PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1618793-4 1992 In contrast, when 60 S ribosomal subunits were also present in an eIF-5-catalyzed reaction, the eIF-2.GDP produced remained bound to the 60 S ribosomal subunit of the 80 S initiation complex. Guanosine Diphosphate 102-105 eukaryotic translation initiation factor 5 Homo sapiens 66-71 3711104-2 1986 Incubation of a 40 S initiation complex with eIF-5, in the presence or absence of 60 S ribosomal subunits at 25 degrees C, causes rapid and quantitative hydrolysis of ribosome-bound GTP to form an eIF-2.GDP binary complex and Pi. Guanosine Diphosphate 203-206 eukaryotic translation initiation factor 5 Homo sapiens 45-50 3711104-1 1986 Studies on the formation and release of the eukaryotic initiation factor (eIF)-2.GDP binary complex formed during eIF-5-mediated assembly of an 80 S initiation complex have been carried out. Guanosine Diphosphate 81-84 eukaryotic translation initiation factor 5 Homo sapiens 114-119 3711104-4 1986 The eIF-2.GDP binary complex can however be released from ribosome by subjecting the eIF-5-catalyzed reaction products to either longer periods of incubation at 37 degrees C or sucrose gradient centrifugation. Guanosine Diphosphate 10-13 eukaryotic translation initiation factor 5 Homo sapiens 85-90 27458202-0 2016 eIF2beta is critical for eIF5-mediated GDP-dissociation inhibitor activity and translational control. Guanosine Diphosphate 39-42 eukaryotic translation initiation factor 5 Homo sapiens 25-29 3844406-4 1985 Evidence is presented that eIF-5-mediated hydrolysis releases the GTP bound to the 40 S initiation complex as an intact eIF-2 X GDP complex rather than as free GDP and eIF-2 which subsequently recombine to form the binary complex. Guanosine Diphosphate 128-131 eukaryotic translation initiation factor 5 Homo sapiens 27-32 3844406-4 1985 Evidence is presented that eIF-5-mediated hydrolysis releases the GTP bound to the 40 S initiation complex as an intact eIF-2 X GDP complex rather than as free GDP and eIF-2 which subsequently recombine to form the binary complex. Guanosine Diphosphate 160-163 eukaryotic translation initiation factor 5 Homo sapiens 27-32 3844406-5 1985 Furthermore, formation and release of eIF-2 X GDP from the ribosomal complex do not require concomitant formation of an 80 S initiation complex since both reactions occur efficiently when the 40 S initiation complex reacts with eIF-5 in the absence of 60 S ribosomal subunits. Guanosine Diphosphate 46-49 eukaryotic translation initiation factor 5 Homo sapiens 228-233 30211544-4 2018 Upon start codon selection and GTP hydrolysis promoted by the GTPase-activating protein (GAP) eIF5, eIF2-GDP is displaced from Met-tRNAi by eIF5B-GTP and is released in complex with eIF5. Guanosine Diphosphate 105-108 eukaryotic translation initiation factor 5 Homo sapiens 94-98 30211544-4 2018 Upon start codon selection and GTP hydrolysis promoted by the GTPase-activating protein (GAP) eIF5, eIF2-GDP is displaced from Met-tRNAi by eIF5B-GTP and is released in complex with eIF5. Guanosine Diphosphate 105-108 eukaryotic translation initiation factor 5 Homo sapiens 140-144 30211544-11 2018 Our results indicate that in humans, eIF5B displacing eIF2 from Met-tRNAi upon subunit joining may be coupled to eIF1A displacing eIF5 from eIF5B, allowing the eIF5:eIF2-GDP complex to leave the ribosome. Guanosine Diphosphate 170-173 eukaryotic translation initiation factor 5 Homo sapiens 37-41 30211544-11 2018 Our results indicate that in humans, eIF5B displacing eIF2 from Met-tRNAi upon subunit joining may be coupled to eIF1A displacing eIF5 from eIF5B, allowing the eIF5:eIF2-GDP complex to leave the ribosome. Guanosine Diphosphate 170-173 eukaryotic translation initiation factor 5 Homo sapiens 130-134 29425030-6 2018 The available evidence leads to the conclusion that eIF2 is channeled from the ribosome (as an eIF5 eIF2-GDP complex) to eIF2B, converted by eIF2B to the TC, which is then channeled back to eIF5 and the ribosome. Guanosine Diphosphate 105-108 eukaryotic translation initiation factor 5 Homo sapiens 190-194 3844406-1 1985 The formation and release of an eukaryotic initiation factor (eIF)-2 X GDP binary complex during eIF-5-mediated assembly of an 80 S ribosomal polypeptide chain initiation complex have been studied by sucrose gradient centrifugation analysis. Guanosine Diphosphate 71-74 eukaryotic translation initiation factor 5 Homo sapiens 97-102 3844406-2 1985 Isolated 40 S initiation complex reacts with eIF-5 and 60 S ribosomal subunits to form an 80 S ribosomal initiation complex with concomitant hydrolysis of an equimolar amount of bound GTP to GDP and Pi. Guanosine Diphosphate 191-194 eukaryotic translation initiation factor 5 Homo sapiens 45-50 29425030-6 2018 The available evidence leads to the conclusion that eIF2 is channeled from the ribosome (as an eIF5 eIF2-GDP complex) to eIF2B, converted by eIF2B to the TC, which is then channeled back to eIF5 and the ribosome. Guanosine Diphosphate 105-108 eukaryotic translation initiation factor 5 Homo sapiens 95-99 28315520-3 2017 Key to controlling the activity of eIF2 are translation factors eIF2B and eIF5, thought to primarily function with eIF2-GDP and TC respectively. Guanosine Diphosphate 120-123 eukaryotic translation initiation factor 5 Homo sapiens 74-78 27458202-5 2016 Instead we show that the eIF2beta mutation prevents eIF5 GDI stabilizing nucleotide binding to eIF2, thereby altering the off-rate of GDP from eIF2 GDP/eIF5 complexes. Guanosine Diphosphate 134-137 eukaryotic translation initiation factor 5 Homo sapiens 52-56 27458202-5 2016 Instead we show that the eIF2beta mutation prevents eIF5 GDI stabilizing nucleotide binding to eIF2, thereby altering the off-rate of GDP from eIF2 GDP/eIF5 complexes. Guanosine Diphosphate 134-137 eukaryotic translation initiation factor 5 Homo sapiens 152-156 27458202-7 2016 These findings provide support for the importance of eIF5 GDI activity in vivo and demonstrate that eIF2beta acts in concert with eIF5 to prevent premature release of GDP from eIF2gamma and thereby ensure tight control of protein synthesis initiation. Guanosine Diphosphate 167-170 eukaryotic translation initiation factor 5 Homo sapiens 130-134 24352424-0 2013 eIF2B promotes eIF5 dissociation from eIF2*GDP to facilitate guanine nucleotide exchange for translation initiation. Guanosine Diphosphate 43-46 eukaryotic translation initiation factor 5 Homo sapiens 15-19 24352424-2 2013 Two proteins regulate its G-protein cycle: eIF5 has both GTPase-accelerating protein (GAP) and GDP dissociation inhibitor (GDI) functions, and eIF2B is the guanine nucleotide exchange factor (GEF). Guanosine Diphosphate 95-98 eukaryotic translation initiation factor 5 Homo sapiens 43-47 23293029-5 2013 The data further indicate that eIF1 dissociation must be accompanied by the movement of the eIF1A CTT toward eIF5 in order to trigger release of phosphate from eIF2, which converts the latter to its GDP-bound state. Guanosine Diphosphate 199-202 eukaryotic translation initiation factor 5 Homo sapiens 109-113 20485439-4 2010 Here we define new regulatory functions of eIF5 in the recycling of eIF2 from its inactive eIF2.GDP state between successive rounds of translation initiation. Guanosine Diphosphate 96-99 eukaryotic translation initiation factor 5 Homo sapiens 43-47 20485439-5 2010 First we show that eIF5 stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). Guanosine Diphosphate 50-53 eukaryotic translation initiation factor 5 Homo sapiens 19-23 20485439-5 2010 First we show that eIF5 stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). Guanosine Diphosphate 118-121 eukaryotic translation initiation factor 5 Homo sapiens 19-23 17255934-2 2007 Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. Guanosine Diphosphate 178-181 eukaryotic translation initiation factor 5 Homo sapiens 141-145 15601822-2 2004 We report that eIF5-induced hydrolysis of eIF2-bound GTP in 48S complexes led to release of eIF2-GDP but not eIF3 or eIF1. Guanosine Diphosphate 97-100 eukaryotic translation initiation factor 5 Homo sapiens 15-19 21686265-1 2010 We recently showed in a publication in Nature that the eukaryotic translation initiation factor eIF5 has a second regulatory function and is a GDI (GDP dissociation inhibitor) in addition to its previously characterized role as a GAP (GTPase accelerating protein). Guanosine Diphosphate 148-151 eukaryotic translation initiation factor 5 Homo sapiens 96-100 16990799-1 2006 In eukaryotic translation initiation, the eIF2.GTP/Met-tRNA(i)(Met) ternary complex (TC) binds the eIF3/eIF1/eIF5 complex to form the multifactor complex (MFC), whereas eIF2.GDP binds the pentameric factor eIF2B for guanine nucleotide exchange. Guanosine Diphosphate 174-177 eukaryotic translation initiation factor 5 Homo sapiens 109-113