PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 29184002-3 2018 Using SNARE-decorated proteoliposomes that cannot fuse on their own, we now demonstrate that full fusion activity can be achieved by just four soluble factors: a soluble SNARE (Vam7), a guanine nucleotide exchange factor (GEF, Mon1-Ccz1), a Rab-GDP dissociation inhibitor (GDI) complex (prenylated Ypt7-GDI), and a Rab effector complex (HOPS). Guanosine Diphosphate 245-248 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 222-225 2491852-12 1989 The GEF-dependent release of eIF-2-bound GDP was studied at several constant concentrations of one substrate (GTP or eIF-2.GDP) while varying the second substrate concentration, and the results were then plotted according to the Lineweaver-Burk method. Guanosine Diphosphate 123-126 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 4-7 2491852-13 1989 Taken together, the results of GTP and eIF-2.GDP binding to GEF and the pattern of the double-reciprocal plots strongly suggest that the guanine nucleotide exchange reaction follows a sequential mechanism. Guanosine Diphosphate 45-48 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 60-63 29432733-4 2018 We found that the catalytic domain of DOCK1, a Rac-specific guanine nucleotide exchange factor (GEF) implicated in malignancy of a variety of cancers, can greatly accelerate the GDP/GTP exchange of Rac1P29S. Guanosine Diphosphate 178-181 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 60-94 29432733-4 2018 We found that the catalytic domain of DOCK1, a Rac-specific guanine nucleotide exchange factor (GEF) implicated in malignancy of a variety of cancers, can greatly accelerate the GDP/GTP exchange of Rac1P29S. Guanosine Diphosphate 178-181 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 96-99 2491852-9 1989 The release of bound GDP is dependent on the presence of both GTP and GEF, and this argues against the possibility of a substituted enzyme (ping-pong) mechanism for the guanine nucleotide exchange reaction. Guanosine Diphosphate 21-24 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 70-73 2491852-12 1989 The GEF-dependent release of eIF-2-bound GDP was studied at several constant concentrations of one substrate (GTP or eIF-2.GDP) while varying the second substrate concentration, and the results were then plotted according to the Lineweaver-Burk method. Guanosine Diphosphate 41-44 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 4-7 29036434-3 2017 Following GTP hydrolysis, eIF2-GDP is recycled back to TC by its guanine nucleotide exchange factor (GEF), eIF2B. Guanosine Diphosphate 31-34 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 65-99 29530990-1 2018 OBJECTIVE: RASGRF1 is a guanine nucleotide exchange factor, which promotes the release of GDP from inactive Ras and stabilizes the apoprotein. Guanosine Diphosphate 90-93 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 11-18 29036434-3 2017 Following GTP hydrolysis, eIF2-GDP is recycled back to TC by its guanine nucleotide exchange factor (GEF), eIF2B. Guanosine Diphosphate 31-34 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 101-104 24597981-1 2014 Rasgrf-1 is a guanine exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Guanosine Diphosphate 75-78 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 0-8 26438819-1 2015 Phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger 2 (PREX2) is a guanine nucleotide exchange factor (GEF) for the Ras-related C3 botulinum toxin substrate 1 (Rac1) GTPase, facilitating the exchange of GDP for GTP on Rac1. Guanosine Diphosphate 223-226 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 87-121 26438819-1 2015 Phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger 2 (PREX2) is a guanine nucleotide exchange factor (GEF) for the Ras-related C3 botulinum toxin substrate 1 (Rac1) GTPase, facilitating the exchange of GDP for GTP on Rac1. Guanosine Diphosphate 223-226 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 123-126 25991726-9 2015 Thus, SBDS acts as a guanine nucleotide exchange factor (GEF) for EFL1 promoting its activation by the release of GDP. Guanosine Diphosphate 114-117 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 21-55 25991726-9 2015 Thus, SBDS acts as a guanine nucleotide exchange factor (GEF) for EFL1 promoting its activation by the release of GDP. Guanosine Diphosphate 114-117 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 57-60 21111786-6 2011 The RasGrf proteins exhibit modular structures composed by multiple domains including CDC25H and DHPH motifs responsible for promoting GDP/GTP exchange, respectively, on Ras or Rho GTPase targets. Guanosine Diphosphate 135-138 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 4-10 23422181-1 2013 BACKGROUND: T-lymphoma and metastasis gene 1 (Tiam1) produces a guanine nucleotide exchange factor (GNEF) that regulates guanosine triphosphatase, which transforms guanosine diphosphate to guanosine triphosphate. Guanosine Diphosphate 164-185 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 64-98 23422181-1 2013 BACKGROUND: T-lymphoma and metastasis gene 1 (Tiam1) produces a guanine nucleotide exchange factor (GNEF) that regulates guanosine triphosphatase, which transforms guanosine diphosphate to guanosine triphosphate. Guanosine Diphosphate 164-185 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 100-104 23048039-3 2012 CONCLUSION: GDP/GTP exchange in the GEF domain controls the MIU domain interactions with the ubiquitinated cargos. Guanosine Diphosphate 12-15 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 36-39 22075848-1 2011 T lymphoma and metastasis gene 1 (Tiam1) is a guanine nucleotide exchange factor (GNEF) that regulates the guanosine triphosphatase to facilitate the exchange of guanosine diphosphate for guanosine triphosphate. Guanosine Diphosphate 162-183 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 46-80 22075848-1 2011 T lymphoma and metastasis gene 1 (Tiam1) is a guanine nucleotide exchange factor (GNEF) that regulates the guanosine triphosphatase to facilitate the exchange of guanosine diphosphate for guanosine triphosphate. Guanosine Diphosphate 162-183 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 82-86 25332840-1 2013 The proteins that possess guanine nucleotide exchange factor (GEF) activity, which include about ~800 G protein coupled receptors (GPCRs),1 15 Arf GEFs,2 81 Rho GEFs,3 8 Ras GEFs,4 and others for other families of GTPases,5 catalyze the exchange of GTP for GDP on all regulatory guanine nucleotide binding proteins. Guanosine Diphosphate 257-260 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 62-65 22960035-1 2013 We report the novel finding that Phospholipase D2 (PLD2), through its PX and PH domains, binds specifically to Ras and catalyzes the GDP/GTP exchange (i.e., is a GEF), with potency comparable to Ras-GRF-1, a known Ras-GEF. Guanosine Diphosphate 133-136 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 195-204 23091056-5 2012 Human Ric1 and Rgp1 both bind Rab6A with preference for the GDP-bound conformation, characteristic of a GEF. Guanosine Diphosphate 60-63 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 104-107 22106281-1 2011 We have discovered that the enzyme phospholipase D2 (PLD2) binds directly to the small GTPase Rac2, resulting in PLD2 functioning as a guanine nucleotide exchange factor (GEF), because it switches Rac2 from the GDP-bound to the GTP-bound states. Guanosine Diphosphate 211-214 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 135-169 22106281-1 2011 We have discovered that the enzyme phospholipase D2 (PLD2) binds directly to the small GTPase Rac2, resulting in PLD2 functioning as a guanine nucleotide exchange factor (GEF), because it switches Rac2 from the GDP-bound to the GTP-bound states. Guanosine Diphosphate 211-214 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 171-174 19642867-7 2009 In vitro GTP/GDP binding analyses demonstrate different affinity of RhoC for GTP[S] and faster intrinsic and guanine nucleotide exchange factor (GEF)-stimulated GDP/GTP exchange rates compared to RhoA. Guanosine Diphosphate 13-16 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 109-143 19642867-7 2009 In vitro GTP/GDP binding analyses demonstrate different affinity of RhoC for GTP[S] and faster intrinsic and guanine nucleotide exchange factor (GEF)-stimulated GDP/GTP exchange rates compared to RhoA. Guanosine Diphosphate 161-164 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 145-148 21158412-4 2011 Here we report that Ric-8A, a nonreceptor guanine nucleotide exchange factor (GEF), functionally interacts with the RGS14-Galphai1-GDP signaling complex to regulate its activation state. Guanosine Diphosphate 131-134 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 42-76 21158412-4 2011 Here we report that Ric-8A, a nonreceptor guanine nucleotide exchange factor (GEF), functionally interacts with the RGS14-Galphai1-GDP signaling complex to regulate its activation state. Guanosine Diphosphate 131-134 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 78-81 21204011-1 2010 Eukaryotic translation initiation factor eIF2B, the guanine nucleotide exchange factor (GEF) for eIF2, catalyzes conversion of eIF2 GDP to eIF2 GTP. Guanosine Diphosphate 132-135 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 52-86 21204011-1 2010 Eukaryotic translation initiation factor eIF2B, the guanine nucleotide exchange factor (GEF) for eIF2, catalyzes conversion of eIF2 GDP to eIF2 GTP. Guanosine Diphosphate 132-135 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 88-91 19642867-7 2009 In vitro GTP/GDP binding analyses demonstrate different affinity of RhoC for GTP[S] and faster intrinsic and guanine nucleotide exchange factor (GEF)-stimulated GDP/GTP exchange rates compared to RhoA. Guanosine Diphosphate 13-16 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 145-148 19642867-7 2009 In vitro GTP/GDP binding analyses demonstrate different affinity of RhoC for GTP[S] and faster intrinsic and guanine nucleotide exchange factor (GEF)-stimulated GDP/GTP exchange rates compared to RhoA. Guanosine Diphosphate 161-164 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 109-143 19692568-1 2009 RasGRF family guanine nucleotide exchange factors (GEFs) promote guanosine diphosphate (GDP)/guanosine triphosphate (GTP) exchange on several Ras GTPases, including H-Ras and TC21. Guanosine Diphosphate 65-86 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 0-6 19692568-1 2009 RasGRF family guanine nucleotide exchange factors (GEFs) promote guanosine diphosphate (GDP)/guanosine triphosphate (GTP) exchange on several Ras GTPases, including H-Ras and TC21. Guanosine Diphosphate 88-91 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 0-6 15709769-4 2005 Herein we have examined in detail the catalyzed GDP/GTP exchange reaction mechanism by a Ras specific GEF, GRF1. Guanosine Diphosphate 48-51 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 107-111 15709769-8 2005 Furthermore, we found that Ras was capable of binding pyrophosphate (PPi) with a dissociation constant of 26 microM and that PPi and GMP, but neither alone, synergistically potentiated the GRF1-stimulated GDP dissociation from Ras. Guanosine Diphosphate 205-208 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 189-193 17389601-6 2007 Experimental evidence indicated that this phenomenon was associated with destabilization and ubiquitylation of Ras-GRF1, a guanine nucleotide exchange factor that activates H-Ras by facilitating the release of GDP. Guanosine Diphosphate 210-213 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 111-119 17075039-1 2006 The Ras-specific guanine nucleotide-exchange factors Son of sevenless (Sos) and Ras guanine nucleotide-releasing factor 1 (RasGRF1) transduce extracellular stimuli into Ras activation by catalyzing the exchange of Ras-bound GDP for GTP. Guanosine Diphosphate 224-227 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 80-121 17075039-1 2006 The Ras-specific guanine nucleotide-exchange factors Son of sevenless (Sos) and Ras guanine nucleotide-releasing factor 1 (RasGRF1) transduce extracellular stimuli into Ras activation by catalyzing the exchange of Ras-bound GDP for GTP. Guanosine Diphosphate 224-227 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 123-130 15557278-3 2005 We now show that: 1) Trio and another Rac GEF (Tiam1) act by inducing GDP to GTP exchange on prenylated Rac1-GDP and that our earlier assertion that activation is GTP-independent is explained by contamination of p67phox preparations with GTP and/or ATP. Guanosine Diphosphate 70-73 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 42-45 15557278-6 2005 4) The ability of ATP to support GEF-induced oxidase activation is explained by ATP serving as a gamma-phosphoryl donor for a membrane-localized nucleoside diphosphate kinase (NDPK), converting GDP to GTP. Guanosine Diphosphate 194-197 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 33-36 15557278-9 2005 7) Free GDP is derived exclusively by dissociation from prenylated Rac1-GDP, mediated by GEF. Guanosine Diphosphate 8-11 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 89-92 15557278-10 2005 NDPK and GEF appear to be functionally linked in the sense that the availability of GDP, serving as substrate for NDPK, is dependent on the level of activity of GEF. Guanosine Diphosphate 84-87 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 9-12 15557278-10 2005 NDPK and GEF appear to be functionally linked in the sense that the availability of GDP, serving as substrate for NDPK, is dependent on the level of activity of GEF. Guanosine Diphosphate 84-87 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 161-164 15557278-3 2005 We now show that: 1) Trio and another Rac GEF (Tiam1) act by inducing GDP to GTP exchange on prenylated Rac1-GDP and that our earlier assertion that activation is GTP-independent is explained by contamination of p67phox preparations with GTP and/or ATP. Guanosine Diphosphate 109-112 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 42-45 10518015-4 1999 The guanine nucleotide exchange factor (GEF) Dbl targets Rho family proteins thereby stimulating their GDP/GTP exchange, and thus is believed to be involved in receptor-mediated regulation of the proteins. Guanosine Diphosphate 103-106 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 4-38 10835426-2 2000 This novel GEF, referred to as CalDAG-GEFIII, increased the GTP/GDP ratio of Ha-Ras, R-Ras, and Rap1 in 293T cells. Guanosine Diphosphate 64-67 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 11-14 15649357-5 2005 RESULTS: We show that the binding of activated Cdc42 to the Cool-2 dimer markedly enhances its ability to associate with GDP bound Rac1, resulting in a significant activation of Rac-GEF activity. Guanosine Diphosphate 121-124 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 182-185 15649357-6 2005 While the Rac-specific GEF activity of Cool-2 is mediated through the Dbl homology (DH) domain from one monomer and the Pleckstrin homology domain from the other, activated Cdc42 interacts with the DH domain, most likely opposite the DH domain binding site for GDP bound Rac. Guanosine Diphosphate 261-264 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 23-26 10882715-6 2000 GEF activity of Ras-GRF1 toward Ha-Ras, as defined by in vitro GDP binding and release assays, was augmented after tyrosine phosphorylation by ACK1. Guanosine Diphosphate 63-66 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 0-3 10882715-6 2000 GEF activity of Ras-GRF1 toward Ha-Ras, as defined by in vitro GDP binding and release assays, was augmented after tyrosine phosphorylation by ACK1. Guanosine Diphosphate 63-66 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 16-24 10608844-5 1999 Hs-RA-GEF exhibited not only a GTP-dependent binding activity to Rap1A at its RA domain but also an activity to stimulate GDP/GTP exchange of Rap1A both in vitro and in vivo at the segment containing its REM and GEF domains. Guanosine Diphosphate 122-125 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 6-9 10608844-7 1999 On the other hand, Ce-RA-GEF associated with and stimulated GDP/GTP exchange of both Ras and Rap1A. Guanosine Diphosphate 60-63 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 25-28 10518015-4 1999 The guanine nucleotide exchange factor (GEF) Dbl targets Rho family proteins thereby stimulating their GDP/GTP exchange, and thus is believed to be involved in receptor-mediated regulation of the proteins. Guanosine Diphosphate 103-106 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 40-43 9699463-0 1998 GEF-mediated GDP/GTP exchange by monomeric GTPases: a regulatory role for Mg2+? Guanosine Diphosphate 13-16 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 0-3 9699463-2 1998 An inactive GDP-bound protein is turned on by a guanine nucleotide exchange factor (GEF) that catalyzes exchange of GTP for GDP, but unfortunately little is known about the mechanism of GEF action. Guanosine Diphosphate 12-15 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 48-82 9699463-2 1998 An inactive GDP-bound protein is turned on by a guanine nucleotide exchange factor (GEF) that catalyzes exchange of GTP for GDP, but unfortunately little is known about the mechanism of GEF action. Guanosine Diphosphate 12-15 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 84-87 9699463-2 1998 An inactive GDP-bound protein is turned on by a guanine nucleotide exchange factor (GEF) that catalyzes exchange of GTP for GDP, but unfortunately little is known about the mechanism of GEF action. Guanosine Diphosphate 12-15 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 186-189 9699463-2 1998 An inactive GDP-bound protein is turned on by a guanine nucleotide exchange factor (GEF) that catalyzes exchange of GTP for GDP, but unfortunately little is known about the mechanism of GEF action. Guanosine Diphosphate 124-127 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 48-82 9699463-2 1998 An inactive GDP-bound protein is turned on by a guanine nucleotide exchange factor (GEF) that catalyzes exchange of GTP for GDP, but unfortunately little is known about the mechanism of GEF action. Guanosine Diphosphate 124-127 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 84-87 9699463-2 1998 An inactive GDP-bound protein is turned on by a guanine nucleotide exchange factor (GEF) that catalyzes exchange of GTP for GDP, but unfortunately little is known about the mechanism of GEF action. Guanosine Diphosphate 124-127 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 186-189 9699463-5 1998 Evidence in the literature highlighting an important regulatory role for Mg2+ in the mechanism of GEF-mediated GDP/GTP exchange by monomeric GTPases is summarized. Guanosine Diphosphate 111-114 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 98-101 34304710-3 2021 Leukaemia-associated RhoGEF (LARG) is a guanine nucleotide exchange factor (GEF) that activates RhoA subfamily GTPases by promoting the exchange of GDP for GTP. Guanosine Diphosphate 148-151 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 40-74 34304710-3 2021 Leukaemia-associated RhoGEF (LARG) is a guanine nucleotide exchange factor (GEF) that activates RhoA subfamily GTPases by promoting the exchange of GDP for GTP. Guanosine Diphosphate 148-151 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 76-79 9312123-9 1997 As a consequence of this inhibition, presence of p120-GAP enhanced the regeneration of Ha-Ras.GTPgammaS by GEF at a GDP:GTPgammaS ratio mimicking the in vivo GDP:GTP ratio. Guanosine Diphosphate 116-119 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 107-110 7988425-5 1994 Insulin increased GEF activity in both fractions (48 +/- 12% [3H]GDP released vs. 24 +/- 6% in control plasma membranes, and 65 +/- 13% vs. 13 +/- 4% in control cytosolic fractions), whereas EGF enhanced only the plasma membrane-associated activity (43 +/- 12% of [3H]GDP release in the plasma membrane fraction and 10 +/- 2% in the cytosol). Guanosine Diphosphate 65-68 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 18-21 7988425-5 1994 Insulin increased GEF activity in both fractions (48 +/- 12% [3H]GDP released vs. 24 +/- 6% in control plasma membranes, and 65 +/- 13% vs. 13 +/- 4% in control cytosolic fractions), whereas EGF enhanced only the plasma membrane-associated activity (43 +/- 12% of [3H]GDP release in the plasma membrane fraction and 10 +/- 2% in the cytosol). Guanosine Diphosphate 268-271 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 18-21