PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 24470025-1 2014 Rap1 GTPases operate as molecular switches by cycling between a GDP-bound inactive state and a GTP-bound active state and regulate several cellular pathways in response to different stimuli. Guanosine Diphosphate 64-67 RAP1A, member of RAS oncogene family Homo sapiens 0-4 24704834-1 2015 SIPA1 (signal-induced proliferation-associated protein 1) is a GTPase activation protein that can catalyze the hydrolysis of Rap1 bound GTP to GDP. Guanosine Diphosphate 143-146 RAP1A, member of RAS oncogene family Homo sapiens 125-129 26634692-4 2015 Rap1-GDP associates with and activates lymphocyte-oriented kinase, which phosphorylates ERM (ezrin, radixin and moesin) in resting T cells. Guanosine Diphosphate 5-8 RAP1A, member of RAS oncogene family Homo sapiens 0-4 22614916-1 2012 Rap1GAP which regulates the GTP-GDP form switch of Rap1 is a member of the GTPase-activating protein (GAP) family and has recently received substantial attention. Guanosine Diphosphate 32-35 RAP1A, member of RAS oncogene family Homo sapiens 0-4 24349085-6 2013 Ca(2+)-activated wtTG2 cross-linked RAP1, GTP-GDP dissociation stimulator 1, an unusual guanine exchange factor acting on various small GTPases, to induce a yet uncharacterized signaling pathway that was able to promote the Ca(2+) release from the endoplasmic reticulum via both Ins3P and ryanodine sensitive receptors leading to a consequently enhanced mitochondrial Ca(2+)uptake. Guanosine Diphosphate 46-49 RAP1A, member of RAS oncogene family Homo sapiens 36-40 17300802-7 2007 Some of the mutants, however, still form a complex with Rap1*GDP in the presence of BeF(x) but not AlF(x), supporting the notion that fluoride complexes are indicators of the ground versus transition state. Guanosine Diphosphate 61-64 RAP1A, member of RAS oncogene family Homo sapiens 56-60 20413728-2 2010 CalDAG-GEFI plays a key role in the activation of beta1, beta2, and beta3 integrins in platelets and neutrophils by exchanging a GDP for a GTP on Rap1. Guanosine Diphosphate 129-132 RAP1A, member of RAS oncogene family Homo sapiens 146-150 19332557-2 2009 The bulk of Rap1 is expressed in a GDP-bound state on intracellular vesicles. Guanosine Diphosphate 35-38 RAP1A, member of RAS oncogene family Homo sapiens 12-16 20709748-5 2010 We demonstrate that insertion of the dominant negative mutation to inhibit GDP/GTP exchange diminishes prenylation of Rap1A and RhoA, enhances prenylation of Rac1, and does not detectably alter prenylation of K-Ras. Guanosine Diphosphate 75-78 RAP1A, member of RAS oncogene family Homo sapiens 118-123 20170508-7 2010 Indeed, all retrogene-encoded GTPases have an increased GTP/GDP binding ratio in vivo, indicating that their conformations resemble that of active GTP-bound Rap1. Guanosine Diphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 157-161 20124489-2 2010 Rap1GAP, a Rap1 GTPase-activating protein, inhibits the RAS superfamily protein Rap1 by facilitating hydrolysis of GTP to GDP. Guanosine Diphosphate 122-125 RAP1A, member of RAS oncogene family Homo sapiens 0-4 20124489-2 2010 Rap1GAP, a Rap1 GTPase-activating protein, inhibits the RAS superfamily protein Rap1 by facilitating hydrolysis of GTP to GDP. Guanosine Diphosphate 122-125 RAP1A, member of RAS oncogene family Homo sapiens 11-15 12082090-4 2002 However, overexpression of a RAP1 GTPase-activating protein (RAP1GAP), which efficiently clamped cellular RAP1 in the inactive GDP-bound form, did not affect A(2A)-agonist-mediated MAP kinase stimulation. Guanosine Diphosphate 127-130 RAP1A, member of RAS oncogene family Homo sapiens 29-33 10835426-2 2000 This novel GEF, referred to as CalDAG-GEFIII, increased the GTP/GDP ratio of Ha-Ras, R-Ras, and Rap1 in 293T cells. Guanosine Diphosphate 64-67 RAP1A, member of RAS oncogene family Homo sapiens 96-100 10985780-1 2000 Guanine nucleotide dissociation stimulator (GDS) promotes the release of tightly bound GDP from various Ras superfamily proteins, including RhoA, Rac1, K-Ras, Rap1A, and Rap1B. Guanosine Diphosphate 87-90 RAP1A, member of RAS oncogene family Homo sapiens 159-164 9853756-3 1998 At least three different second messengers, namely diacylglycerol, calcium and cyclic AMP, are able to activate Rap1 by promoting its release of the guanine nucleotide GDP and its binding to GTP. Guanosine Diphosphate 168-171 RAP1A, member of RAS oncogene family Homo sapiens 112-116 9882515-0 1999 RAP1A GTP/GDP cycles determine the intracellular location of the late endocytic compartments and contribute to myogenic differentiation. Guanosine Diphosphate 10-13 RAP1A, member of RAS oncogene family Homo sapiens 0-5 9882515-3 1999 By monitoring the nucleotide-bound form of RAP1A protein, we established that the various protein localizations were related to the GTP/GDP-bound state. Guanosine Diphosphate 136-139 RAP1A, member of RAS oncogene family Homo sapiens 43-48 10608844-5 1999 Hs-RA-GEF exhibited not only a GTP-dependent binding activity to Rap1A at its RA domain but also an activity to stimulate GDP/GTP exchange of Rap1A both in vitro and in vivo at the segment containing its REM and GEF domains. Guanosine Diphosphate 122-125 RAP1A, member of RAS oncogene family Homo sapiens 142-147 10608844-7 1999 On the other hand, Ce-RA-GEF associated with and stimulated GDP/GTP exchange of both Ras and Rap1A. Guanosine Diphosphate 60-63 RAP1A, member of RAS oncogene family Homo sapiens 93-98 8832375-1 1996 rap-1A is a membrane-bound G-protein in the ras superfamily that, like the ras-p21 protein, is activated by binding GTP in place of GDP. Guanosine Diphosphate 132-135 RAP1A, member of RAS oncogene family Homo sapiens 0-6 9266971-4 1997 Like the corresponding fragment from CDC25Mm, the increase in the GDP dissociation rate is linear with increasing concentration of Rap1A x GDP up to 100 microM, indicating an apparent K(M) higher than 100 microM. Guanosine Diphosphate 66-69 RAP1A, member of RAS oncogene family Homo sapiens 131-136 9266971-4 1997 Like the corresponding fragment from CDC25Mm, the increase in the GDP dissociation rate is linear with increasing concentration of Rap1A x GDP up to 100 microM, indicating an apparent K(M) higher than 100 microM. Guanosine Diphosphate 139-142 RAP1A, member of RAS oncogene family Homo sapiens 131-136 8900189-1 1996 Smg GDS is a regulator having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. Guanosine Diphosphate 156-159 RAP1A, member of RAS oncogene family Homo sapiens 98-102 8838585-1 1996 rap-1A, an anti-oncogene-encoded protein, is a ras-p21-like protein whose sequence is over 80% homologous to p21 and which interacts with the same intracellular target proteins and is activated by the same mechanisms as p21, e.g., by binding GTP in place of GDP. Guanosine Diphosphate 258-261 RAP1A, member of RAS oncogene family Homo sapiens 0-6 1763330-3 1991 The (guanosine-5"-O-(3-thiotriphosphate) (GTP-gamma-S)-bound form of Rap1A bound more tightly to cytochrome b558 than did the guanosine diphosphate-bound form. Guanosine Diphosphate 126-147 RAP1A, member of RAS oncogene family Homo sapiens 69-74 7833480-12 1995 production by the differentiated cell line expressing GTPase-defective V12 Rap1a was also significantly inhibited, a finding that is consistent with a requirement for cycling between guanosine diphosphate- and GTP-bound forms of Rap1a for continuous NADPH oxidase activation in intact neutrophils. Guanosine Diphosphate 183-204 RAP1A, member of RAS oncogene family Homo sapiens 75-80 7833480-12 1995 production by the differentiated cell line expressing GTPase-defective V12 Rap1a was also significantly inhibited, a finding that is consistent with a requirement for cycling between guanosine diphosphate- and GTP-bound forms of Rap1a for continuous NADPH oxidase activation in intact neutrophils. Guanosine Diphosphate 183-204 RAP1A, member of RAS oncogene family Homo sapiens 229-234 8034626-3 1994 Therefore, we have stably transfected human Epstein-Barr virus-transformed B lymphocytes that possess an activable NADPH oxidase complex with cDNAs for mutants of Rap1A "locked" in a GTP-bound (63E) and GDP-bound (17N) state. Guanosine Diphosphate 203-206 RAP1A, member of RAS oncogene family Homo sapiens 163-168 8034626-9 1994 Furthermore, the inhibitory effect of both GTP- as well as GDP-bound mutants indicates that Rap1A functions in a dynamic cycle as opposed to a unidirectional pathway, as is the case for the other NADPH oxidase regulatory GTP-binding protein, Rac. Guanosine Diphosphate 59-62 RAP1A, member of RAS oncogene family Homo sapiens 92-97 1939245-6 1991 This effect occurred at picomolar concentrations of Rap1A, and the GTP gamma S-bound form of the protein was consistently found to be more potent than the GDP form. Guanosine Diphosphate 155-158 RAP1A, member of RAS oncogene family Homo sapiens 52-57 2110451-4 1990 The relative affinities of GTP and GDP for the binding site on rap1 were modulated by the presence of Mg2+, with a preferential affinity (approx. Guanosine Diphosphate 35-38 RAP1A, member of RAS oncogene family Homo sapiens 63-67 1773804-3 1991 The K-rev-1 cDNA has the capacity to encode a protein with a calculated molecular weight of 21,000, having strong structural similarity to ras proteins (approximately 50% homology), especially in their guanosine triphosphate/guanosine diphosphate-binding, effector-binding, and membrane-attachment domains. Guanosine Diphosphate 225-246 RAP1A, member of RAS oncogene family Homo sapiens 4-11 2160589-3 1990 Recombinant wild-type Rap1A bound GTP gamma S, GTP, and GDP with affinities similar to those observed for neutrophil Rap1 protein. Guanosine Diphosphate 56-59 RAP1A, member of RAS oncogene family Homo sapiens 22-27 2160589-3 1990 Recombinant wild-type Rap1A bound GTP gamma S, GTP, and GDP with affinities similar to those observed for neutrophil Rap1 protein. Guanosine Diphosphate 56-59 RAP1A, member of RAS oncogene family Homo sapiens 22-26 2110451-6 1990 The dissociation of GDP from rap1 was not affected by the G-protein beta/gamma-subunit complex. Guanosine Diphosphate 20-23 RAP1A, member of RAS oncogene family Homo sapiens 29-33 35565390-6 2022 More precisely, TRPM8 inhibits cell migration and adhesion by trapping Rap1A in its GDP-bound inactive form, thus preventing its activation at the plasma membrane. Guanosine Diphosphate 84-87 RAP1A, member of RAS oncogene family Homo sapiens 71-76 29337043-8 2018 Geranylgeranylated TAT-Rab27A-GTP-gamma-S promoted the exchange of GDP for GTP on Rab3 and Rap1 detected by far-immunofluorescence with Rab3-GTP and Rap1-GTP binding cassettes. Guanosine Diphosphate 67-70 RAP1A, member of RAS oncogene family Homo sapiens 91-95 29337043-8 2018 Geranylgeranylated TAT-Rab27A-GTP-gamma-S promoted the exchange of GDP for GTP on Rab3 and Rap1 detected by far-immunofluorescence with Rab3-GTP and Rap1-GTP binding cassettes. Guanosine Diphosphate 67-70 RAP1A, member of RAS oncogene family Homo sapiens 149-153