimetry, and rapid mixing stopped-flow fluorescence spectroscopy indicate that Ric-8A catalyzes the rapid release of GDP from AGS3-C:Galpha(i1).GDP. Guanosine Diphosphate 175-178 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 191-200 19703466-3 2009 For example, mutant Galpha(i1)-T329A shows an 18-fold increase in basal GDP release rate and, when expressed in culture, it causes a significant decrease in forskolin-stimulated cAMP accumulation. Guanosine Diphosphate 72-75 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 20-29 19703466-4 2009 The crystal structure of Galpha(i1)-T329A.GDP shows substantial conformational rearrangement of the switch I region and additional striking alterations of side chains lining the catalytic pocket that disrupt the Mg(+2) coordination sphere and dislodge bound Mg(+2). Guanosine Diphosphate 42-45 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 25-34 19369247-7 2009 Injection of this peptide into cultured cells expressing eYFP-Galpha(i1)(GDP) and eCFP-Gbetagamma reduces the overall association of the subunits suggesting this site is operative in cells. Guanosine Diphosphate 73-76 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 62-71 18541531-8 2008 Subsequent dissociation of AGS3-C and GDP from Galpha(i1) yields a stable nucleotide free Ric-8A.Galpha(i1) complex that, in the presence of GTP, dissociates to yield Ric-8A and Galpha(i1).GTP. Guanosine Diphosphate 38-41 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 47-56 18541531-8 2008 Subsequent dissociation of AGS3-C and GDP from Galpha(i1) yields a stable nucleotide free Ric-8A.Galpha(i1) complex that, in the presence of GTP, dissociates to yield Ric-8A and Galpha(i1).GTP. Guanosine Diphosphate 38-41 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 97-106 18541531-8 2008 Subsequent dissociation of AGS3-C and GDP from Galpha(i1) yields a stable nucleotide free Ric-8A.Galpha(i1) complex that, in the presence of GTP, dissociates to yield Ric-8A and Galpha(i1).GTP. Guanosine Diphosphate 38-41 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 97-106 18541531-10 2008 The action of Ric-8A on AGS3:Galpha(i1).GDP ensures unidirectional activation of Galpha subunits that cannot be reversed by AGS3. Guanosine Diphosphate 40-43 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 29-38 16338227-2 2006 Recently, several peptides that bind heterotrimeric G-protein alpha subunits have been isolated including the novel Galpha(i1).GDP binding peptides R6A and KB-752. Guanosine Diphosphate 127-130 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 116-125 16981699-7 2006 The crystal structure of KB-1753 bound to G alpha(i1) x GDP x AlF4(-) reveals binding to a conserved hydrophobic groove between switch II and alpha3 helices and, along with supporting biochemical data and previous structural analyses, supports the notion that this is the site of effector interactions for G alpha(i) subunits. Guanosine Diphosphate 56-59 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 44-52 16338227-3 2006 The R6A peptide and its minimized derivative R6A-1 interact with Galpha(i1).GDP. Guanosine Diphosphate 76-79 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 65-74 15917656-7 2005 However, preincubation of RGS14 with Galpha(i1)-GDP precludes either from promoting microtubule polymerization, suggesting that a functional GTP/GDP cycle is necessary. Guanosine Diphosphate 48-51 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 37-46 16051611-2 2005 The peptide was minimized to a 9-residue sequence (R6A-1) that retains high affinity and specificity for the GDP-bound state of Galpha(i1) and acts as a guanine nucleotide dissociation inhibitor (GDI). Guanosine Diphosphate 109-112 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 128-137 15917656-7 2005 However, preincubation of RGS14 with Galpha(i1)-GDP precludes either from promoting microtubule polymerization, suggesting that a functional GTP/GDP cycle is necessary. Guanosine Diphosphate 145-148 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 37-46 14656218-7 2004 In binding assays with the non-hydrolysable GTP analogue guanosine 5"-[gamma-thio]triphosphate, G18 exhibits GDI activity, slowing the exchange of GDP for GTP by Galpha(i1). Guanosine Diphosphate 147-150 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 162-171 16004878-7 2005 Structural determination of the Galpha(i1)/peptide complex reveals unique changes in the Galpha switch regions predicted to enhance nucleotide exchange by creating a GDP dissociation route. Guanosine Diphosphate 166-169 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 32-41 14656218-8 2004 Only the first and third GoLoco motifs within G18 are capable of interacting with Galpha subunits, and these bind with low micromolar affinity only to Galpha(i1) in the GDP-bound form, and not to Galpha(o), Galpha(q), Galpha(s) or Galpha12. Guanosine Diphosphate 169-172 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 151-160