PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 35063765-4 2022 Depletion of biotin from culture medium markedly reduced biotinylation of the two most abundant biotin-carboxylases, acetyl-CoA carboxylase and pyruvate carboxylase. Biotin 13-19 pyruvate carboxylase Homo sapiens 144-164 2679223-2 1989 The natural form of pyruvate carboxylase contains four covalently bound biotins (one per subunit) and it can be considered as an oligosubstituted enzyme-biotin conjugate. Biotin 72-79 pyruvate carboxylase Homo sapiens 20-40 2679223-2 1989 The natural form of pyruvate carboxylase contains four covalently bound biotins (one per subunit) and it can be considered as an oligosubstituted enzyme-biotin conjugate. Biotin 72-78 pyruvate carboxylase Homo sapiens 20-40 3101494-8 1987 Thus, whereas the North American presentation of PC deficiency is associated with the presence of a mature biotin containing protein of the correct molecular weight, the French presentation may, in some (but not in all) cases, have both absent PC protein and absent PC mRNA. Biotin 107-113 pyruvate carboxylase Homo sapiens 49-51 3555348-0 1987 Sequence homology around the biotin-binding site of human propionyl-CoA carboxylase and pyruvate carboxylase. Biotin 29-35 pyruvate carboxylase Homo sapiens 88-108 3814578-4 1986 The pH profiles of the full reverse reaction of pyruvate carboxylase in which oxalacetate decarboxylation is coupled to ATP formation and where Pi is the variable substrate do, however, indicate that such an acid-base catalyst is involved in the other partial reaction of the enzyme in proton transfer to and from biotin. Biotin 314-320 pyruvate carboxylase Homo sapiens 48-68 3918814-1 1985 We have developed a method for rapid differential diagnosis of isolated or multiple deficiencies of the 3 mitochondrial biotin-dependent carboxylases: propionyl-CoA (PCC), 3-methylcrotonyl-CoA (MCC) and pyruvate carboxylase (PC), and for simultaneous evaluation of biotin-responsiveness using a single blood sample. Biotin 120-126 pyruvate carboxylase Homo sapiens 203-223 2868710-1 1985 Incubation of cultured cells with [3H]biotin leads to the labelling of acetyl-CoA carboxylase, pyruvate carboxylase, propionyl-CoA carboxylase and methylcrotonyl-CoA carboxylase. Biotin 38-44 pyruvate carboxylase Homo sapiens 95-115 3918814-1 1985 We have developed a method for rapid differential diagnosis of isolated or multiple deficiencies of the 3 mitochondrial biotin-dependent carboxylases: propionyl-CoA (PCC), 3-methylcrotonyl-CoA (MCC) and pyruvate carboxylase (PC), and for simultaneous evaluation of biotin-responsiveness using a single blood sample. Biotin 265-271 pyruvate carboxylase Homo sapiens 203-223 6548474-2 1984 An oligonucleotide probe specific for the amino acid sequence at the biotin site in pyruvate carboxylase was used to screen a human liver cDNA library. Biotin 69-75 pyruvate carboxylase Homo sapiens 84-104 3939532-3 1985 The six isolated pyruvate carboxylase mutants (two cross-reacting material CRM -ve and four CRM +ve) failed to complement each other, but did complement a form of biotin responsive MCD. Biotin 163-169 pyruvate carboxylase Homo sapiens 17-37 6548474-4 1984 The biotin site amino acid sequence of human pyruvate carboxylase agreed perfectly with that of the sheep enzyme in 14 consecutive positions. Biotin 4-10 pyruvate carboxylase Homo sapiens 45-65 6548474-5 1984 The highly conserved amino acid sequence, Ala-Met-Lys-Met, found at the biotin site in most biotin-containing carboxylases was also present in human pyruvate carboxylase. Biotin 72-78 pyruvate carboxylase Homo sapiens 149-169 6548474-5 1984 The highly conserved amino acid sequence, Ala-Met-Lys-Met, found at the biotin site in most biotin-containing carboxylases was also present in human pyruvate carboxylase. Biotin 92-98 pyruvate carboxylase Homo sapiens 149-169 7104292-0 1982 The determination of the availability to chicks of biotin in feed ingredients by a bioassay based on the response of blood pyruvate carboxylase (EC 6.4.1.1) activity. Biotin 51-57 pyruvate carboxylase Homo sapiens 123-143 6851181-0 1983 The measurement of propionyl-CoA carboxylase and pyruvate carboxylase activity in hair roots: its use in the diagnosis of inherited biotin-dependent enzyme deficiencies. Biotin 132-138 pyruvate carboxylase Homo sapiens 49-69 6851181-1 1983 Two mitochondrial biotin-dependent enzymes, propionyl-CoA carboxylase and pyruvate carboxylase, are measurable in hair roots. Biotin 18-24 pyruvate carboxylase Homo sapiens 74-94 7104292-2 1982 Experiments were carried out to establish the conditions required for the measurement of the availability to chicks of biotin in feed ingredients by a bioassay based on the response of blood pyruvate carboxylase (EC 6.4.1; PC) activity. Biotin 119-125 pyruvate carboxylase Homo sapiens 191-211 7340821-1 1981 The active site of pyruvate carboxylase, like those of all biotin-dependent carboxylases, is believed to consist of two spatially distinct sub-sites with biotin acting as a mobile carboxy-group carrier oscillating between the two sub-sites. Biotin 59-65 pyruvate carboxylase Homo sapiens 19-39 6803393-5 1982 Deficient pyruvate carboxylase activity less than 6 pmoles/min per mg protein was demonstrated in the lymphoblasts from a patient with biotin-dependent multiple carboxylase deficiency. Biotin 135-141 pyruvate carboxylase Homo sapiens 10-30 7340821-1 1981 The active site of pyruvate carboxylase, like those of all biotin-dependent carboxylases, is believed to consist of two spatially distinct sub-sites with biotin acting as a mobile carboxy-group carrier oscillating between the two sub-sites. Biotin 154-160 pyruvate carboxylase Homo sapiens 19-39 6112081-1 1981 Multiple carboxylase deficiency has previously been characterized by deficient activity of three biotin-dependent enzymes: propionyl CoA carboxylase, pyruvate carboxylase and beta-methylcrotonyl CoA carboxylase. Biotin 97-103 pyruvate carboxylase Homo sapiens 150-170 6794361-1 1981 Three biotin-dependent enzymes, pyruvate carboxylase (PC), propionyl CoA carboxylase (PCC), and beta-methylcrotonyl CoA carboxylase (beta MCC), were biochemically characterized in fibroblasts from two patients with neonatal multiple carboxylase deficiency. Biotin 6-12 pyruvate carboxylase Homo sapiens 32-52 7317813-4 1981 Biotin deficiency decreased hepatic activity of pyruvate carboxylase [EC 6.4.1.1] but activity of acetyl-CoA carboxylase [EC 6.4.1.2] was comparatively unaffected. Biotin 0-6 pyruvate carboxylase Homo sapiens 48-68 115903-2 1979 Fibroblast cultures from two individuals with biotin-responsive organicacidemia were found to have a pleiotropic deficiency of propionyl-CoA carboxylase, beta-methylcrotonyl-CoA carboxylase, and pyruvate carboxylase activities after growth in biotin limited culture medium, conditions which do not affect the carboxylase activities of normal cells. Biotin 46-52 pyruvate carboxylase Homo sapiens 195-215 6776949-2 1980 Complementation, manifested as a three- to sevenfold increase in pyruvate carboxylase activity, was observed in fusions between a biotin-responsive multiple carboxylase (pyruvate carboxylase, propionyl CoA carboxylase, and beta-methylcrotonyl CoA carboxylase) deficient fibroblast line and two other lines deficient only in pyruvate carboxylase activity. Biotin 130-136 pyruvate carboxylase Homo sapiens 65-85 6776949-2 1980 Complementation, manifested as a three- to sevenfold increase in pyruvate carboxylase activity, was observed in fusions between a biotin-responsive multiple carboxylase (pyruvate carboxylase, propionyl CoA carboxylase, and beta-methylcrotonyl CoA carboxylase) deficient fibroblast line and two other lines deficient only in pyruvate carboxylase activity. Biotin 130-136 pyruvate carboxylase Homo sapiens 170-190 6776949-2 1980 Complementation, manifested as a three- to sevenfold increase in pyruvate carboxylase activity, was observed in fusions between a biotin-responsive multiple carboxylase (pyruvate carboxylase, propionyl CoA carboxylase, and beta-methylcrotonyl CoA carboxylase) deficient fibroblast line and two other lines deficient only in pyruvate carboxylase activity. Biotin 130-136 pyruvate carboxylase Homo sapiens 170-190 8990-9 1976 The PC preparation was shown to have an absolute requirement for CoASAc, contained biotin, was not inhibited by lipoate, and had an apparent Km approximately equal to 3.6 X 10(-4) M for pyruvate. Biotin 83-89 pyruvate carboxylase Homo sapiens 4-6 555586-0 1979 Pyruvate carboxylase activities in red blood cells and liver of chicks and their dependency on biotin status. Biotin 95-101 pyruvate carboxylase Homo sapiens 0-20 555586-1 1979 First results with activation assays.. Pyruvate carboxylase activities of erythrocytes and liver preparations and their in vitro stimulation by biotin were used for the determination of the biotin status of chicks. Biotin 190-196 pyruvate carboxylase Homo sapiens 39-59 555586-4 1979 The pyruvate carboxylase activation assay appears to be a useful tool for assessing the biotin status of chicks. Biotin 88-94 pyruvate carboxylase Homo sapiens 4-24 562132-0 1977 Pyruvate carboxylase: isolation of the biotin-containing tryptic peptide and the determination of its primary sequency. Biotin 39-45 pyruvate carboxylase Homo sapiens 0-20 35104-0 1979 Biotin subunits of acetyl CoA carboxylase and pyruvate carboxylase from a thermophilic Bacillus. Biotin 0-6 pyruvate carboxylase Homo sapiens 46-66 28791550-4 2017 The prodrug is a conjugate of doxorubicin and photocleavable biotin (DOX-PCB). Biotin 61-67 pyruvate carboxylase Homo sapiens 73-76 4690959-2 1973 The effect of this biotin deficiency on the growth rate is severe only if the functioning of pyruvate carboxylase is essential for the utilization of the particular growth substrate. Biotin 19-25 pyruvate carboxylase Homo sapiens 93-113 5451101-0 1970 Mechanism of pyruvate carboxylase formation from the apo-enzyme and biotin in a thermophilic bacillus. Biotin 68-74 pyruvate carboxylase Homo sapiens 13-33 31697914-1 2019 Pyruvate carboxylase (PC) is a biotin-containing enzyme that converts pyruvate to oxaloacetate. Biotin 31-37 pyruvate carboxylase Homo sapiens 0-20 31697914-1 2019 Pyruvate carboxylase (PC) is a biotin-containing enzyme that converts pyruvate to oxaloacetate. Biotin 31-37 pyruvate carboxylase Homo sapiens 22-24 30045381-1 2018 Pyruvate carboxylase (PC) is a biotin-containing enzyme that is responsible for the adenosine triphosphate-dependent carboxylation of pyruvate to oxaloacetate, a key intermediate in the tricarboxylic acid cycle. Biotin 31-37 pyruvate carboxylase Homo sapiens 0-20 30045381-1 2018 Pyruvate carboxylase (PC) is a biotin-containing enzyme that is responsible for the adenosine triphosphate-dependent carboxylation of pyruvate to oxaloacetate, a key intermediate in the tricarboxylic acid cycle. Biotin 31-37 pyruvate carboxylase Homo sapiens 22-24 182141-1 1976 Biotin-mediated restoration of hepatic gluconeogenesis in vitro and its relationship to pyruvate carboxylase activity. Biotin 0-6 pyruvate carboxylase Homo sapiens 88-108 182141-4 1976 Incubation of affected slices in a biotin-containing nutrient medium restores both gluconeogenesis and pyruvate carboxylase actiivity (the latter to approx. Biotin 35-41 pyruvate carboxylase Homo sapiens 103-123 5810685-0 1969 Regulation of pyruvate carboxylase formation from the apo-enzyme and biotin in a thermophilic bacillus. Biotin 69-75 pyruvate carboxylase Homo sapiens 14-34 33572391-2 2021 BTD recycles the vitamin biotin, a coenzyme essential for the function of four biotin-dependent carboxylases, including propionyl-CoA carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and acetyl-CoA carboxylase. Biotin 17-31 pyruvate carboxylase Homo sapiens 181-201 33572391-2 2021 BTD recycles the vitamin biotin, a coenzyme essential for the function of four biotin-dependent carboxylases, including propionyl-CoA carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and acetyl-CoA carboxylase. Biotin 25-31 pyruvate carboxylase Homo sapiens 181-201 28271481-3 2017 Pyruvate carboxylase oligomers arrange in tetramers and covalently attached biotins mediate the transfer of carboxyl groups between distant active sites. Biotin 76-83 pyruvate carboxylase Homo sapiens 0-20 21958016-0 2011 Interaction between the biotin carboxyl carrier domain and the biotin carboxylase domain in pyruvate carboxylase from Rhizobium etli. Biotin 24-30 pyruvate carboxylase Homo sapiens 92-112 24812517-1 2013 Biotin enzymes such as pyruvate carboxylase and acetyl-CoA carboxylase are involved with the most basic metabolism. Biotin 0-6 pyruvate carboxylase Homo sapiens 23-43 23698000-0 2013 A substrate-induced biotin binding pocket in the carboxyltransferase domain of pyruvate carboxylase. Biotin 20-26 pyruvate carboxylase Homo sapiens 79-99 23698000-2 2013 Pyruvate carboxylase (PC), a multifunctional biotin-dependent enzyme, catalyzes the bicarbonate- and MgATP-dependent carboxylation of pyruvate to oxaloacetate, an important anaplerotic reaction in mammalian tissues. Biotin 45-51 pyruvate carboxylase Homo sapiens 0-20 23698000-2 2013 Pyruvate carboxylase (PC), a multifunctional biotin-dependent enzyme, catalyzes the bicarbonate- and MgATP-dependent carboxylation of pyruvate to oxaloacetate, an important anaplerotic reaction in mammalian tissues. Biotin 45-51 pyruvate carboxylase Homo sapiens 22-24 23098296-0 2012 Localization of inhibitory antibodies to the biotin domain of human pyruvate carboxylase. Biotin 45-51 pyruvate carboxylase Homo sapiens 68-88 28649521-1 2015 Pyruvate carboxylase (PC) is a biotin-containing mitochondrial enzyme that catalyzes the conversion of pyruvate to oxaloacetate, thereby being involved in gluconeogenesis and in energy production through replenishment of the tricarboxylic acid (TCA) cycle with oxaloacetate. Biotin 31-37 pyruvate carboxylase Homo sapiens 0-20 28649521-1 2015 Pyruvate carboxylase (PC) is a biotin-containing mitochondrial enzyme that catalyzes the conversion of pyruvate to oxaloacetate, thereby being involved in gluconeogenesis and in energy production through replenishment of the tricarboxylic acid (TCA) cycle with oxaloacetate. Biotin 31-37 pyruvate carboxylase Homo sapiens 22-24 22869039-1 2013 Biotin-dependent carboxylases include acetyl-CoA carboxylase (ACC), propionyl-CoA carboxylase (PCC), 3-methylcrotonyl-CoA carboxylase (MCC), geranyl-CoA carboxylase, pyruvate carboxylase (PC), and urea carboxylase (UC). Biotin 0-6 pyruvate carboxylase Homo sapiens 166-186 18297087-1 2008 Pyruvate carboxylase (PC) catalyzes the biotin-dependent production of oxaloacetate and has important roles in gluconeogenesis, lipogenesis, insulin secretion and other cellular processes. Biotin 40-46 pyruvate carboxylase Homo sapiens 0-20 19306334-11 2009 Finally, although most PC mutations are suggested to interfere with biotin metabolism, none of the PC-deficient patients was biotin-responsive. Biotin 68-74 pyruvate carboxylase Homo sapiens 23-25 18613815-1 2008 PC (pyruvate carboxylase) is a biotin-containing enzyme that catalyses the HCO(3)(-)- and MgATP-dependent carboxylation of pyruvate to form oxaloacetate. Biotin 31-37 pyruvate carboxylase Homo sapiens 0-2 18613815-1 2008 PC (pyruvate carboxylase) is a biotin-containing enzyme that catalyses the HCO(3)(-)- and MgATP-dependent carboxylation of pyruvate to form oxaloacetate. Biotin 31-37 pyruvate carboxylase Homo sapiens 4-24 20596761-5 2010 The PCB included an ortho-nitrophenyl group for photo cleavability and a water-soluble glycol spacer arm ending in a biotin group for enhanced membrane interaction. Biotin 117-123 pyruvate carboxylase Homo sapiens 4-7 17717183-0 2007 Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme. Biotin 47-53 pyruvate carboxylase Homo sapiens 23-43 10858297-0 2000 Effects of Mg(2+) on the pre-steady-state kinetics of the biotin carboxylation reaction of pyruvate carboxylase. Biotin 58-64 pyruvate carboxylase Homo sapiens 91-111 10858297-1 2000 The effects of Mg(2+) concentration on the kinetics of both ATP cleavage and carboxyenzyme formation in the approach to steady state of the biotin carboxylation reaction of pyruvate carboxylase have been studied. Biotin 140-146 pyruvate carboxylase Homo sapiens 173-193 9597748-1 1998 Pyruvate carboxylase [EC 6.4.1.1] is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. Biotin 63-69 pyruvate carboxylase Homo sapiens 0-20 10846444-2 2000 In humans, biotin is involved in important metabolic pathways such as gluconeogenesis, fatty acid synthesis, and amino acid catabolism by acting a as prosthetic group for pyruvate carboxylase, propionyl-CoA carboxylase, beta-methylcrotinyl-CoA carboxylase, and acetyl-CoA carboxylase. Biotin 11-17 pyruvate carboxylase Homo sapiens 171-191 10229653-1 1999 Pyruvate carboxylase (PC; EC 6.4.1.1), a member of the biotin-dependent enzyme family, catalyses the ATP-dependent carboxylation of pyruvate to oxaloacetate. Biotin 55-61 pyruvate carboxylase Homo sapiens 0-20 10229653-1 1999 Pyruvate carboxylase (PC; EC 6.4.1.1), a member of the biotin-dependent enzyme family, catalyses the ATP-dependent carboxylation of pyruvate to oxaloacetate. Biotin 55-61 pyruvate carboxylase Homo sapiens 22-24 9831077-12 1998 Gel electrophoresis of SDS-solubilized biotin-containing membranes, followed by Western blot analysis using avidin-linked peroxidase, resulted in three stained bands with molecular weights similar to those of the mitochondrial carboxylases: propionyl carboxylase, methylmalonyl carboxylase, and pyruvate carboxylase. Biotin 39-45 pyruvate carboxylase Homo sapiens 295-315 9649849-0 1998 The role of Mg2+ in biotin carboxylation in pyruvate carboxylase. Biotin 20-26 pyruvate carboxylase Homo sapiens 44-64 8620009-0 1996 Effects of acetyl CoA on the pre-steady-state kinetics of the biotin carboxylation reaction of pyruvate carboxylase. Biotin 62-68 pyruvate carboxylase Homo sapiens 95-115 9056115-0 1997 Lack of mutations in the biotin-binding region of the pyruvate carboxylase (PC) gene in a family with partial PC deficiency. Biotin 25-31 pyruvate carboxylase Homo sapiens 54-74 9056115-0 1997 Lack of mutations in the biotin-binding region of the pyruvate carboxylase (PC) gene in a family with partial PC deficiency. Biotin 25-31 pyruvate carboxylase Homo sapiens 76-78 7822739-3 1994 The lymphocyte pyruvate carboxylase activity of both heterozygous parents slightly increased after receiving oral biotin for 1 month, but a definitive enzymatic response to biotin was not confirmed in our patient. Biotin 114-120 pyruvate carboxylase Homo sapiens 15-35 8251494-0 1993 Locus of action of acetyl CoA in the biotin-carboxylation reaction of pyruvate carboxylase. Biotin 37-43 pyruvate carboxylase Homo sapiens 70-90 2085665-3 1990 It is suggested that in diabetes may exist a deficiency, inactivity or unavailability of Biotin, resulting in disordered activity of biotin-dependent enzyme, pyruvate carboxylase, leading to accumulation of pyruvate and/or depletion of aspartate, both of which play a significant role in nervous system metabolism. Biotin 89-95 pyruvate carboxylase Homo sapiens 158-178 2085665-3 1990 It is suggested that in diabetes may exist a deficiency, inactivity or unavailability of Biotin, resulting in disordered activity of biotin-dependent enzyme, pyruvate carboxylase, leading to accumulation of pyruvate and/or depletion of aspartate, both of which play a significant role in nervous system metabolism. Biotin 133-139 pyruvate carboxylase Homo sapiens 158-178 8449348-0 1993 The effects of pH on the binding of ATP and phosphate in the biotin carboxylation reaction of pyruvate carboxylase. Biotin 61-67 pyruvate carboxylase Homo sapiens 94-114