PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 561660-4 1977 If histone H1 is selectively removed with 50-100 mM NaCl in 50 mM sodium phosphate buffer (pH 7.0) in the presence of the ion-exchange resin AG 50 W - X2, chromatin appears as "beads-on-a-string" with the nucleosomes separated from each other by distances of about 150-200 A. sodium phosphate 66-82 H1.0 linker histone Homo sapiens 3-13 7207472-2 1980 We have not modified this procedure so that the nucleosome is treated with the cation exchange resin in two steps, first in 50 mM sodium phosphate buffer and then in 50 mM sodium phosphate and 50 mM NaCl whereby histone H 1 is selectively removed without a release of free DNA at low resin concentrations. sodium phosphate 130-146 H1.0 linker histone Homo sapiens 212-223 7207472-2 1980 We have not modified this procedure so that the nucleosome is treated with the cation exchange resin in two steps, first in 50 mM sodium phosphate buffer and then in 50 mM sodium phosphate and 50 mM NaCl whereby histone H 1 is selectively removed without a release of free DNA at low resin concentrations. sodium phosphate 172-188 H1.0 linker histone Homo sapiens 212-223