PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
10724483-8	2000	Although RAS2 is not involved in the regulation of SRP1 expression, the guanine nucleotide exchange factor Cdc25, which is known to control the GTP/GDP ratio on the Ras proteins, was nevertheless required for hypoxic SRP1 expression.	Guanosine Diphosphate	148-151	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	107-112	
22818197-5	2012	Then, we show the results on cAMP oscillations when perturbing the amount of protein Cdc25 coupled with the alteration of the intracellular ratio of the guanine nucleotides (GTP/GDP), which are known to regulate the switch of the GTPase Ras protein.	Guanosine Diphosphate	178-181	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	85-90	
17134721-4	2007	With a K(d) value for the Sec2:Sec4.GDP interaction of ca 70 microM and a maximal rate of GDP displacement of ca 15 s(-1), it is 100-1000-fold more effective than other proteins showing exchange activity towards Rabs (MSS4, DSS4, Vps9) and ca tenfold faster than Cdc25 as a Ras specific exchanger, although still 100-fold slower than the fastest systems studied so far, EF-Tu/Ef-Ts and Ran/RCC1.	Guanosine Diphosphate	36-39	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	263-268	
17134721-4	2007	With a K(d) value for the Sec2:Sec4.GDP interaction of ca 70 microM and a maximal rate of GDP displacement of ca 15 s(-1), it is 100-1000-fold more effective than other proteins showing exchange activity towards Rabs (MSS4, DSS4, Vps9) and ca tenfold faster than Cdc25 as a Ras specific exchanger, although still 100-fold slower than the fastest systems studied so far, EF-Tu/Ef-Ts and Ran/RCC1.	Guanosine Diphosphate	90-93	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	263-268	
10747953-2	2000	We have analyzed the function of this region and the effect of its farnesylation with respect to the action of the GDP/GTP exchange factors (GEFs) Cdc25p and Sdc25p and the target adenylyl cyclase.	Guanosine Diphosphate	115-118	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	147-153	
8441380-5	1993	The CDC25 fusion protein catalyzed replacement of GDP-bound to Ras2 with GTP (activation) more efficiently than that of the reverse reaction of replacement of GTP for GDP (deactivation), consistent with prior genetic analysis of CDC25 which indicated a positive role in the activation of Ras.	Guanosine Diphosphate	50-53	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	4-9	
7577970-7	1995	The simultaneous presence of the catalytic domains of Ira2p and the yeast GDP/GTP exchange factor Cdc25p induced on Ras2p a multiple-round reaction of GTP hydrolysis and GDP/GTP exchange, showing that it is possible to reconstitute in vitro a S. cerevisiae system suitable for the study of the regulation of the Ras2p GDP/GTP cycle.	Guanosine Diphosphate	74-77	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	98-104	
7577970-7	1995	The simultaneous presence of the catalytic domains of Ira2p and the yeast GDP/GTP exchange factor Cdc25p induced on Ras2p a multiple-round reaction of GTP hydrolysis and GDP/GTP exchange, showing that it is possible to reconstitute in vitro a S. cerevisiae system suitable for the study of the regulation of the Ras2p GDP/GTP cycle.	Guanosine Diphosphate	170-173	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	98-104	
7641877-9	1995	A positive regulation of RAS is carried out by CDC25 product which facilitates the GDP/GTP exchange on RAS proteins.	Guanosine Diphosphate	83-86	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	47-52	
8206969-4	1994	Using Ras2p and a catalytic fragment of Cdc25p, both expressed in and purified from Escherichia coli, we determined that Cdc25p has a Km for Ras2p-GDP of 160 nM and a maximal rate of 0.20 s-1.	Guanosine Diphosphate	147-150	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	121-127	
8206969-5	1994	The Km of Cdc25p for Ras2p complexed to GTP is 3-fold greater than that for Ras2p complexed to GDP.	Guanosine Diphosphate	95-98	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	10-16	
8289791-8	1994	Most critically, a complex of Ras and CDC25 protein, unlike free Fas protein, possesses significantly greater affinity for GTP than for GDP.	Guanosine Diphosphate	136-139	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	38-43	
8289791-9	1994	Furthermore, the Ras CDC25 complex is more readily dissociated into free subunits by GTP than it is by GDP.	Guanosine Diphosphate	103-106	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	21-26	
8289791-10	1994	Both of these results suggest a function for CDC25 in promoting the selective exchange of GTP for GDP.	Guanosine Diphosphate	98-101	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	45-50	
8371782-4	1993	Bud1 protein (Bud1p) has sequence similarity to Ras, a small GTP-binding protein, and Bud5p is similar to Cdc25p (refs 4, 5), a GDP-GTP exchange factor.	Guanosine Diphosphate	128-131	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	106-112	
8441380-8	1993	This higher affinity of CDC25 for the nucleotide-free form than for either the GDP- or GTP-bound form suggests that CDC25 catalyzes exchange of guanine nucleotides bound to Ras proteins by stabilization of the transitory nucleotide-free state.	Guanosine Diphosphate	79-82	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	24-29	
8441380-8	1993	This higher affinity of CDC25 for the nucleotide-free form than for either the GDP- or GTP-bound form suggests that CDC25 catalyzes exchange of guanine nucleotides bound to Ras proteins by stabilization of the transitory nucleotide-free state.	Guanosine Diphosphate	79-82	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	116-121	
8441380-5	1993	The CDC25 fusion protein catalyzed replacement of GDP-bound to Ras2 with GTP (activation) more efficiently than that of the reverse reaction of replacement of GTP for GDP (deactivation), consistent with prior genetic analysis of CDC25 which indicated a positive role in the activation of Ras.	Guanosine Diphosphate	50-53	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	229-234	
1447167-8	1992	Our results show that a cloned GDP to GTP exchange factor of mammalian ras belongs to the novel family of CDC25-like proteins.	Guanosine Diphosphate	31-34	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	106-111	
1379731-1	1992	The yeast Saccharomyces cerevisiae CDC25 gene encodes a nucleotide-exchange-factor (NEF) that can convert the inactive GDP-bound state of RAS proteins to an active RAS-GTP complex.	Guanosine Diphosphate	119-122	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	35-40	
1901951-8	1991	smg p21 GDS has low amino acid sequence homology with the yeast CDC25 and SCD25 proteins, which may regulate the GDP/GTP exchange reaction of the yeast RAS2 protein, but not with ras p21 GTPase-activating protein, the inhibitory GDP/GTP exchange proteins (GDP dissociation inhibitor) for smg p25A and rho p21s, and the beta gamma subunits of heterotrimeric GTP-binding proteins such as Gs and Gi.	Guanosine Diphosphate	113-116	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	64-69	
1569942-6	1992	Cdc25 binds predominantly to the catalytically inactive GDP-bound form of Ras2, whereas a conformational change of Ras2 to its activated GTP-bound state results in its loss of binding affinity to Cdc25.	Guanosine Diphosphate	56-59	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	0-5	
2017169-3	1991	Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras.	Guanosine Diphosphate	78-81	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	46-52	
1901951-8	1991	smg p21 GDS has low amino acid sequence homology with the yeast CDC25 and SCD25 proteins, which may regulate the GDP/GTP exchange reaction of the yeast RAS2 protein, but not with ras p21 GTPase-activating protein, the inhibitory GDP/GTP exchange proteins (GDP dissociation inhibitor) for smg p25A and rho p21s, and the beta gamma subunits of heterotrimeric GTP-binding proteins such as Gs and Gi.	Guanosine Diphosphate	229-232	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	64-69	
1901951-8	1991	smg p21 GDS has low amino acid sequence homology with the yeast CDC25 and SCD25 proteins, which may regulate the GDP/GTP exchange reaction of the yeast RAS2 protein, but not with ras p21 GTPase-activating protein, the inhibitory GDP/GTP exchange proteins (GDP dissociation inhibitor) for smg p25A and rho p21s, and the beta gamma subunits of heterotrimeric GTP-binding proteins such as Gs and Gi.	Guanosine Diphosphate	229-232	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	64-69	
2115118-8	1990	smg p25A GDI shares low amino acid sequence homology with the Saccharomyces cerevisiae CDC25-encoded protein, which has been suggested to serve as a factor that regulates the GDP-GTP exchange reaction of the yeast RAS2-encoded protein, but not with the beta gamma subunits of GTP-binding proteins having an alpha beta gamma subunit structure, such as Gs and Gi.	Guanosine Diphosphate	175-178	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	87-92	
2104846-6	1990	Our kinetic results show that the functional modifications of RAS2 compensating for the CDC25 inactivation can not only be associated with the presence of a long-lived RAS2.GTP complex, but also with a rapid GDP to GTP exchange reaction.	Guanosine Diphosphate	208-211	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	88-93	
2547147-5	1989	Stimulation of adenylyl cyclase is dependent not only on RAS protein complexed to GTP, but also on the CDC25 and IRA gene products, which appear to control the RAS GTP-guanosine diphosphate cycle.	Guanosine Diphosphate	168-189	Ras family guanine nucleotide exchange factor CDC25	Saccharomyces cerevisiae S288C	103-108