PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 33535487-5 2021 Here, a hybrid lysine-specific histone demethylase inhibitor, MC3324, displaying selective estrogen receptor down-regulator-like activities in BC, was used to highlight the interplay between epigenetic and ERalpha signaling. Lysine 15-21 estrogen receptor 1 Homo sapiens 91-108 33535487-5 2021 Here, a hybrid lysine-specific histone demethylase inhibitor, MC3324, displaying selective estrogen receptor down-regulator-like activities in BC, was used to highlight the interplay between epigenetic and ERalpha signaling. Lysine 15-21 estrogen receptor 1 Homo sapiens 206-213 33330095-0 2020 Lysine in Combination With Estradiol Promote Dissemination of Estrogen Receptor Positive Breast Cancer via Upregulation of U2AF1 and RPN2 Proteins. Lysine 0-6 estrogen receptor 1 Homo sapiens 62-79 30190324-8 2018 In a reconstituted system in bacteria, I analyzed HSP90/P23-associated, SMYD2-mediated ERalpha methylation and found that when SMYD2 binds to the molecular chaperones, it considerably increases methylation of Lys-266 in ERalpha. Lysine 209-212 estrogen receptor 1 Homo sapiens 87-94 32363646-6 2020 We then use our two-step procedure to identify a set of Lysine-related metabolites that potentially mediate the known relationship between increased body mass index and the increased risk of estrogen-receptor positive breast cancer in postmenopausal women. Lysine 56-62 estrogen receptor 1 Homo sapiens 191-208 30190324-8 2018 In a reconstituted system in bacteria, I analyzed HSP90/P23-associated, SMYD2-mediated ERalpha methylation and found that when SMYD2 binds to the molecular chaperones, it considerably increases methylation of Lys-266 in ERalpha. Lysine 209-212 estrogen receptor 1 Homo sapiens 220-227 24125069-5 2013 Here, we report that histone acetyltransferase Hbo1 promotes destabilization of estrogen receptor alpha (ERalpha) in breast cancers through lysine 48-linked ubiquitination. Lysine 140-146 estrogen receptor 1 Homo sapiens 80-103 28174300-5 2017 Exposed glutamate residues in CaM (Glu-11, Glu-14, Glu-84, and Glu-87) form salt bridges with key lysine residues in ER-alpha (Lys-299, Lys-302, and Lys-303), which is likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 127-130 estrogen receptor 1 Homo sapiens 117-125 28174300-5 2017 Exposed glutamate residues in CaM (Glu-11, Glu-14, Glu-84, and Glu-87) form salt bridges with key lysine residues in ER-alpha (Lys-299, Lys-302, and Lys-303), which is likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 136-139 estrogen receptor 1 Homo sapiens 117-125 28174300-5 2017 Exposed glutamate residues in CaM (Glu-11, Glu-14, Glu-84, and Glu-87) form salt bridges with key lysine residues in ER-alpha (Lys-299, Lys-302, and Lys-303), which is likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 136-139 estrogen receptor 1 Homo sapiens 117-125 28167798-6 2017 The ERalpha-binding sites in tamoxifen-associated endometrial tumors differed from those in the tumors from nonusers and had distinct underlying DNA sequences and divergent enhancer activity as marked by histone 3 containing the acetylated lysine 27 (H3K27ac). Lysine 240-246 estrogen receptor 1 Homo sapiens 4-11 25488809-4 2015 We have identified the histone H3 lysine 9 mono- and di-methyl demethylase enzyme KDM3A as a positive regulator of ER activity. Lysine 34-40 estrogen receptor 1 Homo sapiens 115-117 24725156-0 2014 Charge clamps of lysines and hydrogen bonds play key roles in the mechanism to fix helix 12 in the agonist and antagonist positions of estrogen receptor alpha: intramolecular interactions studied by the ab initio fragment molecular orbital method. Lysine 17-24 estrogen receptor 1 Homo sapiens 135-158 28174300-5 2017 Exposed glutamate residues in CaM (Glu-11, Glu-14, Glu-84, and Glu-87) form salt bridges with key lysine residues in ER-alpha (Lys-299, Lys-302, and Lys-303), which is likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 98-104 estrogen receptor 1 Homo sapiens 117-125 28174300-5 2017 Exposed glutamate residues in CaM (Glu-11, Glu-14, Glu-84, and Glu-87) form salt bridges with key lysine residues in ER-alpha (Lys-299, Lys-302, and Lys-303), which is likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 98-104 estrogen receptor 1 Homo sapiens 243-251 26640146-6 2016 The preferential binding of the PHD finger of ASXL2 to the dimethylated H3 lysine 4 may account for its requirement for ERalpha activation. Lysine 75-81 estrogen receptor 1 Homo sapiens 120-127 24594358-4 2014 Here we present the crystal structure of SMYD2 in complex with a target lysine (Lys266)-containing ERalpha peptide. Lysine 72-78 estrogen receptor 1 Homo sapiens 99-106 24594358-5 2014 The structure reveals that ERalpha binds SMYD2 in a U-shaped conformation with the binding specificity determined mainly by residues C-terminal to the target lysine. Lysine 158-164 estrogen receptor 1 Homo sapiens 27-34 24125069-5 2013 Here, we report that histone acetyltransferase Hbo1 promotes destabilization of estrogen receptor alpha (ERalpha) in breast cancers through lysine 48-linked ubiquitination. Lysine 140-146 estrogen receptor 1 Homo sapiens 105-112 22275375-6 2012 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), and Glu(87)) form salt bridges with key lysine residues in ER-alpha (Lys(299), Lys(302), and Lys(303)), which are likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 102-108 estrogen receptor 1 Homo sapiens 121-129 24101509-5 2013 Here we show that SET and MYND domain containing 2 (SMYD2), a histone H3K4 and H3K36 methyltransferase, directly methylates ERalpha protein at lysine 266 (K266) both in vitro and in cells. Lysine 143-149 estrogen receptor 1 Homo sapiens 124-131 22275375-6 2012 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), and Glu(87)) form salt bridges with key lysine residues in ER-alpha (Lys(299), Lys(302), and Lys(303)), which are likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 102-108 estrogen receptor 1 Homo sapiens 251-259 22275375-6 2012 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), and Glu(87)) form salt bridges with key lysine residues in ER-alpha (Lys(299), Lys(302), and Lys(303)), which are likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 131-134 estrogen receptor 1 Homo sapiens 121-129 22275375-6 2012 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), and Glu(87)) form salt bridges with key lysine residues in ER-alpha (Lys(299), Lys(302), and Lys(303)), which are likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 131-134 estrogen receptor 1 Homo sapiens 251-259 22275375-6 2012 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), and Glu(87)) form salt bridges with key lysine residues in ER-alpha (Lys(299), Lys(302), and Lys(303)), which are likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 141-144 estrogen receptor 1 Homo sapiens 121-129 22275375-6 2012 Exposed glutamate residues in CaM (Glu(11), Glu(14), Glu(84), and Glu(87)) form salt bridges with key lysine residues in ER-alpha (Lys(299), Lys(302), and Lys(303)), which are likely to prevent ubiquitination at these sites and inhibit degradation of ER-alpha. Lysine 141-144 estrogen receptor 1 Homo sapiens 121-129 19887647-3 2010 Here we show that ER-alpha proteins with single or double lysine mutations of these motifs (including K303R, a cancer-associated mutant) are resistant to inhibition by BRCA1, even though the mutant ER-alpha proteins retain the ability to bind to BRCA1. Lysine 58-64 estrogen receptor 1 Homo sapiens 18-26 21566084-5 2011 Maximal binding of GATA4 precedes ERalpha binding, and GATA4 is necessary for histone 3 lysine 4 dimethylation at ERalpha binding sites, suggesting that GATA4 is a pioneer factor for ERalpha. Lysine 88-94 estrogen receptor 1 Homo sapiens 114-121 21566084-5 2011 Maximal binding of GATA4 precedes ERalpha binding, and GATA4 is necessary for histone 3 lysine 4 dimethylation at ERalpha binding sites, suggesting that GATA4 is a pioneer factor for ERalpha. Lysine 88-94 estrogen receptor 1 Homo sapiens 114-121 20351197-3 2010 In response to both 17beta-estradiol (E2) and the xenoestrogen diethylstilbestrol, ER signaling via phosphatidylinositol 3-kinase/protein kinase B phosphorylates EZH2 at S21, reducing levels of trimethylation of lysine 27 on histone H3 in hormone-responsive cells. Lysine 212-218 estrogen receptor 1 Homo sapiens 83-85 20351197-4 2010 During windows of uterine development that are susceptible to developmental reprogramming, activation of this ER signaling pathway by diethylstilbestrol resulted in phosphorylation of EZH2 and reduced levels of trimethylation of lysine 27 on histone H3 in chromatin of the developing uterus. Lysine 229-235 estrogen receptor 1 Homo sapiens 110-112 19458056-7 2009 RESULTS: The ERalpha promoter in the ERalpha-negative lines MDA-MB-231, MCF10A, and MCF7-5C show CpG island methylation, histone 3 lysine 9 deacetylation, and decreased chromatin accessibility compared with ERalpha-positive cell lines MCF7 and T47-D. Lysine 131-137 estrogen receptor 1 Homo sapiens 13-20 19955385-9 2009 Blocking ER with ICI 182,780 or mGluR1a with LY 367385 prevented ERalpha trafficking to and from the membrane. Lysine 45-47 estrogen receptor 1 Homo sapiens 65-72 19458056-7 2009 RESULTS: The ERalpha promoter in the ERalpha-negative lines MDA-MB-231, MCF10A, and MCF7-5C show CpG island methylation, histone 3 lysine 9 deacetylation, and decreased chromatin accessibility compared with ERalpha-positive cell lines MCF7 and T47-D. Lysine 131-137 estrogen receptor 1 Homo sapiens 37-44 19458056-7 2009 RESULTS: The ERalpha promoter in the ERalpha-negative lines MDA-MB-231, MCF10A, and MCF7-5C show CpG island methylation, histone 3 lysine 9 deacetylation, and decreased chromatin accessibility compared with ERalpha-positive cell lines MCF7 and T47-D. Lysine 131-137 estrogen receptor 1 Homo sapiens 37-44 18388150-0 2008 Estrogen receptor-alpha hinge-region lysines 302 and 303 regulate receptor degradation by the proteasome. Lysine 37-44 estrogen receptor 1 Homo sapiens 0-23 18388150-2 2008 Dynamic interactions between ERalpha and the protein degradation machinery facilitate the down-regulation process by targeting receptor lysine residues for polyubiquitination. Lysine 136-142 estrogen receptor 1 Homo sapiens 29-36 18388150-4 2008 Two receptor lysines, K302 and K303, located in the hinge-region of ERalpha, serve multiple regulatory functions, and we examined whether these might also regulate receptor polyubiquitination, turnover, and receptor-protein interactions. Lysine 13-20 estrogen receptor 1 Homo sapiens 68-75 18388150-8 2008 Furthermore, ERalpha-AA was resistant to ICI-induced polyubiquitination, suggesting that these lysines are polyubiquitinated in response to the antiestrogen and demonstrate a novel role for these two lysines in the mechanism of action of ICI-induced receptor down-regulation. Lysine 95-102 estrogen receptor 1 Homo sapiens 13-20 18388150-8 2008 Furthermore, ERalpha-AA was resistant to ICI-induced polyubiquitination, suggesting that these lysines are polyubiquitinated in response to the antiestrogen and demonstrate a novel role for these two lysines in the mechanism of action of ICI-induced receptor down-regulation. Lysine 200-207 estrogen receptor 1 Homo sapiens 13-20 17631495-8 2007 Surprisingly, PGC-1beta coactivation of tamoxifen-bound ERalpha depends partially on one of the LXXLL motifs of PGC-1beta and on Lys(362) of the ERalpha LBD (i.e. surfaces implicated in agonist-dependent interactions). Lysine 129-132 estrogen receptor 1 Homo sapiens 56-63 17631495-8 2007 Surprisingly, PGC-1beta coactivation of tamoxifen-bound ERalpha depends partially on one of the LXXLL motifs of PGC-1beta and on Lys(362) of the ERalpha LBD (i.e. surfaces implicated in agonist-dependent interactions). Lysine 129-132 estrogen receptor 1 Homo sapiens 145-152 12970748-4 2003 Stable transfection of parental MCF-7 cells with a dominant-negative Akt mutant, as well as the PI 3-K inhibitors wortmannin and LY 294,002, blocked the effect of estradiol on ER-alpha expression and activity by 70-80 and 55-63%, respectively. Lysine 129-131 estrogen receptor 1 Homo sapiens 176-184 16497729-0 2006 Acetylation of estrogen receptor alpha by p300 at lysines 266 and 268 enhances the deoxyribonucleic acid binding and transactivation activities of the receptor. Lysine 50-57 estrogen receptor 1 Homo sapiens 15-38 16497729-2 2006 Using mutagenesis and mass spectrometry, we identified two conserved lysine residues in ERalpha (Lys266 and Lys268) that are the primary targets of p300-mediated acetylation. Lysine 69-75 estrogen receptor 1 Homo sapiens 88-95 16497729-5 2006 Acetylation at Lys266 and Lys268, or substitution of the same residues with glutamine (i.e. K266/268Q), a residue that mimics acetylated lysine, enhances the DNA binding activity of ERalpha in EMSAs. Lysine 137-143 estrogen receptor 1 Homo sapiens 182-189 15961505-6 2005 Using a series of mutants, we demonstrated that ERalpha is sumoylated at conserved lysine residues within the hinge region. Lysine 83-89 estrogen receptor 1 Homo sapiens 48-55 15313417-7 2004 Point mutations at the lysine residue within the acetylation motif of the AR and ERalpha have been identified in prostate cancer as well as in breast cancer tissue. Lysine 23-29 estrogen receptor 1 Homo sapiens 74-88 16326832-7 2005 Similarly, in the orphan nuclear receptor called estrogen-related receptor 3 (ERR3), the replacement of Asp-273 (the corresponding amino acid to Asp-351 in ERalpha) with lysine abolished constitutive transcriptional activity of ERR3 without affecting DNA-binding activity and impaired the ability of the receptor to interact with p160 coactivators. Lysine 170-176 estrogen receptor 1 Homo sapiens 156-163 15132742-4 2004 RESULTS: Mutations of a highly conserved DBD lysine (ERalpha.K206A/G), lead to super-activation of AP-1 through activation function dependent pathways, up to 200 fold. Lysine 45-51 estrogen receptor 1 Homo sapiens 53-60 15132742-9 2004 This function, which requires the integrity of the conserved lysine, both allows for activation at AP-1 with anti-estrogens (with ERbeta and ERalpha DBD-LBD), and prevents ERalpha from becoming superactive at AP-1 with estrogens. Lysine 61-67 estrogen receptor 1 Homo sapiens 141-148 15132742-9 2004 This function, which requires the integrity of the conserved lysine, both allows for activation at AP-1 with anti-estrogens (with ERbeta and ERalpha DBD-LBD), and prevents ERalpha from becoming superactive at AP-1 with estrogens. Lysine 61-67 estrogen receptor 1 Homo sapiens 172-179 11981030-7 2002 Finally, substitution of lysine residues 302 and 303 of ERalpha for glycine rendered a mutant ERalpha unable to interact with CaM whose transactivation activity became insensitive to W7. Lysine 25-31 estrogen receptor 1 Homo sapiens 56-63 11981030-7 2002 Finally, substitution of lysine residues 302 and 303 of ERalpha for glycine rendered a mutant ERalpha unable to interact with CaM whose transactivation activity became insensitive to W7. Lysine 25-31 estrogen receptor 1 Homo sapiens 94-101 34904415-5 2021 Mechanistically, our ChIP-seq data showed that ERalpha directly binds to the estrogen response element (ERE) site within the ApoA-I gene and establishes an acetylation of histone 3 lysine 27 (H3K27ac)-enriched chromatin microenvironment. Lysine 181-187 estrogen receptor 1 Homo sapiens 47-54 11279135-5 2001 p300, but not P/CAF, selectively and directly acetylated the ERalpha at lysine residues within the ERalpha hinge/ligand binding domain. Lysine 72-78 estrogen receptor 1 Homo sapiens 61-68 11279135-5 2001 p300, but not P/CAF, selectively and directly acetylated the ERalpha at lysine residues within the ERalpha hinge/ligand binding domain. Lysine 72-78 estrogen receptor 1 Homo sapiens 99-106 11279135-7 2001 These ERalpha lysine residues also regulated transcriptional activation by histone deacetylase inhibitors and p300. Lysine 14-20 estrogen receptor 1 Homo sapiens 6-13