PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
12706118-5	2003	We have found up-regulated levels of the cyclin-dependent kinase 2 (cdk2) protein in HDF expressing 143(ala) mutant p53 as compared to senescent controls, together with an increase in p21-free cdk2 which, in conjunction with cyclin E, is able to form an active kinase which can phosphorylate the retinoblastoma protein.	Alanine	104-107	cyclin dependent kinase 2	Homo sapiens	68-72	
14506259-3	2003	The catalytic efficiency of CDK2-cyclin A is impaired 2000-, 10-, and 150-fold, when Pro+1, Lys+2, or Lys+3, respectively, is substituted with Ala in a short synthetic peptide substrate.	Alanine	143-146	cyclin dependent kinase 2	Homo sapiens	28-32	
17804818-5	2007	When a Cdk2 phosphorylation site within Mps1 (T468) is mutated to alanine, Mps1 cannot accumulate at centrosomes or participate in centrosome duplication.	Alanine	66-73	cyclin dependent kinase 2	Homo sapiens	7-11	
12706118-5	2003	We have found up-regulated levels of the cyclin-dependent kinase 2 (cdk2) protein in HDF expressing 143(ala) mutant p53 as compared to senescent controls, together with an increase in p21-free cdk2 which, in conjunction with cyclin E, is able to form an active kinase which can phosphorylate the retinoblastoma protein.	Alanine	104-107	cyclin dependent kinase 2	Homo sapiens	41-66	
11438644-2	2001	Alanine scanning mutagenesis of the Cy motif of the cdk inhibitor p21 revealed that the conserved arginine or leucine (constituting the conserved RXL sequence) was important for p21"s ability to inhibit cyclin E-cdk2 activity.	Alanine	0-7	cyclin dependent kinase 2	Homo sapiens	212-216	
11502743-6	2001	Importantly, rather than inhibiting it, Cyclin A-Cdk2 stimulated the initiation activity of pol-prim containing a triple N-terminal alanine mutant of the p180 subunit.	Alanine	132-139	cyclin dependent kinase 2	Homo sapiens	49-53	
10884347-5	2000	An alanine mutation at any one of four key positions abrogates the efficacy of a synthetic peptide containing this motif as an inhibitor of cyclin A-cdk2 phosphorylation of p53 protein.	Alanine	3-10	cyclin dependent kinase 2	Homo sapiens	149-153	
10995387-12	2000	Importantly, mutation of Cdk2 phosphorylation sites to alanine abrogates the ability of p220 to activate the histone H2B promoter.	Alanine	55-62	cyclin dependent kinase 2	Homo sapiens	25-29	
7784076-2	1995	To investigate the molecular basis of the interaction between these proteins and the cyclin-dependent kinases (CDKs), we performed a systematic mutagenesis of the CKI family member p21Cip1 using the alanine-scanning strategy.	Alanine	199-206	cyclin dependent kinase 2	Homo sapiens	111-115	
10095772-7	1999	Mutation of one of these residues (T524) to alanine diminished the ability of B-Myb to promote transcription of a reporter gene, suggesting that phosphorylation of B-Myb at this site is important for the regulation of its activity by cyclin A/Cdk2.	Alanine	44-51	cyclin dependent kinase 2	Homo sapiens	243-247	
7624134-8	1995	Mutation of serine 315 of p53 to alanine (p53-S315A) abolished phosphorylation by cdk2 kinase.	Alanine	33-40	cyclin dependent kinase 2	Homo sapiens	82-86	
1396589-5	1992	Replacement of T160 with alanine abolishes the kinase activity of CDK2, indicating that phosphorylation at this site (as in CDC2) is required for kinase activity.	Alanine	25-32	cyclin dependent kinase 2	Homo sapiens	66-70	
23853094-5	2013	Consistent with our previous demonstration that CDK2 negatively regulates Treg function, we find that mutation of the serine or threonine at each CDK motif to alanine (S/T A) results in enhanced Foxp3 protein stability in CD4(+) T cells.	Alanine	159-166	cyclin dependent kinase 2	Homo sapiens	48-52