PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33734638-6 2021 Prolactin level was measured by the immunoenzyme method with manual PEG precipitation and TRACE. Polyethylene Glycols 68-71 prolactin Homo sapiens 0-9 34620143-2 2021 We aimed to determine the frequency of macroprolactinemia in Chinese hyperprolactinemic patients using monomeric prolactin discriminated by precipitation with polyethylene glycol (PEG). Polyethylene Glycols 159-178 prolactin Homo sapiens 113-122 34620143-2 2021 We aimed to determine the frequency of macroprolactinemia in Chinese hyperprolactinemic patients using monomeric prolactin discriminated by precipitation with polyethylene glycol (PEG). Polyethylene Glycols 180-183 prolactin Homo sapiens 113-122 35232561-11 2022 To create a consensus document that standardizes the reporting of prolactin results after precipitation with PEG to minimize errors in the interpretation of the results, in line with international standards. Polyethylene Glycols 109-112 prolactin Homo sapiens 66-75 943720-4 1976 3) Quantitative determination on PEG according to our own method showed that tumor size of these patients with abnormally high plasma prolactin level was larger than that of relatively lower plasma prolactin level. Polyethylene Glycols 33-36 prolactin Homo sapiens 134-143 943720-4 1976 3) Quantitative determination on PEG according to our own method showed that tumor size of these patients with abnormally high plasma prolactin level was larger than that of relatively lower plasma prolactin level. Polyethylene Glycols 33-36 prolactin Homo sapiens 198-207 33734638-12 2021 Measurements of prolactin levels by the TRACE method is useful for correct diagnosis in patients with equivocal results received by traditional method with PEG precipitation. Polyethylene Glycols 156-159 prolactin Homo sapiens 16-25 32597541-3 2020 METHODS: From January to July in 2018, totally 317 patients with elevated PRL were subjected to the polyethylene glycol (PEG) precipitation assay. Polyethylene Glycols 100-119 prolactin Homo sapiens 74-77 29333150-2 2017 Poly ethylene glycol (PEG) pretreatment is the preventive process but such process includes the probability of loss of a fraction of bioactive prolactin. Polyethylene Glycols 0-20 prolactin Homo sapiens 143-152 32550971-4 2020 Recovery of less than 40% of serum prolactin after polyethylene glycol (PEG) precipitation was indicative of macroprolactinemia. Polyethylene Glycols 51-70 prolactin Homo sapiens 35-44 32550971-4 2020 Recovery of less than 40% of serum prolactin after polyethylene glycol (PEG) precipitation was indicative of macroprolactinemia. Polyethylene Glycols 72-75 prolactin Homo sapiens 35-44 30072818-10 2018 Measurement of prolactin post polyethylene glycol precipitation (PEG) when prolactin levels are above the reference interval is routinely used to identify macroprolactin, however harmonisation of PEG precipitation process and reporting may improve clinical care. Polyethylene Glycols 30-49 prolactin Homo sapiens 15-24 30072818-10 2018 Measurement of prolactin post polyethylene glycol precipitation (PEG) when prolactin levels are above the reference interval is routinely used to identify macroprolactin, however harmonisation of PEG precipitation process and reporting may improve clinical care. Polyethylene Glycols 30-49 prolactin Homo sapiens 75-84 30072818-10 2018 Measurement of prolactin post polyethylene glycol precipitation (PEG) when prolactin levels are above the reference interval is routinely used to identify macroprolactin, however harmonisation of PEG precipitation process and reporting may improve clinical care. Polyethylene Glycols 65-68 prolactin Homo sapiens 15-24 30072818-10 2018 Measurement of prolactin post polyethylene glycol precipitation (PEG) when prolactin levels are above the reference interval is routinely used to identify macroprolactin, however harmonisation of PEG precipitation process and reporting may improve clinical care. Polyethylene Glycols 65-68 prolactin Homo sapiens 75-84 29333150-2 2017 Poly ethylene glycol (PEG) pretreatment is the preventive process but such process includes the probability of loss of a fraction of bioactive prolactin. Polyethylene Glycols 22-25 prolactin Homo sapiens 143-152 25074991-8 2015 Mean (SD) post-PEG percentage recovery of the IS 84/500 prolactin standard was 80 (2.3)%. Polyethylene Glycols 15-18 prolactin Homo sapiens 56-65 26398376-5 2015 RESULTS: Oral contraceptive pills administered for 16 weeks slightly increased pre-polyethylene glycol serum prolactin levels and macroprolactin levels and the effect of this treatment correlated with their baseline values. Polyethylene Glycols 83-102 prolactin Homo sapiens 109-118 27212171-0 2016 A new criteria for screening macroprolactinemia using polyethylene glycol treatment combined with different assays for prolactin. Polyethylene Glycols 54-73 prolactin Homo sapiens 34-43 27212171-15 2016 CONCLUSIONS: Establishment of different criteria for screening MP by using PEG-method is helpful for the accuracy of PRL determination and its comparability. Polyethylene Glycols 75-78 prolactin Homo sapiens 117-120 23817395-13 2013 CONCLUSION: Macroprolactin is a significant cause of misdiagnosis, unnecessary investigation, and inappropriate treatment and hence it is useful to screen all patients with high PRL levels with PEG precipitation and to apply GFC to samples with recoveries <50%. Polyethylene Glycols 194-197 prolactin Homo sapiens 178-181 25461348-1 2014 Macroprolactin is an antigen-antibody complex of higher molecular mass than prolactin (>150kDa), consisting of monomeric prolactin and immunoglobulin G. The term "macroprolactinemia" is used when the concentration of macroprolactin exceeds 60% of the total serum prolactin concentration determined by polyethylene glycol precipitation. Polyethylene Glycols 304-323 prolactin Homo sapiens 5-14 24903198-9 2014 Macroprolactinemia was excluded by the precipitation of serum with polyethylene glycol in patients with increased prolactin levels. Polyethylene Glycols 67-86 prolactin Homo sapiens 5-14 24782376-1 2014 OBJECTIVE: To establish a polyethylene glycol (PEG6000) precipitation method for screening macroprolactinemia in patients with high serum prolactin (PRL). Polyethylene Glycols 26-45 prolactin Homo sapiens 96-105 24533748-6 2014 In case of coexistence of macroprolactinaemia and raised free PRL after PEG precipitation of macroprolactin, the chance of finding of either a micro- or a macroadenoma was 36% (13 cases out of 36). Polyethylene Glycols 72-75 prolactin Homo sapiens 62-65 24533748-8 2014 Furthermore, if free PRL is raised after PEG precipitation of macroprolactin, then the chance of finding either a pituitary micro- or macroadenoma in women with oligo-/amenorrhoea is over 30%. Polyethylene Glycols 41-44 prolactin Homo sapiens 21-24 24194758-13 2013 Serum PRL precipitated with PEG is a convenient and simple procedure to screen for the presence of macroprolactinemia. Polyethylene Glycols 28-31 prolactin Homo sapiens 6-9 22247016-8 2012 The ratios of PEG-precipitable PRL and IgG-bound PRL did not significantly change, but (125)I-PRL binding ratios significantly increased. Polyethylene Glycols 14-17 prolactin Homo sapiens 31-34 23244563-8 2012 Raised Prl concentrations were found in 10 women with thyroid disease (5.5%), and of those a significant macroprolactinaemia (i.e., reduction of Prl concentrations of more than 60% after PEG precipitation) was found in 9 subjects (4.94%). Polyethylene Glycols 187-190 prolactin Homo sapiens 7-10 22619354-1 2012 BACKGROUND: When screening for macroprolactin, many laboratories use precipitation by polyethylene glycol (PEG) with molecular weight 6000 (PEG6000) or 8000 (PEG8000), and report the percentage prolactin recovery. Polyethylene Glycols 86-105 prolactin Homo sapiens 36-45 22619354-1 2012 BACKGROUND: When screening for macroprolactin, many laboratories use precipitation by polyethylene glycol (PEG) with molecular weight 6000 (PEG6000) or 8000 (PEG8000), and report the percentage prolactin recovery. Polyethylene Glycols 107-110 prolactin Homo sapiens 36-45 22863502-5 2011 Polyetilenoglycol (PEG) is the most used method that removes PRL from serum. Polyethylene Glycols 19-22 prolactin Homo sapiens 61-64 18482912-2 2008 We report two cases of hyperprolactinaemia in which a low recovery of serum prolactin (PRL) after PEG precipitation indicated the presence of macroprolactin, but no macroprolactin was detected by gel filtration chromatography (GFC). Polyethylene Glycols 98-101 prolactin Homo sapiens 87-90 22144220-4 2011 The most commonly used technique to separate the isoforms of PRL is precipitation with polyethylene glycol (PEG). Polyethylene Glycols 87-106 prolactin Homo sapiens 61-64 22144220-4 2011 The most commonly used technique to separate the isoforms of PRL is precipitation with polyethylene glycol (PEG). Polyethylene Glycols 108-111 prolactin Homo sapiens 61-64 19943807-4 2010 The goal of this study was to determine the need to continue PEG precipitation when prolactin measurements with the Wallac AutoDELFIA were replaced by the Beckman DxI 800. Polyethylene Glycols 61-64 prolactin Homo sapiens 84-93 20874771-3 2010 We also investigated whether we could predict the composition of macroprolactin based on the ratio of polyethylene glycol (PEG)-precipitable PRL. Polyethylene Glycols 123-126 prolactin Homo sapiens 141-144 20874771-6 2010 Macroprolactinaemia was defined by PEG-precipitable PRL ratio greater than 60%. Polyethylene Glycols 35-38 prolactin Homo sapiens 52-55 20874771-10 2010 A non-IgG-bound form of macroprolactin was found mainly in sera with marginally elevated PEG-precipitable PRL. Polyethylene Glycols 89-92 prolactin Homo sapiens 106-109 20874771-11 2010 The higher the PEG-precipitable PRL ratio, the greater the likelihood that autoantibodies were involved in the composition of macroprolactin. Polyethylene Glycols 15-18 prolactin Homo sapiens 32-35 20874771-13 2010 Long-term follow-up (2-17 years) revealed that the ratios of PEG-precipitable PRL, IgG-bound PRL and anti-PRL autoantibody-bound PRL were relatively stable. Polyethylene Glycols 61-64 prolactin Homo sapiens 78-81 20874771-14 2010 CONCLUSIONS: This study showed that higher PEG-precipitable PRL ratio in macroprolactinaemic sera might preferentially indicate the presence of anti-PRL autoantibodies and that macroprolactinaemia might be a long-lasting condition. Polyethylene Glycols 43-46 prolactin Homo sapiens 60-63 20874771-14 2010 CONCLUSIONS: This study showed that higher PEG-precipitable PRL ratio in macroprolactinaemic sera might preferentially indicate the presence of anti-PRL autoantibodies and that macroprolactinaemia might be a long-lasting condition. Polyethylene Glycols 43-46 prolactin Homo sapiens 149-152 20568397-10 2010 Prolactin biological activity was estimated by using polyethylene glycol (PEG) method. Polyethylene Glycols 53-72 prolactin Homo sapiens 0-9 20568397-10 2010 Prolactin biological activity was estimated by using polyethylene glycol (PEG) method. Polyethylene Glycols 74-77 prolactin Homo sapiens 0-9 18482912-4 2008 METHODS: The effect of increasing concentrations of gamma globulin on the precipitation of PRL by PEG was studied by adding purified human gamma globulin to serum. Polyethylene Glycols 98-101 prolactin Homo sapiens 91-94 18482912-6 2008 RESULTS: Addition of gamma globulin decreased the recovery of PRL following precipitation with PEG and gamma globulin concentrations correlated inversely with PRL concentrations (r = 0.9429, P < 0.0167) and percentage recovery of PRL (r = -1.000, P < 0.005). Polyethylene Glycols 95-98 prolactin Homo sapiens 62-65 18482912-7 2008 Only one out of 10 samples from HIV-infected patients with PRL recoveries of <60% following PEG precipitation showed a substantial macroprolactin component on GFC. Polyethylene Glycols 95-98 prolactin Homo sapiens 59-62 18482912-8 2008 CONCLUSIONS: Monomeric PRL is co-precipitated with serum globulins by PEG. Polyethylene Glycols 70-73 prolactin Homo sapiens 23-26 18482912-9 2008 Increased serum globulin concentrations can increase the amount of monomeric PRL precipitated by PEG giving a false estimate of the monomeric PRL and the erroneous impression that macroprolactin is present. Polyethylene Glycols 97-100 prolactin Homo sapiens 77-80 17027953-9 2007 In contrast, recovery of prolactin in samples without macroprolactin showed a considerable disagreement between ultrafiltration and PEG precipitation (r(s)=0.48). Polyethylene Glycols 132-135 prolactin Homo sapiens 25-34 15811931-5 2005 Application of an absolute prolactin threshold after polyethylene glycol treatment of sera, rather than the traditional method, i.e. less than 40% recovery, minimizes the opportunity for misclassification of patients in whom macroprolactin accounted for more than 60% of prolactin and the residual bioactive prolactin was present in excess. Polyethylene Glycols 53-72 prolactin Homo sapiens 27-36 16919253-3 2006 Macro-PRL was assessed from (1) percent PRL recovery, using cut-off values derived by gel filtration chromatography (GFC) and (2) significant (p<0.05) normalisation of PRL following PEG. Polyethylene Glycols 185-188 prolactin Homo sapiens 6-9 16919253-6 2006 The total PRL normalised following PEG in 7.4%. Polyethylene Glycols 35-38 prolactin Homo sapiens 10-13 16919253-9 2006 Regression analysis suggested that PEG precipitated both macro-PRL and big-PRL. Polyethylene Glycols 35-38 prolactin Homo sapiens 63-66 16919253-9 2006 Regression analysis suggested that PEG precipitated both macro-PRL and big-PRL. Polyethylene Glycols 35-38 prolactin Homo sapiens 75-78 16919253-11 2006 Screening using PEG is applicable to assays with low macro-PRL reactivity provided specific reference values are derived. Polyethylene Glycols 16-19 prolactin Homo sapiens 59-62 12668823-5 2003 Repeat prolactin measurement after polyethylene glycol precipitation showed that the majority of circulating prolactin was macroprolactin. Polyethylene Glycols 35-54 prolactin Homo sapiens 109-118 12928232-8 2003 For macroprolactinemic samples, PEG treatment decreased mean (SD) prolactin from 1524 (202) mIU/L to 202 (27) mIU/L but decreased it only from 2096 (233) mIU/L to 1705 (190) mIU/L in true hyperprolactinemic patients (P <0.01 between groups). Polyethylene Glycols 32-35 prolactin Homo sapiens 9-18 12928232-13 2003 The use of an appropriate reference interval for the PEG immunoprecipitation procedure may be of particular importance in those patients who have an excess of both macroprolactin and monomeric prolactin. Polyethylene Glycols 53-56 prolactin Homo sapiens 169-178 12668823-7 2003 Polyethylene glycol study showed that macroprolactinaemia exists simultaneously with genuine hyperprolactinaemia leading to falsely high serum prolactin levels. Polyethylene Glycols 0-19 prolactin Homo sapiens 43-52 11847468-17 2001 As macroprolactin seems to have minimal clinical relevance, it would be important that the users of PRL assays be aware to what extent macroprolactin interferes with their assays, and have available a validated method, such as the PEG precipitation test, to confirm the presence of macroprolactin. Polyethylene Glycols 231-234 prolactin Homo sapiens 100-103 12111188-5 2002 The presence of anti-prolactin auto-antibodies was suspected because of low recovery of PRL after precipitation with polyethylene glycol and confirmed by immunoprecipitation with anti-human IgG-agarose. Polyethylene Glycols 117-136 prolactin Homo sapiens 21-30 11897686-7 2002 In solution with polyethylene glycol, precipitation occurred with acidic pH, precipitation with Zn(2+) occurred effectively at acidic pH, and the ratio of Zn(2+) to PRL was less than 1. Polyethylene Glycols 17-36 prolactin Homo sapiens 165-168 11158068-3 2001 Sera from 8 of the 209 women (3.8%) were found to have a significantly high proportion of precipitated PRL by PEG (macroprolactinemia); in these patients, gel filtration showed that a substantial amount of big big PRL (molecular mass >100 kDa) was present (19.0--78.2% vs. 3.8-4.9%, P = 0.009 in normal pregnant women with a normal proportion of precipitated PRL by PEG). Polyethylene Glycols 110-113 prolactin Homo sapiens 103-106 11158068-3 2001 Sera from 8 of the 209 women (3.8%) were found to have a significantly high proportion of precipitated PRL by PEG (macroprolactinemia); in these patients, gel filtration showed that a substantial amount of big big PRL (molecular mass >100 kDa) was present (19.0--78.2% vs. 3.8-4.9%, P = 0.009 in normal pregnant women with a normal proportion of precipitated PRL by PEG). Polyethylene Glycols 369-372 prolactin Homo sapiens 103-106 11158068-3 2001 Sera from 8 of the 209 women (3.8%) were found to have a significantly high proportion of precipitated PRL by PEG (macroprolactinemia); in these patients, gel filtration showed that a substantial amount of big big PRL (molecular mass >100 kDa) was present (19.0--78.2% vs. 3.8-4.9%, P = 0.009 in normal pregnant women with a normal proportion of precipitated PRL by PEG). Polyethylene Glycols 369-372 prolactin Homo sapiens 214-217 11158068-3 2001 Sera from 8 of the 209 women (3.8%) were found to have a significantly high proportion of precipitated PRL by PEG (macroprolactinemia); in these patients, gel filtration showed that a substantial amount of big big PRL (molecular mass >100 kDa) was present (19.0--78.2% vs. 3.8-4.9%, P = 0.009 in normal pregnant women with a normal proportion of precipitated PRL by PEG). Polyethylene Glycols 369-372 prolactin Homo sapiens 214-217 11403264-1 2001 The objective of this study was to determine the diagnostic performance of the percentage of serum prolactin (PRL) precipitated with polyethylene glycol (PEG) for the detection of macroprolactinemia in systemic lupus erythematosus (SLE) patients with hyperprolactinemia. Polyethylene Glycols 133-152 prolactin Homo sapiens 99-108 11403264-1 2001 The objective of this study was to determine the diagnostic performance of the percentage of serum prolactin (PRL) precipitated with polyethylene glycol (PEG) for the detection of macroprolactinemia in systemic lupus erythematosus (SLE) patients with hyperprolactinemia. Polyethylene Glycols 133-152 prolactin Homo sapiens 110-113 11403264-1 2001 The objective of this study was to determine the diagnostic performance of the percentage of serum prolactin (PRL) precipitated with polyethylene glycol (PEG) for the detection of macroprolactinemia in systemic lupus erythematosus (SLE) patients with hyperprolactinemia. Polyethylene Glycols 154-157 prolactin Homo sapiens 99-108 11403264-1 2001 The objective of this study was to determine the diagnostic performance of the percentage of serum prolactin (PRL) precipitated with polyethylene glycol (PEG) for the detection of macroprolactinemia in systemic lupus erythematosus (SLE) patients with hyperprolactinemia. Polyethylene Glycols 154-157 prolactin Homo sapiens 110-113 11403264-8 2001 The best cut-off point for percentage of serum PRL precipitated with PEG for detection of macroprolactinemia was > or = 58.4%. Polyethylene Glycols 69-72 prolactin Homo sapiens 47-50 10468974-6 1999 MEASUREMENTS: Prolactin was measured in sera, fractions from gel filtration chromatography and supernatant obtained after PEG precipitation, by the DELFIA fluoroimmunoassay. Polyethylene Glycols 122-125 prolactin Homo sapiens 14-23 10468974-16 1999 CONCLUSIONS: The PEG precipitation test with assessment of "free" prolactin has been shown to be reproducible and sensitive for the detection of macroprolactinaemia. Polyethylene Glycols 17-20 prolactin Homo sapiens 66-75 8180669-2 1994 Diagnosis of possessing the anti-PRL autoantibody was based on the polyethylene glycol method, displacement of the binding of [125I]PRL with the serum by unlabeled PRL and the binding of PRL to protein G, the affinity gel for immunoglobulin G. Prolactin was measured by an immunoradiometric assay that we found was not affected by the anti-PRL autoantibody. Polyethylene Glycols 67-86 prolactin Homo sapiens 33-36