PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 26924013-1 2016 Glycyl-sarcosine (Gly-Sar) is a well-known model substrate for the intestinal uptake of dipeptides through peptide transporter 1 (PepT1). Dipeptides 88-98 solute carrier family 15 member 1 Homo sapiens 107-128 26924013-1 2016 Glycyl-sarcosine (Gly-Sar) is a well-known model substrate for the intestinal uptake of dipeptides through peptide transporter 1 (PepT1). Dipeptides 88-98 solute carrier family 15 member 1 Homo sapiens 130-135 22490229-1 2012 The purpose of this study was to determine whether glycylsarcosine (a model dipeptide) and oseltamivir (an antiviral prodrug) exhibited a species-dependent uptake in yeast Pichia pastoris expressing the rat, mouse, and human homologs of PEPT1. Dipeptides 76-85 solute carrier family 15 member 1 Homo sapiens 237-242 25650381-7 2015 Fluorescence-labeled dipeptide uptake into the cells was observed and was inhibited by ibuprofen, an inhibitor of the intestinal oligopeptide transporter solute carrier 15A1/PEPT1. Dipeptides 21-30 solute carrier family 15 member 1 Homo sapiens 174-179 24903899-0 2014 Simultaneous determination of three dipeptides (JBP485, Gly-Sar and JBP923) in the cell lysates by liquid chromatography-tandem mass spectrometry: application to identify the function of the PEPT1 transfected cell. Dipeptides 36-46 solute carrier family 15 member 1 Homo sapiens 191-196 24422538-1 2014 By taking advantage of the cytotoxic effect of nitric oxide (NO) and PepT1 for molecule-targeted drug delivery, a series of amino acid/dipeptide diester prodrugs of NO-donating oleanolic acid derivatives were designed and synthesized. Dipeptides 135-144 solute carrier family 15 member 1 Homo sapiens 69-74 24082128-3 2013 To obtain insight into this mechanism, we characterize inhibitor binding to the Escherichia coli PTR dipeptide and tripeptide permease A (DtpA), which shows substrate specificities similar to its human homolog hPEPT1. Dipeptides 101-110 solute carrier family 15 member 1 Homo sapiens 210-216 21529830-1 2011 BACKGROUND: To quantify transmembrane transport of dipeptides by PepT1, passive uptake (non-PepT1 mediated) must be subtracted from total (measured) uptake. Dipeptides 51-61 solute carrier family 15 member 1 Homo sapiens 65-70 22263689-0 2012 Synthesis and evaluation of a dipeptide-drug conjugate library as substrates for PEPT1. Dipeptides 30-39 solute carrier family 15 member 1 Homo sapiens 81-86 21529830-1 2011 BACKGROUND: To quantify transmembrane transport of dipeptides by PepT1, passive uptake (non-PepT1 mediated) must be subtracted from total (measured) uptake. Dipeptides 51-61 solute carrier family 15 member 1 Homo sapiens 92-97 21262302-3 2011 The human peptide transporter (PEPT1) is expressed in the intestine and recognizes compounds such as dipeptides and tripeptides. Dipeptides 101-111 solute carrier family 15 member 1 Homo sapiens 31-36 21538757-11 2011 We conclude 1) that free MRPs are not substrates for the intestinal lysine transporter(s), and 2) that dietary MRPs are absorbed into intestinal cells in the form of dipeptides, most likely by the peptide transporter PEPT1. Dipeptides 166-176 solute carrier family 15 member 1 Homo sapiens 217-222 21307350-2 2011 These transport systems are distinct from the previously known H(+)/peptide cotransporters PEPT1 and PEPT2, which transport only dipeptides and tripeptides. Dipeptides 129-139 solute carrier family 15 member 1 Homo sapiens 91-96 21147219-0 2011 Affinity and translocation relationships via hPEPT1 of H-X aa-Ser-OH dipeptides: evaluation of H-Phe-Ser-OH as a pro-moiety for ibuprofen and benzoic acid prodrugs. Dipeptides 69-79 solute carrier family 15 member 1 Homo sapiens 45-51 21147219-2 2011 The aim of the study was to synthesize a series of 11 serine-containing dipeptides (H-X(aa)-Ser-OH) and to investigate the relationship between binding to and transport via hPEPT1. Dipeptides 72-82 solute carrier family 15 member 1 Homo sapiens 173-179 21147219-3 2011 An additional aim was to design a dipeptide which could serve as a pro-moiety for prodrugs targeted to hPEPT1. Dipeptides 34-43 solute carrier family 15 member 1 Homo sapiens 103-109 21147219-6 2011 The K(i)-values of H-X(aa)-Ser-OH dipeptides for hPEPT1 in MDCK/hPEPT1 cells ranged from 0.14 mM (logIC(50)=-0.85 +- 0.06) for H-Tyr-Ser-OH to 0.89 mM (logIC(50)=-0.09 +- 0.02) for H-Gly-Ser-OH, as measured in a competition assay with [(14)C]Gly-Sar. Dipeptides 34-44 solute carrier family 15 member 1 Homo sapiens 49-55 21147219-6 2011 The K(i)-values of H-X(aa)-Ser-OH dipeptides for hPEPT1 in MDCK/hPEPT1 cells ranged from 0.14 mM (logIC(50)=-0.85 +- 0.06) for H-Tyr-Ser-OH to 0.89 mM (logIC(50)=-0.09 +- 0.02) for H-Gly-Ser-OH, as measured in a competition assay with [(14)C]Gly-Sar. Dipeptides 34-44 solute carrier family 15 member 1 Homo sapiens 64-70 21147219-7 2011 The dipeptides were translocated via hPEPT1 with K(m)-values in the range of 0.20 (logIC(50)=-0.69 +- 0.04) for H-Met-Ser-OH to 1.04 (logIC(50)=0.02 +- 0.04) mM for H-Gly-Ser-OH. Dipeptides 4-14 solute carrier family 15 member 1 Homo sapiens 37-43 21147219-8 2011 The relationship between ligand and transportate properties indicated that the initial binding of the ligand to hPEPT1 is the major determinant for translocation of the investigated dipeptides. Dipeptides 182-192 solute carrier family 15 member 1 Homo sapiens 112-118 20307658-2 2010 We here synthesized two novel dipeptides, l-phenylalanyl sarcosine (Phe-Sar) and 4-(4-methoxyphenyl)-l-phenylalanyl sarcosine (Bip(OMe)-Sar), and examined their effect on the growth of human pancreatic cancer AsPC-1 cells, which are known to highly express oligopeptide transporter PEPT1/SLC15A1. Dipeptides 30-40 solute carrier family 15 member 1 Homo sapiens 282-287 20880398-3 2010 The aim of the present study was to investigate SLC36A1-mediated transport of Gly-Gly and Gly-Gly mimetics, and to investigate Gly-Sar transport via SLC36A1 and the proton-coupled dipeptide/tripeptide transporter, SLC15A1 in Caco-2 cells. Dipeptides 180-189 solute carrier family 15 member 1 Homo sapiens 214-221 20307658-2 2010 We here synthesized two novel dipeptides, l-phenylalanyl sarcosine (Phe-Sar) and 4-(4-methoxyphenyl)-l-phenylalanyl sarcosine (Bip(OMe)-Sar), and examined their effect on the growth of human pancreatic cancer AsPC-1 cells, which are known to highly express oligopeptide transporter PEPT1/SLC15A1. Dipeptides 30-40 solute carrier family 15 member 1 Homo sapiens 288-295 19084598-3 2009 This study was conducted to determine the effects of the inflammatory cytokine interferon-gamma (IFN-gamma) on h-PEPT1 mediated dipeptide absorption. Dipeptides 128-137 solute carrier family 15 member 1 Homo sapiens 113-118 20104847-7 2010 In a third approach, uptake of the dipeptides into hPEPT1-transfected HeLa cells was analyzed by HPLC. Dipeptides 35-45 solute carrier family 15 member 1 Homo sapiens 51-57 20104847-9 2010 We conclude that pyrraline containing dipeptides are transported by hPEPT1 in an electrogenic manner into intestinal cells. Dipeptides 38-48 solute carrier family 15 member 1 Homo sapiens 68-74 19685173-1 2009 PURPOSE: The human dipeptide transporter (hPEPT1) facilitates transport of dipeptides and drugs from the intestine into the circulation. Dipeptides 75-85 solute carrier family 15 member 1 Homo sapiens 42-48 18781650-9 2009 These results demonstrate that the addition of a dipeptide moiety to a poorly absorbable nonpeptide/nonamino acid-like drug can result in absorption via the intestinal transporter PEPT1, though there is some selectivity as regards the structure of the added peptide moiety. Dipeptides 49-58 solute carrier family 15 member 1 Homo sapiens 180-185 18652477-1 2008 Dipeptide monoester prodrugs of floxuridine were synthesized, and their chemical stability in buffers, resistance to glycosidic bond metabolism, affinity for PEPT1, enzymatic activation and permeability in cancer cells were determined and compared to those of mono amino acid monoester floxuridine prodrugs. Dipeptides 0-9 solute carrier family 15 member 1 Homo sapiens 158-163 18824524-14 2009 However, they strongly inhibit hPEPT1-mediated uptake of dipeptides and cefadroxil. Dipeptides 57-67 solute carrier family 15 member 1 Homo sapiens 31-37 18485005-10 2008 Taken together, these findings show that yhiP encodes a protein that mediates proton-dependent electrogenic transport of dipeptides and tripeptides with similarities to mammalian PEPT1. Dipeptides 121-131 solute carrier family 15 member 1 Homo sapiens 179-184 17454359-1 2007 The human intestinal dipeptide transporter (hPepT1) transports dipeptides and pharmacologically active drugs from the intestine to the blood. Dipeptides 63-73 solute carrier family 15 member 1 Homo sapiens 44-50 18367661-11 2008 The seven-state ordered kinetic model previously proposed for hPEPT1 accounts for the steady-state kinetics of neutral drug and dipeptide transport by hPEPT2. Dipeptides 128-137 solute carrier family 15 member 1 Homo sapiens 62-68 18389514-0 2008 Dipeptide derivatives of AZT: synthesis, chemical stability, activation in human plasma, hPEPT1 affinity, and antiviral activity. Dipeptides 0-9 solute carrier family 15 member 1 Homo sapiens 89-95 16627568-0 2006 Molecular interactions between dipeptides, drugs and the human intestinal H+ -oligopeptide cotransporter hPEPT1. Dipeptides 31-41 solute carrier family 15 member 1 Homo sapiens 105-111 17487240-3 2006 We have recently demonstrated that the plasma membrane transporter, hPepT1, was able to efficiently translocate muramyl dipeptide (MDP), a specific Nod2-activating molecule, into host cells. Dipeptides 120-129 solute carrier family 15 member 1 Homo sapiens 68-74 16627568-8 2006 Based on steady-state and presteady-state analysis of Gly-Sar and cefadroxil transport, we proposed an extension of the 6-state kinetic model for hPEPT1 function that globally accounts for the observed presteady-state and steady-state kinetics of neutral dipeptide and drug transport. Dipeptides 255-264 solute carrier family 15 member 1 Homo sapiens 146-152 15981923-3 2004 In this study, it was first verified that monkey intestine transports a model dipeptide, Gly-Sar, in a proton-dependent manner (0.30 +/- 0.05 pmol cm(-2) s(-1) at pH 6.0 and 0.10 +/- 0.03 pmol cm(-2) s(-1) at pH 7.4) in the absorptive direction, presumably by monkey PEPT1. Dipeptides 78-87 solute carrier family 15 member 1 Homo sapiens 267-272 16267577-0 2005 Probing dipeptide trans/cis stereochemistry using pH control of thiopeptide analogues, and application to the PepT1 transporter. Dipeptides 8-17 solute carrier family 15 member 1 Homo sapiens 110-115 15934785-0 2005 hPEPT1 affinity and translocation of selected Gln-Sar and Glu-Sar dipeptide derivatives. Dipeptides 66-75 solute carrier family 15 member 1 Homo sapiens 0-6 15934785-4 2005 More information on affinity to and translocation via hPEPT1 of side-chain-modified dipeptides may be gained by conducting a study of selected dipeptide derivatives with variety in size, hydrophobicity, and bond type. Dipeptides 84-94 solute carrier family 15 member 1 Homo sapiens 54-60 15934785-4 2005 More information on affinity to and translocation via hPEPT1 of side-chain-modified dipeptides may be gained by conducting a study of selected dipeptide derivatives with variety in size, hydrophobicity, and bond type. Dipeptides 84-93 solute carrier family 15 member 1 Homo sapiens 54-60 15934785-5 2005 The aim of the present study was to synthesize new esters and amides based on L-Glu-Sar and investigate the effects that bond type and size of modification of the N-terminal side chain of sarcosine-containing dipeptides have on the affinity to and translocation via hPEPT1. Dipeptides 209-219 solute carrier family 15 member 1 Homo sapiens 266-272 16759105-0 2006 Human PEPT1 pharmacophore distinguishes between dipeptide transport and binding. Dipeptides 48-57 solute carrier family 15 member 1 Homo sapiens 6-11 16759105-1 2006 The human intestinal oligopeptide transporter (PEPT1) facilitates the absorption of dipeptides, tripeptides, and many peptidomimetic drugs. Dipeptides 84-94 solute carrier family 15 member 1 Homo sapiens 47-52 16244752-1 2005 The conformation at the first residue of dipeptide substrates for the peptide transporter PepT1 has been probed using constrained peptide analogues, and the active conformation has been identified. Dipeptides 41-50 solute carrier family 15 member 1 Homo sapiens 90-95 16039984-1 2005 Amino acids, a critical energy source for the intestinal epithelial cells, are more efficiently assimilated in the normal intestine via peptide co-transporters such as proton:dipeptide co-transport (such as PepT1). Dipeptides 175-184 solute carrier family 15 member 1 Homo sapiens 207-212 16039984-2 2005 Active uptake of a non-hydrolyzable dipeptide (glycosarcosine) was used as a substrate and PepT1 was found to be present in normal villus, but not crypt cells. Dipeptides 36-45 solute carrier family 15 member 1 Homo sapiens 91-96 16039984-5 2005 Kinetic studies demonstrated that the mechanism of inhibition of PepT1 during chronic enteritis was secondary to a decrease in the affinity of the co-transporter for the dipeptide without an alteration in the maximal rate of uptake (Vmax). Dipeptides 170-179 solute carrier family 15 member 1 Homo sapiens 65-70 15623827-0 2005 Alanylglutamine dipeptide and growth hormone maintain PepT1-mediated transport in oxidatively stressed Caco-2 cells. Dipeptides 16-25 solute carrier family 15 member 1 Homo sapiens 54-59 15623827-4 2005 Uptake of [(14)C]glycylsarcosine (Gly-Sar) was used to evaluate PepT1-mediated dipeptide transport. Dipeptides 79-88 solute carrier family 15 member 1 Homo sapiens 64-69 14669333-1 2003 AIM: To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald rats. Dipeptides 87-96 solute carrier family 15 member 1 Homo sapiens 108-113 14706812-0 2004 Analysis of the transport properties of side chain modified dipeptides at the mammalian peptide transporter PEPT1. Dipeptides 60-70 solute carrier family 15 member 1 Homo sapiens 108-113 14706812-1 2004 This study was initiated to examine systematically the effect of side chain modifications at dipeptides on their transport via PEPT1. Dipeptides 93-103 solute carrier family 15 member 1 Homo sapiens 127-132 14706812-6 2004 Substitution of a terminal phenyl residue in the side chain blocking group by a p-nitrophenyl residue enhanced the affinity of several dipeptide derivatives for interaction with PEPT1. Dipeptides 135-144 solute carrier family 15 member 1 Homo sapiens 178-183 14520683-1 2003 Ceftibuten uptake into Caco-2 cells and intestinal brush border membrane vesicles is mediated by the dipeptide transport system (PEPT1). Dipeptides 101-110 solute carrier family 15 member 1 Homo sapiens 129-134 14561585-1 2003 The abundance of the oligopeptide transporter (Pept-1) in the brush-border membrane of the intestinal epithelium is the central mechanism for regulation of transport of products of protein digestion (dipeptides and tripeptides) and peptidomimetic drugs (for example, beta-lactam antibiotics). Dipeptides 200-210 solute carrier family 15 member 1 Homo sapiens 47-53 14561585-5 2003 In the case of dipeptides, epidermal growth factor, and thyroid hormone, there are parallel changes in the gene expression brought about by alteration of transcription and/or stability of Pept-1 mRNA. Dipeptides 15-25 solute carrier family 15 member 1 Homo sapiens 188-194 12780388-2 2003 In the present study we evaluated the effects of epidermal growth factor (EGF) and insulin on hPepT1-mediated dipeptide uptake in the intestinal cell line Caco-2. Dipeptides 110-119 solute carrier family 15 member 1 Homo sapiens 94-100 12587121-3 2003 Inhibition of Gly-Sar uptake into Caco-2 cells was measured in the presence of dipeptides and xenobiotics exhibiting various binding affinities for the PepT-1. Dipeptides 79-89 solute carrier family 15 member 1 Homo sapiens 152-158 12598410-2 2003 Optimal dipeptide and peptidomimetic drug transport across the intestinal mucosal surface is dependent upon the co-operative functional activity of the di/tripeptide transporter hPepT1 and the Na(+)/H(+) exchanger NHE3. Dipeptides 8-17 solute carrier family 15 member 1 Homo sapiens 178-184 12598410-17 2003 These observations demonstrate that VIP (and PACAP) modulate activity of the H(+)/dipeptide transporter hPepT1 in a Na(+)-dependent manner consistent with the modulation being indirect through inhibition of NHE3. Dipeptides 82-91 solute carrier family 15 member 1 Homo sapiens 104-110 12397398-4 2002 The physiological problem addressed here was to determine how two transporters (hPepT1 and NHE3), involved in nutrient absorption and pH(i) homeostasis, function co-operatively to maximise dipeptide absorption when both operate sub-optimally at typical mucosal surface pH values (pH 6.1-6.8). Dipeptides 189-198 solute carrier family 15 member 1 Homo sapiens 80-86 12538834-10 2003 Overall, the results suggest that the dipeptide prodrugs of ACV have a high affinity toward the intestinal oligopeptide transporter hPEPT1 and therefore seem to be promising candidates in the treatment of ocular and oral herpesvirus infections, because cornea and intestinal epithelia seem to express the oligopeptide transporters. Dipeptides 38-47 solute carrier family 15 member 1 Homo sapiens 132-138 12526824-1 2003 A general drug delivery approach for increasing oral bioavailability of purine and pyrimidine analogues such as acyclovir may be to link these compounds reversibly to stabilized dipeptide pro-moieties with affinity for the human intestinal di/tri-peptide transporter, hPepT1. Dipeptides 178-187 solute carrier family 15 member 1 Homo sapiens 268-274 12415572-6 2002 Glycyl proline, a dipeptide transport system (PEPT1) substrate, inhibited ceftibuten uptake into Caco-2 cells. Dipeptides 18-27 solute carrier family 15 member 1 Homo sapiens 46-51 12082113-6 2002 However, binding and transport of cationic dipeptides shows a pronounced selectivity for the position of charged side chains demonstrating that the binding domain of PEPT1 is asymmetric, both in its inward and outward facing conformation. Dipeptides 43-53 solute carrier family 15 member 1 Homo sapiens 166-171 12356285-5 2002 Michaelis constants (Km) for these cyclic dipeptides were cyclo(Ser-Tyr) < cyclo(Phe-Ser), and cyclo(Gly-Tyr) < cyclo(Gly-Phe), indicating that tyrosine possessing phenol moiety has higher affinity for PEPT1 than phenylalanine possessing benzen moiety. Dipeptides 42-52 solute carrier family 15 member 1 Homo sapiens 208-213 12403071-1 2002 PURPOSE: The aim of the present study was to evaluate whether the transepithelial transport of the anticancer compound 4-toluenesulfonylureido-carnosine (Ts-carnosine) and the dipeptide moiety L-carnosine was due to a hPepT1 carrier-mediated flux. Dipeptides 176-185 solute carrier family 15 member 1 Homo sapiens 218-224 12397398-5 2002 Functional hPepT1 activity in human intestinal epithelial (Caco-2) cell monolayers was determined by measurement of apical uptake and apical-to-basolateral transport of the dipeptide glycylsarcosine. Dipeptides 173-182 solute carrier family 15 member 1 Homo sapiens 11-17 11714740-0 2001 PepT1-mediated epithelial transport of dipeptides and cephalexin is enhanced by luminal leptin in the small intestine. Dipeptides 39-49 solute carrier family 15 member 1 Homo sapiens 0-5 12645993-8 2002 Decreases in pHi were observed in CHO cells expressing the human H+/peptide transporter PEPT1 upon addition of dipeptide substrates. Dipeptides 111-120 solute carrier family 15 member 1 Homo sapiens 88-93 11532319-3 2001 Model ester prodrugs using the stabilized dipeptides D-Glu-Ala and D-Asp-Ala as pro-moieties for benzyl alcohol have been shown to maintain affinity for hPepT1. Dipeptides 42-52 solute carrier family 15 member 1 Homo sapiens 153-159 11557350-9 2001 This suggests that only compounds with a certain intrinsic aqueous solubility should be targeted to hPepT1 by attachment to a dipeptide. Dipeptides 126-135 solute carrier family 15 member 1 Homo sapiens 100-106 11457645-2 2001 The human peptide transporter, hPepT1, situated in the small intestine, may be exploited to increase absorption of drugs or model drugs by attaching them to a dipeptide, which is recognised by hPepT1. Dipeptides 159-168 solute carrier family 15 member 1 Homo sapiens 31-37 11518682-8 2001 This is the first study that visualizes dipeptide transport across the mammalian intestine and indicates that uptake assays using D-Ala-Lys-AMCA might be useful for characterizing PEPT1-specific substrates or inhibitors. Dipeptides 40-49 solute carrier family 15 member 1 Homo sapiens 180-185 11457645-2 2001 The human peptide transporter, hPepT1, situated in the small intestine, may be exploited to increase absorption of drugs or model drugs by attaching them to a dipeptide, which is recognised by hPepT1. Dipeptides 159-168 solute carrier family 15 member 1 Homo sapiens 193-199 17024033-5 2000 An oligopeptide transporter, PepT1, is transcriptionally upregulated by certain dietary amino acids and dipeptides. Dipeptides 104-114 solute carrier family 15 member 1 Homo sapiens 29-34 11337056-2 2001 Model ester prodrugs using the stabilized dipeptides D-Glu-Ala and D-Asp-Ala as pro-moieties for benzyl alcohol have been shown to have affinity for hPepT1. Dipeptides 42-52 solute carrier family 15 member 1 Homo sapiens 149-155 11284702-2 2001 We provide evidence that the dipeptide derivative Lys[Z(NO(2))]-Pro is an effective competitive inhibitor of mammalian PEPT1 with an apparent binding affinity of 5-10 microM. Dipeptides 29-38 solute carrier family 15 member 1 Homo sapiens 119-124 11284702-6 2001 The fact that Lys[Z(NO(2))]-Pro can bind to PEPT1 from the extracellular as well as the intracellular site was shown in the oocyte expression system by a strong inhibition of dipeptide-induced currents under voltage clamp conditions. Dipeptides 175-184 solute carrier family 15 member 1 Homo sapiens 44-49 11169462-3 2001 We used human intestinal Caco-2 cells to investigate whether the absorption of dipeptides by the peptide transporter PEPT1 alters the apical uptake of free cationic and neutral amino acids. Dipeptides 79-89 solute carrier family 15 member 1 Homo sapiens 117-122 10913752-8 2000 All the stabilized dipeptides may have potential as drug carriers targeting hPepT1. Dipeptides 19-29 solute carrier family 15 member 1 Homo sapiens 76-82 17024033-6 2000 Surprisingly, both insulin and fasting double the maximum velocity for dipeptide uptake (via PepT1), but they act by different mechanisms. Dipeptides 71-80 solute carrier family 15 member 1 Homo sapiens 93-98 11483006-3 2001 Yeast cells transformed with tagged hPEPT1 exhibited 30-fold increased dipeptide uptake compared to control cells with a substrate specificity and pH dependence similar to the native transporter. Dipeptides 71-80 solute carrier family 15 member 1 Homo sapiens 36-42 11169462-0 2001 PEPT1-mediated uptake of dipeptides enhances the intestinal absorption of amino acids via transport system b(0,+). Dipeptides 25-35 solute carrier family 15 member 1 Homo sapiens 0-5 10561591-1 1999 To elucidate the decisive structural factors relevant for dipeptide-carrier interaction, the affinity of short amide and imide derivatives for the intestinal H+/peptide symporter (PEPT1) was investigated by measuring their ability to inhibit Gly-Sar transport in Caco-2 cells. Dipeptides 58-67 solute carrier family 15 member 1 Homo sapiens 180-185 10561591-12 1999 However, in contrast to current knowledge, several Pro-Xaa dipeptides such as Pro-Leu, Pro-Tyr and Pro-Pro are recognized by PEPT1 with appreciable affinities. Dipeptides 59-69 solute carrier family 15 member 1 Homo sapiens 125-130 10213369-0 1999 Evidence for overlapping substrate specificity between large neutral amino acid (LNAA) and dipeptide (hPEPT1) transporters for PD 158473, an NMDA antagonist. Dipeptides 91-100 solute carrier family 15 member 1 Homo sapiens 102-108 9893960-0 1999 Conjugation of dipeptide to fluorescent dyes enhances its affinity for a dipeptide transporter (PEPT1) in human intestinal Caco-2 cells. Dipeptides 15-24 solute carrier family 15 member 1 Homo sapiens 96-101 9508831-8 1998 This was in part due to an increase in hPepT1 mRNA half-life from 8.9 +/- 1.1 to 12.5 +/- 1.6 h (n = 3), but the increase in half-life does not account fully for the observed increase in mRNA levels, suggesting that there was also a dipeptide-mediated increase in hPepT1 transcription. Dipeptides 233-242 solute carrier family 15 member 1 Homo sapiens 39-45 9508831-7 1998 The dipeptide-induced increase in substrate transport was accompanied by a parallel increase of 1.92 (+/- 0.30)-fold (n = 9) in hPepT1 mRNA. Dipeptides 4-13 solute carrier family 15 member 1 Homo sapiens 128-134 9307302-11 1997 This is the first report showing that a dipeptide transport system, which is similar but not identical to the well-characterized oligopeptide transporters PepT1 and PepT2, exists in fibroblast-derived tumor cells but not in normal fibroblasts. Dipeptides 40-49 solute carrier family 15 member 1 Homo sapiens 155-160 9299407-6 1997 When expressed in Xenopus oocytes, hPEPT1-RF showed no transport activity of glycylsarcosine, but shifted pH profile of the dipeptide transport mediated by the coexpressed hPEPT1. Dipeptides 124-133 solute carrier family 15 member 1 Homo sapiens 35-41 9299407-6 1997 When expressed in Xenopus oocytes, hPEPT1-RF showed no transport activity of glycylsarcosine, but shifted pH profile of the dipeptide transport mediated by the coexpressed hPEPT1. Dipeptides 124-133 solute carrier family 15 member 1 Homo sapiens 172-178 9207295-7 1997 These studies suggest that the insulin stimulates dipeptide transport by increasing membrane insertion of oligopeptide transporter from a preformed cytoplasmic pool, and cholera toxin decreases dipeptide transport by inhibiting the activity of Pept-1 through an increase in the intracellular concentration of adenosine 3",5"-cyclic monophosphate. Dipeptides 50-59 solute carrier family 15 member 1 Homo sapiens 244-250 9207295-7 1997 These studies suggest that the insulin stimulates dipeptide transport by increasing membrane insertion of oligopeptide transporter from a preformed cytoplasmic pool, and cholera toxin decreases dipeptide transport by inhibiting the activity of Pept-1 through an increase in the intracellular concentration of adenosine 3",5"-cyclic monophosphate. Dipeptides 194-203 solute carrier family 15 member 1 Homo sapiens 244-250 9950279-3 1999 RESULTS: Ceftibuten and cyclacillin were recognized by PEPT1 with affinity constants comparable to those of natural dipeptides (K(i) = 0.3 and 0.5 mM, respectively). Dipeptides 116-126 solute carrier family 15 member 1 Homo sapiens 55-60 9790685-1 1998 We have used epitope insertion to analyze the transmembrane topology of the human H+-dipeptide symporter hPEPT1. Dipeptides 85-94 solute carrier family 15 member 1 Homo sapiens 105-111 9790685-5 1998 EE106-, 412-, and 708-hPEPT1 transported the dipeptide tracer as well as wild-type hPEPT1. Dipeptides 45-54 solute carrier family 15 member 1 Homo sapiens 22-28 9790685-9 1998 Epitope insertions at regions linking the putative TMD1 and TMD2, and TMD2 and TMD3 (EE39- and EE78-hPEPT1), abolished the dipeptide transport into the cells. Dipeptides 123-132 solute carrier family 15 member 1 Homo sapiens 100-106 9755888-13 1998 CONCLUSIONS: Adenoviral infected Hela cells displayed a pronounced level of hPepT1 expression with a low background and high specificity to dipeptides. Dipeptides 140-150 solute carrier family 15 member 1 Homo sapiens 76-82 9686684-1 1998 The initial objective of this study was to investigate whether the presence of dipeptide in the culture medium stimulates the uptake of dipeptide by a human intestinal cell line that expresses the oligopeptide transporter (Pept-1). Dipeptides 79-88 solute carrier family 15 member 1 Homo sapiens 223-229 9686684-1 1998 The initial objective of this study was to investigate whether the presence of dipeptide in the culture medium stimulates the uptake of dipeptide by a human intestinal cell line that expresses the oligopeptide transporter (Pept-1). Dipeptides 136-145 solute carrier family 15 member 1 Homo sapiens 223-229 9686684-6 1998 In conclusion, the results show that dipeptides stimulate their own transport by increasing the membrane population of Pept-1. Dipeptides 37-47 solute carrier family 15 member 1 Homo sapiens 119-125 9893960-4 1999 Kinetic analysis and effects of addition either of uncoupler (protonophore) or by Gly-Sar, one of the good substrates of PEPT1, revealed that fluorescent dipeptides were taken up by passive diffusion. Dipeptides 154-164 solute carrier family 15 member 1 Homo sapiens 121-126 8914574-0 1996 The human intestinal H+/oligopeptide cotransporter hPEPT1 transports differently-charged dipeptides with identical electrogenic properties. Dipeptides 89-99 solute carrier family 15 member 1 Homo sapiens 51-57 9092716-3 1997 The uptake of the dipeptide Gly-Sar mediated by PEPT 1 or PEPT 2 in these cells was inhibited significantly by the anionic cephalosporins, with the following order of potency: ceftibuten > cefixime > cefdinir. Dipeptides 18-27 solute carrier family 15 member 1 Homo sapiens 48-54 9126331-3 1997 Functional expression of PepT1 was determined in different recombinant clones by flux studies employing the radiolabeled dipeptide 3H-(D)-Phe-(L)-Ala. One clone (GS-PepT1) displayed high level functional expression that was pH dependent and saturable with an app. Dipeptides 121-130 solute carrier family 15 member 1 Homo sapiens 25-30 9126331-5 1997 Inhibition of 3H-(D)-Phe-(L)-Ala uptake into GS-PepT1 by selected dipeptides, tripeptides and peptidomimetics including beta-lactam antibiotics and ACE-inhibitors revealed the same substrate specifity as reported for PepT1 when expressed in mammalian cells or Xenopus laevis oocytes. Dipeptides 66-76 solute carrier family 15 member 1 Homo sapiens 48-53 8914574-1 1996 The human intestinal H+/oligopeptide cotransporter hPEPT1, expressed in Xenopus oocytes, transported neutral, anionic and cationic dipeptides with identical electrogenic properties and maximal evoked currents. Dipeptides 131-141 solute carrier family 15 member 1 Homo sapiens 51-57 8789117-6 1996 Studies of the mammalian oligopeptide transporter PepT1 and the Na(+)- and K(+)-coupled epithelial and neuronal glutamate transporter EAAC1 expressed in oocytes demonstrate that transport of the dipeptide Trp-Gly via PepT1 and transport of Na+ and K+ via EAAC1 across the oocyte plasma membrane can be monitored by measuring intracellular tryptophan absorption and by indirect UV detection of inorganic ions, respectively. Dipeptides 195-204 solute carrier family 15 member 1 Homo sapiens 50-55 7495840-6 1995 The human intestinal H+/peptide cotransporter PEPT 1 has 4-fold less affinity for the dipeptide under identical experimental conditions. Dipeptides 86-95 solute carrier family 15 member 1 Homo sapiens 46-52 8789117-6 1996 Studies of the mammalian oligopeptide transporter PepT1 and the Na(+)- and K(+)-coupled epithelial and neuronal glutamate transporter EAAC1 expressed in oocytes demonstrate that transport of the dipeptide Trp-Gly via PepT1 and transport of Na+ and K+ via EAAC1 across the oocyte plasma membrane can be monitored by measuring intracellular tryptophan absorption and by indirect UV detection of inorganic ions, respectively. Dipeptides 195-204 solute carrier family 15 member 1 Homo sapiens 217-222 35546503-2 2022 Many studies have confirmed that PEPT1 plays a critical role in the absorption of dipeptides, tripeptides, and pseudopeptides in the intestinal tract. Dipeptides 82-92 solute carrier family 15 member 1 Homo sapiens 33-38 35634396-7 2022 Moreover, the Lys-Lys dipeptide downregulated the expression of jejunal Slc7a1, Slc7a2, and Slc15a1 and ileal Slc7a2. Dipeptides 22-31 solute carrier family 15 member 1 Homo sapiens 92-99 35481706-10 2022 The inclusion of glycyl-sarcosine with lysyl-lysine abolished the dipeptide effects on whole body and tissue-specific protein synthesis (P<0.05), suggesting that improved lysine availability was mediated by PepT1. Dipeptides 66-75 solute carrier family 15 member 1 Homo sapiens 207-212 7592745-10 1995 Again, the PEPT 1 cDNA-induced dipeptide uptake was inhibited more potently by cyclacillin than by cefadroxil, and the PEPT 2 cDNA-induced dipeptide uptake was inhibited more potently by cefadroxil than by cyclacillin. Dipeptides 31-40 solute carrier family 15 member 1 Homo sapiens 11-17 7592745-10 1995 Again, the PEPT 1 cDNA-induced dipeptide uptake was inhibited more potently by cyclacillin than by cefadroxil, and the PEPT 2 cDNA-induced dipeptide uptake was inhibited more potently by cefadroxil than by cyclacillin. Dipeptides 139-148 solute carrier family 15 member 1 Homo sapiens 11-17 34371711-1 2021 The peptide transporter PEPT-1 (SLC15A1) plays a major role in nutritional supply with amino acids by mediating the intestinal influx of dipeptides and tripeptides generated during food digestion. Dipeptides 137-147 solute carrier family 15 member 1 Homo sapiens 24-30 34371711-3 2021 The dipeptide glycyl-sarcosine (Gly-Sar), which comprises an N-methylated peptide bond that increases stability against enzymatic degradation, is widely utilized for studying PEPT-1-mediated transport. Dipeptides 4-13 solute carrier family 15 member 1 Homo sapiens 175-181 30302566-11 2019 Conversely, the two dipeptides seems to have similar interaction with the PEPT1 transporter. Dipeptides 20-30 solute carrier family 15 member 1 Homo sapiens 74-79 30833090-3 2019 Here, by using dipeptides of BPA and tyrosine (BPA-Tyr and Tyr-BPA), we propose a novel strategy of selective boron delivery into tumor cells via oligopeptide transporter PEPT1 upregulated in various cancers. Dipeptides 15-25 solute carrier family 15 member 1 Homo sapiens 171-176 30833090-9 2019 The BPA-containing dipeptides would have a potential for the development of novel boron carriers targeting PEPT1. Dipeptides 19-29 solute carrier family 15 member 1 Homo sapiens 107-112 30644743-3 2019 In contrast, a homologue from Escherichia coli, DtpA (dipeptide and tripeptide permease), shows a high similarity to human PepT1 (SLC15A1) in terms of ligand selectivity and transports a similar set of drugs. Dipeptides 54-63 solute carrier family 15 member 1 Homo sapiens 123-128 30644743-3 2019 In contrast, a homologue from Escherichia coli, DtpA (dipeptide and tripeptide permease), shows a high similarity to human PepT1 (SLC15A1) in terms of ligand selectivity and transports a similar set of drugs. Dipeptides 54-63 solute carrier family 15 member 1 Homo sapiens 130-137 29890854-0 2018 Dipeptide-modified nanoparticles to facilitate oral docetaxel delivery: new insights into PepT1-mediated targeting strategy. Dipeptides 0-9 solute carrier family 15 member 1 Homo sapiens 90-95 29890854-3 2018 Herein, we modify Poly (lactic-co-glycolic acid) (PLGA) NPs with the conjugates of dipeptides (L-valine-valine, L-valine-phenylalanine) and polyoxyethylene (PEG Mw: 1000, 2000) stearate to facilitate oral delivery of docetaxel (DTX) to investigate the oral absorption mechanism and regulatory effects on PepT1 of the dipeptide-modified NPs. Dipeptides 83-93 solute carrier family 15 member 1 Homo sapiens 304-309 29890854-3 2018 Herein, we modify Poly (lactic-co-glycolic acid) (PLGA) NPs with the conjugates of dipeptides (L-valine-valine, L-valine-phenylalanine) and polyoxyethylene (PEG Mw: 1000, 2000) stearate to facilitate oral delivery of docetaxel (DTX) to investigate the oral absorption mechanism and regulatory effects on PepT1 of the dipeptide-modified NPs. Dipeptides 83-92 solute carrier family 15 member 1 Homo sapiens 304-309 29890854-4 2018 The cellular uptake of the dipeptide-modified NPs is more efficient than that of the unmodified NPs in the stably transfected hPepT1- Hela cells and Caco-2 cells, suggesting the involvement of PepT1 in the endocytosis of NPs. Dipeptides 27-36 solute carrier family 15 member 1 Homo sapiens 126-132 29890854-4 2018 The cellular uptake of the dipeptide-modified NPs is more efficient than that of the unmodified NPs in the stably transfected hPepT1- Hela cells and Caco-2 cells, suggesting the involvement of PepT1 in the endocytosis of NPs. Dipeptides 27-36 solute carrier family 15 member 1 Homo sapiens 127-132 28012236-5 2017 Meanwhile, Lys deprivation upregulated pept1 expression, which might improve Lys-Lys dipeptide absorption to compensate for the reduced Lys availability. Dipeptides 85-94 solute carrier family 15 member 1 Homo sapiens 39-44